FastQCFastQC Report
Thu 26 May 2016
SRR1513104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000650
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC163700.818234073925974No Hit
CCCATGTACTCTGCGTTGATACCAC150070.7501062154799689No Hit
GAGTACATGGGAAGCAGTGGTATCA62410.31194861669957263No Hit
CATGTACTCTGCGTTGATACCACTG42940.21463024517031967No Hit
GTATCAACGCAGAGTACATGGGAAG42670.21328068377777223No Hit
GTATCAACGCAGAGTACTTTTTTTT41540.20763251943118488No Hit
GCGTTGATACCACTGCTTCCCATGT39480.1973358658436008No Hit
ACGCAGAGTACATGGGAAGCAGTGG36020.180041486516882No Hit
TATCAACGCAGAGTACATGGGAAGC34410.171994101916877No Hit
ACTCTGCGTTGATACCACTGCTTCC29990.14990128208332293No Hit
GCTTCCCATGTACTCTGCGTTGATA29380.14685227301127135No Hit
TATCAACGCAGAGTACTTTTTTTTT28620.14305350761002675No Hit
GGTATCAACGCAGAGTACTTTTTTT24650.12320995676405168No Hit
GGTATCAACGCAGAGTACATGGGAA24000.11996101267088197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC854.009891E-612.2761628
AGACCCG701.07182466E-412.2377485
TCGAACT1501.4551915E-1112.01666119
AAGGCGT1750.011.9657986
CCGGCCA752.102729E-411.383359
GTATTAG1451.1568773E-911.1727781
TAGGCCG600.005793300511.1046235
GGACCGG600.005793300511.1046236
AGAACGC600.00594093211.0671468
CTCGAAC1900.010.98997918
AGGCGTG2000.010.9090447
CGTGCGC1752.1827873E-1110.839931510
TTAGTTG909.333878E-510.5758313
TAGGACT1909.094947E-1210.5201694
GTGCGCC1951.6370905E-1110.21557111
ATAAGAC2053.6379788E-1210.2147053
GTACTAG856.4079737E-410.0902951
GGACCAT1803.8016879E-1010.047046
TGCAATA1252.4784877E-69.90817452
CACCGTC1151.044596E-59.8985657
Produced by FastQC (version 0.11.5)