FastQCFastQC Report
Thu 26 May 2016
SRR1513101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313796
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC84140.3636448502806643No Hit
CCCATGTACTCTGCGTTGATACCAC69730.30136623972035564No Hit
GAGTACATGGGAAGCAGTGGTATCA34080.1472904266408966No Hit
GCGTTGATACCACTGCTTCCCATGT23700.10242908190696155No Hit
GTCCTACAGTGGACATTTCTAAATT23140.10000881668046793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7750.012.8725924
GCCGGTT1155.338734E-912.38965411
CGAAATC1201.00426405E-811.8734213
CCGGTTT1052.7265924E-711.76033912
GTAGGAC15150.011.5391623
GTCCTAC16500.011.3573641
TGTAGGA16900.011.1878042
AGGACGT10800.010.9965225
GACGTGA5800.010.8086727
TGCCGGT1158.839288E-710.73746910
GGCGAGG5400.010.73005319
CTGTAGG16250.010.7125541
AAGTCGT803.7775715E-410.6858467
GGACGTG10850.010.6812986
TGGCGAG12850.010.64449718
TTACTCT3400.010.6189884
GTCCTAA7200.010.5694581
TCCTACA19450.010.4537632
ATAGGAC2550.010.4324643
TCCAACG1659.313226E-1010.36225618
Produced by FastQC (version 0.11.5)