FastQCFastQC Report
Thu 26 May 2016
SRR1513103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671584
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC46110.27584614353810516No Hit
CCCATGTACTCTGCGTTGATACCAC40720.24360127878706664No Hit
GTATCAACGCAGAGTACTTTTTTTT33540.20064800811685204No Hit
GTCCTACAGTGGACATTTCTAAATT23080.13807263051094054No Hit
TATCAACGCAGAGTACTTTTTTTTT22690.13573951413748875No Hit
CTGTAGGACGTGGAATATGGCAAGA19990.11958717001359188No Hit
GGTATCAACGCAGAGTACTTTTTTT19860.11880946455577464No Hit
GTCCTACAGTGTGCATTTCTCATTT18250.10917788157819171No Hit
GAGTACATGGGAAGCAGTGGTATCA17110.10235800294810192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG350.002141781416.3179825
CCGTTTA350.002180300416.27449813
TAGGACC6650.013.4551784
GGCGAGG4300.012.57531419
TCCAACG1251.4333636E-912.14798818
AGGACGT9250.012.0400265
GGACGTG9250.011.93711956
ATAGGGG802.8091656E-511.8985293
TAGGACG9550.011.4624574
TGTAGGA14150.011.4432562
TTAGGAC6400.011.3036023
GACGTGA5300.011.2779247
TGGCGAG11800.011.099195518
GTAGGAC13400.011.08161453
CTGTAGG13850.011.0751771
GTCCTAC13200.010.8987731
ATTAGAC1053.3848628E-610.8786553
CTAGGAC3100.010.7470593
GACGTGG4600.010.7253137
TCTAGAC2050.010.6796553
Produced by FastQC (version 0.11.5)