##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513103_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671584
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.379739815647913	31.0	30.0	34.0	25.0	34.0
2	29.092580450638437	31.0	30.0	34.0	16.0	34.0
3	29.456878027068935	31.0	30.0	34.0	23.0	34.0
4	32.337138905373585	35.0	33.0	37.0	19.0	37.0
5	32.560229100063175	35.0	33.0	37.0	23.0	37.0
6	33.012154938070715	37.0	35.0	37.0	27.0	37.0
7	33.024615574209854	37.0	35.0	37.0	27.0	37.0
8	33.00568801807148	37.0	35.0	37.0	26.0	37.0
9	34.3834698106706	39.0	35.0	39.0	27.0	39.0
10	33.89788488044872	39.0	34.0	39.0	22.0	39.0
11	34.25733615540709	39.0	35.0	39.0	26.0	39.0
12	34.11675273273733	39.0	35.0	39.0	25.0	39.0
13	34.19380838773283	39.0	35.0	39.0	25.0	39.0
14	34.54231256101997	40.0	34.0	41.0	17.0	41.0
15	35.06996357945518	40.0	34.0	41.0	25.0	41.0
16	34.66687285831882	40.0	34.0	41.0	18.0	41.0
17	34.216352274250056	39.0	33.0	41.0	17.0	41.0
18	33.636300060302084	39.0	32.0	41.0	11.0	41.0
19	34.52939367689569	39.0	34.0	41.0	18.0	41.0
20	34.784627634626794	40.0	34.0	41.0	19.0	41.0
21	34.81196218676417	40.0	35.0	41.0	18.0	41.0
22	34.39834911078354	40.0	34.0	41.0	15.0	41.0
23	32.73591336121906	39.0	31.0	41.0	8.0	41.0
24	33.82604523613531	39.0	33.0	41.0	2.0	41.0
25	33.55864198269426	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7283585740494445
1101	2	0.9714792885413495
1101	3	0.8820815053758579
1101	4	0.8418306802978606
1101	5	0.9992042945361916
1101	6	0.6102410105596121
1101	7	0.7435476699363548
1101	8	0.6882710354779462
1101	9	0.750347093873863
1101	10	1.0711349787167421
1101	11	0.8875348279819448
1101	12	0.8315719732513571
1101	13	0.8183855007795202
1101	14	-0.01235022784535289
1101	15	0.5977453995659303
1101	16	1.2355103759506534
1101	17	1.7730260223400407
1101	18	-4.4090410543748035
1101	19	-0.8551692262193598
1101	20	0.11593903391668903
1101	21	0.6141520298143135
1101	22	1.1099114817971767
1101	23	2.869449984671114
1101	24	1.8105754161690086
1101	25	2.11809280372497
1102	1	1.078285650591912
1102	2	1.3512940874361838
1102	3	1.1953976033410463
1102	4	1.6300637058183796
1102	5	1.4863280656132503
1102	6	1.0342486784178888
1102	7	1.0327378344932256
1102	8	0.9250024288025358
1102	9	1.1137005341417066
1102	10	1.3965052376472542
1102	11	1.0085326943066448
1102	12	1.120634529220034
1102	13	0.9827650633462355
1102	14	1.8836813092366569
1102	15	1.2560718375580038
1102	16	1.6919389831991793
1102	17	1.0469747962229974
1102	18	1.6260563818716491
1102	19	1.4475671496609124
1102	20	1.403956542710489
1102	21	1.386983071864556
1102	22	1.8329220415197867
1102	23	3.285473384653443
1102	24	2.280636799631175
1102	25	2.3911580187282766
1103	1	0.8351891510888549
1103	2	1.1779583034822565
1103	3	0.8918768617054447
1103	4	1.27476001737368
1103	5	1.1549054442593985
1103	6	0.8740902945279743
1103	7	0.7760788947014845
1103	8	0.7201408748283171
1103	9	0.8267511115817143
1103	10	1.2439838859635444
1103	11	0.9703524966847539
1103	12	1.003094683573039
1103	13	0.8928347074968741
1103	14	1.722381190836785
1103	15	1.2185432001912986
1103	16	1.404827723419757
1103	17	1.824770940285795
1103	18	1.8103036944112034
1103	19	1.3464112167926814
1103	20	1.3050697902600632
1103	21	1.2609750485888753
1103	22	1.4720115056159884
1103	23	2.965668900860024
1103	24	1.8723741372224083
1103	25	1.9602984585404712
1104	1	0.683303364573483
1104	2	1.136265241328033
1104	3	0.8993985751745086
1104	4	1.493378537090031
1104	5	1.2973251691218266
1104	6	0.8751810445115993
1104	7	0.6974971690843219
1104	8	0.7470826513975481
1104	9	0.9614874151027948
1104	10	1.2436369032605015
1104	11	0.8341916520449573
1104	12	0.9552119598729476
1104	13	0.7817559247851165
1104	14	1.652006250902886
1104	15	1.0004686473780424
1104	16	1.3883966621092014
1104	17	1.8539901150746658
1104	18	2.4347486480732243
1104	19	1.605319897644776
1104	20	0.9615988604738916
1104	21	1.1320775701941415
1104	22	1.4065728800528632
1104	23	2.9862550096350304
1104	24	1.931548398427907
1104	25	2.0562808998224327
1105	1	0.6363511347210107
1105	2	1.0762826558163248
1105	3	0.7722085624539545
1105	4	0.8937721233011402
1105	5	0.9677040515904594
1105	6	0.7864111598415064
1105	7	0.4611139728920364
1105	8	0.6119713347965146
1105	9	0.8354239460991764
1105	10	1.302330124283685
1105	11	0.7666324732953314
1105	12	0.8645876786313096
1105	13	0.8032767235984934
1105	14	1.660974301653681
1105	15	0.952807633770334
1105	16	1.3963030040809343
1105	17	1.9516327049986728
1105	18	2.0270037773675895
1105	19	1.4209052779236941
1105	20	1.1283235167607089
1105	21	0.976764156344224
1105	22	1.5355046349732788
1105	23	2.953165721657598
1105	24	1.9602204028777521
1105	25	1.96504200683939
1106	1	-0.3433452963021324
1106	2	0.2016286059416963
1106	3	-0.08664213040977842
1106	4	-2.3549283808406294
1106	5	-0.2571621750059663
1106	6	-0.2864662901116404
1106	7	-0.11585541368329189
1106	8	-0.08892121050518398
1106	9	-0.1237649288192273
1106	10	-0.029245005605680774
1106	11	-0.1316518669967479
1106	12	-0.11849072348257295
1106	13	-0.06849546237273074
1106	14	0.6262924252250173
1106	15	0.1762902104092703
1106	16	-0.9791257452476358
1106	17	-5.722657209034679
1106	18	-6.760887245633128
1106	19	-2.659820332140839
1106	20	-1.3466854255532326
1106	21	-1.0189396188177042
1106	22	-1.2551193639090812
1106	23	-4.698268601412249
1106	24	-1.9073449813948535
1106	25	-0.5808555485668236
1107	1	0.38864195346977226
1107	2	0.6308094427240896
1107	3	0.38312976428107604
1107	4	0.7766949222483746
1107	5	0.690719232297802
1107	6	0.271159584615539
1107	7	0.24591903435696594
1107	8	0.2913961410909707
1107	9	0.2151268656255283
1107	10	0.6190781641817296
1107	11	0.397510474521404
1107	12	0.2785722167570768
1107	13	0.34073040020125944
1107	14	1.1384414834856926
1107	15	0.5483271702598032
1107	16	-6.037207364524246
1107	17	-1.2974805822110014
1107	18	0.3856085212522089
1107	19	0.4733238893280074
1107	20	0.2870897587578227
1107	21	0.2614761677015238
1107	22	0.35697403157070084
1107	23	2.1670870548495813
1107	24	1.1827832966842422
1107	25	0.994150269919416
1108	1	0.2691937638868609
1108	2	0.5074019842379478
1108	3	0.27560112111901347
1108	4	0.8232129726722306
1108	5	0.6612981678093774
1108	6	0.32502668308809746
1108	7	0.1561998111044005
1108	8	0.2749355428065243
1108	9	0.2939854732116345
1108	10	0.6932178972991636
1108	11	0.22959504718109258
1108	12	0.43522678462527864
1108	13	0.1697716904958071
1108	14	0.7919452749380866
1108	15	0.4035761390602275
1108	16	0.6648631538600966
1108	17	0.8172621696595641
1108	18	0.6884003933297151
1108	19	0.5526402042947183
1108	20	0.47901066600761055
1108	21	0.5286277583275876
1108	22	0.7744346104147297
1108	23	2.3820031122879968
1108	24	1.145343919326379
1108	25	1.3822140847917908
1109	1	0.34346975104450195
1109	2	0.6983612830218355
1109	3	0.4375698766423106
1109	4	0.45807575471420847
1109	5	0.4634681042668163
1109	6	0.2874356720526592
1109	7	0.24077079156115389
1109	8	0.1381890489728832
1109	9	0.29052134824745224
1109	10	0.7842619652586436
1109	11	0.45263922153103664
1109	12	0.5154876643371296
1109	13	0.38604419765571407
1109	14	-0.7229948632665568
1109	15	0.04124883051586892
1109	16	-0.09783205977024068
1109	17	0.8253894572690257
1109	18	1.6396675552268292
1109	19	0.7514307849635742
1109	20	0.6016814441508913
1109	21	0.507293467480082
1109	22	0.3899789434923804
1109	23	-4.290648875926685
1109	24	-1.2752973749307657
1109	25	0.044737529303155554
1110	1	0.33990114635510693
1110	2	0.6609425831550659
1110	3	0.5420238164803877
1110	4	0.9178310862770687
1110	5	0.7052574417196809
1110	6	0.17265764369545877
1110	7	0.2176424568427464
1110	8	0.1820022515401689
1110	9	0.2682155932796988
1110	10	0.590706367461614
1110	11	0.19071453167775587
1110	12	0.3557663564452014
1110	13	0.1088190164136904
1110	14	1.0435417909238183
1110	15	0.5154077042660319
1110	16	0.8799245596400738
1110	17	1.2436545379330681
1110	18	1.8042900498265766
1110	19	1.0715054705197957
1110	20	0.6835867640785338
1110	21	0.5490766803899376
1110	22	0.7254208694889925
1110	23	2.089368433668824
1110	24	1.280024253187264
1110	25	1.655955490640352
1111	1	0.5403106951829031
1111	2	0.8565441943844299
1111	3	0.5597672106364371
1111	4	1.0524841491802235
1111	5	0.7458279043142539
1111	6	0.2958315241529377
1111	7	0.2197627037592227
1111	8	0.08913029215189994
1111	9	0.3457718002453021
1111	10	0.5550098440192599
1111	11	0.29231212260690853
1111	12	0.5710675235137899
1111	13	0.3606401010411062
1111	14	-0.32351207072533583
1111	15	0.07648776104150556
1111	16	0.4954943403128098
1111	17	1.0430485390843458
1111	18	1.6514178727886417
1111	19	0.9099863832802981
1111	20	0.5594429293091139
1111	21	0.5021984761523015
1111	22	0.7879602389918361
1111	23	0.7071034045002307
1111	24	0.744784299379873
1111	25	0.2391873365737922
1112	1	0.2489723049988477
1112	2	0.6310270629855275
1112	3	0.3963895625836962
1112	4	0.9901245040770021
1112	5	0.7522700164116927
1112	6	0.14213744012249663
1112	7	0.06095202638304187
1112	8	0.06308156036321577
1112	9	0.11382250595683274
1112	10	0.560074132957908
1112	11	0.22370622266978302
1112	12	0.22647557179757882
1112	13	0.1077697545698939
1112	14	0.5330979555746893
1112	15	0.2746502560526878
1112	16	0.6653936941259744
1112	17	0.9760845427092733
1112	18	0.9814224380537411
1112	19	0.4709048145574286
1112	20	0.4694155320332598
1112	21	0.39806139653475725
1112	22	0.3534109608495086
1112	23	1.934649605778965
1112	24	0.8480637572788083
1112	25	0.8871177023521497
1113	1	0.5010993534715134
1113	2	0.8170882228942027
1113	3	0.4772735502733916
1113	4	0.7115787743687392
1113	5	0.7163930370631633
1113	6	0.3362847134714997
1113	7	0.3208133605409742
1113	8	0.19586423294502708
1113	9	0.4245513057247976
1113	10	0.7993876823046406
1113	11	0.5075351525604148
1113	12	0.5522068487489449
1113	13	0.3087168681151766
1113	14	1.0260887679892718
1113	15	0.5053805624447207
1113	16	0.981782067953823
1113	17	1.3826248993559673
1113	18	1.98973017194443
1113	19	1.1361416931032124
1113	20	0.7772178415130284
1113	21	0.7531468734687436
1113	22	0.8958641849685804
1113	23	0.734711140368681
1113	24	0.7447145658337249
1113	25	1.1730107636429778
1114	1	-0.3487547267175657
1114	2	-3.093644311016355
1114	3	-0.9480286464328174
1114	4	0.07861041027474869
1114	5	0.24689698698313833
1114	6	0.03890764334489916
1114	7	-0.041910844181415996
1114	8	-0.009221799902483951
1114	9	0.05642802615159326
1114	10	0.25168999590292884
1114	11	0.1323269298305192
1114	12	0.1613533608316473
1114	13	-0.05108447672955663
1114	14	0.849985135212286
1114	15	0.21089901228281605
1114	16	0.5629259531730568
1114	17	0.8812666915374763
1114	18	0.990232333610912
1114	19	0.38569163070962986
1114	20	0.22246854990970633
1114	21	0.1166227863079854
1114	22	0.42970848639614445
1114	23	2.067970172373954
1114	24	0.9173772752804723
1114	25	0.9714455815242786
1115	1	-0.16352575476274467
1115	2	0.1810213828672893
1115	3	-0.07359523044629412
1115	4	-0.022152987132876945
1115	5	0.042977624170845274
1115	6	-0.3421896514545111
1115	7	-0.4913157467631635
1115	8	-0.5505927900224705
1115	9	-0.4903806690605492
1115	10	-0.3129018328309101
1115	11	-0.5156474923524925
1115	12	-0.5532848734337037
1115	13	-0.7575273662287856
1115	14	0.04304716069556491
1115	15	-0.5711623720311678
1115	16	-0.17468482647879569
1115	17	0.4326116734284753
1115	18	0.8616970745824091
1115	19	0.07905032906366216
1115	20	-0.411749926999029
1115	21	-0.2932058997064644
1115	22	-0.025314015698917558
1115	23	1.4648709279305265
1115	24	0.25756513287971927
1115	25	0.03000107658983353
1116	1	-0.11490030125288442
1116	2	-0.21826308759041524
1116	3	-0.08559488632760903
1116	4	0.5557658661212699
1116	5	-0.1282920254510671
1116	6	-0.2789513166329627
1116	7	-0.21277421668096252
1116	8	-0.3277189836841643
1116	9	-0.3625794391329009
1116	10	0.24267274154470186
1116	11	-0.24709727030694495
1116	12	-0.19008718021551374
1116	13	-0.3173722754971493
1116	14	0.3404805099493302
1116	15	-0.2771669464364521
1116	16	0.2114015057796621
1116	17	0.5325953574563229
1116	18	1.0130051705677943
1116	19	0.1364522923200937
1116	20	-0.03859622757482839
1116	21	-0.1288764713291286
1116	22	0.3508237267045189
1116	23	1.601203631368449
1116	24	0.6322632132314325
1116	25	0.9420108187084821
1201	1	1.0924592320647655
1201	2	1.4041070330221679
1201	3	1.1456794687355192
1201	4	1.6813533090753054
1201	5	1.5240780795805051
1201	6	1.0746872382597132
1201	7	0.913715366636147
1201	8	0.8886477745468966
1201	9	1.1915036419179472
1201	10	1.4103100530729407
1201	11	1.0190386552906219
1201	12	1.163343679840544
1201	13	1.0286074349142353
1201	14	1.9219289827768335
1201	15	1.3378369410250457
1201	16	1.5948446592510308
1201	17	2.1012301651165686
1201	18	2.626658340445964
1201	19	1.733181994551451
1201	20	1.50355039905903
1201	21	1.3872417698243638
1201	22	1.7182323090353435
1201	23	3.191448518071148
1201	24	2.0537140543721506
1201	25	2.164401817871777
1202	1	0.9921847413313643
1202	2	1.2581169948888729
1202	3	1.0646158198138806
1202	4	1.5746992624617775
1202	5	1.3849018630604348
1202	6	1.0303309456561607
1202	7	0.9766065357676936
1202	8	0.8326668627078746
1202	9	1.0621214451955723
1202	10	1.4016982858753835
1202	11	1.0697937298749238
1202	12	1.169234953800121
1202	13	1.1099155299723478
1202	14	1.943385005894541
1202	15	1.4358766264433172
1202	16	1.7544785149493833
1202	17	2.0016845877376994
1202	18	2.6868406130351445
1202	19	1.9622831087344963
1202	20	1.6404721263990538
1202	21	1.6465185541094982
1202	22	2.008078593309108
1202	23	3.5242872625091195
1202	24	2.236894084984371
1202	25	2.536349655324557
1203	1	0.718435107113649
1203	2	1.063253035417553
1203	3	0.768381396042912
1203	4	1.2816747306630987
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2211	15	-0.2969426606073995
2211	16	-0.49644671299571996
2211	17	0.14041530990939322
2211	18	-5.256593655933521
2211	19	-1.7401485485286088
2211	20	-0.9664784254450396
2211	21	-0.9110097753051605
2211	22	-0.49971645759190864
2211	23	-4.871163509478173
2211	24	-2.1927795581773317
2211	25	-1.5012952698573443
2212	1	0.11483947458352972
2212	2	0.3734798802970687
2212	3	0.08162028911520025
2212	4	0.6369834656028601
2212	5	0.5738926095030905
2212	6	0.1551445289005713
2212	7	-0.07154965708800631
2212	8	-0.1254353471459808
2212	9	-0.20665464433618297
2212	10	-3.6142333689530624
2212	11	-1.108820257874747
2212	12	-0.27316270942397836
2212	13	-0.20539908392608197
2212	14	0.6133312755615847
2212	15	0.18650007223585163
2212	16	0.4891729501227715
2212	17	1.0100280963801467
2212	18	1.496416705152349
2212	19	0.670708940426465
2212	20	0.28135589165907504
2212	21	0.09495470996976962
2212	22	0.35884015496736765
2212	23	1.5898235492871322
2212	24	0.7461432353117843
2212	25	0.6491488329167083
2213	1	-1.495265901257735
2213	2	-3.9629176504652506
2213	3	-1.863668285273679
2213	4	-3.5238189907980875
2213	5	-1.3379407971830126
2213	6	-1.0322792529849636
2213	7	-0.9134348920919493
2213	8	-0.853679373479622
2213	9	-0.8456644506848647
2213	10	-1.027482254907838
2213	11	-1.0611203257260655
2213	12	-0.8657625608846189
2213	13	-1.4086149152880623
2213	14	-6.164675729628126
2213	15	-2.89322751390781
2213	16	-2.2765371505539065
2213	17	-0.9833466583465196
2213	18	-0.26517647120605403
2213	19	-0.9865530985972129
2213	20	-0.9905479336879708
2213	21	-1.243822774396918
2213	22	-1.011334034942962
2213	23	-5.578042549812462
2213	24	-3.2028295966553557
2213	25	-2.3057615620879695
2214	1	0.033689423930468365
2214	2	0.26237442242956277
2214	3	0.03525803831017882
2214	4	0.37567810904148047
2214	5	0.09440936119454335
2214	6	-0.10444902549720325
2214	7	-0.34139367279045274
2214	8	-0.3105677687241837
2214	9	-0.1990058057385511
2214	10	0.30597448950781114
2214	11	-0.07711234514273713
2214	12	-0.07310346343099638
2214	13	-0.2794497912599212
2214	14	0.3803950260590696
2214	15	0.15405949913262873
2214	16	0.32114272109856756
2214	17	0.8219209380169659
2214	18	1.2324170126887992
2214	19	0.2856043328067912
2214	20	0.10456087374959822
2214	21	-0.05092611060984353
2214	22	0.45883133590734104
2214	23	1.8367832348763358
2214	24	0.7938588726358518
2214	25	0.7909875843187137
2215	1	-2.487891056106438
2215	2	-4.630915285298844
2215	3	-2.631196821276145
2215	4	-4.194908541124196
2215	5	-5.292330406638282
2215	6	-2.4257720102249145
2215	7	-1.6842228387363107
2215	8	-2.0452112009303924
2215	9	-1.5868439762576685
2215	10	-3.2278525428421183
2215	11	-2.008357105412614
2215	12	-1.6870379296549771
2215	13	-1.7259906822247402
2215	14	-0.9151118914936376
2215	15	-1.570051243811207
2215	16	-2.3563261066656835
2215	17	-6.633533038855035
2215	18	-2.9394838394188483
2215	19	-2.2618875830381526
2215	20	-1.936289060379508
2215	21	-1.9000867557453773
2215	22	-1.680322589066371
2215	23	-7.001072345962996
2215	24	-4.061004484073884
2215	25	-3.9357552220031202
2216	1	-1.2908229003738718
2216	2	-0.741526108717224
2216	3	-0.9728578146476039
2216	4	-3.488765161183821
2216	5	-1.5981888110209326
2216	6	-1.3406738110148346
2216	7	-1.2625935748555825
2216	8	-1.0569776561490443
2216	9	-1.1417045360828695
2216	10	-0.8593093467410853
2216	11	-1.313396466202235
2216	12	-2.0169945258744733
2216	13	-1.4273045928168884
2216	14	-7.178025499291575
2216	15	-3.670703283616845
2216	16	-2.078454047050613
2216	17	-1.1523261876444693
2216	18	-0.2432679068397121
2216	19	-0.8794415366899884
2216	20	-1.4004532918302885
2216	21	-1.4477928192486047
2216	22	-0.9682646452651795
2216	23	0.15248204516749553
2216	24	-0.5916709999988399
2216	25	-0.5249529402324598
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75839.0
3	1.0
4	652.0
5	3794.0
6	4601.0
7	4220.0
8	3988.0
9	3835.0
10	3573.0
11	3309.0
12	3340.0
13	3145.0
14	3418.0
15	3432.0
16	3595.0
17	3709.0
18	4180.0
19	5082.0
20	6408.0
21	7927.0
22	9773.0
23	11725.0
24	13906.0
25	16411.0
26	18945.0
27	21727.0
28	24311.0
29	29460.0
30	34709.0
31	39331.0
32	50706.0
33	66613.0
34	87721.0
35	115461.0
36	163982.0
37	230727.0
38	588028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41643127008756	18.750782243243066	12.80267947878796	25.03010700788142
2	13.425223760712205	19.817950894145053	43.2987901299889	23.458035215153842
3	19.197762265885327	24.400470144394653	29.10412836569765	27.29763922402237
4	10.41005650380183	16.669579028125035	40.25958715481036	32.66077731326277
5	12.123332104504694	38.121196815259175	36.70776460111744	13.04770647911869
6	31.738816432303835	37.2128005724991	18.502527121606395	12.545855873590666
7	28.400292472541803	31.054803897400056	22.07144515421823	18.4734584758399
8	24.54428283186095	37.93606077505427	20.45114563562253	17.06851075746225
9	26.076047383903926	15.689455429784646	20.237793187307165	37.99670399900426
10	13.47511618749424	29.86308846287805	35.61184101074443	21.049954338883285
11	34.19946782571442	22.936747609733274	24.323248907857977	18.54053565669433
12	22.819004378685054	25.16960074864864	32.33639167152323	19.675003201143078
13	28.773207287522062	22.07431118556855	25.741645974810783	23.410835552098604
14	19.992999231112137	22.48407624256168	28.974686536085375	28.54823799024081
15	23.124359378636917	31.037218717482762	23.4095387286841	22.42888317519622
16	21.333650065550405	28.727113454557202	28.812140497465045	21.127095982427345
17	21.161874888176545	28.859158393476154	28.823494564557215	21.15547215379009
18	20.616658638815508	27.90329480962026	30.93136037672063	20.548686174843606
19	22.753298824092635	27.665001047245745	28.231471230663356	21.350228897998264
20	22.770463768428133	27.75765278341238	28.902204494675154	20.569678953484335
21	22.379329287339374	27.28287290305053	27.93674653734805	22.401051272262045
22	22.04827936609768	28.477309463950263	28.047801802890877	21.426609367061186
23	20.620726342931377	27.73443741065226	29.97697977247853	21.667856473937835
24	22.038556281973634	27.83360903173604	28.800867552253028	21.326967134037304
25	22.005552194397417	27.587907251185033	28.557775914678846	21.848764639738704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	266.5
3	390.0
4	390.0
5	390.0
6	814.0
7	1238.0
8	1238.0
9	1238.0
10	2132.0
11	3026.0
12	3026.0
13	3026.0
14	5569.0
15	8112.0
16	8112.0
17	8112.0
18	14646.5
19	21181.0
20	21181.0
21	21181.0
22	33314.0
23	45447.0
24	45447.0
25	45447.0
26	64148.5
27	82850.0
28	82850.0
29	82850.0
30	106355.0
31	129860.0
32	129860.0
33	129860.0
34	149389.0
35	168918.0
36	168918.0
37	168918.0
38	183723.0
39	198528.0
40	198528.0
41	198528.0
42	208804.5
43	219081.0
44	219081.0
45	219081.0
46	225987.0
47	232893.0
48	232893.0
49	232893.0
50	218792.0
51	204691.0
52	204691.0
53	204691.0
54	176440.0
55	148189.0
56	148189.0
57	148189.0
58	123337.0
59	98485.0
60	98485.0
61	98485.0
62	78310.5
63	58136.0
64	58136.0
65	58136.0
66	43908.5
67	29681.0
68	29681.0
69	29681.0
70	21400.5
71	13120.0
72	13120.0
73	13120.0
74	9034.5
75	4949.0
76	4949.0
77	4949.0
78	3366.0
79	1783.0
80	1783.0
81	1783.0
82	1194.0
83	605.0
84	605.0
85	605.0
86	403.5
87	202.0
88	202.0
89	202.0
90	129.5
91	57.0
92	57.0
93	57.0
94	33.5
95	10.0
96	10.0
97	10.0
98	9.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10211870896108123
2	0.049414208319773334
3	0.03637268602714551
4	0.032663629228324755
5	0.0368512740011869
6	0.018844401477879663
7	0.01890422497463484
8	0.022254340792924553
9	0.027997396481421213
10	0.0345779811244903
11	0.019562283438941744
12	0.018305990007083103
13	0.013998698240710606
14	0.02028016540000383
15	0.021237341348086605
16	0.020998047361065912
17	0.025305339127438403
18	0.01908369546490036
19	0.03194574726726267
20	0.02165610582537282
21	0.027877749487910868
22	0.0359539215498593
23	0.026621456056052224
24	0.04044068380649731
25	0.031885923770507495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1671584.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.06281989394117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.77439599302903	74.49202909357236
2	6.9437931181989265	11.396544880790934
3	1.237324743543778	3.0461507273925
4	0.39551461054099263	1.2982817700099105
5	0.17342089925590706	0.7115704010741409
6	0.1079589595107035	0.5315649990158489
7	0.07066016934630186	0.4059000925518666
8	0.04812897338720334	0.3159679419803484
9	0.03921844406978818	0.2896538500997669
>10	0.1832819705677626	2.692173802878962
>50	0.014058030901285335	0.8033105284368606
>100	0.010105319202838164	1.7713701730035232
>500	9.587582686198975E-4	0.5657582690414215
>1k	0.0011800101767629506	1.6797234701514347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3890	0.23271340237762508	No Hit
CCCATGTACTCTGCGTTGATACCAC	3245	0.19412724697053813	No Hit
GTCCTACAGTGGACATTTCTAAATT	2362	0.14130309933571988	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2188	0.1308938109003197	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1843	0.11025470451978482	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1832	0.1095966460554779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	5.9823496755173535E-5	0.0	0.0	0.0
9	0.0	5.9823496755173535E-5	0.0	5.9823496755173535E-5	0.0
10	0.0	5.9823496755173535E-5	0.0	5.9823496755173535E-5	0.0
11	0.0	5.9823496755173535E-5	0.0	5.9823496755173535E-5	0.0
12	0.0	5.9823496755173535E-5	0.0	5.9823496755173535E-5	5.9823496755173535E-5
13	0.0	5.9823496755173535E-5	0.0	5.9823496755173535E-5	5.9823496755173535E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	655	0.0	13.202611	4
GACGTAT	55	0.0030415657	12.105209	1
GTACTAG	120	9.840733E-9	11.889044	1
GGCGAGG	475	0.0	11.799461	19
GTCCTAC	1505	0.0	11.501959	1
GTAGGAC	1495	0.0	11.441371	3
TGTAGGA	1625	0.0	11.344416	2
AGGACGT	1225	0.0	11.247414	5
GGACGTG	1165	0.0	11.08929	6
TTAGGAC	720	0.0	11.086476	3
TGGCGAG	1170	0.0	11.042231	18
TCTAGAC	190	0.0	11.003119	3
TGGCGGG	95	1.3609426E-5	10.999497	18
GTCCTAA	575	0.0	10.917244	1
TAGACAG	270	0.0	10.909847	5
GTATTAG	280	0.0	10.869984	1
GACCGTG	70	0.0014939354	10.855023	7
CCGTGCA	70	0.0014939354	10.855023	9
GTGCTAG	255	0.0	10.816699	1
TAGGACG	1255	0.0	10.752375	4
>>END_MODULE