FastQCFastQC Report
Thu 26 May 2016
SRR1513103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671584
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38900.23271340237762508No Hit
CCCATGTACTCTGCGTTGATACCAC32450.19412724697053813No Hit
GTCCTACAGTGGACATTTCTAAATT23620.14130309933571988No Hit
GTATCAACGCAGAGTACTTTTTTTT21880.1308938109003197No Hit
GTCCTACAGTGTGCATTTCTCATTT18430.11025470451978482No Hit
CTGTAGGACGTGGAATATGGCAAGA18320.1095966460554779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6550.013.2026114
GACGTAT550.003041565712.1052091
GTACTAG1209.840733E-911.8890441
GGCGAGG4750.011.79946119
GTCCTAC15050.011.5019591
GTAGGAC14950.011.4413713
TGTAGGA16250.011.3444162
AGGACGT12250.011.2474145
GGACGTG11650.011.089296
TTAGGAC7200.011.0864763
TGGCGAG11700.011.04223118
TCTAGAC1900.011.0031193
TGGCGGG951.3609426E-510.99949718
GTCCTAA5750.010.9172441
TAGACAG2700.010.9098475
GTATTAG2800.010.8699841
GACCGTG700.001493935410.8550237
CCGTGCA700.001493935410.8550239
GTGCTAG2550.010.8166991
TAGGACG12550.010.7523754
Produced by FastQC (version 0.11.5)