FastQCFastQC Report
Thu 26 May 2016
SRR1513104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000650
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC138530.6924249618873866No Hit
CCCATGTACTCTGCGTTGATACCAC113960.5696148751655712No Hit
GAGTACATGGGAAGCAGTGGTATCA57500.2874065928573214No Hit
GCGTTGATACCACTGCTTCCCATGT39580.1978357033963962No Hit
GTATCAACGCAGAGTACATGGGAAG37860.18923849748831628No Hit
CATGTACTCTGCGTTGATACCACTG36700.18344038187589035No Hit
TATCAACGCAGAGTACATGGGAAGC29150.14570264663984206No Hit
ACGCAGAGTACATGGGAAGCAGTGG27840.13915477469822307No Hit
GTATCAACGCAGAGTACTTTTTTTT27100.13545597680753754No Hit
GCTTCCCATGTACTCTGCGTTGATA24820.12405968060380376No Hit
ACTCTGCGTTGATACCACTGCTTCC24470.12231024916902007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC2050.011.1224783
GTATAAG2650.010.7662651
CTGTTCG909.524779E-510.5547369
CTTATAC1909.094947E-1210.5004983
TAGGCCC1451.439912E-810.4845674
GTATTAC1553.59978E-910.4305421
TGCGTTT2400.010.29009612
TTGGACT3100.010.1146474
GCGTTTA2450.010.08009313
CTAGACC1803.929017E-1010.0295084
TCGAACT1803.947207E-1010.02699919
CTCGAAC1856.493792E-109.75599918
GTCTAGA2450.09.7042861
GTCCTAG2950.09.6713911
GTCCTAA1804.105459E-99.51020051
GCCTAGG1804.105459E-99.51020051
TCCTAGG2600.09.50142
TCTAGAC1901.0513759E-99.500453
TCTATAC2500.09.500453
CGTTTAT2600.09.49854914
Produced by FastQC (version 0.11.5)