FastQCFastQC Report
Thu 26 May 2016
SRR1513102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790977
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC89100.49749382599553205No Hit
CCCATGTACTCTGCGTTGATACCAC72870.40687289674853444No Hit
GAGTACATGGGAAGCAGTGGTATCA38000.21217469571077682No Hit
GTCCTACAGTGGACATTTCTAAATT24780.13836023578192239No Hit
GCGTTGATACCACTGCTTCCCATGT24580.13724352685712882No Hit
CATGTACTCTGCGTTGATACCACTG24340.13590347614737655No Hit
GTATCAACGCAGAGTACATGGGAAG24270.1355126280236988No Hit
CTGTAGGACGTGGAATATGGCAAGA19570.10926996829105008No Hit
GTCCTAAAGTGTGTATTTCTCATTT19030.10625485419410746No Hit
ACGCAGAGTACATGGGAAGCAGTGG18270.10201136027989192No Hit
TATCAACGCAGAGTACATGGGAAGC18040.10072714501637933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG508.705174E-515.2020344
GCGTTTA1400.013.56983213
TCCAACG852.6999805E-713.41168618
TAGGACC7500.013.17509654
CGACAGT604.096629E-412.6651766
GTCCTAG2350.012.5490231
GTAGGAC16450.012.3008393
TGTAGGA17700.012.0245442
CGTTTAT1603.6379788E-1211.87360314
CTGTAGG16300.011.847441
AGGACCT11500.011.7310215
GGACCTG11300.011.6004046
GTCCTAC12800.011.2223311
GGACACG600.00581687711.0984641
CCAACGA600.005876506711.08326819
TGGCGAG12550.011.05172718
TAGGACT3550.010.97334
AGCACCG700.001490971810.8576865
ATAGACC1752.0008883E-1110.85707953
CGAAATC700.001493058510.855865513
Produced by FastQC (version 0.11.5)