##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513105_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2086901
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.683894444441783	31.0	31.0	34.0	27.0	34.0
2	30.802699792659066	31.0	31.0	34.0	27.0	34.0
3	30.810625899359863	33.0	31.0	34.0	27.0	34.0
4	34.06674873412778	37.0	35.0	37.0	30.0	37.0
5	34.01344721191853	37.0	35.0	37.0	30.0	37.0
6	33.98116920735579	37.0	35.0	37.0	30.0	37.0
7	33.90401509223485	37.0	35.0	37.0	30.0	37.0
8	33.899946859002895	37.0	35.0	37.0	29.0	37.0
9	35.47081006717616	39.0	35.0	39.0	30.0	39.0
10	35.38220068896416	39.0	35.0	39.0	30.0	39.0
11	35.39672509620725	39.0	35.0	39.0	30.0	39.0
12	35.31630297747713	39.0	35.0	39.0	29.0	39.0
13	35.29270626637296	39.0	35.0	39.0	29.0	39.0
14	36.48285951274162	40.0	37.0	41.0	29.0	41.0
15	36.47166588161106	40.0	37.0	41.0	29.0	41.0
16	36.42968449389789	40.0	37.0	41.0	29.0	41.0
17	36.34006883891473	40.0	37.0	41.0	28.0	41.0
18	36.263684285934026	40.0	36.0	41.0	28.0	41.0
19	36.33413180596492	40.0	37.0	41.0	28.0	41.0
20	36.233809366136676	40.0	37.0	41.0	27.0	41.0
21	36.24736822685887	40.0	37.0	41.0	28.0	41.0
22	36.22785220765144	40.0	37.0	41.0	28.0	41.0
23	36.16727530438674	40.0	37.0	41.0	28.0	41.0
24	36.03942496553502	40.0	37.0	41.0	27.0	41.0
25	36.0208395127512	40.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.47186131293761235
1101	2	0.36654143178957455
1101	3	0.4339111615017508
1101	4	0.702447272031705
1101	5	0.5075629786306521
1101	6	0.5069848395282079
1101	7	0.42212577451105204
1101	8	0.3544643018003626
1101	9	0.44850294868751917
1101	10	0.4943122611318955
1101	11	0.45668366131312865
1101	12	0.45686275958028233
1101	13	0.4697012219807988
1101	14	0.48601056905524587
1101	15	0.4475203519114501
1101	16	0.4172762130422285
1101	17	0.5874957580055877
1101	18	0.4466808471979178
1101	19	0.4472857645475372
1101	20	0.6721272602765183
1101	21	0.6508390448108159
1101	22	0.6799719686974086
1101	23	0.5280432786346267
1101	24	0.5855301930773535
1101	25	0.5905413332515934
1102	1	0.43369464447085804
1102	2	0.3400679195482148
1102	3	0.34470031428331893
1102	4	0.5649606762473027
1102	5	0.4373458213375514
1102	6	0.3253944785285796
1102	7	0.34257984890399484
1102	8	0.27472160102256993
1102	9	0.2976590938485515
1102	10	0.2609309426353903
1102	11	0.2672401914869482
1102	12	0.25907470588010284
1102	13	0.39554187523733475
1102	14	0.3030851610399239
1102	15	0.31048768651307057
1102	16	0.40293507813397156
1102	17	0.3297436340320914
1102	18	0.2788520961133685
1102	19	0.319111067694962
1102	20	0.4811632830933874
1102	21	0.3751328580163502
1102	22	0.38728212000238926
1102	23	0.310834863075911
1102	24	0.45988837223328005
1102	25	0.429508680196669
1103	1	0.13786479928040052
1103	2	0.26746846587459316
1103	3	0.47945348177306
1103	4	-0.49813310807410716
1103	5	0.07273773499033354
1103	6	0.15973930929284563
1103	7	0.2657342053064369
1103	8	0.25939783736121313
1103	9	0.45859445376089525
1103	10	0.4715543537703084
1103	11	0.5176486562176663
1103	12	0.5804759024731183
1103	13	0.5803005469525289
1103	14	0.6373590768995143
1103	15	0.6484107758428124
1103	16	0.5905354662458677
1103	17	0.6529046467071709
1103	18	0.5779455629817747
1103	19	0.48947426977171915
1103	20	0.7150672638004352
1103	21	0.6407002156553219
1103	22	0.6065126036830009
1103	23	0.6371058911372174
1103	24	0.6885221210716281
1103	25	0.798931811768135
1104	1	0.3031166122497133
1104	2	0.3007539148951075
1104	3	0.411013364773801
1104	4	0.19142028211052065
1104	5	0.205493819223868
1104	6	0.21786410685593438
1104	7	0.2572396435962503
1104	8	0.20550499107974218
1104	9	0.23090627706530853
1104	10	0.2142370785772698
1104	11	0.11777524882435841
1104	12	0.11676322813012519
1104	13	0.2034521589066287
1104	14	0.07448532582706235
1104	15	0.11837643265322129
1104	16	0.15612601333856446
1104	17	0.03578991968483791
1104	18	0.08648339364932411
1104	19	0.18852902849165076
1104	20	0.3138933151742833
1104	21	0.3135700848841694
1104	22	0.2656086039005814
1104	23	0.20331901671362118
1104	24	0.2690318046642872
1104	25	0.33652215116983797
1105	1	0.3190356365519307
1105	2	0.2745746767054378
1105	3	0.32499249992959633
1105	4	0.33719691204913715
1105	5	0.36897452074677517
1105	6	0.30269067135543537
1105	7	0.17665163258656236
1105	8	0.24549891770837462
1105	9	0.12004144256467697
1105	10	0.12582858323077062
1105	11	0.20849094616090724
1105	12	0.09186075619036416
1105	13	0.21398907016070012
1105	14	0.20402432201326093
1105	15	0.28884529498269984
1105	16	0.224257015765005
1105	17	0.21532129990214344
1105	18	0.15831672714435285
1105	19	0.11209312729938858
1105	20	0.3257783014315834
1105	21	0.22849486142582265
1105	22	0.30136873095629113
1105	23	0.2677310008530043
1105	24	0.30396484720019856
1105	25	0.3041609776703069
1106	1	-0.5516732022856985
1106	2	-0.43667320778868657
1106	3	-0.31484919891745733
1106	4	-1.5642781128476173
1106	5	-0.8003682703481232
1106	6	-0.5727586043456725
1106	7	-0.3550217719361939
1106	8	-0.3045138897321067
1106	9	-0.21235809331665934
1106	10	-0.13101834576687565
1106	11	-0.08055654279824864
1106	12	-0.10059901923468573
1106	13	-0.17506065737660492
1106	14	-0.11672410796094823
1106	15	-0.06723701099844703
1106	16	-0.08941057360527793
1106	17	-0.14990847033455879
1106	18	-0.22911429047562848
1106	19	-0.2597801683848928
1106	20	-0.05053223254458317
1106	21	-0.07401599122086111
1106	22	0.04683367868548771
1106	23	-0.04447329139277656
1106	24	0.05371449294025865
1106	25	-0.005226371034993349
1107	1	0.24875284679187715
1107	2	-0.025749395439714107
1107	3	0.2732377940440003
1107	4	0.5702609843122346
1107	5	0.27012652359596245
1107	6	0.218811082212099
1107	7	0.03289517716037693
1107	8	0.0424432906504606
1107	9	0.023007195440733597
1107	10	0.07134150435666697
1107	11	-0.045194818008404525
1107	12	-0.02004715435477067
1107	13	-0.0020985035160592247
1107	14	0.04743339964807802
1107	15	0.08230571162677336
1107	16	0.04340900605767928
1107	17	0.06737249165776404
1107	18	0.04151539189253839
1107	19	-0.12503459353968793
1107	20	-0.05124541344276423
1107	21	-0.10403437955018546
1107	22	0.009114215035488371
1107	23	0.005416249824847341
1107	24	0.127738680077222
1107	25	0.07207821580092855
1108	1	-0.5572236917337179
1108	2	-0.38664267915631356
1108	3	-0.20565608136202584
1108	4	-1.1187931035544025
1108	5	-0.6858253168395478
1108	6	-0.5824726290647604
1108	7	-0.6541470335677602
1108	8	-0.7349721597445935
1108	9	-0.5040974849138848
1108	10	-0.45835447907602855
1108	11	-0.36047905709047967
1108	12	-0.26907292022372076
1108	13	-0.23397963098101826
1108	14	-0.3441169825543966
1108	15	-0.3297857241631377
1108	16	-0.32697193774018984
1108	17	-0.3168339638411837
1108	18	-0.506545018989371
1108	19	-0.5683764333591697
1108	20	-0.4086848622437529
1108	21	-0.352239632657124
1108	22	-0.37634454200603074
1108	23	-0.37469657804752643
1108	24	-0.45842152934128677
1108	25	-0.4142484728145206
1109	1	-0.20720180354021522
1109	2	-0.40239225532302925
1109	3	0.031212338344751345
1109	4	-0.689092370691121
1109	5	-0.41747644613293744
1109	6	-0.1773882898668404
1109	7	-0.16377220922671398
1109	8	-0.03724124216557101
1109	9	0.0662461501826428
1109	10	0.04768118551211842
1109	11	0.0838196886988598
1109	12	-0.03507362858443486
1109	13	0.0033831336714769122
1109	14	0.05990500639731522
1109	15	0.05963395818898931
1109	16	0.09571531794978938
1109	17	-0.08960665409290414
1109	18	-0.09045360916071843
1109	19	-0.009689453990937125
1109	20	0.110806221036718
1109	21	0.08420779713081572
1109	22	0.0244549018392064
1109	23	0.11416992649002822
1109	24	0.2263913466413925
1109	25	0.12048963599976048
1110	1	0.33365953253643355
1110	2	-9.212683974944014E-4
1110	3	0.17699893523182553
1110	4	0.5950765456309028
1110	5	0.39800495709966555
1110	6	0.3394034139105102
1110	7	0.2148392413464535
1110	8	0.27019143502787557
1110	9	0.23834462409929813
1110	10	0.14687333484780396
1110	11	0.22902678357102246
1110	12	0.24913100605082406
1110	13	0.09565108546095757
1110	14	0.16025901354558414
1110	15	0.12153269032695846
1110	16	0.2104592024544374
1110	17	0.17211284207635202
1110	18	0.14022630458139673
1110	19	-0.0037889359373082243
1110	20	0.2746833150573522
1110	21	0.20946409674117916
1110	22	0.22957965127017133
1110	23	0.1802102338700422
1110	24	0.2667431904382127
1110	25	0.3504399939883953
1111	1	0.1652978328669512
1111	2	-0.31459922647436755
1111	3	0.02818121452149569
1111	4	0.4097783196501652
1111	5	0.23728420372481196
1111	6	0.0911454686556894
1111	7	0.05811597793030643
1111	8	-0.05364050094342332
1111	9	0.016115897718719907
1111	10	-0.04827458315449462
1111	11	-0.10152744170291328
1111	12	-0.08393036083430161
1111	13	-0.09008535567200227
1111	14	0.0051066553980305684
1111	15	-0.007511885559885911
1111	16	-0.003176030551244935
1111	17	-0.14087209087971786
1111	18	-0.07142753625751652
1111	19	-0.13059105723935716
1111	20	-0.02415159988894544
1111	21	0.015994553645718668
1111	22	-0.05066084149533623
1111	23	-0.10053761345238854
1111	24	0.025701659222399087
1111	25	-0.08515800975854404
1112	1	0.15173295877986348
1112	2	-0.2606821769450143
1112	3	0.10130887325680504
1112	4	0.4596960441680409
1112	5	0.17983778183933907
1112	6	0.16632762821195257
1112	7	0.0684183774712892
1112	8	0.031636050751281175
1112	9	-0.001488011883964191
1112	10	0.06810339745201333
1112	11	0.08538755998905856
1112	12	0.11987439171162606
1112	13	0.10136159604704886
1112	14	0.15811536991768094
1112	15	0.12842041172249452
1112	16	0.02701439797461802
1112	17	0.11227706015292682
1112	18	0.051516454642744236
1112	19	-0.015296155869982897
1112	20	0.12253621089527655
1112	21	0.23963114616390158
1112	22	0.2421061558160602
1112	23	0.15579522144358293
1112	24	0.17650378439171277
1112	25	0.2503575703560941
1113	1	0.17924927782476274
1113	2	-7.36665922143942E-4
1113	3	0.042201147811734785
1113	4	0.5406656284701583
1113	5	0.16357394565033445
1113	6	0.18166722321350193
1113	7	-0.014864091876468422
1113	8	-0.10309022727766859
1113	9	-0.09791717577483894
1113	10	-0.03522773279928515
1113	11	-0.06462187552163812
1113	12	-0.1301908669135301
1113	13	-0.15218347892356832
1113	14	-0.044437308757970584
1113	15	0.03978603293457894
1113	16	-0.11306370172230373
1113	17	-0.15672908653371564
1113	18	-0.1254453635757784
1113	19	-0.23655940837390688
1113	20	-0.10365018388824865
1113	21	-0.11398019681208638
1113	22	-0.23750524157868824
1113	23	-0.15056957909850155
1113	24	-0.09315170097534775
1113	25	-0.07305574977630158
1114	1	0.22481418759203464
1114	2	0.020797042642797692
1114	3	0.10391084370862558
1114	4	0.5406002021452494
1114	5	0.22157597740063295
1114	6	0.23175180764212655
1114	7	0.07735220117484687
1114	8	-0.08655193499095049
1114	9	0.02835175228077702
1114	10	0.07550395160102141
1114	11	-0.048980415230204244
1114	12	0.0020571135120164286
1114	13	0.14210731997150816
1114	14	0.08883525308778673
1114	15	-0.004848598969076079
1114	16	-0.060427318220597215
1114	17	0.02512885731256631
1114	18	-0.07534526302266897
1114	19	-0.08899528906697896
1114	20	-0.017445009864140104
1114	21	-0.046269984573051204
1114	22	-0.06081796001662809
1114	23	-0.022031590238213994
1114	24	-0.03766043676893105
1114	25	-0.18104021813569204
1115	1	0.09281049814349629
1115	2	-0.07287596121975426
1115	3	0.14139893263988412
1115	4	0.5474250411155239
1115	5	0.27856706331319714
1115	6	0.2863069266423537
1115	7	0.11006397367425791
1115	8	0.09086166849721167
1115	9	-0.15636789693324715
1115	10	-0.13491510637879145
1115	11	-0.09563672812404178
1115	12	-0.08462435333299112
1115	13	-0.025021005568106602
1115	14	0.04067199659210985
1115	15	0.06717263045305089
1115	16	-0.16401974124206475
1115	17	-0.13305182195372822
1115	18	-0.11818893777614647
1115	19	-0.3096601638490881
1115	20	0.011730183881233813
1115	21	-0.11220762061598322
1115	22	-0.011134774841067951
1115	23	-0.061141464825126945
1115	24	0.06663916709343454
1115	25	0.02021710842055313
1116	1	0.2641080314059856
1116	2	0.02137643390020827
1116	3	0.21110167459772455
1116	4	0.7068342615991483
1116	5	0.38038468263728475
1116	6	0.3478896645020271
1116	7	0.14691979237437636
1116	8	0.11128928454677833
1116	9	0.08441855017322553
1116	10	0.03535748227932345
1116	11	0.06843789877161299
1116	12	0.07270532646724348
1116	13	0.07666796331994163
1116	14	-0.05113170100302966
1116	15	0.02161366198687631
1116	16	0.08280580322208664
1116	17	0.020096437428371416
1116	18	-0.1073366587710396
1116	19	-0.06318007111791957
1116	20	-0.07019917087776406
1116	21	0.023513205527130765
1116	22	0.02480337491495277
1116	23	0.05641192691384589
1116	24	-0.023783125351265255
1116	25	0.04001778461881855
1201	1	0.5672475413306302
1201	2	0.47563538980698183
1201	3	0.6754920176956603
1201	4	0.9030406400349307
1201	5	0.6110689579742257
1201	6	0.6246663288715766
1201	7	0.5773681808195334
1201	8	0.480553041791147
1201	9	0.5541904583942454
1201	10	0.4585552860910198
1201	11	0.5140467648651352
1201	12	0.479445726616369
1201	13	0.6221116634001547
1201	14	0.544176318925345
1201	15	0.5412010858715561
1201	16	0.6163435417355956
1201	17	0.7423170278234323
1201	18	0.652547657236056
1201	19	0.7019226775066372
1201	20	0.7032450262821044
1201	21	0.6373130405870668
1201	22	0.7324776069833661
1201	23	0.692408176221889
1201	24	0.847686987734356
1201	25	0.7867109622005231
1202	1	0.7116694673992967
1202	2	0.569789344896666
1202	3	0.6800922726885794
1202	4	0.9407800073700869
1202	5	0.771770250796628
1202	6	0.6228647665208271
1202	7	0.6764908639762979
1202	8	0.5904562358474053
1202	9	0.6769729751826645
1202	10	0.6594999850207941
1202	11	0.6378036743841733
1202	12	0.7561075412190732
1202	13	0.7528479658367075
1202	14	0.7932124124670494
1202	15	0.8141378858350095
1202	16	0.8232598093701853
1202	17	0.8188695152502916
1202	18	0.8460399389243278
1202	19	0.8352545919054322
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2210	9	0.058523686858613644
2210	10	-0.17757284633428583
2210	11	-0.10603444026202169
2210	12	-0.058993754030367995
2210	13	-0.015976316887773123
2210	14	-0.015698213671036854
2210	15	-0.06088569130428567
2210	16	-0.07431549797747294
2210	17	-0.10522526013423317
2210	18	-0.009976043232498455
2210	19	-0.0403851344740076
2210	20	0.07306370504178972
2210	21	-0.0461545892277897
2210	22	-0.11253705903202871
2210	23	-0.07696462010990501
2210	24	-0.08426615806297377
2210	25	-0.2046648577159189
2211	1	0.21024440030658198
2211	2	0.1572445164349574
2211	3	0.2965560937106808
2211	4	0.6985584663314839
2211	5	0.27024581217320787
2211	6	0.16155173865190164
2211	7	0.08793680005805271
2211	8	0.058749460624838434
2211	9	-0.11969555312786895
2211	10	-0.026206247577093222
2211	11	-0.10021329947308288
2211	12	0.010762908752468547
2211	13	0.010343598133786713
2211	14	0.05658528541314922
2211	15	-0.012322362843455892
2211	16	-0.10100428010019158
2211	17	-0.041035204191075536
2211	18	-0.04505505600162962
2211	19	-0.02774883568667974
2211	20	0.023286530412811146
2211	21	-0.0440991700527249
2211	22	-0.17604479829094544
2211	23	-0.07047773265874469
2211	24	-3.4353579522106443
2211	25	-2.5777442425741057
2212	1	0.16929744478217756
2212	2	0.005162072155219022
2212	3	-0.2962172769177087
2212	4	0.4437661592951443
2212	5	0.21897378304566928
2212	6	0.12234563909141372
2212	7	0.13896286149446269
2212	8	0.20330487356660853
2212	9	0.07579242796311547
2212	10	-0.025695412672909868
2212	11	0.09758571881088329
2212	12	0.0602474265745343
2212	13	0.017856477655818992
2212	14	0.014480346137219158
2212	15	0.07624910537914076
2212	16	0.07333093606377616
2212	17	0.07308723074663703
2212	18	0.002065012935062782
2212	19	0.12587962600727565
2212	20	-0.25101373533090054
2212	21	-0.13925159789260988
2212	22	-0.10123656452704921
2212	23	0.05494962935694048
2212	24	0.011717177862806238
2212	25	0.17764618411501232
2213	1	-0.23326226297029962
2213	2	-0.5827347842229891
2213	3	-0.26886482596757943
2213	4	0.23400230313844617
2213	5	-0.13265957849080934
2213	6	-0.10792583736138539
2213	7	-0.2741094777566886
2213	8	-0.3609476402051186
2213	9	-0.4185587120854777
2213	10	-0.4259590455042712
2213	11	-0.46729846067731984
2213	12	-0.5565346551080665
2213	13	-0.46223869888012814
2213	14	-0.43917910270312177
2213	15	-0.4965070197266215
2213	16	-0.5841101550711159
2213	17	-0.5503444988808326
2213	18	-0.6356126941129432
2213	19	-0.6275492348882779
2213	20	-6.202836832617223
2213	21	-3.156160593727506
2213	22	-1.875870801208329
2213	23	-1.1882013639868703
2213	24	-0.7937232451117424
2213	25	-0.6407652646672233
2214	1	-0.20268646442076843
2214	2	-0.38521955076029357
2214	3	-0.15527324080944993
2214	4	0.16792156139612757
2214	5	-0.1654488386801063
2214	6	-0.2529088917804003
2214	7	-0.25578717807452733
2214	8	-0.31339377432983184
2214	9	-0.4492381157415082
2214	10	-0.6234514507231594
2214	11	-0.6610364977995573
2214	12	-0.5313867105280181
2214	13	-0.6193031623285989
2214	14	-0.6363290842649292
2214	15	-0.6196495908892814
2214	16	-0.6636999490901161
2214	17	-0.6543903660906878
2214	18	-0.4840296432059574
2214	19	-0.5704663332428908
2214	20	-0.4241039757503131
2214	21	-0.5147949379422982
2214	22	-0.526674179788408
2214	23	-0.5676304822898572
2214	24	-0.40806465027795014
2214	25	-0.43075595776782905
2215	1	-0.18268721156760392
2215	2	-0.1764891400163222
2215	3	-0.1306416775914876
2215	4	0.1764614496976762
2215	5	-0.22133728965793864
2215	6	-0.29889577935348655
2215	7	-0.35666133986917714
2215	8	-0.5166756167939255
2215	9	-0.6371885051917943
2215	10	-0.5943071206336867
2215	11	-0.611056110403922
2215	12	-0.5475512322606519
2215	13	-0.5701352971138931
2215	14	-0.6973149445111133
2215	15	-0.6794437519367804
2215	16	-0.832334725039459
2215	17	-0.6943795324616104
2215	18	-0.6898835386427606
2215	19	-0.6859229333512289
2215	20	-0.572476129587848
2215	21	-0.6158988473881024
2215	22	-0.6650233588608288
2215	23	-0.654837460641275
2215	24	-0.6905926215875127
2215	25	-0.7747423596954661
2216	1	-0.9038630303474626
2216	2	-0.48214011863188944
2216	3	-2.248688253378731
2216	4	-0.4792509830353211
2216	5	-0.6263955952310738
2216	6	-0.5530716424696465
2216	7	-0.4062741639283871
2216	8	-0.39326418156109355
2216	9	-0.5773819981558219
2216	10	-0.47691792222406093
2216	11	-0.541443779711102
2216	12	-0.6393658838423093
2216	13	-0.5957983498717567
2216	14	-0.643874030636546
2216	15	-0.8021603072793226
2216	16	-0.6835992597632838
2216	17	-0.6138806390357558
2216	18	-0.7986344916232753
2216	19	-0.6402992098950904
2216	20	-0.6348288072172465
2216	21	-0.6913377351269148
2216	22	-0.8842847160384224
2216	23	-0.8314711284303513
2216	24	-0.7667192220697459
2216	25	-0.8694450381082532
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47547.0
3	0.0
4	804.0
5	6177.0
6	7059.0
7	5603.0
8	4377.0
9	3943.0
10	3617.0
11	3260.0
12	3089.0
13	2832.0
14	2782.0
15	2893.0
16	2958.0
17	3172.0
18	3516.0
19	4177.0
20	5393.0
21	7021.0
22	8918.0
23	11138.0
24	12664.0
25	14394.0
26	16262.0
27	17457.0
28	19921.0
29	23942.0
30	30403.0
31	38559.0
32	49402.0
33	64586.0
34	85099.0
35	116438.0
36	170804.0
37	281992.0
38	1004702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57570758295386	18.88550763327258	13.154407401076668	27.38437738269689
2	13.799477267463855	20.152293439855313	39.26425227718538	26.78397701549546
3	17.198180459925986	24.21264832399812	29.2990898945374	29.29008132153849
4	10.344333535706774	16.914362492518812	37.46219873391215	35.27910523786227
5	12.88227030711031	37.77192732724356	35.769817522498755	13.575984843147376
6	33.002994507503594	36.61916061414347	17.511951087144787	12.865893791208158
7	28.488542873120327	30.96900760805552	21.72840914155047	18.814040377273685
8	25.87947346396231	36.595222904442274	19.83483553298568	17.690468098609742
9	26.12580784640444	15.0135995267729	19.509017296373813	39.351575330448846
10	14.008623581345155	29.662513010126336	34.48617907684169	21.842684331686822
11	34.78781739218357	22.728603596588286	23.064474933283368	19.41910407794477
12	22.792080545982838	25.188590948497097	31.355818080481768	20.663510425038304
13	28.813731518093377	21.166069153108996	25.514364047015796	24.505835281781835
14	20.990513485111823	21.575172616044128	27.509263931357058	29.925049967486988
15	24.223932255466124	29.29854295775694	22.962826662909897	23.51469812386704
16	22.560982038706065	28.19469778078959	26.926557008106016	22.317763172398326
17	22.44148665664263	28.169469769483957	27.181797222764114	22.207246351109298
18	22.361245232754726	27.65635173999058	28.23350367714398	21.74889935011071
19	23.23340871377449	27.43538100355365	27.56527565946702	21.765934623204842
20	23.413664567701105	27.188544161893642	27.64390835981199	21.753882910593266
21	22.984894827306135	26.754311776169544	27.491002208537925	22.769791187986396
22	22.74875521167511	27.31006406149597	26.859587493608945	23.081593233219973
23	22.189440978907452	27.207223352554845	27.196825144305087	23.40651052423262
24	22.50352796858491	27.66373008704319	27.42787730096627	22.404864643405634
25	22.800314916711432	26.692162206065355	26.944306414151892	23.56321646307132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	102.0
2	274.0
3	446.0
4	446.0
5	446.0
6	1020.5
7	1595.0
8	1595.0
9	1595.0
10	2371.5
11	3148.0
12	3148.0
13	3148.0
14	5536.5
15	7925.0
16	7925.0
17	7925.0
18	14630.5
19	21336.0
20	21336.0
21	21336.0
22	33731.5
23	46127.0
24	46127.0
25	46127.0
26	65757.0
27	85387.0
28	85387.0
29	85387.0
30	109553.0
31	133719.0
32	133719.0
33	133719.0
34	156789.5
35	179860.0
36	179860.0
37	179860.0
38	201046.5
39	222233.0
40	222233.0
41	222233.0
42	238824.0
43	255415.0
44	255415.0
45	255415.0
46	280286.5
47	305158.0
48	305158.0
49	305158.0
50	291865.5
51	278573.0
52	278573.0
53	278573.0
54	245071.5
55	211570.0
56	211570.0
57	211570.0
58	180257.0
59	148944.0
60	148944.0
61	148944.0
62	121531.5
63	94119.0
64	94119.0
65	94119.0
66	72878.0
67	51637.0
68	51637.0
69	51637.0
70	37892.5
71	24148.0
72	24148.0
73	24148.0
74	17014.0
75	9880.0
76	9880.0
77	9880.0
78	6814.5
79	3749.0
80	3749.0
81	3749.0
82	2530.0
83	1311.0
84	1311.0
85	1311.0
86	864.0
87	417.0
88	417.0
89	417.0
90	249.5
91	82.0
92	82.0
93	82.0
94	50.5
95	19.0
96	19.0
97	19.0
98	10.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20015324157686445
2	0.06334751864127718
3	0.0
4	0.0
5	0.021658909550572834
6	0.4039003287649965
7	0.0016292100104413195
8	0.035363440814873345
9	0.03828643524537101
10	0.003114666196431934
11	0.00431261473352114
12	0.01159614183902351
13	0.009487752413746508
14	0.0021083894252770016
15	9.583588296713642E-5
16	0.01451913626952117
17	0.027888241943436705
18	0.0630120930508922
19	0.02851117518272309
20	0.0
21	0.0
22	0.0
23	1.4375382445070466E-4
24	2.8750764890140933E-4
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2086901.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.75190055066405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.32087413678329	62.18824723681101
2	11.13760090341671	16.428384684035503
3	2.4875173916035256	5.503774058507713
4	0.8664137911184283	2.5559865503316055
5	0.4006729263183861	1.4775194907588578
6	0.20777953469748983	0.9194481347683512
7	0.11802810282231636	0.609335783107452
8	0.09228199593901584	0.5444778069688864
9	0.0560578847438709	0.3720937986639498
>10	0.2838581358518375	3.572422880002363
>50	0.016614377933623126	0.8334473192511791
>100	0.009179043635649245	1.2914066028023405
>500	0.001821035495913957	1.0081016381288497
>1k	0.001170665675944554	1.753541403944978
>5k	6.503698199691966E-5	0.45399828733353076
>10k+	6.503698199691966E-5	0.4878143245834134
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	10170	0.48732546488788875	No Hit
CCCATGTACTCTGCGTTGATACCAC	9465	0.4535433161419732	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3915	0.18759874090816958	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3402	0.16301683692709906	No Hit
CATGTACTCTGCGTTGATACCACTG	2678	0.12832424729299569	No Hit
GTATCAACGCAGAGTACATGGGAAG	2579	0.12358037108612244	No Hit
GCGTTGATACCACTGCTTCCCATGT	2490	0.11931567429408488	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2374	0.11375719308199095	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2320	0.11116962424187826	No Hit
TATCAACGCAGAGTACATGGGAAGC	2300	0.1102112654122069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.791794148356822E-5
2	0.0	0.0	0.0	0.0	4.791794148356822E-5
3	0.0	0.0	0.0	0.0	4.791794148356822E-5
4	0.0	0.0	0.0	0.0	4.791794148356822E-5
5	0.0	0.0	0.0	0.0	4.791794148356822E-5
6	0.0	0.0	0.0	0.0	4.791794148356822E-5
7	0.0	0.0	0.0	0.0	4.791794148356822E-5
8	0.0	0.0	0.0	0.0	4.791794148356822E-5
9	0.0	0.0	0.0	0.0	4.791794148356822E-5
10	0.0	0.0	0.0	4.791794148356822E-5	4.791794148356822E-5
11	0.0	0.0	0.0	4.791794148356822E-5	4.791794148356822E-5
12	0.0	0.0	0.0	9.583588296713644E-5	4.791794148356822E-5
13	0.0	0.0	0.0	9.583588296713644E-5	4.791794148356822E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCGCC	130	1.8189894E-12	13.865383	11
TAGGACC	410	0.0	12.308317	4
CTACGCT	55	0.0030174516	12.118309	4
GTAGGAC	765	0.0	11.824121	3
TGGCGAG	660	0.0	11.788377	18
GACCGTG	105	2.7690476E-7	11.746177	7
GGACGTG	620	0.0	11.51798	6
AGGACGT	640	0.0	11.455589	5
GGCGAGG	325	0.0	11.383119	19
ACGACAG	85	5.2032614E-5	11.201799	5
TAGGACG	640	0.0	11.158042	4
TGTGCGC	180	3.6379788E-12	11.06639	10
GGCGAGA	410	0.0	10.874118	19
TTAGACT	205	0.0	10.682691	4
CCAACGA	80	3.82384E-4	10.671674	19
ATGGCGA	740	0.0	10.645495	17
GGCGTGC	125	2.2826498E-7	10.625772	8
TATGGCG	735	0.0	10.589289	16
TAGGACA	505	0.0	10.558527	4
TGTAGGA	955	0.0	10.474492	2
>>END_MODULE