FastQCFastQC Report
Thu 26 May 2016
SRR1513105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086901
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC101700.48732546488788875No Hit
CCCATGTACTCTGCGTTGATACCAC94650.4535433161419732No Hit
GAGTACATGGGAAGCAGTGGTATCA39150.18759874090816958No Hit
GTATCAACGCAGAGTACTTTTTTTT34020.16301683692709906No Hit
CATGTACTCTGCGTTGATACCACTG26780.12832424729299569No Hit
GTATCAACGCAGAGTACATGGGAAG25790.12358037108612244No Hit
GCGTTGATACCACTGCTTCCCATGT24900.11931567429408488No Hit
ACGCAGAGTACATGGGAAGCAGTGG23740.11375719308199095No Hit
TATCAACGCAGAGTACTTTTTTTTT23200.11116962424187826No Hit
TATCAACGCAGAGTACATGGGAAGC23000.1102112654122069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCC1301.8189894E-1213.86538311
TAGGACC4100.012.3083174
CTACGCT550.003017451612.1183094
GTAGGAC7650.011.8241213
TGGCGAG6600.011.78837718
GACCGTG1052.7690476E-711.7461777
GGACGTG6200.011.517986
AGGACGT6400.011.4555895
GGCGAGG3250.011.38311919
ACGACAG855.2032614E-511.2017995
TAGGACG6400.011.1580424
TGTGCGC1803.6379788E-1211.0663910
GGCGAGA4100.010.87411819
TTAGACT2050.010.6826914
CCAACGA803.82384E-410.67167419
ATGGCGA7400.010.64549517
GGCGTGC1252.2826498E-710.6257728
TATGGCG7350.010.58928916
TAGGACA5050.010.5585274
TGTAGGA9550.010.4744922
Produced by FastQC (version 0.11.5)