FastQCFastQC Report
Thu 26 May 2016
SRR1513100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238555
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC101760.4545789583012255No Hit
CCCATGTACTCTGCGTTGATACCAC83360.37238307747631844No Hit
GAGTACATGGGAAGCAGTGGTATCA40360.18029487772245933No Hit
GCGTTGATACCACTGCTTCCCATGT27850.12441061309639476No Hit
GTATCAACGCAGAGTACATGGGAAG26950.12039016240387214No Hit
CATGTACTCTGCGTTGATACCACTG26460.11820125036016538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6300.013.2723864
TAGGCGG550.00306579312.0926875
CCAACGA658.0309936E-411.69141319
GTAGGAC12200.011.2921393
TGTAGGA13600.010.9685252
GTCCTAA5550.010.6250971
GCGTTTA1856.002665E-1110.26856713
CTGTAGG13900.010.2638591
GTATAGA2700.010.2157071
GACGTGA2700.010.2024677
TTATACT3450.010.1903594
ATAAGAC2450.010.0826643
GTATTAT2850.010.0117641
AGGACCG1151.0249927E-59.9145015
TCTACAC4800.09.8968463
GTCCTAC12300.09.8204821
TAAGACA4550.09.8150684
GTCTAAA2251.8189894E-129.7225361
CTTATAC2151.0913936E-119.7219253
TAGGACG6850.09.7098914
Produced by FastQC (version 0.11.5)