##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513105_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086901 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.320768929623398 31.0 30.0 34.0 25.0 34.0 2 29.012299577220002 31.0 30.0 34.0 16.0 34.0 3 29.38746543319496 31.0 30.0 34.0 23.0 34.0 4 32.26993949401529 35.0 33.0 37.0 19.0 37.0 5 32.489685902685366 35.0 33.0 37.0 22.0 37.0 6 32.94874169881561 37.0 33.0 37.0 26.0 37.0 7 32.95571519683972 37.0 35.0 37.0 26.0 37.0 8 32.933591004077336 37.0 35.0 37.0 26.0 37.0 9 34.30734184324029 39.0 35.0 39.0 26.0 39.0 10 33.814585358864655 39.0 34.0 39.0 22.0 39.0 11 34.18180689932105 39.0 35.0 39.0 26.0 39.0 12 34.02598350376947 39.0 34.0 39.0 25.0 39.0 13 34.107801472135 39.0 35.0 39.0 25.0 39.0 14 34.41374267394572 39.0 33.0 41.0 17.0 41.0 15 34.95872683946196 40.0 34.0 41.0 24.0 41.0 16 34.54412643436368 39.0 34.0 41.0 17.0 41.0 17 34.07908568734214 39.0 33.0 41.0 16.0 41.0 18 33.493687529978665 39.0 32.0 41.0 10.0 41.0 19 34.387340846547104 39.0 34.0 41.0 18.0 41.0 20 34.64238025665808 39.0 34.0 41.0 18.0 41.0 21 34.66763205346109 40.0 34.0 41.0 18.0 41.0 22 34.254828571168446 39.0 34.0 41.0 11.0 41.0 23 32.569890953140565 39.0 31.0 41.0 7.0 41.0 24 33.659296727540024 39.0 33.0 41.0 2.0 41.0 25 33.391687962198496 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.752212252350315 1101 2 1.1216524642616008 1101 3 0.8586430015076978 1101 4 0.87838569445762 1101 5 1.0928151536695623 1101 6 0.8351934944469406 1101 7 0.7946790110169246 1101 8 0.824796107278047 1101 9 0.8701312646817172 1101 10 1.1638125006013453 1101 11 0.9556850315162748 1101 12 0.9581631824733279 1101 13 0.8389104264401794 1101 14 -0.013366024808036059 1101 15 0.6322335379731712 1101 16 1.2846549415918318 1101 17 1.8592979173050708 1101 18 -4.244455650812956 1101 19 -0.6310094273087614 1101 20 0.33258107694092587 1101 21 0.6603750830881552 1101 22 1.2144184051385665 1101 23 3.05566904162729 1101 24 1.9870858894630317 1101 25 2.2279381903422717 1102 1 1.000550129616304 1102 2 1.34055213824022 1102 3 0.9998342781856202 1102 4 1.5655596666467986 1102 5 1.4400485492858905 1102 6 1.02529548734843 1102 7 0.9440568754045771 1102 8 0.854037123946334 1102 9 1.0142554480210393 1102 10 1.3614767083498407 1102 11 0.9733552978416427 1102 12 1.1159105233598225 1102 13 1.0891038328775977 1102 14 2.0167272042437148 1102 15 1.5456547258142592 1102 16 1.7271650645068775 1102 17 1.4686002500628916 1102 18 1.8234741622712036 1102 19 1.5075788214179369 1102 20 1.4881381754277783 1102 21 1.4381361972079816 1102 22 1.7920867376567173 1102 23 3.374046844812007 1102 24 2.284813203101365 1102 25 2.5524672491387577 1103 1 0.972987016631194 1103 2 1.30368048397726 1103 3 1.133369859803043 1103 4 1.5151051200285721 1103 5 1.4251835564157815 1103 6 0.91358735978352 1103 7 0.8130542615375163 1103 8 0.8234617915807192 1103 9 0.9585368137857841 1103 10 1.3611408225919632 1103 11 1.0607342368829862 1103 12 1.1647025378807356 1103 13 0.9632603423282191 1103 14 1.9387172249549636 1103 15 1.4149849255792404 1103 16 1.562435117168235 1103 17 2.0758520030946954 1103 18 2.0715170901095306 1103 19 1.5920499933571364 1103 20 1.540994100406465 1103 21 1.4911042547796214 1103 22 1.7354500498179846 1103 23 3.184935482810616 1103 24 2.0929901780302558 1103 25 2.278332244568965 1104 1 0.6309120352368467 1104 2 1.0817982596066997 1104 3 0.8033073299783702 1104 4 1.4195662731586438 1104 5 1.1260318752313552 1104 6 0.7170884365624346 1104 7 0.5656715389558187 1104 8 0.6365601703546986 1104 9 0.7824835855506862 1104 10 1.1123616253176607 1104 11 0.6913859662814019 1104 12 0.8758607366064055 1104 13 0.7214867601283288 1104 14 1.6067928937076061 1104 15 0.9760962702053604 1104 16 1.455949471335778 1104 17 1.7984505141480582 1104 18 2.404193064959138 1104 19 1.4163097851221949 1104 20 0.9981343437510759 1104 21 1.111964342751591 1104 22 1.3922930972382872 1104 23 2.8762779825695475 1104 24 1.9847263016756287 1104 25 2.0633770093960067 1105 1 0.4752593855102951 1105 2 0.8847497628631338 1105 3 0.6348928388754729 1105 4 0.7244598969918741 1105 5 0.817514311448825 1105 6 0.47232817465071975 1105 7 0.43346481924578484 1105 8 0.29298673834686895 1105 9 0.5398500708247056 1105 10 0.9304513462821404 1105 11 0.587301672543667 1105 12 0.5210691279488415 1105 13 0.5087267621075142 1105 14 1.3516111728090934 1105 15 0.7172189125293826 1105 16 1.04188243722691 1105 17 1.5764954706788288 1105 18 1.4043740048570186 1105 19 1.0469413090242057 1105 20 0.7755322824915254 1105 21 0.6844870912140877 1105 22 1.2189710116076355 1105 23 2.7622620773218998 1105 24 1.7399861226372977 1105 25 1.6399936564487234 1106 1 -0.5139479933979807 1106 2 -0.035617276787633045 1106 3 -0.263339318319467 1106 4 -2.5097491628310564 1106 5 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17 -1.1670327416144914 1107 18 0.6167536026567362 1107 19 0.6329917477696441 1107 20 0.3348013945958641 1107 21 0.36192172743768225 1107 22 0.5508752807644441 1107 23 2.4445148943086537 1107 24 1.4125250511521301 1107 25 1.140246308678826 1108 1 0.2858317926085796 1108 2 0.5830395188225808 1108 3 0.42257312350388787 1108 4 0.8774986195865111 1108 5 0.5517124250232825 1108 6 0.13819158848045987 1108 7 0.17508608579245788 1108 8 0.13976483365755854 1108 9 0.2709090033436823 1108 10 0.6908656956240051 1108 11 0.33398572660066606 1108 12 0.30810535833357733 1108 13 0.22184143332093242 1108 14 0.808067894386312 1108 15 0.4408813332088144 1108 16 0.8120559974000869 1108 17 0.8743678359291422 1108 18 0.6113855231604433 1108 19 0.6089750892025805 1108 20 0.4170649526875252 1108 21 0.5938345118079553 1108 22 0.9686406226704278 1108 23 2.555041319173604 1108 24 1.3011069092546634 1108 25 1.4675551332931036 1109 1 0.21466758879304848 1109 2 0.4548332896755234 1109 3 0.420717684049265 1109 4 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1.2726775889246156 1203 13 1.1939614188900052 1203 14 2.01517227809709 1203 15 1.5291655402040192 1203 16 1.8371384372395738 1203 17 2.102348012391545 1203 18 2.6567503787878763 1203 19 1.834340451178356 1203 20 1.6023109113948166 1203 21 1.5918360181942717 1203 22 1.8070474609047906 1203 23 3.5184908805493507 1203 24 2.375443592926196 1203 25 2.487371550372231 1204 1 0.6552315865417988 1204 2 1.0669663504889328 1204 3 0.7923815366515328 1204 4 1.3668668006327565 1204 5 1.0724615054015132 1204 6 0.6076642899135862 1204 7 0.5537678231239411 1204 8 0.5001249304829471 1204 9 0.6173221127987887 1204 10 0.9995186518616279 1204 11 0.6707726693123064 1204 12 0.7179940042651793 1204 13 0.6905375246338679 1204 14 1.5766187801240719 1204 15 0.751474494326871 1204 16 1.1959718797372574 1204 17 1.6073340112370502 1204 18 2.2745704453450486 1204 19 1.2803075108366713 1204 20 1.1785882583042238 1204 21 1.0721452923671464 1204 22 1.4772317832505735 1204 23 2.6815671153056257 1204 24 1.667431343363262 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0.8741701972481124 2212 20 0.5328679633537021 2212 21 0.38023607914492175 2212 22 0.5859343728311117 2212 23 2.022979834014457 2212 24 1.205562908431972 2212 25 1.2368984517707133 2213 1 -1.5582163953048713 2213 2 -3.8674637141130432 2213 3 -1.8295234315157494 2213 4 -3.787357204924067 2213 5 -1.4613462456652684 2213 6 -1.1097021502266635 2213 7 -0.9685984901927327 2213 8 -0.8288405988689078 2213 9 -0.8619814501632632 2213 10 -0.9933754231359799 2213 11 -1.0368648415896402 2213 12 -0.8871885924182834 2213 13 -1.3185142642614593 2213 14 -6.267855321526003 2213 15 -2.8417313715562216 2213 16 -1.9661941108621903 2213 17 -0.9377276737995288 2213 18 -0.19525842087168854 2213 19 -0.8885998357604592 2213 20 -0.9452862800091779 2213 21 -1.174046683407596 2213 22 -0.9463021849811 2213 23 -5.438696267996196 2213 24 -2.961493700000645 2213 25 -2.122654970190702 2214 1 -0.16630637370584012 2214 2 -0.019478745633580985 2214 3 -0.13296874031164663 2214 4 0.22203134934569135 2214 5 -0.055104477003176555 2214 6 -0.237008241855726 2214 7 -0.3752318510370216 2214 8 -0.5242152454701312 2214 9 -0.4797453358815673 2214 10 0.09635801321712023 2214 11 -0.31945215962219464 2214 12 -0.27340384519718697 2214 13 -0.3987423710976401 2214 14 0.17652102841341843 2214 15 -0.14614688071385729 2214 16 0.32461313095915045 2214 17 0.5403089216313148 2214 18 1.0754827946444934 2214 19 0.15645609343686573 2214 20 0.067835570132182 2214 21 -0.053921830474166654 2214 22 0.30632920912218964 2214 23 1.9860311100922061 2214 24 0.784472724920775 2214 25 0.5984558872732535 2215 1 -2.409738019109952 2215 2 -4.332077509105574 2215 3 -2.4942571950252095 2215 4 -3.887416322357989 2215 5 -5.040581810436134 2215 6 -2.3426286539072017 2215 7 -1.7877260139523905 2215 8 -2.170084457417012 2215 9 -1.8010894530341872 2215 10 -3.2583891709732526 2215 11 -2.2644808313819134 2215 12 -1.8467026852517279 2215 13 -1.9211194187469331 2215 14 -1.126144417913423 2215 15 -1.702548995139395 2215 16 -2.496947658775049 2215 17 -6.851922619172246 2215 18 -3.2306792988609345 2215 19 -2.2867177863098505 2215 20 -2.0705696449527125 2215 21 -2.0916770278143275 2215 22 -1.8103938048681343 2215 23 -6.399799250219356 2215 24 -3.7793074644809472 2215 25 -3.449660368773962 2216 1 -1.130560480881556 2216 2 -0.4908363126120747 2216 3 -0.8887976292343005 2216 4 -3.159517719818286 2216 5 -1.3272146815051613 2216 6 -1.1321515519413694 2216 7 -1.1073477619428402 2216 8 -0.9844475197421865 2216 9 -1.1127793362563665 2216 10 -0.6899179167325542 2216 11 -1.2487467772002816 2216 12 -1.9994149169712543 2216 13 -1.282571469367653 2216 14 -7.195031366672325 2216 15 -3.238390729868957 2216 16 -1.7828013109697451 2216 17 -0.9114177720141541 2216 18 -0.3329733192269657 2216 19 -0.7105183476141264 2216 20 -1.3076766977919192 2216 21 -1.5187384742645733 2216 22 -0.9399308171812919 2216 23 0.20457590958301353 2216 24 -0.43701019723071255 2216 25 -0.45571527445264337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94448.0 3 2.0 4 846.0 5 4877.0 6 5793.0 7 5321.0 8 4936.0 9 4671.0 10 4460.0 11 4365.0 12 4340.0 13 4237.0 14 4390.0 15 4424.0 16 4730.0 17 4878.0 18 5554.0 19 6605.0 20 8244.0 21 10236.0 22 12485.0 23 15647.0 24 18193.0 25 21422.0 26 24460.0 27 28424.0 28 31392.0 29 37894.0 30 44628.0 31 51268.0 32 66110.0 33 86050.0 34 112691.0 35 146838.0 36 206303.0 37 285995.0 38 709744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51505963403986 17.966829464876845 12.054423857454015 25.463687043629278 2 14.066464775950681 19.78219005564379 41.282592510739725 24.868752657665798 3 19.354433583488962 24.185971599602716 28.754539423478835 27.705055393429483 4 10.79817607413042 16.181278636647527 39.13287308587723 33.88767220334482 5 12.619303155964234 37.69162444030396 36.17157125210373 13.517501151628073 6 32.89512531321114 36.053117924591604 17.975347623621342 13.076409138575917 7 28.78919171770173 31.02042009629643 21.637892444382246 18.552495741619595 8 25.83474886597622 36.18829971203657 20.21318305041852 17.76376837156869 9 26.634957641945263 15.260020074180519 19.525352041114683 38.57967024275954 10 13.97150429573485 29.362185357735033 34.88703293500577 21.779277411524355 11 34.844569270438924 22.778773616556272 23.22186861957574 19.154788493429063 12 23.019089115288594 24.977797502680353 31.421290529442693 20.581822852588356 13 28.877796761663312 21.306070024178496 25.40970446643167 24.406428747726526 14 20.78797920487948 21.49736562229355 28.347035228720923 29.367619944106043 15 24.29125076326841 29.215278596563877 23.025639108483325 23.467831531684386 16 22.43525131409745 28.03395109213756 27.43306334843581 22.097734245329185 17 22.284458751496665 27.802105731374017 27.93166486603595 21.98177065109337 18 21.849821686437483 27.308886250756654 29.365734752045658 21.47555731076021 19 23.322813267554164 27.328283573933504 27.641584682375182 21.707318476137154 20 23.42548914538673 27.124071484101275 27.70924379575478 21.741195574757214 21 23.127417568986093 26.71704317232983 27.500730962617855 22.65480829606622 22 22.81799151246071 27.211129577104252 27.09349475604676 22.877384154388274 23 21.32475414479969 26.776347859764392 29.089450215190706 22.80944778024521 24 22.62249109569669 27.581960336901446 27.405024759475953 22.39052380792591 25 22.72993954686029 26.675594992723767 27.274662024192757 23.319803436223182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 176.0 1 176.0 2 306.0 3 436.0 4 436.0 5 436.0 6 884.5 7 1333.0 8 1333.0 9 1333.0 10 2270.0 11 3207.0 12 3207.0 13 3207.0 14 5686.0 15 8165.0 16 8165.0 17 8165.0 18 15062.5 19 21960.0 20 21960.0 21 21960.0 22 34474.5 23 46989.0 24 46989.0 25 46989.0 26 66562.5 27 86136.0 28 86136.0 29 86136.0 30 111114.0 31 136092.0 32 136092.0 33 136092.0 34 159926.0 35 183760.0 36 183760.0 37 183760.0 38 204837.5 39 225915.0 40 225915.0 41 225915.0 42 244224.0 43 262533.0 44 262533.0 45 262533.0 46 282832.0 47 303131.0 48 303131.0 49 303131.0 50 288098.5 51 273066.0 52 273066.0 53 273066.0 54 240275.5 55 207485.0 56 207485.0 57 207485.0 58 176568.0 59 145651.0 60 145651.0 61 145651.0 62 118885.5 63 92120.0 64 92120.0 65 92120.0 66 71030.0 67 49940.0 68 49940.0 69 49940.0 70 36710.0 71 23480.0 72 23480.0 73 23480.0 74 16627.5 75 9775.0 76 9775.0 77 9775.0 78 6775.5 79 3776.0 80 3776.0 81 3776.0 82 2510.0 83 1244.0 84 1244.0 85 1244.0 86 815.5 87 387.0 88 387.0 89 387.0 90 246.0 91 105.0 92 105.0 93 105.0 94 65.5 95 26.0 96 26.0 97 26.0 98 19.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12080113048007549 2 0.04571371617532408 3 0.03512385110745551 4 0.030284139017615112 5 0.03469258963410339 6 0.021515155726122132 7 0.020460961013483627 8 0.025156919278873317 9 0.03061956460800009 10 0.03651347141047898 11 0.021131812194253585 12 0.020317207189032925 13 0.016100428338478922 14 0.0215630736676057 15 0.022952693970629177 16 0.02338395544398129 17 0.02851117518272309 18 0.019550520125295835 19 0.03493217934152123 20 0.024294396332169087 21 0.029277862246460184 22 0.03756766612311748 23 0.029325780187943754 24 0.03981980937284519 25 0.031482087554704316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2086901.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.12310180001207 #Duplication Level Percentage of deduplicated Percentage of total 1 87.33842270866535 68.23148488321549 2 9.12223994052547 14.253153590356144 3 1.9661436831889196 4.608037293456559 4 0.6646868419227555 2.077095912666398 5 0.2994292830046339 1.1696172179037818 6 0.1609541703014522 0.7544543418958096 7 0.10022759150963555 0.5481063234274098 8 0.06381391545693604 0.3988272810799662 9 0.04348340529539329 0.3057352648652874 >10 0.21723443400320067 2.9210388913402903 >50 0.013354570484644394 0.7110121452893051 >100 0.007338007561304995 1.1218892147970525 >500 0.0016774208875994425 0.9018830324405126 >1k 8.697737935699702E-4 1.2331364872034158 >5k 1.242533990814243E-4 0.7645281200625107 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 8795 0.42143829534798244 No Hit CCCATGTACTCTGCGTTGATACCAC 6957 0.3333651189011841 No Hit GAGTACATGGGAAGCAGTGGTATCA 3612 0.1730796046386484 No Hit GCGTTGATACCACTGCTTCCCATGT 2409 0.11543432103391583 No Hit CATGTACTCTGCGTTGATACCACTG 2343 0.11227173689600034 No Hit GTATCAACGCAGAGTACATGGGAAG 2285 0.10949249628995338 No Hit GTATCAACGCAGAGTACTTTTTTTT 2104 0.10081934888142755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.791794148356822E-5 0.0 4 0.0 0.0 0.0 4.791794148356822E-5 0.0 5 0.0 0.0 0.0 4.791794148356822E-5 0.0 6 0.0 0.0 0.0 4.791794148356822E-5 4.791794148356822E-5 7 0.0 0.0 0.0 9.583588296713644E-5 4.791794148356822E-5 8 0.0 0.0 0.0 9.583588296713644E-5 4.791794148356822E-5 9 0.0 0.0 0.0 9.583588296713644E-5 4.791794148356822E-5 10 0.0 0.0 0.0 9.583588296713644E-5 4.791794148356822E-5 11 0.0 0.0 0.0 9.583588296713644E-5 4.791794148356822E-5 12 0.0 0.0 0.0 1.4375382445070466E-4 4.791794148356822E-5 13 0.0 0.0 0.0 1.9167176593427288E-4 4.791794148356822E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 395 0.0 13.228967 4 ACCGTCC 65 5.4621807E-5 13.151805 8 TATCGAC 105 1.9965228E-8 12.6659155 19 GTAGGAC 835 0.0 12.629205 3 AGGACGT 605 0.0 11.620816 5 GTACTAG 125 1.8028004E-8 11.412729 1 GGACGTG 605 0.0 11.147831 6 GTCCTAG 410 0.0 11.134371 1 TAGGACG 640 0.0 11.133754 4 CTAATAC 205 0.0 11.122358 3 GCGTTTA 205 0.0 11.121025 13 ACGGGCG 60 0.005879395 11.082676 16 GACGTGG 345 0.0 11.012784 7 GGTATCG 105 3.4699278E-6 10.8565 17 TTAGGAC 425 0.0 10.729805 3 ACCGAGC 80 3.7772534E-4 10.685842 8 TGTAGGA 1040 0.0 10.597304 2 GTATTAG 180 3.45608E-11 10.567343 1 GTATCGA 100 2.4010236E-5 10.449381 18 AGACCCG 110 6.0411658E-6 10.364512 5 >>END_MODULE