FastQCFastQC Report
Thu 26 May 2016
SRR1513102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790977
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC104330.5825312106185618No Hit
CCCATGTACTCTGCGTTGATACCAC100600.5617045891711618No Hit
GAGTACATGGGAAGCAGTGGTATCA41580.2321637854645816No Hit
CATGTACTCTGCGTTGATACCACTG29250.1633186802510585No Hit
GTATCAACGCAGAGTACATGGGAAG26560.1482989452125851No Hit
GTCCTACAGTGGACATTTCTAAATT26130.14589802102427893No Hit
GCGTTGATACCACTGCTTCCCATGT25440.14204537523374114No Hit
GTATCAACGCAGAGTACTTTTTTTT25010.13964445104543496No Hit
ACGCAGAGTACATGGGAAGCAGTGG24470.13662933694849236No Hit
TATCAACGCAGAGTACATGGGAAGC22720.12685813385654868No Hit
CTGTAGGACGTGGAATATGGCAAGA21580.12049289298522539No Hit
GCTTCCCATGTACTCTGCGTTGATA20840.11636106996348919No Hit
ACTCTGCGTTGATACCACTGCTTCC20350.11362513309774497No Hit
GTCCTACAGTGTGCATTTCTCATTT19300.10776241124257878No Hit
CTTTAGGACGTGAAATATGGCGAGG18680.10430061357571874No Hit
GTCCTAAAGTGTGTATTTCTCATTT18300.10217886661861096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6950.013.1510274
TTTATAC2450.012.04672053
GGCGAGG4950.011.69043319
TGGCGAG12150.011.5591218
GTAGGAC15800.011.1477663
AATAGCG600.005781369311.10759455
TCCGTGC600.005933844511.0688188
GGCGAGA8000.010.90947319
CCGGTTT700.001502026810.84807412
TGCACTG3450.010.7626385
AGGACCT11100.010.7216175
GTCCTAA7400.010.6880161
CGGTTTC803.800506E-410.67857313
TGTAGGA16800.010.65992
TAGGACA5550.010.6358444
TATACTG3850.010.63361555
CCTATAC2001.8189894E-1210.4728753
GTATTGG1553.4815457E-910.4512421
GTCCTAC15750.010.3458511
TAGGACT3600.010.3141964
Produced by FastQC (version 0.11.5)