##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513102_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1790977
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.807410703766713	31.0	29.0	34.0	22.0	34.0
2	28.5040796168795	31.0	28.0	34.0	16.0	34.0
3	28.87814751389884	31.0	29.0	34.0	18.0	34.0
4	31.70934970130828	35.0	32.0	37.0	17.0	37.0
5	31.93549721744054	35.0	32.0	37.0	19.0	37.0
6	32.39828540511687	35.0	33.0	37.0	24.0	37.0
7	32.40154731188619	35.0	33.0	37.0	23.0	37.0
8	32.373293459379994	36.0	33.0	37.0	23.0	37.0
9	33.682137179874445	38.0	34.0	39.0	22.0	39.0
10	33.15830242376089	38.0	33.0	39.0	17.0	39.0
11	33.545370487728206	38.0	33.0	39.0	22.0	39.0
12	33.41039611340626	38.0	33.0	39.0	18.0	39.0
13	33.46764531314472	38.0	34.0	39.0	19.0	39.0
14	33.74090007856047	39.0	33.0	41.0	10.0	41.0
15	34.24385516955271	39.0	33.0	41.0	17.0	41.0
16	33.800739484649995	39.0	33.0	41.0	11.0	41.0
17	33.32459210810636	38.0	32.0	41.0	10.0	41.0
18	32.69116968001264	38.0	31.0	41.0	10.0	41.0
19	33.601981488316156	39.0	32.0	41.0	11.0	41.0
20	33.876557320389935	39.0	33.0	41.0	10.0	41.0
21	33.902357763388366	39.0	34.0	41.0	10.0	41.0
22	33.47068108635678	39.0	33.0	41.0	2.0	41.0
23	31.717281126446625	38.0	30.0	40.0	2.0	41.0
24	32.79234239189001	38.0	32.0	41.0	2.0	41.0
25	32.50616172066978	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0149542570160293
1101	2	1.4098615090089588
1101	3	1.1097863013598186
1101	4	0.9090192490943885
1101	5	1.3161669902863515
1101	6	0.9767660066994637
1101	7	1.0106583165491188
1101	8	0.9874422975593262
1101	9	1.1357838170971633
1101	10	1.5872649473061813
1101	11	1.0803831351695408
1101	12	1.137518916727167
1101	13	1.1131022137553401
1101	14	0.1148599689781662
1101	15	0.9205769922394182
1101	16	1.6087738013657358
1101	17	2.1973708498343356
1101	18	-3.7517508647732143
1101	19	-0.3456902858973834
1101	20	0.6546416743023045
1101	21	0.9849447204488797
1101	22	1.599152685913218
1101	23	3.3556655130769144
1101	24	2.273429746205359
1101	25	2.4507237758061535
1102	1	1.035202512923199
1102	2	1.2912247911474353
1102	3	1.0413019003172757
1102	4	1.5519748292592688
1102	5	1.4320869876017035
1102	6	1.025800836354449
1102	7	0.9740064527654724
1102	8	0.7726058563662974
1102	9	1.0191497764321156
1102	10	1.2306955620546205
1102	11	0.9614197844232422
1102	12	1.0293978388386478
1102	13	0.9757269849072401
1102	14	1.8176370942359128
1102	15	1.3291637829967513
1102	16	1.6066864829993506
1102	17	1.2144638917335797
1102	18	1.643615497259816
1102	19	1.3914213248576388
1102	20	1.2996055246925096
1102	21	1.3890250214284094
1102	22	1.7568615575755757
1102	23	3.4030067735964664
1102	24	2.3457938449260496
1102	25	2.623162448244827
1103	1	0.7295842932642529
1103	2	1.0914062618540932
1103	3	0.8629150614802761
1103	4	1.2209620525873284
1103	5	1.1972786376808138
1103	6	0.864289639044749
1103	7	0.7892205031349349
1103	8	0.7595762596282327
1103	9	0.8254774331349068
1103	10	1.164483706346516
1103	11	0.940549518965625
1103	12	1.0747099400992965
1103	13	0.8590957617379686
1103	14	1.762895321380526
1103	15	1.253362334633671
1103	16	1.6163021620684006
1103	17	1.9483405029949736
1103	18	1.932145981503055
1103	19	1.525781101875502
1103	20	1.3488691505906445
1103	21	1.4327401804704678
1103	22	1.8086419553366824
1103	23	3.204097866220252
1103	24	2.1362303386851593
1103	25	2.3714909329077187
1104	1	0.6033372892241147
1104	2	1.145283928979893
1104	3	0.9134305382326104
1104	4	1.4589034202427733
1104	5	1.24977023126171
1104	6	0.8824281883003522
1104	7	0.8568068677024598
1104	8	0.7889422309568062
1104	9	0.9719885004077611
1104	10	1.3535001487099692
1104	11	0.8963849300888498
1104	12	1.0845527976894829
1104	13	0.8654636265622244
1104	14	1.820466040746659
1104	15	1.114753848347462
1104	16	1.5309552683029821
1104	17	2.049022108621962
1104	18	2.6939148739231484
1104	19	1.8304056604276795
1104	20	1.199353917929109
1104	21	1.3934038358037029
1104	22	1.7092883243634844
1104	23	3.10814264311108
1104	24	2.2019224530433945
1104	25	2.360310669025573
1105	1	0.8363733671481555
1105	2	1.1742649492312225
1105	3	0.8914196321104022
1105	4	1.1861530613432656
1105	5	1.218191865853175
1105	6	0.7966181258453844
1105	7	0.7569662762139515
1105	8	0.7740223314302455
1105	9	0.999280343299155
1105	10	1.3687196993806765
1105	11	0.9042399918158779
1105	12	0.931134729057213
1105	13	0.9951716601983378
1105	14	1.8334085375815263
1105	15	1.216181890608226
1105	16	1.667153531948074
1105	17	2.0933118745955284
1105	18	1.8982412024963438
1105	19	1.4677817041848584
1105	20	1.2193004377556562
1105	21	1.201660643837151
1105	22	1.8023143402011357
1105	23	3.230041686844295
1105	24	2.2977334185061125
1105	25	2.2727926319064267
1106	1	-0.3254665407774624
1106	2	0.15015184217218547
1106	3	-0.18754092033588776
1106	4	-2.6067769952253137
1106	5	-0.4089057600720807
1106	6	-0.2986480172610726
1106	7	-0.2822097182495469
1106	8	-0.24607192445228776
1106	9	-0.23412821420220098
1106	10	0.04892596404939553
1106	11	-0.17633374291362003
1106	12	-0.09251650161017722
1106	13	-0.15315422594235883
1106	14	0.555490968224845
1106	15	-0.1583799286130514
1106	16	-1.2570724113629979
1106	17	-6.183642825410491
1106	18	-7.0629773538014256
1106	19	-3.0655877367491264
1106	20	-1.6221164846731781
1106	21	-1.270724187961605
1106	22	-1.5643229890400008
1106	23	-4.831179126178512
1106	24	-2.0905286023159384
1106	25	-0.7186205293140127
1107	1	0.4272779910828213
1107	2	0.9522117605486429
1107	3	0.6144153744016236
1107	4	0.85275050090873
1107	5	1.065574427885256
1107	6	0.5573679328082761
1107	7	0.5755877613047105
1107	8	0.5212865296424383
1107	9	0.5602227032655591
1107	10	0.8717325305996795
1107	11	0.6276566639287324
1107	12	0.6906938782900269
1107	13	0.6510244969361452
1107	14	1.4573050793731497
1107	15	0.831025052495562
1107	16	-5.540185145911117
1107	17	-0.9476942904893235
1107	18	0.813467897047488
1107	19	0.8325661682678529
1107	20	0.69084882532092
1107	21	0.839185121426695
1107	22	1.069123333915023
1107	23	2.820902015116303
1107	24	1.8412750866856058
1107	25	1.620311469665694
1108	1	0.23688435209771797
1108	2	0.4888709602054391
1108	3	0.24980656365498177
1108	4	0.9022877331749832
1108	5	0.5363858131659036
1108	6	0.24869381050694273
1108	7	0.11626537416909599
1108	8	0.1814000216923901
1108	9	0.3067975882744207
1108	10	0.7187376421295113
1108	11	0.3668304340049531
1108	12	0.38586839425472164
1108	13	0.3220314951250529
1108	14	0.9357607246032416
1108	15	0.6148470727808046
1108	16	0.9481320711936192
1108	17	0.9803572099408981
1108	18	0.8065332061646373
1108	19	0.83087914404598
1108	20	0.7251387321823657
1108	21	0.6967228607021099
1108	22	0.9625114957386955
1108	23	2.7220993367429287
1108	24	1.5281379431459499
1108	25	1.6360728731060448
1109	1	0.324054826614411
1109	2	0.7075001341948912
1109	3	0.5517397798236985
1109	4	0.5036937085704203
1109	5	0.3279499607933225
1109	6	0.2588871321238102
1109	7	0.19148505782462877
1109	8	0.08860564559397233
1109	9	0.2698907341443686
1109	10	0.829106710670807
1109	11	0.39530143576241983
1109	12	0.42066628529162386
1109	13	0.26579247930782657
1109	14	-0.9085275003528182
1109	15	-0.09341303266734968
1109	16	-0.30107706764645314
1109	17	0.8169125702220938
1109	18	1.642034084899258
1109	19	0.8705979489668891
1109	20	0.7497433016024004
1109	21	0.6661428958514932
1109	22	0.387116065158736
1109	23	-4.269113317035632
1109	24	-1.4451485243408655
1109	25	-0.27886561547040856
1110	1	0.3616187953126051
1110	2	0.8850720166083725
1110	3	0.5862288077997029
1110	4	1.1554220267618156
1110	5	0.7033262975837538
1110	6	0.4365971142729208
1110	7	0.4280156802401365
1110	8	0.32128316529870204
1110	9	0.42669806039538116
1110	10	0.8062382350577195
1110	11	0.4618018623705211
1110	12	0.4341331710765388
1110	13	0.3147684097247705
1110	14	1.2585554069228166
1110	15	0.9024041064705344
1110	16	1.126330085799765
1110	17	1.477426553420763
1110	18	2.0760747090380107
1110	19	1.2719710205992243
1110	20	0.9304583339988
1110	21	0.7324084328222966
1110	22	1.0326666417851698
1110	23	2.3733274937178948
1110	24	1.274620092554251
1110	25	1.7373809177759014
1111	1	0.38388398090291176
1111	2	0.7532755409174818
1111	3	0.5494637190054092
1111	4	1.0049912085914379
1111	5	0.815970939906709
1111	6	0.2311281276466488
1111	7	0.15863131458331736
1111	8	-0.030170444655617246
1111	9	0.17791875274495084
1111	10	0.3919961198464108
1111	11	0.16855546875191152
1111	12	0.3756194534813133
1111	13	0.2716089457014803
1111	14	-0.5507628618833422
1111	15	-0.0732977580612797
1111	16	0.49095017252619755
1111	17	0.938385648988671
1111	18	1.5718991694031388
1111	19	0.644443611213255
1111	20	0.4607955367597114
1111	21	0.4533938559726778
1111	22	0.6579121638893426
1111	23	0.4451280701037277
1111	24	0.4007485718106025
1111	25	-0.023985725219134224
1112	1	0.27736906272027184
1112	2	0.670957478510644
1112	3	0.41595607256180855
1112	4	0.90498050274687
1112	5	0.7371166299807435
1112	6	0.09943718067352592
1112	7	0.20329609811937388
1112	8	0.2177472685759838
1112	9	0.15942728859717192
1112	10	0.66179987348022
1112	11	0.31727282622006214
1112	12	0.37160476176845947
1112	13	0.1811157356775155
1112	14	0.4825018442412201
1112	15	0.21122323774697094
1112	16	0.6751508165239244
1112	17	0.9007858993407325
1112	18	1.0148740765505266
1112	19	0.661577080153954
1112	20	0.40924195400765484
1112	21	0.5569432552100935
1112	22	0.5849230097334157
1112	23	2.0886275636349296
1112	24	1.086028859056647
1112	25	1.3065053436359122
1113	1	0.26697295530494713
1113	2	0.6876498535471214
1113	3	0.38856028079365146
1113	4	0.4495600071212671
1113	5	0.5400509575033858
1113	6	0.21809073706418758
1113	7	0.12210211804683269
1113	8	0.1409818777988363
1113	9	0.333844979131932
1113	10	0.45998222225247076
1113	11	0.2661475142951488
1113	12	0.18264109692619712
1113	13	0.1460729446419009
1113	14	0.7781351932576541
1113	15	0.303983601991888
1113	16	0.7829044211550027
1113	17	1.0855073853837638
1113	18	1.6521811893318485
1113	19	0.7036760742519448
1113	20	0.44719405901691545
1113	21	0.46712257853403827
1113	22	0.7455838438757212
1113	23	0.6858790510477561
1113	24	0.6240738715336391
1113	25	0.9840675946171018
1114	1	-0.8992118301827432
1114	2	-3.2641793638605883
1114	3	-1.486447489857838
1114	4	-0.491064475087267
1114	5	-0.20527812505177323
1114	6	-0.43944231995243754
1114	7	-0.5448718269889561
1114	8	-0.5166459993075527
1114	9	-0.46014736995113736
1114	10	-0.407596200855572
1114	11	-0.682403153376157
1114	12	-0.6263125077490201
1114	13	-0.604764290143045
1114	14	0.10151003860052299
1114	15	-0.45643590433892456
1114	16	-0.09562983441658446
1114	17	0.15920799336921476
1114	18	0.23952681944400211
1114	19	-0.35458724258865715
1114	20	-0.5195244523076781
1114	21	-0.7772661644829597
1114	22	-0.7062658130666435
1114	23	0.9971793320006483
1114	24	0.025905447349195754
1114	25	-0.07040678884066409
1115	1	-0.37062081126995494
1115	2	-0.0901189439675889
1115	3	-0.2664936428797269
1115	4	-0.2529821089093467
1115	5	-0.1540212269736685
1115	6	-0.6012940026625309
1115	7	-0.754060334912019
1115	8	-0.8039212616450051
1115	9	-0.7870185573517929
1115	10	-0.5198728026256632
1115	11	-0.6415861760036421
1115	12	-0.7952400941934457
1115	13	-0.89115080703138
1115	14	0.08256905782967294
1115	15	-0.7169329741705681
1115	16	-0.12175170492788823
1115	17	0.4206019728108359
1115	18	0.74808324272513
1115	19	-0.090858313139762
1115	20	-0.4705035137863618
1115	21	-0.47057474169933755
1115	22	-0.2598186262578892
1115	23	1.5863008427786127
1115	24	0.17816424104340456
1115	25	-0.2238946714400285
1116	1	-0.4934236501857221
1116	2	-0.6010454758354769
1116	3	-0.5704964333201374
1116	4	0.2104191520714096
1116	5	-0.37041863789243124
1116	6	-0.43905350895966677
1116	7	-0.6943265764870965
1116	8	-0.7102987230900055
1116	9	-0.8285300873397077
1116	10	-0.41890609214473074
1116	11	-0.7316897454678966
1116	12	-0.7617108396263532
1116	13	-0.9021298538691411
1116	14	-0.41139653709657154
1116	15	-1.0368521508352728
1116	16	-0.5425928455903275
1116	17	-0.2441872059979744
1116	18	0.32605787395506525
1116	19	-0.529189269316177
1116	20	-0.7425400691772239
1116	21	-0.9403309355137566
1116	22	-0.5059736914665649
1116	23	0.7135340496409839
1116	24	-0.2609202583397803
1116	25	-0.008717379037300077
1201	1	1.2453336300265505
1201	2	1.6190498845115116
1201	3	1.3547673066891939
1201	4	1.9202157095408943
1201	5	1.6656703397008776
1201	6	1.2485282286465278
1201	7	1.1166586790359858
1201	8	1.1714538581729528
1201	9	1.4880915689582395
1201	10	1.823010740958864
1201	11	1.481106083689994
1201	12	1.5925244014559325
1201	13	1.4913208548602697
1201	14	2.4205723928181087
1201	15	1.8486206578070892
1201	16	2.1861156549348877
1201	17	2.506674944269598
1201	18	3.1780252521618735
1201	19	2.281848948691348
1201	20	1.9742795924792205
1201	21	1.7903916880309154
1201	22	2.193224948977168
1201	23	3.7748373510775863
1201	24	2.524617762297318
1201	25	2.811929503508793
1202	1	0.9137218982971795
1202	2	1.15582082056698
1202	3	0.9762020748994154
1202	4	1.5776512155428115
1202	5	1.3385687081058286
1202	6	0.9713420441773053
1202	7	0.6950974923482605
1202	8	0.8429593358030232
1202	9	0.9281361993084261
1202	10	1.3492130607431818
1202	11	0.9738251234880195
1202	12	1.085820186117516
1202	13	1.042077886723746
1202	14	1.9949338947106412
1202	15	1.3412964450270124
1202	16	1.5128641666978098
1202	17	1.976743568625956
1202	18	2.599811378549056
1202	19	1.8353132764233635
1202	20	1.561345520319989
1202	21	1.6010516211432346
1202	22	1.9025414622724668
1202	23	3.502043735421047
1202	24	2.4832608040289514
1202	25	2.4961783212607003
1203	1	1.1613372460616382
1203	2	1.535543394782401
1203	3	1.2454967625974724
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2211	16	-0.5212744449615769
2211	17	0.09572830633133123
2211	18	-5.46929008231243
2211	19	-1.8634943967247537
2211	20	-1.204180312426665
2211	21	-0.86297019723348
2211	22	-0.7344143337003146
2211	23	-4.9805782766552475
2211	24	-2.449867384068515
2211	25	-1.7810219559395755
2212	1	0.04401449614310238
2212	2	0.30346162020999756
2212	3	-0.038838594669453386
2212	4	0.5280850706929492
2212	5	0.5586200044846628
2212	6	0.011068313597888846
2212	7	-0.22360258888740248
2212	8	-0.2710179118244511
2212	9	-0.5725839863161397
2212	10	-3.9452987223878786
2212	11	-1.3635134000362754
2212	12	-0.6134778197316066
2212	13	-0.4045281578788291
2212	14	0.276796854915645
2212	15	-0.13243444099101254
2212	16	-0.014327168130954249
2212	17	0.557701822387557
2212	18	1.064647477075816
2212	19	0.3464745248030212
2212	20	-0.014351769469804765
2212	21	-0.16185522305381284
2212	22	0.08494765003070626
2212	23	1.4230523708778406
2212	24	0.6878718316134353
2212	25	0.45764880553075926
2213	1	-1.526606368752681
2213	2	-4.016380973981189
2213	3	-1.8897091962798562
2213	4	-3.5778408431997946
2213	5	-1.4128878768245343
2213	6	-0.9778206214648506
2213	7	-0.9885281278904721
2213	8	-0.8181509377155152
2213	9	-0.7843423750234635
2213	10	-0.9766942526502405
2213	11	-0.9901659778025831
2213	12	-0.7850818349926527
2213	13	-1.177982216516007
2213	14	-6.366963133589586
2213	15	-2.884357088545734
2213	16	-2.090483290978405
2213	17	-0.7288585781059282
2213	18	-0.08962371511488954
2213	19	-0.9070186422440116
2213	20	-1.0061292565202464
2213	21	-1.2524858398663241
2213	22	-1.0566786659397067
2213	23	-5.658871159436909
2213	24	-3.4078766525515967
2213	25	-2.6163973823374462
2214	1	-0.2993332426306168
2214	2	-0.15894679728359407
2214	3	-0.344751218248156
2214	4	0.08974687294264427
2214	5	-0.2217796165112098
2214	6	-0.40759418193383823
2214	7	-0.6059693945338545
2214	8	-0.7825265925264127
2214	9	-0.44483663550175123
2214	10	0.0450994334371444
2214	11	-0.6192363349849259
2214	12	-0.46064047533759833
2214	13	-0.5389227574929123
2214	14	0.009242727549263918
2214	15	-0.4008368251647738
2214	16	0.11459953327693029
2214	17	0.3047089808292469
2214	18	1.0368423154455826
2214	19	-0.012104274398666348
2214	20	0.0026489387375647766
2214	21	-0.19747031744788046
2214	22	0.38728092838903905
2214	23	1.7127086875676767
2214	24	0.7019297603394676
2214	25	0.4799925366020119
2215	1	-2.8220676166076615
2215	2	-4.840716653259047
2215	3	-3.0237255391720375
2215	4	-4.457444356956707
2215	5	-5.244039788050795
2215	6	-2.738747433532506
2215	7	-2.2934982298675095
2215	8	-2.600901025543841
2215	9	-2.272920793468934
2215	10	-4.034503032415529
2215	11	-2.7549964876539796
2215	12	-2.331380002639669
2215	13	-2.2911907519606345
2215	14	-1.5123812373633498
2215	15	-2.1990478983950332
2215	16	-3.2219451715352605
2215	17	-7.5252971476018224
2215	18	-3.647193801989225
2215	19	-2.905703979248088
2215	20	-2.5903012859245074
2215	21	-2.635624126946876
2215	22	-2.355103526808332
2215	23	-6.974188562494646
2215	24	-4.402629438070992
2215	25	-4.233847216864071
2216	1	-1.4673905542339014
2216	2	-1.02541849738234
2216	3	-1.223329584781787
2216	4	-3.6076916997058923
2216	5	-1.8900449117688396
2216	6	-1.559648237816873
2216	7	-1.6484340691310102
2216	8	-1.5305710029346606
2216	9	-1.6547522096592573
2216	10	-1.3780596085590986
2216	11	-2.03652433988465
2216	12	-2.890238629187767
2216	13	-2.000992747407256
2216	14	-7.595907754833124
2216	15	-4.249961446797499
2216	16	-2.575599035236767
2216	17	-1.6201618908116266
2216	18	-0.8673020537694853
2216	19	-1.3728599938715575
2216	20	-1.9711770944212397
2216	21	-2.2163300889700785
2216	22	-1.7295113614395987
2216	23	-0.7354501448927984
2216	24	-1.2303318259798033
2216	25	-1.049476729467134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97111.0
3	0.0
4	778.0
5	4800.0
6	5937.0
7	5245.0
8	4767.0
9	4527.0
10	4370.0
11	4235.0
12	4182.0
13	4122.0
14	4267.0
15	4309.0
16	4519.0
17	4764.0
18	5379.0
19	6372.0
20	7928.0
21	10074.0
22	12337.0
23	14955.0
24	17773.0
25	20349.0
26	23424.0
27	26884.0
28	29778.0
29	35258.0
30	41482.0
31	47841.0
32	60751.0
33	78129.0
34	99705.0
35	129587.0
36	178387.0
37	242535.0
38	544116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25051076448377	18.07974913149076	12.02218017479185	25.647559929233616
2	13.909869568787675	19.754555372515767	41.85166626725634	24.483908791440218
3	19.500352446627517	24.16085865158724	28.792599402795666	27.546189498989577
4	10.905170054500996	16.149508552713154	39.17358215307418	33.77173923971167
5	12.440765705885113	37.96055292043781	36.41320011528485	13.185481258392223
6	32.75016670147758	36.19080701385995	18.019843339703545	13.039182944958913
7	28.664062347294085	30.923677060674688	21.77559106787333	18.636669524157902
8	25.44371494121405	36.966117938258556	20.306339231661084	17.283827888866302
9	26.822988372216415	15.01597640373693	19.524667779606613	38.63636744444004
10	14.030355619777794	29.249945959109898	35.162996820105555	21.556701601006754
11	34.66353328031596	22.52318765469606	23.70664042604523	19.106638638942748
12	23.443752251334047	24.736723062875395	31.751223748397887	20.06830093739267
13	29.31220405977357	21.439209779911778	25.17698475057952	24.071601409735123
14	20.7476327568923	21.893850333829445	28.0301689112776	29.328347998000652
15	24.107459492424567	30.021261136686157	22.79761263180704	23.073666739082235
16	22.023603419658794	27.88637028730227	28.33884735070267	21.75117894233626
17	22.23905171046429	28.123136003860356	28.220035878085998	21.417776407589354
18	22.030445286609904	26.78421241334017	30.273223494405855	20.912118805644074
19	23.551710578935964	27.07547190888232	27.592398966034175	21.78041854614754
20	23.61982737196009	27.198941110437826	27.88939742146988	21.291834096132206
21	22.700603588241897	26.84395453903161	27.533889354629178	22.921552518097315
22	22.91659451917373	27.80322334517856	26.781726557464218	22.498455578183492
23	21.494316557048897	27.203730429653987	29.02158323205605	22.280369781241063
24	22.764573694203367	27.602167097058533	27.68198928740401	21.951269921334085
25	22.737595481669977	26.897904150813563	27.479172205478562	22.885328162037897
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	138.0
2	249.0
3	360.0
4	360.0
5	360.0
6	692.0
7	1024.0
8	1024.0
9	1024.0
10	1717.5
11	2411.0
12	2411.0
13	2411.0
14	4451.5
15	6492.0
16	6492.0
17	6492.0
18	12051.0
19	17610.0
20	17610.0
21	17610.0
22	28896.0
23	40182.0
24	40182.0
25	40182.0
26	58185.0
27	76188.0
28	76188.0
29	76188.0
30	99807.5
31	123427.0
32	123427.0
33	123427.0
34	143217.0
35	163007.0
36	163007.0
37	163007.0
38	180416.0
39	197825.0
40	197825.0
41	197825.0
42	211887.0
43	225949.0
44	225949.0
45	225949.0
46	244587.0
47	263225.0
48	263225.0
49	263225.0
50	248376.5
51	233528.0
52	233528.0
53	233528.0
54	203384.0
55	173240.0
56	173240.0
57	173240.0
58	146571.5
59	119903.0
60	119903.0
61	119903.0
62	97324.5
63	74746.0
64	74746.0
65	74746.0
66	57536.0
67	40326.0
68	40326.0
69	40326.0
70	29690.5
71	19055.0
72	19055.0
73	19055.0
74	13391.5
75	7728.0
76	7728.0
77	7728.0
78	5399.0
79	3070.0
80	3070.0
81	3070.0
82	2052.5
83	1035.0
84	1035.0
85	1035.0
86	704.5
87	374.0
88	374.0
89	374.0
90	239.5
91	105.0
92	105.0
93	105.0
94	64.5
95	24.0
96	24.0
97	24.0
98	14.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11122420890943882
2	0.04679010394885026
3	0.035455508362195604
4	0.030765330878062647
5	0.03512049568475754
6	0.019598241630127022
7	0.02021243153876348
8	0.023060039296987064
9	0.029257773829591337
10	0.03718640719562563
11	0.020603279662441226
12	0.02077078600116026
13	0.015968937624547944
14	0.02188749492595382
15	0.021552482248515754
16	0.02171998858723479
17	0.02618682428640904
18	0.01982158341508573
19	0.03288707783517041
20	0.023562558313144168
21	0.028922761152153267
22	0.03701890085690659
23	0.02864358392095488
24	0.04176491378727923
25	0.03244039426525299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1790977.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.12050235467483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.84782289764195	67.74868232630652
2	8.698712087278695	13.416980920192302
3	1.9262141858603532	4.456518169687542
4	0.6318259151487363	1.9490692790789081
5	0.2785103972994163	1.0739430875365528
6	0.1615250235736311	0.7474134576509475
7	0.0937968728781979	0.5063563368964937
8	0.060869513712963105	0.3755429980502786
9	0.04882280781497193	0.33887155185507417
>10	0.2226700710251369	3.0758510664935863
>50	0.015070222326375645	0.800325957537795
>100	0.010932491305758628	1.791992908514415
>500	0.00190716744275365	1.018767130028805
>1k	0.0011736415032330152	1.7834223189121052
>5k	1.467051879041269E-4	0.9162624912586235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8910	0.49749382599553205	No Hit
CCCATGTACTCTGCGTTGATACCAC	7287	0.40687289674853444	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3800	0.21217469571077682	No Hit
GTCCTACAGTGGACATTTCTAAATT	2478	0.13836023578192239	No Hit
GCGTTGATACCACTGCTTCCCATGT	2458	0.13724352685712882	No Hit
CATGTACTCTGCGTTGATACCACTG	2434	0.13590347614737655	No Hit
GTATCAACGCAGAGTACATGGGAAG	2427	0.1355126280236988	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1957	0.10926996829105008	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1903	0.10625485419410746	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1827	0.10201136027989192	No Hit
TATCAACGCAGAGTACATGGGAAGC	1804	0.10072714501637933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.583544623967812E-5
13	0.0	0.0	0.0	5.583544623967812E-5	1.1167089247935624E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACG	50	8.705174E-5	15.202034	4
GCGTTTA	140	0.0	13.569832	13
TCCAACG	85	2.6999805E-7	13.411686	18
TAGGACC	750	0.0	13.1750965	4
CGACAGT	60	4.096629E-4	12.665176	6
GTCCTAG	235	0.0	12.549023	1
GTAGGAC	1645	0.0	12.300839	3
TGTAGGA	1770	0.0	12.024544	2
CGTTTAT	160	3.6379788E-12	11.873603	14
CTGTAGG	1630	0.0	11.84744	1
AGGACCT	1150	0.0	11.731021	5
GGACCTG	1130	0.0	11.600404	6
GTCCTAC	1280	0.0	11.222331	1
GGACACG	60	0.005816877	11.098464	1
CCAACGA	60	0.0058765067	11.083268	19
TGGCGAG	1255	0.0	11.051727	18
TAGGACT	355	0.0	10.9733	4
AGCACCG	70	0.0014909718	10.857686	5
ATAGACC	175	2.0008883E-11	10.8570795	3
CGAAATC	70	0.0014930585	10.8558655	13
>>END_MODULE