FastQCFastQC Report
Thu 26 May 2016
SRR1513105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086901
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC87950.42143829534798244No Hit
CCCATGTACTCTGCGTTGATACCAC69570.3333651189011841No Hit
GAGTACATGGGAAGCAGTGGTATCA36120.1730796046386484No Hit
GCGTTGATACCACTGCTTCCCATGT24090.11543432103391583No Hit
CATGTACTCTGCGTTGATACCACTG23430.11227173689600034No Hit
GTATCAACGCAGAGTACATGGGAAG22850.10949249628995338No Hit
GTATCAACGCAGAGTACTTTTTTTT21040.10081934888142755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3950.013.2289674
ACCGTCC655.4621807E-513.1518058
TATCGAC1051.9965228E-812.665915519
GTAGGAC8350.012.6292053
AGGACGT6050.011.6208165
GTACTAG1251.8028004E-811.4127291
GGACGTG6050.011.1478316
GTCCTAG4100.011.1343711
TAGGACG6400.011.1337544
CTAATAC2050.011.1223583
GCGTTTA2050.011.12102513
ACGGGCG600.00587939511.08267616
GACGTGG3450.011.0127847
GGTATCG1053.4699278E-610.856517
TTAGGAC4250.010.7298053
ACCGAGC803.7772534E-410.6858428
TGTAGGA10400.010.5973042
GTATTAG1803.45608E-1110.5673431
GTATCGA1002.4010236E-510.44938118
AGACCCG1106.0411658E-610.3645125
Produced by FastQC (version 0.11.5)