##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513101_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2313796
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.315958710275236	31.0	30.0	34.0	25.0	34.0
2	29.005580440107945	31.0	30.0	34.0	16.0	34.0
3	29.380905231057536	31.0	30.0	34.0	23.0	34.0
4	32.26243886669352	35.0	33.0	37.0	19.0	37.0
5	32.48526317791197	35.0	33.0	37.0	22.0	37.0
6	32.94329664326501	37.0	33.0	37.0	26.0	37.0
7	32.949397872586864	37.0	35.0	37.0	26.0	37.0
8	32.92897947787964	37.0	35.0	37.0	26.0	37.0
9	34.31807428139732	39.0	35.0	39.0	26.0	39.0
10	33.82258980480561	39.0	34.0	39.0	22.0	39.0
11	34.192287479103605	39.0	35.0	39.0	26.0	39.0
12	34.032316159246534	39.0	35.0	39.0	25.0	39.0
13	34.123306894817	39.0	35.0	39.0	25.0	39.0
14	34.43230042752256	40.0	34.0	41.0	17.0	41.0
15	34.97931278297654	40.0	34.0	41.0	24.0	41.0
16	34.57221250274441	39.0	34.0	41.0	17.0	41.0
17	34.105679152353964	39.0	33.0	41.0	16.0	41.0
18	33.523886721214836	39.0	32.0	41.0	10.0	41.0
19	34.4059856616573	39.0	34.0	41.0	18.0	41.0
20	34.659282408647954	39.0	34.0	41.0	18.0	41.0
21	34.6805444386627	40.0	34.0	41.0	18.0	41.0
22	34.27417715304201	40.0	34.0	41.0	11.0	41.0
23	32.590807486917605	39.0	31.0	41.0	7.0	41.0
24	33.695099308668524	39.0	33.0	41.0	2.0	41.0
25	33.43451626677546	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6954160160164875
1101	2	1.076007314771804
1101	3	0.9049057300685988
1101	4	0.8356820405636896
1101	5	1.0866067555979555
1101	6	0.7357157066267845
1101	7	0.7246422766526521
1101	8	0.6340631306957576
1101	9	0.8849564582943117
1101	10	1.1885332862182736
1101	11	0.8257468437997133
1101	12	0.9272715999983063
1101	13	0.7439148239325348
1101	14	-0.03413669860597679
1101	15	0.4995634772462836
1101	16	1.105212680719049
1101	17	1.7962669399215656
1101	18	-3.8445495555587286
1101	19	-0.5416608849975262
1101	20	0.38343351929616176
1101	21	0.6968110188415437
1101	22	1.3658539106771102
1101	23	3.172377653925494
1101	24	1.9961433073807981
1101	25	2.199411496793971
1102	1	1.2035897069487973
1102	2	1.4889503224346399
1102	3	1.2717260525462493
1102	4	1.726311031623716
1102	5	1.5635504018303905
1102	6	1.1544657066267874
1102	7	1.1221598373167652
1102	8	1.0403570323560416
1102	9	1.2431281536966097
1102	10	1.5629406138044786
1102	11	1.2647075717690583
1102	12	1.349144604468293
1102	13	1.2083943897052052
1102	14	2.1531098690823995
1102	15	1.6810609229678661
1102	16	1.7778459821238997
1102	17	1.5684532426035602
1102	18	1.8047101028065313
1102	19	1.6092271258581547
1102	20	1.5103093175081668
1102	21	1.5378231516640213
1102	22	1.8672647503961457
1102	23	3.4676442567351984
1102	24	2.3864869376489963
1102	25	2.57648285694723
1103	1	0.8571060280046261
1103	2	1.1239529659169847
1103	3	0.8559194967100261
1103	4	1.3560737056529675
1103	5	1.3014990595288012
1103	6	0.836776893452452
1103	7	0.7914347398995432
1103	8	0.7712671735375736
1103	9	0.8090629673800578
1103	10	1.0420478021183257
1103	11	0.778644108611374
1103	12	0.9401406098727492
1103	13	0.7176710546095535
1103	14	1.636703634380332
1103	15	1.2542285980741
1103	16	1.3345144111121314
1103	17	1.8060122314226064
1103	18	1.8035665519243906
1103	19	1.2552579860668942
1103	20	1.1989364690090767
1103	21	1.0763499487424824
1103	22	1.5597783104815193
1103	23	3.0966966172670283
1103	24	2.0459980892282346
1103	25	2.1542164135079602
1104	1	0.6600588956934388
1104	2	1.0322437098125015
1104	3	0.8783499339615908
1104	4	1.3827630866864737
1104	5	1.2149005359626486
1104	6	0.7197979042417799
1104	7	0.7374881186616093
1104	8	0.7031240730265012
1104	9	0.8454059543192969
1104	10	1.1317695511245773
1104	11	0.8788712522797439
1104	12	0.877392171485468
1104	13	0.6739458984482809
1104	14	1.5532283362542714
1104	15	1.004654492452289
1104	16	1.3092446780059461
1104	17	1.7401405675952333
1104	18	2.3660310607262076
1104	19	1.4858088094626893
1104	20	1.0720199443072005
1104	21	1.0539195114909248
1104	22	1.441095743758062
1104	23	2.907315583763335
1104	24	1.9713246082515141
1104	25	2.019065930827665
1105	1	0.6688742027099686
1105	2	1.0554017969236398
1105	3	0.7332755662509349
1105	4	0.936444567796805
1105	5	1.0680960927858223
1105	6	0.6497899901863633
1105	7	0.4682896287558833
1105	8	0.5299933510745092
1105	9	0.6031290638750022
1105	10	1.1411515931564296
1105	11	0.6832914642119832
1105	12	0.7246113959594283
1105	13	0.5699790102908366
1105	14	1.3346586026341782
1105	15	0.707907284854798
1105	16	1.183704280350355
1105	17	1.5886956101075853
1105	18	1.4708950250512913
1105	19	1.048262076708518
1105	20	0.8751640790414257
1105	21	0.7476426762908517
1105	22	1.2103200892425576
1105	23	2.705209269477699
1105	24	1.6848648697236825
1105	25	1.7548492167537475
1106	1	-0.6315296004860933
1106	2	-0.3156685418961409
1106	3	-0.4936043576226581
1106	4	-2.9888795031434263
1106	5	-0.686656600537237
1106	6	-0.6638695446198568
1106	7	-0.7546056349528527
1106	8	-0.5376134185519916
1106	9	-0.5824300252116359
1106	10	-0.38249243779400643
1106	11	-0.4683212101575833
1106	12	-0.3747714438366714
1106	13	-0.4448105095712762
1106	14	0.2522600350811075
1106	15	-0.32446531276351465
1106	16	-1.185455738707283
1106	17	-5.778241994357938
1106	18	-7.030581486151579
1106	19	-3.0279261617098
1106	20	-1.6260774660850785
1106	21	-1.1572064767898738
1106	22	-1.4676449092734458
1106	23	-5.012094973189541
1106	24	-2.1743601848196867
1106	25	-0.6978822476121138
1107	1	0.48423367983415133
1107	2	0.7363111126764075
1107	3	0.5127937631078403
1107	4	0.8126315396906065
1107	5	0.8783265538701741
1107	6	0.36616959413712635
1107	7	0.34952452552683866
1107	8	0.44121981436453694
1107	9	0.3248150707839699
1107	10	0.7599227782226805
1107	11	0.45158258959513375
1107	12	0.4477681938315925
1107	13	0.32952850833654423
1107	14	1.1125144306560415
1107	15	0.5378124892856206
1107	16	-5.7097328091256365
1107	17	-1.2021864995600993
1107	18	0.4548075520141026
1107	19	0.3582269642350795
1107	20	0.20736112245763394
1107	21	0.34025486591442444
1107	22	0.4249197421723778
1107	23	2.2419283615810386
1107	24	1.2149385410449867
1107	25	0.9135993586632836
1108	1	0.40481951499285174
1108	2	0.5677629680381351
1108	3	0.3559965429291978
1108	4	0.8737310820675006
1108	5	0.6240887256333139
1108	6	0.3479609606748042
1108	7	0.3147164903135504
1108	8	0.34288210985079104
1108	9	0.3645200749888957
1108	10	0.8017163892333485
1108	11	0.27425675446414743
1108	12	0.3361548472530984
1108	13	0.18990635566527203
1108	14	0.7562722326242479
1108	15	0.560411699840742
1108	16	0.7072535563937166
1108	17	0.895469550195152
1108	18	0.6107055975214877
1108	19	0.6062938772395796
1108	20	0.5397765771781664
1108	21	0.6066495634162692
1108	22	1.0222682160000218
1108	23	2.6879474008080493
1108	24	1.3896603718699154
1108	25	1.590769327614744
1109	1	0.48054794566772685
1109	2	0.825763709294975
1109	3	0.6358085882442239
1109	4	0.7855131407664189
1109	5	0.47787558155783927
1109	6	0.4409665865008279
1109	7	0.30087455053531187
1109	8	0.2295074349263544
1109	9	0.3725521156528373
1109	10	0.8814116609983031
1109	11	0.4455187325959855
1109	12	0.5384701035692601
1109	13	0.3961053758348143
1109	14	-0.34528352244078775
1109	15	0.23676349679904263
1109	16	0.0352770054285898
1109	17	1.087792267172965
1109	18	1.8279355837032085
1109	19	0.9472122941520311
1109	20	0.7616690502564083
1109	21	0.7053582747665459
1109	22	0.656815407826528
1109	23	-3.948495363785188
1109	24	-1.0432819477054878
1109	25	0.15771926361191646
1110	1	0.5706187735185146
1110	2	0.9638275026604219
1110	3	0.6563017999523524
1110	4	1.1345634950108376
1110	5	0.8994911500288794
1110	6	0.4917055011658533
1110	7	0.3560375861957681
1110	8	0.361899201255369
1110	9	0.46394528973524274
1110	10	0.8363413931390582
1110	11	0.46826002303734526
1110	12	0.5593871210850878
1110	13	0.42441969011258607
1110	14	1.250469360464045
1110	15	0.7924973783006237
1110	16	1.1767142689861458
1110	17	1.4410991580886403
1110	18	2.0159638433328624
1110	19	1.1939876855620852
1110	20	0.7181101210804286
1110	21	0.638940648471177
1110	22	0.9127479191929311
1110	23	2.276958283825465
1110	24	1.3450910804275722
1110	25	1.6479068671643375
1111	1	0.5202896959720213
1111	2	0.8422386509852338
1111	3	0.59330678673577
1111	4	1.1195187592742144
1111	5	0.8344995793562262
1111	6	0.4293406497827661
1111	7	0.31812195175749736
1111	8	0.04777027662067468
1111	9	0.2867463343743424
1111	10	0.5147945129122249
1111	11	0.31269450940529
1111	12	0.4156830780691436
1111	13	0.34140547624924267
1111	14	-0.3340324056192614
1111	15	0.17353712688500877
1111	16	0.6132351088429431
1111	17	1.1579374909295979
1111	18	1.7595265554260635
1111	19	0.9236059187263876
1111	20	0.646317848031444
1111	21	0.5362373995196279
1111	22	0.7784520455616573
1111	23	0.5244729876630458
1111	24	0.537943320644402
1111	25	0.1947109700393881
1112	1	0.3981136053514227
1112	2	0.9319044542996942
1112	3	0.6293058046187916
1112	4	1.1249912923052818
1112	5	0.9332189505059887
1112	6	0.24375303184103814
1112	7	0.24380449536663207
1112	8	0.29322826250550804
1112	9	0.36577019870928495
1112	10	0.7402029129661258
1112	11	0.44574477644142263
1112	12	0.49998489812389835
1112	13	0.42369543336469917
1112	14	0.7876913942873216
1112	15	0.47294028775097274
1112	16	0.8203296117569892
1112	17	1.0265870985443257
1112	18	1.144075196984744
1112	19	0.5746627098253043
1112	20	0.5158775753278348
1112	21	0.6331169628098721
1112	22	0.6145167840545511
1112	23	2.189181204769973
1112	24	1.1790098406088845
1112	25	1.2123432927645013
1113	1	0.4347413285902064
1113	2	0.8436669927618965
1113	3	0.5537781793490915
1113	4	0.6531065510614482
1113	5	0.7360044614247983
1113	6	0.2652406535482328
1113	7	0.1348584243289892
1113	8	0.11568099457874581
1113	9	0.2821665166807179
1113	10	0.7265342150930039
1113	11	0.34580611485420576
1113	12	0.4332521526050499
1113	13	0.29461958920736464
1113	14	1.0704372722597952
1113	15	0.44885871197145377
1113	16	0.8732409597943018
1113	17	1.3203402767934662
1113	18	1.797570121841602
1113	19	0.8129499459767544
1113	20	0.7714009054721487
1113	21	0.6154386432812728
1113	22	0.7673471136731038
1113	23	0.6837526834135801
1113	24	0.587211734486246
1113	25	0.9979009695469756
1114	1	-0.5943103814685315
1114	2	-3.2708640011727894
1114	3	-1.239569261335273
1114	4	-0.02085961382279322
1114	5	0.08468214365178994
1114	6	-0.06591448789134091
1114	7	-0.07288879221462707
1114	8	-0.15942192431946722
1114	9	-0.07546158989313767
1114	10	0.05672929638626556
1114	11	-0.2120607748268739
1114	12	-0.036244431782812114
1114	13	-0.08917413372538618
1114	14	0.6486447939276729
1114	15	0.021556060014731315
1114	16	0.3424790502052417
1114	17	0.6938290162551937
1114	18	0.8082467959984001
1114	19	0.14676028235682992
1114	20	0.017294286562098193
1114	21	-0.2086180508116655
1114	22	-6.176545552278867E-4
1114	23	1.5684621170358142
1114	24	0.48279551412998956
1114	25	0.423631398061282
1115	1	-0.24989884262337725
1115	2	0.09946969461812216
1115	3	-0.13504574890511734
1115	4	-0.0714313737972958
1115	5	-0.05297526907067862
1115	6	-0.4815278072657563
1115	7	-0.6005143370343973
1115	8	-0.5699395876747317
1115	9	-0.5561228157437341
1115	10	-0.31958188053604886
1115	11	-0.3579193773122782
1115	12	-0.5187691871239437
1115	13	-0.6072449573317655
1115	14	0.24523118286673906
1115	15	-0.5149310494918637
1115	16	0.006026396255514044
1115	17	0.4608660685334769
1115	18	0.8109526034336838
1115	19	0.13391679916507115
1115	20	-0.24258389142246983
1115	21	-0.258382127894464
1115	22	0.05212871139487163
1115	23	1.689197038719172
1115	24	0.42540260011752906
1115	25	0.2044655294290223
1116	1	0.3180147558351436
1116	2	0.20170608148457347
1116	3	0.2901699167137508
1116	4	1.0948836695785928
1116	5	0.5110703140734429
1116	6	0.3178820275119847
1116	7	0.2473822105699739
1116	8	0.2359942241335915
1116	9	0.25303002330122837
1116	10	0.6831628678226949
1116	11	0.33881450170657956
1116	12	0.4329685897785396
1116	13	0.2783584999737201
1116	14	0.8762110518934847
1116	15	0.28626433785793637
1116	16	0.6308173180327117
1116	17	0.904446112351259
1116	18	1.4243975879021562
1116	19	0.5915958481360164
1116	20	0.4580694882526686
1116	21	0.32718023660702755
1116	22	0.7193710364647288
1116	23	2.0433483393412004
1116	24	0.9983728774591754
1116	25	1.239385275032788
1201	1	1.0075465372584773
1201	2	1.353093586373138
1201	3	1.034471106193923
1201	4	1.5996607661524251
1201	5	1.4487899967980127
1201	6	0.9228296790030157
1201	7	0.8591929985884761
1201	8	0.9238308994493352
1201	9	1.0891354352907072
1201	10	1.3692405678542983
1201	11	1.1218401631914716
1201	12	1.197437152824989
1201	13	1.1194950474300356
1201	14	1.9780468972126357
1201	15	1.4368176083835493
1201	16	1.7672573167250576
1201	17	2.1442505788347717
1201	18	2.601974557827347
1201	19	1.9072665289753843
1201	20	1.6192045343292847
1201	21	1.551033073548716
1201	22	1.804911614883359
1201	23	3.412975534049558
1201	24	2.2836459923498396
1201	25	2.3917925678635825
1202	1	1.2425803678683387
1202	2	1.5543059258829146
1202	3	1.3204149893278583
1202	4	1.9083093553785062
1202	5	1.666178514857215
1202	6	1.2964795955156774
1202	7	1.237373758134133
1202	8	1.241123315880941
1202	9	1.3129078471831974
1202	10	1.7947254158478998
1202	11	1.4270199919478586
1202	12	1.4492623407390468
1202	13	1.4765537128214277
1202	14	2.327263764356985
1202	15	1.8522833846536955
1202	16	2.076856199237568
1202	17	2.3714984214030466
1202	18	3.031029148144974
1202	19	2.247980318706176
1202	20	1.9792089822591237
1202	21	1.8799707410637652
1202	22	2.2373816884548887
1202	23	3.867690153925494
1202	24	2.532601640714134
1202	25	2.769723996793971
1203	1	1.0758927240248397
1203	2	1.4337560841107617
1203	3	1.187638450202428
1203	4	1.8554818317242407
1203	5	1.524217967932266
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2211	17	0.09816947726415748
2211	18	-5.57798105094037
2211	19	-1.760489699526829
2211	20	-1.026310930658255
2211	21	-0.7671343472711172
2211	22	-0.3835685596191212
2211	23	-4.800447221599207
2211	24	-2.205851600277061
2211	25	-1.5778717922091268
2212	1	0.2584653583211498
2212	2	0.4797483358665495
2212	3	0.2812501980021622
2212	4	0.8081732510868136
2212	5	0.6961086726131711
2212	6	0.15945752332073226
2212	7	0.08291006776618559
2212	8	-0.007330948530771764
2212	9	-0.06258087803956869
2212	10	-3.5071287091445207
2212	11	-1.0952558385061621
2212	12	-0.3359490802768974
2212	13	-0.1622764532693779
2212	14	0.625003504915874
2212	15	0.31123756950548653
2212	16	0.44841617984010185
2212	17	1.0151902668901016
2212	18	1.4820207110048855
2212	19	0.6589091997148415
2212	20	0.2956997646741115
2212	21	0.21155991257980133
2212	22	0.4944573120531217
2212	23	1.6256286237988036
2212	24	0.8810215238747077
2212	25	0.8350014904291783
2213	1	-1.6072034027008542
2213	2	-3.849252797478293
2213	3	-1.836684332900358
2213	4	-3.4773225103859673
2213	5	-1.3250161187938367
2213	6	-1.146177221167477
2213	7	-1.0185503444145994
2213	8	-1.0391047811127336
2213	9	-0.8418238166360013
2213	10	-0.8899970561520192
2213	11	-0.9783578155534656
2213	12	-0.7964309453613225
2213	13	-1.2358575536514138
2213	14	-6.280707016647916
2213	15	-2.883109069187391
2213	16	-2.052937034784158
2213	17	-0.9893718103406997
2213	18	-0.241587519895468
2213	19	-0.8440992929394611
2213	20	-1.0003958101006774
2213	21	-1.132023193709287
2213	22	-0.9734327960289164
2213	23	-5.624937423155906
2213	24	-3.0419225796047833
2213	25	-2.191635939737516
2214	1	0.039782441265266755
2214	2	0.09874236599950237
2214	3	-0.01346070622063067
2214	4	0.27034096419320264
2214	5	-0.09930433932049709
2214	6	-0.14052634424761834
2214	7	-0.3165514906440592
2214	8	-0.2996380337039355
2214	9	-0.2874609039208451
2214	10	0.23753219321350372
2214	11	-0.28601013376609075
2214	12	-0.17247290553664385
2214	13	-0.22332079403837923
2214	14	0.39119166284370976
2214	15	0.06788547571534309
2214	16	0.3796222005329781
2214	17	0.6349024504320724
2214	18	1.1968157987661812
2214	19	0.20494647603997862
2214	20	0.1781455106993306
2214	21	0.0017446289222107225
2214	22	0.4094558766136345
2214	23	1.9725877905326854
2214	24	0.9944858594917321
2214	25	0.8532298371931901
2215	1	-2.499708304107834
2215	2	-4.483672010532523
2215	3	-2.490063594588225
2215	4	-4.098289080907435
2215	5	-4.93520996934377
2215	6	-2.4361714340103546
2215	7	-1.8044385961782226
2215	8	-2.082562257804632
2215	9	-1.7373867656114115
2215	10	-3.2983689897465034
2215	11	-2.0247857254828574
2215	12	-1.8069961577069549
2215	13	-1.700966683448975
2215	14	-0.8999927753995536
2215	15	-1.540856750673946
2215	16	-2.3524043550229905
2215	17	-6.742870427340801
2215	18	-3.002695518392194
2215	19	-2.1331466561708012
2215	20	-2.056276076663437
2215	21	-2.028244018713494
2215	22	-1.7704834209727167
2215	23	-6.497809797511959
2215	24	-3.7829476826476913
2215	25	-3.4303112110537377
2216	1	-1.10556812898783
2216	2	-0.6304370823696388
2216	3	-0.9833023443111237
2216	4	-3.560774722067425
2216	5	-1.4984429792626592
2216	6	-1.1877707792696413
2216	7	-1.3356869401066795
2216	8	-1.0953174607615637
2216	9	-1.195386552141457
2216	10	-0.7986246312125189
2216	11	-1.366893718887681
2216	12	-1.9926194787769589
2216	13	-1.329982773560161
2216	14	-7.167016817639734
2216	15	-3.5508293802431155
2216	16	-2.0148449137171482
2216	17	-1.171200875844164
2216	18	-0.3025999047519079
2216	19	-0.7723548169927952
2216	20	-1.3355044798111493
2216	21	-1.5325824596513584
2216	22	-0.9757930009220672
2216	23	-0.01674853835271506
2216	24	-0.6946599430631224
2216	25	-0.7533196147537851
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107288.0
3	2.0
4	922.0
5	5553.0
6	6607.0
7	5796.0
8	5556.0
9	5159.0
10	4889.0
11	4726.0
12	4720.0
13	4738.0
14	4738.0
15	4828.0
16	5146.0
17	5344.0
18	6253.0
19	7182.0
20	8924.0
21	11103.0
22	13620.0
23	16685.0
24	19826.0
25	23129.0
26	26559.0
27	30362.0
28	34191.0
29	41491.0
30	49009.0
31	55341.0
32	71107.0
33	93882.0
34	122697.0
35	161264.0
36	228340.0
37	318447.0
38	798372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.722570528846276	17.770257816130446	11.737154408436895	25.77001724658638
2	14.09237727494822	19.45483398120803	41.700660270075105	24.752128473768643
3	19.622549002652907	23.650546318618414	28.633779750066584	28.0931249286621
4	10.752952681439837	15.88557529894292	39.01893027741954	34.342541742197696
5	12.570055940990233	37.58833328361377	36.338513246804006	13.50309752859199
6	33.198765067063675	35.937119054659014	17.81142632852121	13.052689549756108
7	29.20811510403248	30.641728151277213	21.327350003198873	18.822806741491434
8	25.733299210978178	36.713031569518826	20.043886420974182	17.50978279852882
9	26.68166824464245	15.02423165549114	19.337293404061235	38.95680669580518
10	13.862848006613277	29.228469776123113	35.10765382159285	21.80102839567076
11	35.28119758615323	22.301713554544982	23.20595507755088	19.211133781750902
12	23.166398867766134	24.602527208930262	31.67047291254107	20.560601010762532
13	29.465128623645725	21.09512513097515	25.181981969520393	24.257764275858733
14	20.614674603994573	21.492947919607104	28.09847796794953	29.793899508448796
15	24.153278726792653	29.69654291143815	22.70654258289975	23.44363577886945
16	22.31868470572266	27.80747830890983	27.83670092254408	22.037136062823432
17	22.16596459919902	27.920151065551657	28.018414572568872	21.895469762680445
18	21.784718358779102	27.020499699353884	29.77516165942825	21.419620282438764
19	23.53934789676473	26.976603626100733	27.364280625056203	22.119767852078333
20	23.530492499484488	27.10750846971846	27.743066978321757	21.618932052475294
21	23.11668975248886	26.597850165232234	27.07361696903041	23.211843113248495
22	22.888771576202586	27.50970964245428	26.84647502039641	22.755043760946723
23	21.330731346277556	26.897407120475343	29.012011565411672	22.75984996783543
24	22.916471200163606	27.30872782977487	27.43722768903899	22.337573281022532
25	22.826658850191283	26.658115235080064	27.4520408652942	23.063185049434452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	170.0
2	305.5
3	441.0
4	441.0
5	441.0
6	769.5
7	1098.0
8	1098.0
9	1098.0
10	1899.0
11	2700.0
12	2700.0
13	2700.0
14	5127.5
15	7555.0
16	7555.0
17	7555.0
18	14398.5
19	21242.0
20	21242.0
21	21242.0
22	35012.5
23	48783.0
24	48783.0
25	48783.0
26	70596.5
27	92410.0
28	92410.0
29	92410.0
30	121671.0
31	150932.0
32	150932.0
33	150932.0
34	177221.5
35	203511.0
36	203511.0
37	203511.0
38	228015.5
39	252520.0
40	252520.0
41	252520.0
42	272660.0
43	292800.0
44	292800.0
45	292800.0
46	313573.5
47	334347.0
48	334347.0
49	334347.0
50	320014.0
51	305681.0
52	305681.0
53	305681.0
54	269650.0
55	233619.0
56	233619.0
57	233619.0
58	199341.5
59	165064.0
60	165064.0
61	165064.0
62	134023.5
63	102983.0
64	102983.0
65	102983.0
66	79392.0
67	55801.0
68	55801.0
69	55801.0
70	40724.0
71	25647.0
72	25647.0
73	25647.0
74	18086.5
75	10526.0
76	10526.0
77	10526.0
78	7234.0
79	3942.0
80	3942.0
81	3942.0
82	2681.0
83	1420.0
84	1420.0
85	1420.0
86	936.5
87	453.0
88	453.0
89	453.0
90	289.0
91	125.0
92	125.0
93	125.0
94	71.5
95	18.0
96	18.0
97	18.0
98	13.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11297452325096939
2	0.04780023822324872
3	0.03716835883543752
4	0.03150666696631855
5	0.03556925502507567
6	0.020399378337588966
7	0.02074513051280234
8	0.02385690008972269
9	0.030512629462580106
10	0.03773020612015925
11	0.02057225442519565
12	0.02130697779752407
13	0.016423228322635184
14	0.02191204410414747
15	0.0224738913888692
16	0.022430672366967528
17	0.027141545754249728
18	0.019232464746243835
19	0.03405658925851717
20	0.023986557155427704
21	0.029734687068350016
22	0.038421710470586
23	0.029907563155956707
24	0.041187727872292974
25	0.03167954305392524
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2313796.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.27507444260644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.41340287112146	65.9118873683373
2	9.526418072776984	14.532564953449118
3	2.2291554035145653	5.100869830416357
4	0.7551135159556225	2.303853585685336
5	0.3493171148475771	1.3322094469537724
6	0.18091660900576387	0.8279656691891137
7	0.13080621201778217	0.6984077491448187
8	0.08462342034015213	0.5163726148826464
9	0.05345791201031837	0.3669755596320005
>10	0.24932920022969954	3.2585813523068543
>50	0.015410603539431168	0.804157023186389
>100	0.009355790749701215	1.4544918939782532
>500	0.001489896195027468	0.7491626562430999
>1k	0.0010887702963628615	1.4699585351254614
>5k	1.1460739961714331E-4	0.672541761469343
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8414	0.3636448502806643	No Hit
CCCATGTACTCTGCGTTGATACCAC	6973	0.30136623972035564	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3408	0.1472904266408966	No Hit
GCGTTGATACCACTGCTTCCCATGT	2370	0.10242908190696155	No Hit
GTCCTACAGTGGACATTTCTAAATT	2314	0.10000881668046793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.321902190167154E-5
2	0.0	0.0	0.0	0.0	4.321902190167154E-5
3	0.0	0.0	0.0	0.0	4.321902190167154E-5
4	0.0	0.0	0.0	0.0	4.321902190167154E-5
5	0.0	0.0	0.0	0.0	4.321902190167154E-5
6	0.0	0.0	0.0	0.0	8.643804380334307E-5
7	0.0	0.0	0.0	0.0	8.643804380334307E-5
8	0.0	0.0	0.0	0.0	8.643804380334307E-5
9	0.0	0.0	0.0	0.0	8.643804380334307E-5
10	0.0	0.0	0.0	0.0	1.2965706570501463E-4
11	4.321902190167154E-5	0.0	0.0	0.0	1.2965706570501463E-4
12	4.321902190167154E-5	0.0	0.0	0.0	1.2965706570501463E-4
13	4.321902190167154E-5	0.0	0.0	0.0	1.2965706570501463E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	775	0.0	12.872592	4
GCCGGTT	115	5.338734E-9	12.389654	11
CGAAATC	120	1.00426405E-8	11.87342	13
CCGGTTT	105	2.7265924E-7	11.760339	12
GTAGGAC	1515	0.0	11.539162	3
GTCCTAC	1650	0.0	11.357364	1
TGTAGGA	1690	0.0	11.187804	2
AGGACGT	1080	0.0	10.996522	5
GACGTGA	580	0.0	10.808672	7
TGCCGGT	115	8.839288E-7	10.737469	10
GGCGAGG	540	0.0	10.730053	19
CTGTAGG	1625	0.0	10.712554	1
AAGTCGT	80	3.7775715E-4	10.685846	7
GGACGTG	1085	0.0	10.681298	6
TGGCGAG	1285	0.0	10.644497	18
TTACTCT	340	0.0	10.618988	4
GTCCTAA	720	0.0	10.569458	1
TCCTACA	1945	0.0	10.453763	2
ATAGGAC	255	0.0	10.432464	3
TCCAACG	165	9.313226E-10	10.362256	18
>>END_MODULE