##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513106_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2216105 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30173299550337 31.0 31.0 34.0 26.0 34.0 2 30.470470938876993 31.0 31.0 34.0 26.0 34.0 3 30.47899174452474 31.0 31.0 34.0 26.0 34.0 4 33.683066009958914 37.0 35.0 37.0 28.0 37.0 5 33.611124021650596 37.0 35.0 37.0 28.0 37.0 6 33.57096978708139 37.0 35.0 37.0 28.0 37.0 7 33.47972546427177 37.0 35.0 37.0 28.0 37.0 8 33.47616922483366 37.0 35.0 37.0 28.0 37.0 9 35.01208020378096 39.0 35.0 39.0 28.0 39.0 10 34.911332269905984 39.0 35.0 39.0 27.0 39.0 11 34.9143519824196 39.0 35.0 39.0 27.0 39.0 12 34.830603694319535 39.0 35.0 39.0 27.0 39.0 13 34.79786201466086 39.0 35.0 39.0 27.0 39.0 14 35.95350130070552 40.0 36.0 41.0 27.0 41.0 15 35.93026142714357 40.0 36.0 41.0 27.0 41.0 16 35.89425636420657 40.0 36.0 41.0 27.0 41.0 17 35.7921249218787 40.0 36.0 41.0 27.0 41.0 18 35.713109712761806 40.0 36.0 41.0 27.0 41.0 19 35.78554220129461 40.0 36.0 41.0 27.0 41.0 20 35.67699544922285 40.0 36.0 41.0 26.0 41.0 21 35.692627831262506 40.0 36.0 41.0 27.0 41.0 22 35.656315021174535 40.0 36.0 41.0 26.0 41.0 23 35.58377152707114 40.0 36.0 41.0 26.0 41.0 24 35.44537691129256 40.0 36.0 41.0 25.0 41.0 25 35.4197508692052 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7187852589541386 1101 2 0.7155398247188494 1101 3 0.8100968389580174 1101 4 0.9511200274440199 1101 5 0.8402938425620903 1101 6 0.7757923355335805 1101 7 0.6111698175594213 1101 8 0.6299886407962489 1101 9 0.652050189796384 1101 10 0.667023230330372 1101 11 0.6955132632787837 1101 12 0.6781993391926235 1101 13 0.7166085177769475 1101 14 0.6719818480188664 1101 15 0.581230599918392 1101 16 0.7056963255150492 1101 17 0.7172317636909753 1101 18 0.6054022418454323 1101 19 0.6521550459060492 1101 20 0.7495584848129582 1101 21 0.7762511569426138 1101 22 0.8412598113195031 1101 23 0.754783646709221 1101 24 0.7966642932953008 1101 25 0.8029691284858202 1102 1 0.8916456115714553 1102 2 0.8813469090689736 1102 3 0.8221403935595717 1102 4 1.100493655404371 1102 5 0.9407346454584413 1102 6 0.8009859837713691 1102 7 0.804453011542698 1102 8 0.8848443797349219 1102 9 0.8912228748935931 1102 10 0.9394440458282318 1102 11 0.96084994743088 1102 12 0.8883964378444631 1102 13 0.8849092607411038 1102 14 1.0544755597642776 1102 15 0.9959772445157427 1102 16 1.0037065398657674 1102 17 1.0072086702947658 1102 18 1.0004316766276062 1102 19 1.007988605308178 1102 20 1.0992922497397544 1102 21 1.1180505322426484 1102 22 1.1446854411450786 1102 23 1.0596131168706364 1102 24 1.0728080555467727 1102 25 1.1319762655922716 1103 1 0.30385885511162414 1103 2 0.39925384539422737 1103 3 0.6010441538861642 1103 4 -0.22205994710547117 1103 5 0.26202747418412997 1103 6 0.3472114485949831 1103 7 0.5196927306187362 1103 8 0.3896897282003948 1103 9 0.6725731754304789 1103 10 0.5833560490353094 1103 11 0.5574437253405975 1103 12 0.6473992237414308 1103 13 0.6223318905720205 1103 14 0.7258523079273047 1103 15 0.6523281000753585 1103 16 0.6374171459379099 1103 17 0.6660499128606361 1103 18 0.7315341040116081 1103 19 0.7006830630731713 1103 20 0.8257780773135366 1103 21 0.7765251426484099 1103 22 0.8654400246813978 1103 23 0.7209571948097846 1103 24 0.8191715465953635 1103 25 0.7547604562612946 1104 1 0.5950530606152142 1104 2 0.5903467244087466 1104 3 0.7286191913216875 1104 4 0.4938487882100091 1104 5 0.5368552834844706 1104 6 0.6131328834759131 1104 7 0.45517568182936685 1104 8 0.53040186645935 1104 9 0.4912892215657223 1104 10 0.44606810755465176 1104 11 0.5622580440331362 1104 12 0.691322803331964 1104 13 0.61483978233138 1104 14 0.47945921483893983 1104 15 0.6186852118558406 1104 16 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-1.1640242388019573 2214 21 -1.2442444018734378 2214 22 -1.3341880185310302 2214 23 -1.3252602297136065 2214 24 -1.1708632224039803 2214 25 -1.1870184614203794 2215 1 -0.4584962185583059 2215 2 -0.35549203809733143 2215 3 -0.3282530737275984 2215 4 0.0698972235485229 2215 5 -0.2866761193568266 2215 6 -0.45891401824980704 2215 7 -0.634224326964862 2215 8 -0.6527789755282356 2215 9 -0.7839389144082887 2215 10 -0.829040926683696 2215 11 -0.8831460683141259 2215 12 -0.984197092351998 2215 13 -0.9603760480449708 2215 14 -1.101835707314713 2215 15 -1.159349327963838 2215 16 -1.1735544364156567 2215 17 -1.028866743794893 2215 18 -1.0518844084439394 2215 19 -1.1326905560528857 2215 20 -1.0048382567179246 2215 21 -1.1549301490472672 2215 22 -1.283607759093421 2215 23 -1.1660040068358057 2215 24 -1.1375325641476266 2215 25 -1.3158106032936487 2216 1 -2.3837819403638285 2216 2 -1.8695935657442746 2216 3 -3.502912827708691 2216 4 -1.996969050973295 2216 5 -2.2668350147517806 2216 6 -2.131216163052187 2216 7 -2.067398644436679 2216 8 -2.2886809702188025 2216 9 -2.4120160821451435 2216 10 -2.2905906190094782 2216 11 -2.3207546027938832 2216 12 -2.359191690998003 2216 13 -2.454526872704058 2216 14 -2.7617569012326157 2216 15 -2.8746937824558927 2216 16 -2.8191581794688645 2216 17 -2.7674049396824216 2216 18 -2.8769437840552783 2216 19 -2.816165974458137 2216 20 -2.7511568902500017 2216 21 -2.9038340997481598 2216 22 -2.971334599593334 2216 23 -3.0649023139401663 2216 24 -2.838438184619818 2216 25 -2.9469624181354988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66695.0 3 1.0 4 1104.0 5 8468.0 6 9266.0 7 7365.0 8 5925.0 9 5123.0 10 4740.0 11 4087.0 12 3752.0 13 3626.0 14 3606.0 15 3645.0 16 3642.0 17 3742.0 18 4285.0 19 4980.0 20 6314.0 21 8107.0 22 10471.0 23 12387.0 24 14295.0 25 15823.0 26 17785.0 27 19204.0 28 21682.0 29 26022.0 30 32631.0 31 41216.0 32 52320.0 33 68129.0 34 90697.0 35 123344.0 36 180912.0 37 302889.0 38 1027825.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.84272785879685 18.66817784392318 13.488206397759091 28.000887899520876 2 14.393454688300752 19.90066441957543 38.096052991016904 27.609827901106915 3 17.159791616371965 23.553757606250606 29.222306704781587 30.064144072595838 4 10.728914018063223 16.432885625906714 36.54158986149122 36.29661049453884 5 13.516639843011664 37.23508443809783 35.17176917876289 14.076506540127617 6 33.956691620624355 36.03207162536182 16.769666470857587 13.241570283156237 7 29.083866332983465 30.776273716491414 21.079891736467285 19.059968214057836 8 26.359216201723516 35.83242908496083 19.219731451774525 18.58862326154113 9 26.33836079070737 14.731470222087411 18.723825670274987 40.20634331693023 10 14.587037394699365 28.770155358486953 33.782348301200884 22.8604589456128 11 35.94856361791122 21.813576508143658 22.79159158833927 19.446268285605857 12 23.244336576881665 24.295744456167874 30.640520315575802 21.819398651374662 13 29.191353631663336 20.807360216759413 25.14606846026897 24.85521769130828 14 21.725084778650448 20.414518527209268 26.7647383989188 31.095658295221483 15 24.143191900376472 28.76202053785406 22.6092830522769 24.485504509492564 16 23.823471008737094 27.384377933424798 25.272312080294608 23.519838977543507 17 23.023111801976732 27.358639596529667 26.257482246167818 23.360766355325786 18 22.97140545341336 27.123978935514735 27.050696633393883 22.85391897767802 19 24.00846230127833 26.458097209941172 26.598473553954392 22.934966934826107 20 24.304398934166024 26.270867129490703 26.56557338212765 22.859160554215617 21 24.138747938387397 25.79810072176183 26.337289974978624 23.725861364872152 22 23.895573540062408 26.183867641650554 26.282554301353052 23.638004516933993 23 23.526420287893146 26.167095347682867 26.346780379946754 23.959703984477233 24 23.609618875673988 26.260535959326763 26.602487882998112 23.527357282001134 25 23.66647789703105 25.998813233127493 26.435299771445848 23.89940909839561 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 135.0 2 242.0 3 349.0 4 349.0 5 349.0 6 880.0 7 1411.0 8 1411.0 9 1411.0 10 2127.0 11 2843.0 12 2843.0 13 2843.0 14 5012.0 15 7181.0 16 7181.0 17 7181.0 18 13350.0 19 19519.0 20 19519.0 21 19519.0 22 30934.0 23 42349.0 24 42349.0 25 42349.0 26 59857.0 27 77365.0 28 77365.0 29 77365.0 30 99655.0 31 121945.0 32 121945.0 33 121945.0 34 146138.0 35 170331.0 36 170331.0 37 170331.0 38 194332.0 39 218333.0 40 218333.0 41 218333.0 42 238966.5 43 259600.0 44 259600.0 45 259600.0 46 276729.5 47 293859.0 48 293859.0 49 293859.0 50 294660.5 51 295462.0 52 295462.0 53 295462.0 54 274919.0 55 254376.0 56 254376.0 57 254376.0 58 223644.0 59 192912.0 60 192912.0 61 192912.0 62 160640.5 63 128369.0 64 128369.0 65 128369.0 66 100499.0 67 72629.0 68 72629.0 69 72629.0 70 53749.5 71 34870.0 72 34870.0 73 34870.0 74 24612.5 75 14355.0 76 14355.0 77 14355.0 78 9904.0 79 5453.0 80 5453.0 81 5453.0 82 3643.0 83 1833.0 84 1833.0 85 1833.0 86 1165.5 87 498.0 88 498.0 89 498.0 90 306.5 91 115.0 92 115.0 93 115.0 94 64.0 95 13.0 96 13.0 97 13.0 98 6.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18694962558182038 2 0.06272265980176932 3 0.0 4 0.0 5 0.018004562058205723 6 0.3687550905755819 7 0.00139885068622651 8 0.028744125391170546 9 0.03027834872445123 10 0.0031135708822461034 11 0.0038806825488864475 12 0.009882203234955023 13 0.008663849411467417 14 0.0018049686273890451 15 9.024843136945226E-5 16 0.011957917156452424 17 0.023780461665850672 18 0.06236166607629151 19 0.028518504312746912 20 0.0 21 0.0 22 0.0 23 2.2562107842363064E-4 24 1.8049686273890453E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2216105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.80682512628708 #Duplication Level Percentage of deduplicated Percentage of total 1 77.99702224899346 52.887304480086215 2 13.776120703073303 18.682300148638287 3 4.24460107379201 8.634387682243956 4 1.7327303694525804 4.699637806099117 5 0.8324381621056051 2.822249444317129 6 0.4409559666190171 1.7939894470157147 7 0.263405443939764 1.2502480812174916 8 0.15508039307098642 0.841240727478419 9 0.1087599653523078 0.6637201156246479 >10 0.423120678488448 4.717003688789775 >50 0.01615930437447329 0.734399762649452 >100 0.007806763004061779 0.9731711338992186 >500 0.0013325390621374905 0.618978593269497 >1k 4.663886717476308E-4 0.681368888671131 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3383 0.1526552216614285 No Hit TATCAACGCAGAGTACTTTTTTTTT 2556 0.11533749529015998 No Hit GTACATGGGAAGCAGTGGTATCAAC 2433 0.10978721676093868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.8049686273890453E-4 0.0 0.0 0.0 0.0 7 1.8049686273890453E-4 0.0 0.0 0.0 0.0 8 1.8049686273890453E-4 0.0 0.0 0.0 0.0 9 1.8049686273890453E-4 0.0 0.0 0.0 0.0 10 1.8049686273890453E-4 4.512421568472613E-5 0.0 0.0 0.0 11 1.8049686273890453E-4 4.512421568472613E-5 0.0 0.0 0.0 12 1.8049686273890453E-4 4.512421568472613E-5 0.0 0.0 0.0 13 1.8049686273890453E-4 4.512421568472613E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTACG 40 0.005208205 14.279452 4 TACGCTG 65 7.887956E-4 11.716473 5 TTAGACT 235 0.0 10.937452 4 GTCCTAA 430 0.0 10.857545 1 ACAGTCG 70 0.0015081513 10.842995 8 CTAGGAC 240 0.0 10.709588 3 ATTAGGC 125 2.1783853E-7 10.66199 3 TAGGACG 585 0.0 10.577372 4 GTAGGAC 670 0.0 10.372139 3 TGGCGAG 625 0.0 10.173715 18 GTACCGT 75 0.0026033532 10.154276 6 CCTTACG 85 6.4363313E-4 10.08555 2 GGCCGCG 85 6.674834E-4 10.045715 7 GGCGAGG 340 0.0 10.045035 19 GGACCGT 95 1.6106272E-4 10.020668 6 TACACCG 95 1.6106272E-4 10.020668 5 CCGTGCA 210 7.2759576E-12 9.939412 9 CCAGGAC 730 0.0 9.910852 3 ACCGTGC 125 2.6140406E-6 9.8671255 8 TAGGACA 310 0.0 9.826719 4 >>END_MODULE