FastQCFastQC Report
Thu 26 May 2016
SRR1513101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313796
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC98000.4235464146363811No Hit
CCCATGTACTCTGCGTTGATACCAC92150.3982632868239033No Hit
GAGTACATGGGAAGCAGTGGTATCA35610.15390293699185234No Hit
GTATCAACGCAGAGTACTTTTTTTT26810.11587019771838139No Hit
CATGTACTCTGCGTTGATACCACTG25100.10847974497319557No Hit
GTATCAACGCAGAGTACATGGGAAG24660.10657810800952201No Hit
GCGTTGATACCACTGCTTCCCATGT24000.1037256525640117No Hit
GTCCTACAGTGGACATTTCTAAATT23800.10286127212597827No Hit
ACGCAGAGTACATGGGAAGCAGTGG23440.10130538733751808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1351.8189894E-1213.35706918
TAGGACC8650.012.8767524
TGGACCG701.0701352E-412.2399885
TATGTCG701.09886496E-412.20530216
GTCCTAC15700.011.8956691
GGACCGT657.885711E-411.7169116
ATGTCGA658.077172E-411.683454517
AATCGAA752.100231E-411.3849667
ATCCCGT1001.9697964E-611.38102810
GTAGGAC16400.011.3195023
AGGACCT12800.010.9331155
ATCGAAC803.8192116E-410.6731758
TGTAGGA18550.010.6300742
ATACCGT909.318024E-510.5777676
TCGAACC1002.4361241E-510.4357679
TCCTACA19850.010.3658062
GGACCTG12400.010.2877326
GAATCGA750.002602617710.1546576
CGGAATC856.4692786E-410.079994
GGAATCG856.4692786E-410.079995
Produced by FastQC (version 0.11.5)