##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513100_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2238555
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.565415636426177	31.0	30.0	34.0	25.0	34.0
2	29.252964523989807	31.0	30.0	34.0	22.0	34.0
3	29.632731382521314	31.0	30.0	34.0	25.0	34.0
4	32.53694905865614	35.0	33.0	37.0	22.0	37.0
5	32.754549251637776	35.0	33.0	37.0	25.0	37.0
6	33.222039663979665	37.0	35.0	37.0	28.0	37.0
7	33.23788381344215	37.0	35.0	37.0	27.0	37.0
8	33.221602328287666	37.0	35.0	37.0	27.0	37.0
9	34.62700581401842	39.0	35.0	39.0	27.0	39.0
10	34.141342964546325	39.0	34.0	39.0	25.0	39.0
11	34.51566970657411	39.0	35.0	39.0	27.0	39.0
12	34.3654875578219	39.0	35.0	39.0	27.0	39.0
13	34.44861662992422	39.0	35.0	39.0	27.0	39.0
14	34.77595591799174	40.0	34.0	41.0	18.0	41.0
15	35.32739959482792	40.0	35.0	41.0	26.0	41.0
16	34.91258780775992	40.0	34.0	41.0	22.0	41.0
17	34.4451554685947	39.0	34.0	41.0	17.0	41.0
18	33.8483235837404	39.0	33.0	41.0	13.0	41.0
19	34.75856523516286	39.0	34.0	41.0	22.0	41.0
20	35.024925900860154	40.0	35.0	41.0	24.0	41.0
21	35.050919901454286	40.0	35.0	41.0	24.0	41.0
22	34.63402150047687	40.0	34.0	41.0	18.0	41.0
23	32.942059944919826	39.0	32.0	41.0	10.0	41.0
24	34.05228015393859	39.0	33.0	41.0	16.0	41.0
25	33.786939789283714	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8639819304664442
1101	2	1.2021473802503913
1101	3	1.0253283699079567
1101	4	1.104033681875194
1101	5	1.2951667508878444
1101	6	0.9014890836384168
1101	7	1.004563257348785
1101	8	0.9320950978713753
1101	9	1.0004524738834704
1101	10	1.3978612636494887
1101	11	1.1415320613904356
1101	12	1.183132569570958
1101	13	0.9789380508305925
1101	14	0.36253008850862756
1101	15	0.8563900991561724
1101	16	1.4967812928947808
1101	17	2.018247877088804
1101	18	-4.1336640632427475
1101	19	-0.47084428830667235
1101	20	0.521841178487513
1101	21	0.9582319322280597
1101	22	1.4701518516244931
1101	23	3.323701169478703
1101	24	2.2967998516825148
1101	25	2.521546458258406
1102	1	0.7802516395682133
1102	2	0.9869173526856478
1102	3	0.7762202453250957
1102	4	1.3431250306598983
1102	5	1.1385672182130335
1102	6	0.7930763408018962
1102	7	0.7364517725114652
1102	8	0.659596880413261
1102	9	0.7775864228503977
1102	10	1.1072644063634556
1102	11	0.7965417920787274
1102	12	0.9268172505393508
1102	13	0.7444718553116729
1102	14	1.667284779916237
1102	15	1.1085030492312669
1102	16	1.362060416460146
1102	17	0.9082413168510612
1102	18	1.3803357017043751
1102	19	1.0942409641286304
1102	20	1.0677849058614015
1102	21	1.0836845407062157
1102	22	1.4634953302844593
1102	23	2.9800548983266424
1102	24	1.8903558623567775
1102	25	2.102541647273476
1103	1	0.5882589847334927
1103	2	0.9090909394421196
1103	3	0.68488980511016
1103	4	1.0822953319606867
1103	5	0.9936103153954434
1103	6	0.6716193817996654
1103	7	0.6323718468592929
1103	8	0.5677025096283046
1103	9	0.6704558533847305
1103	10	0.9288028721770516
1103	11	0.7696494072950202
1103	12	0.8509044150662248
1103	13	0.7842619592072353
1103	14	1.5742859076037021
1103	15	1.0568142187211507
1103	16	1.220385148419446
1103	17	1.6791476923926965
1103	18	1.658489114786903
1103	19	1.1543003513842862
1103	20	1.03246577968806
1103	21	0.9794059280900456
1103	22	1.1968182603405708
1103	23	2.700115039342279
1103	24	1.6450035134305168
1103	25	1.7986177186764252
1104	1	0.6192319401199242
1104	2	1.054793736683017
1104	3	0.7875873650808742
1104	4	1.2918227219030314
1104	5	1.1111653150348104
1104	6	0.7108898274164801
1104	7	0.6094470496588684
1104	8	0.641935223341676
1104	9	0.8147111756249927
1104	10	1.0778505067917123
1104	11	0.8331800424457043
1104	12	0.877643914710518
1104	13	0.64751150555783
1104	14	1.5578175390447555
1104	15	1.0458384197559383
1104	16	1.3976272817469706
1104	17	1.7769312576489398
1104	18	2.4535366176006477
1104	19	1.5604578410231298
1104	20	1.1125797200216851
1104	21	1.189982017432385
1104	22	1.4559344469976452
1104	23	2.9352810799546063
1104	24	1.8171339506722646
1104	25	1.9794763341752812
1105	1	0.6578019271425788
1105	2	0.989321878090692
1105	3	0.7163046089077376
1105	4	0.7929028617119798
1105	5	0.9216890127598276
1105	6	0.6232979010092095
1105	7	0.4558959649764347
1105	8	0.41693483379143004
1105	9	0.6996773716042739
1105	10	1.0886725105989967
1105	11	0.6921382463014751
1105	12	0.6855496644976711
1105	13	0.784241682963696
1105	14	1.3951954256865733
1105	15	0.9070411142004744
1105	16	1.1791149685959113
1105	17	1.620685932784042
1105	18	1.6195093739466273
1105	19	1.0866406837317655
1105	20	0.8670795362305981
1105	21	0.7775438441650309
1105	22	1.1895356376334902
1105	23	2.6530836453324156
1105	24	1.6710518426766754
1105	25	1.6330501106916273
1106	1	-0.44545409007578485
1106	2	-0.00364070736985056
1106	3	-0.17140726780561621
1106	4	-2.6063772178877542
1106	5	-0.34787538235088533
1106	6	-0.3420554065175381
1106	7	-0.31276970418785766
1106	8	-0.2743624136008904
1106	9	-0.17647834673923768
1106	10	-0.02192357167000125
1106	11	-0.08768534338814504
1106	12	-0.10627940382395451
1106	13	-0.1924039994786142
1106	14	0.5475787194466903
1106	15	-0.009724699053819563
1106	16	-0.9101880314625177
1106	17	-5.872583147182318
1106	18	-7.042212862076006
1106	19	-2.6828989389690605
1106	20	-1.2933983560165885
1106	21	-0.8543164476437042
1106	22	-1.1978185767342993
1106	23	-4.824998324350936
1106	24	-1.9346507489957645
1106	25	-0.5539178590949163
1107	1	0.6594217200218253
1107	2	1.0492747991580735
1107	3	0.7802267324856906
1107	4	1.11106004337487
1107	5	1.1559085089361005
1107	6	0.6525775120102253
1107	7	0.6978603715423475
1107	8	0.6603515003250635
1107	9	0.5428271617386358
1107	10	0.9701102924968552
1107	11	0.6245441777059071
1107	12	0.7470779326518198
1107	13	0.7509842995639531
1107	14	1.556952820722877
1107	15	0.994100759529772
1107	16	-5.3826020471438625
1107	17	-0.7113785809978879
1107	18	1.0762959229862616
1107	19	1.012365211062523
1107	20	0.7483424756064565
1107	21	0.8348556433298739
1107	22	0.9443079520888986
1107	23	2.8376992005700146
1107	24	1.8028807517492353
1107	25	1.6312828653250548
1108	1	0.5312120209134079
1108	2	0.7679501521527428
1108	3	0.5500213857427774
1108	4	0.9903034770990757
1108	5	0.752926906589451
1108	6	0.4047451930731114
1108	7	0.36007129439499863
1108	8	0.3213785890734613
1108	9	0.5769709394631803
1108	10	0.9765285058818307
1108	11	0.5401228707873145
1108	12	0.5683233065070112
1108	13	0.440464148401027
1108	14	1.0348118885902906
1108	15	0.7603828228466938
1108	16	0.943650758725056
1108	17	1.0485126239993932
1108	18	0.8659937559412683
1108	19	0.798223440484719
1108	20	0.5867039720465996
1108	21	0.7220228167858451
1108	22	1.0767655020578317
1108	23	2.7447109357267507
1108	24	1.5559160623161148
1108	25	1.7397197578129138
1109	1	0.24412733445267776
1109	2	0.593176688290697
1109	3	0.40131385493397076
1109	4	0.27152619220145624
1109	5	0.21266181614066682
1109	6	0.18960320801190989
1109	7	0.1627898176008955
1109	8	0.10887142198594546
1109	9	0.19116764643324302
1109	10	0.6440244909695707
1109	11	0.2845726366984067
1109	12	0.3503702519376688
1109	13	0.20715547400062206
1109	14	-0.8746823378897517
1109	15	-0.01179729303319732
1109	16	-0.21985400425896273
1109	17	0.7472486033526451
1109	18	1.5511341071141018
1109	19	0.7207739573087579
1109	20	0.5151574906093046
1109	21	0.5365440870896805
1109	22	0.3511603222653932
1109	23	-4.150886284660366
1109	24	-1.2452752189560172
1109	25	0.010572695567795165
1110	1	0.6700916172469391
1110	2	1.0310442785712617
1110	3	0.7546248340793547
1110	4	1.3720904349611729
1110	5	0.8887781080394959
1110	6	0.6866615459893168
1110	7	0.5829170604204421
1110	8	0.5296045867230958
1110	9	0.6215141144182894
1110	10	0.988976457345565
1110	11	0.6614435390701914
1110	12	0.7630614223780157
1110	13	0.5409959742382284
1110	14	1.492365220833065
1110	15	0.9310474402781423
1110	16	1.3292466594436405
1110	17	1.5910244374605114
1110	18	2.2613511081381503
1110	19	1.5437171824955627
1110	20	1.0460801835246016
1110	21	0.9688512454106473
1110	22	1.2015091209000843
1110	23	2.6441215671855005
1110	24	1.7771576754069756
1110	25	2.026906430186365
1111	1	0.39216761745834106
1111	2	0.7300321756168735
1111	3	0.5081420900383407
1111	4	0.954892612435934
1111	5	0.7346002104653735
1111	6	0.29315168921641543
1111	7	0.1748334496176298
1111	8	0.046643855260697364
1111	9	0.18007867231692387
1111	10	0.42144831401275695
1111	11	0.3092158555439468
1111	12	0.3008554078967478
1111	13	0.16847866542791223
1111	14	-0.5075396467743545
1111	15	-0.007676728634706365
1111	16	0.3503158418335772
1111	17	0.8079738587384568
1111	18	1.4423854086508072
1111	19	0.5512141435441364
1111	20	0.39042509129902925
1111	21	0.21627474311161166
1111	22	0.5795652490117078
1111	23	0.7070859982409416
1111	24	0.5128085395262119
1111	25	-0.05163328758753494
1112	1	0.13899792233050334
1112	2	0.569116404766902
1112	3	0.2826026564347899
1112	4	0.8215976796604707
1112	5	0.6439162329214554
1112	6	0.023897343239170254
1112	7	-0.11665552612405605
1112	8	0.005104817442038723
1112	9	0.03246904814766083
1112	10	0.42081246673861017
1112	11	0.10919371043695492
1112	12	0.03313684864798461
1112	13	-0.08190192415022324
1112	14	0.26843809503725424
1112	15	0.04532145970707546
1112	16	0.4332641094501284
1112	17	0.6941169114178933
1112	18	0.6890694144650737
1112	19	0.20637878299116608
1112	20	0.12811088050116837
1112	21	0.2509977997017856
1112	22	0.10094210925959146
1112	23	1.7320432982341032
1112	24	0.758894863037952
1112	25	0.8099807608914062
1113	1	0.6270995080227877
1113	2	1.0762353144066346
1113	3	0.7233870921487728
1113	4	0.8759923399981915
1113	5	1.0423243960300113
1113	6	0.61976540319516
1113	7	0.4935817708639334
1113	8	0.4090712371723697
1113	9	0.6417117639097256
1113	10	0.9696168416978068
1113	11	0.6754550635559227
1113	12	0.581783998511078
1113	13	0.5124608610458878
1113	14	1.2967518232383028
1113	15	0.7948289311634653
1113	16	1.1781589790884794
1113	17	1.6731079671763496
1113	18	2.2038696187732114
1113	19	1.1350697131692442
1113	20	1.0245788075800633
1113	21	0.887272786164452
1113	22	1.1044875466979036
1113	23	1.0391576762272052
1113	24	0.8520602950229303
1113	25	1.3134639841978952
1114	1	-0.45600476624924013
1114	2	-3.232646338411687
1114	3	-1.1926844700939085
1114	4	0.01961686279181407
1114	5	0.17123748832733554
1114	6	0.06607725288450439
1114	7	-0.01581264488574874
1114	8	0.02317184350517465
1114	9	0.019889022686577107
1114	10	0.16025760822224555
1114	11	-0.0977869455765017
1114	12	-0.05582136117153169
1114	13	-0.06506589993921352
1114	14	0.6306083993293328
1114	15	0.11299645867300967
1114	16	0.4720934053179917
1114	17	0.8407148239770095
1114	18	1.0706216211685984
1114	19	0.4172884421160248
1114	20	0.2377594348725225
1114	21	0.21853859947383114
1114	22	0.400183332310597
1114	23	1.9290311697521645
1114	24	0.9686289004574675
1114	25	0.7854379343438609
1115	1	0.11074495874530754
1115	2	0.40878863337520244
1115	3	0.17575113854772084
1115	4	0.30048672620205963
1115	5	0.2935191651703164
1115	6	-0.1271364586425605
1115	7	-0.23526610772772472
1115	8	-0.15213890152579523
1115	9	-0.3669545827128786
1115	10	0.006864909338744951
1115	11	-0.14915885538361806
1115	12	-0.24024001967933373
1115	13	-0.3438875850657652
1115	14	0.5965716309885849
1115	15	-0.15971364772181573
1115	16	0.44990035371494486
1115	17	0.8638624896594465
1115	18	1.2011171654121924
1115	19	0.3099824267335336
1115	20	0.031546315664897406
1115	21	0.10361125880998401
1115	22	0.2941002561686048
1115	23	2.0483412289395773
1115	24	0.834126960945305
1115	25	0.503932997606249
1116	1	0.05833081576193422
1116	2	0.020069063019775513
1116	3	0.04340420142775514
1116	4	0.6170972706831961
1116	5	0.11625329165967457
1116	6	-0.008390492968700869
1116	7	-0.03615699510130099
1116	8	-0.20283659686015199
1116	9	-0.08937116379423315
1116	10	0.2840064924518444
1116	11	-0.04843688649096123
1116	12	-0.04471737807610765
1116	13	-0.0900947721281824
1116	14	0.5812229485282856
1116	15	-0.20450612305950244
1116	16	0.2386880219027745
1116	17	0.5027643246930396
1116	18	1.0759260569799523
1116	19	0.3212068638392367
1116	20	0.10085638134982133
1116	21	-0.13597894811126565
1116	22	0.2421529893164447
1116	23	1.6578903941743874
1116	24	0.5352045172791904
1116	25	0.8120519083343041
1201	1	0.9999395821134485
1201	2	1.2922665790364896
1201	3	1.1040482088008545
1201	4	1.6654502976199623
1201	5	1.4367004264804564
1201	6	0.961284261710496
1201	7	0.8601290240138084
1201	8	0.8762824566150584
1201	9	1.0960446426575103
1201	10	1.336262203907495
1201	11	0.9101872990990856
1201	12	0.9614615820157297
1201	13	1.027413740200764
1201	14	1.8126271718104334
1201	15	1.275644742598665
1201	16	1.665885576413892
1201	17	2.1246469767226444
1201	18	2.6382228520977264
1201	19	1.860408311553094
1201	20	1.6203080102912892
1201	21	1.4482424781997025
1201	22	1.7870945608850448
1201	23	3.384713661490487
1201	24	2.220478947957041
1201	25	2.395157077890012
1202	1	0.9207858707298016
1202	2	1.255401129168444
1202	3	1.0226878486402882
1202	4	1.6177915630609618
1202	5	1.3701878186429752
1202	6	0.8893053745711583
1202	7	0.7241808303609716
1202	8	0.7603409161746129
1202	9	0.9730959389250557
1202	10	1.3754806732250984
1202	11	1.0679841987021064
1202	12	1.1662820561680007
1202	13	1.0463230569158526
1202	14	2.0193034486727797
1202	15	1.4399648951790027
1202	16	1.6357709193869923
1202	17	2.088859347262222
1202	18	2.703759211903453
1202	19	2.05637823903308
1202	20	1.674658545874891
1202	21	1.636530606651057
1202	22	2.0413838869146304
1202	23	3.679721978005979
1202	24	2.3872146601834316
1202	25	2.4642879465777767
1203	1	0.7520044546609093
1203	2	1.0513435673886242
1203	3	0.7515948412916025
1203	4	1.4143655673053104
1203	5	1.2530120640434674
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2211	19	-1.6662650844339737
2211	20	-0.866220182844387
2211	21	-0.588989472279188
2211	22	-0.21994071371093327
2211	23	-5.075160571272942
2211	24	-2.14037020032416
2211	25	-1.3197383355138754
2212	1	0.4827661103766374
2212	2	0.6478039040265458
2212	3	0.47612488879164516
2212	4	1.0324268373935226
2212	5	0.8459246275561512
2212	6	0.38736717822892075
2212	7	0.20001433887473752
2212	8	0.17017484512143
2212	9	-0.032735467423478326
2212	10	-3.708256976554175
2212	11	-0.9925287296140581
2212	12	-0.17867905377524096
2212	13	0.04442690387822523
2212	14	0.8400896584688695
2212	15	0.45203132360027354
2212	16	0.7147786973983443
2212	17	1.1625696688935037
2212	18	1.74270812928836
2212	19	1.0245892964221852
2212	20	0.6633362839450996
2212	21	0.4961942602142315
2212	22	0.739285926014901
2212	23	2.0638422218571435
2212	24	1.0739163629372683
2212	25	1.0297325545239602
2213	1	-1.37742316884205
2213	2	-3.803412600993969
2213	3	-1.6709550136236935
2213	4	-3.173708101655862
2213	5	-1.1089113450469128
2213	6	-0.9328384977597608
2213	7	-0.7025308218892263
2213	8	-0.7189455000066971
2213	9	-0.6109385087855301
2213	10	-0.6376371229311673
2213	11	-0.775257040706947
2213	12	-0.49141313557602473
2213	13	-0.9531502743650151
2213	14	-6.274866504050188
2213	15	-2.6293795945790492
2213	16	-1.846616381182784
2213	17	-0.623500160651723
2213	18	0.013034114586979229
2213	19	-0.6392219178541438
2213	20	-0.7447278226498213
2213	21	-0.9897560161827812
2213	22	-0.7734262668938925
2213	23	-5.623261240965565
2213	24	-3.043705787029502
2213	25	-2.2571813106961827
2214	1	-0.2325165958496207
2214	2	-0.028056720318858197
2214	3	-0.2070843779073286
2214	4	0.06132461920090293
2214	5	-0.14757039362532254
2214	6	-0.31361592348904566
2214	7	-0.4534314412443763
2214	8	-0.46817565745004686
2214	9	-0.4400841097770467
2214	10	-0.04487481901404067
2214	11	-0.5227961945591133
2214	12	-0.38871438228347444
2214	13	-0.5217121278705363
2214	14	0.10085578687080243
2214	15	-0.20075056194161078
2214	16	0.1158726305868143
2214	17	0.40983686809313724
2214	18	0.8354354588595712
2214	19	-0.07476228476303959
2214	20	-0.1093027243555369
2214	21	-0.28031634615427947
2214	22	0.30999968693782165
2214	23	1.6690938202376628
2214	24	0.571552791944157
2214	25	0.4841761305193302
2215	1	-2.443091269290541
2215	2	-4.518187834175354
2215	3	-2.446577379219871
2215	4	-3.9668047926235843
2215	5	-5.1024431552245595
2215	6	-2.3954625445190914
2215	7	-1.6336782448527813
2215	8	-1.9906778118925637
2215	9	-1.652683271674249
2215	10	-3.1028057829936735
2215	11	-1.9421086979779716
2215	12	-1.5985834627228712
2215	13	-1.6756382649423998
2215	14	-0.8971170344221164
2215	15	-1.4608923590829477
2215	16	-2.113131082856036
2215	17	-6.89000032689594
2215	18	-2.948387943512575
2215	19	-2.066707972784215
2215	20	-1.806496090316628
2215	21	-1.7866493459961532
2215	22	-1.5607014043773617
2215	23	-6.412605584290269
2215	24	-3.4894320550687503
2215	25	-3.1128556111520247
2216	1	-1.0323869855276335
2216	2	-0.5592419852259134
2216	3	-0.8232266864284732
2216	4	-3.360182036952441
2216	5	-1.32991704424796
2216	6	-1.0115229752621673
2216	7	-1.0657620478406145
2216	8	-1.0115322097781103
2216	9	-1.1106191556358098
2216	10	-0.7770747315691153
2216	11	-1.2990776302005145
2216	12	-1.9965363434165724
2216	13	-1.3453302924986872
2216	14	-7.242717758423058
2216	15	-3.532623677111065
2216	16	-1.9182719973424938
2216	17	-1.0520865964200397
2216	18	-0.24845530473892552
2216	19	-0.7566016427030249
2216	20	-1.2070355091027452
2216	21	-1.4490691722322993
2216	22	-0.944423942624546
2216	23	0.28005047022502083
2216	24	-0.5759737747463021
2216	25	-0.48688732186117534
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90003.0
3	1.0
4	767.0
5	4632.0
6	5608.0
7	5092.0
8	4711.0
9	4567.0
10	4394.0
11	4228.0
12	4277.0
13	4257.0
14	4309.0
15	4371.0
16	4606.0
17	5011.0
18	5529.0
19	6516.0
20	8103.0
21	10369.0
22	12599.0
23	15657.0
24	18794.0
25	21993.0
26	25355.0
27	29006.0
28	33103.0
29	39533.0
30	46938.0
31	53336.0
32	69719.0
33	92269.0
34	120314.0
35	157574.0
36	223311.0
37	313508.0
38	784195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61699259647525	17.811349208975823	12.017780266390933	25.55387792815799
2	14.06426677083119	19.578223059272773	41.553019773084614	24.80449039681142
3	19.43625051222938	23.98440052069764	28.66008838274941	27.919260584323567
4	10.788800228074322	16.015965202579064	39.1189467470587	34.076287822287924
5	12.548502115257515	37.73313748525889	36.30900857945052	13.409351820033077
6	33.05483805676157	35.89430136915302	17.987720829150096	13.063139744935324
7	29.02335519084665	30.850816663181618	21.508740324520627	18.617087821451104
8	25.7671600923492	36.40827389481897	20.188764478712724	17.635801534119114
9	26.637225795958052	15.185224573400799	19.453540143310878	38.72400948733027
10	13.791565579671447	29.327733683564674	35.100964849208516	21.77973588755536
11	35.015032753037936	22.527593557608576	23.174477785527362	19.282895903826123
12	23.14209808606882	24.8836968235784	31.466331503818388	20.50787358653439
13	29.14846190929574	21.21890968489369	25.219987338022527	24.412641067788048
14	20.71506635580581	21.477682269582136	28.316159432658083	29.49109194195397
15	24.422303333811424	29.30249246113502	22.84330822686879	23.431895978184766
16	22.351713232642815	27.816533732723464	27.695224341946822	22.1365286926869
17	22.388236434861913	27.800931294155706	27.948074162150277	21.862758108832104
18	21.852930794904672	27.05656061086711	29.54064330490186	21.549865289326355
19	23.416419659905987	27.13856911698267	27.584633609989844	21.8603776131215
20	23.47349118517565	27.08556742304008	27.750834655914442	21.69010673586983
21	23.15163804586448	26.631443813755588	27.4113571358673	22.805561004512633
22	22.83844028071229	27.34093744749157	26.906748606627414	22.913873665168722
23	21.277867816299967	26.814589011010614	29.02118072451742	22.886362448171997
24	22.669557138432953	27.618127029709562	27.418323392309148	22.293992439548337
25	22.820414399092716	26.554108310011525	27.299363552559086	23.326113738336677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	170.0
2	292.5
3	415.0
4	415.0
5	415.0
6	811.0
7	1207.0
8	1207.0
9	1207.0
10	1966.0
11	2725.0
12	2725.0
13	2725.0
14	5329.0
15	7933.0
16	7933.0
17	7933.0
18	14807.5
19	21682.0
20	21682.0
21	21682.0
22	35019.0
23	48356.0
24	48356.0
25	48356.0
26	69739.0
27	91122.0
28	91122.0
29	91122.0
30	117458.0
31	143794.0
32	143794.0
33	143794.0
34	170362.0
35	196930.0
36	196930.0
37	196930.0
38	219769.0
39	242608.0
40	242608.0
41	242608.0
42	261908.0
43	281208.0
44	281208.0
45	281208.0
46	305301.0
47	329394.0
48	329394.0
49	329394.0
50	314084.0
51	298774.0
52	298774.0
53	298774.0
54	262061.5
55	225349.0
56	225349.0
57	225349.0
58	191641.0
59	157933.0
60	157933.0
61	157933.0
62	127741.5
63	97550.0
64	97550.0
65	97550.0
66	74975.0
67	52400.0
68	52400.0
69	52400.0
70	38223.0
71	24046.0
72	24046.0
73	24046.0
74	16827.0
75	9608.0
76	9608.0
77	9608.0
78	6584.0
79	3560.0
80	3560.0
81	3560.0
82	2413.5
83	1267.0
84	1267.0
85	1267.0
86	827.0
87	387.0
88	387.0
89	387.0
90	242.0
91	97.0
92	97.0
93	97.0
94	61.0
95	25.0
96	25.0
97	25.0
98	20.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11583365161901314
2	0.04775401989229659
3	0.03520127939675371
4	0.03073411196061745
5	0.034218502560803735
6	0.020370283508781336
7	0.01889611825485637
8	0.02358664406279944
9	0.027830453127128885
10	0.03555865279164461
11	0.019744880067722258
12	0.019610865044638173
13	0.015992459421367802
14	0.020951015275479046
15	0.021710433739622212
16	0.021263716996008587
17	0.026534974570649367
18	0.018985461603579095
19	0.03403981586335828
20	0.022648538901210827
21	0.02742840805787662
22	0.03658610130195595
23	0.02787512480149025
24	0.03832829660204909
25	0.02966199177594475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2238555.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.14406268065149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.06248268805928	63.919405308833575
2	10.372055784348131	15.587968199725397
3	2.5896788669060533	5.8379697329264095
4	0.8759065940289085	2.6327672001641704
5	0.39495915623592204	1.4839417796244674
6	0.195258367181996	0.8803504189467342
7	0.10383393649076834	0.5461752682428758
8	0.07783731055931989	0.4679209394850296
9	0.05884213666032673	0.3979473484920348
>10	0.24534773376821725	3.127782744902511
>50	0.012070707292310233	0.6183744714083883
>100	0.009023350756363093	1.33001973369288
>500	0.0015018709513457506	0.8314377690406305
>1k	0.0010813470849683024	1.502257904122187
>5k	6.007483805379458E-5	0.3763093301509271
>10k+	6.007483805379458E-5	0.4593718502418227
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	10176	0.4545789583012255	No Hit
CCCATGTACTCTGCGTTGATACCAC	8336	0.37238307747631844	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4036	0.18029487772245933	No Hit
GCGTTGATACCACTGCTTCCCATGT	2785	0.12441061309639476	No Hit
GTATCAACGCAGAGTACATGGGAAG	2695	0.12039016240387214	No Hit
CATGTACTCTGCGTTGATACCACTG	2646	0.11820125036016538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.934334872272515E-5
2	0.0	0.0	0.0	0.0	8.934334872272515E-5
3	0.0	0.0	0.0	4.4671674361362575E-5	8.934334872272515E-5
4	0.0	4.4671674361362575E-5	0.0	4.4671674361362575E-5	8.934334872272515E-5
5	4.4671674361362575E-5	4.4671674361362575E-5	0.0	4.4671674361362575E-5	8.934334872272515E-5
6	4.4671674361362575E-5	4.4671674361362575E-5	0.0	4.4671674361362575E-5	8.934334872272515E-5
7	4.4671674361362575E-5	4.4671674361362575E-5	0.0	4.4671674361362575E-5	8.934334872272515E-5
8	8.934334872272515E-5	4.4671674361362575E-5	0.0	4.4671674361362575E-5	8.934334872272515E-5
9	8.934334872272515E-5	4.4671674361362575E-5	0.0	8.934334872272515E-5	1.3401502308408773E-4
10	8.934334872272515E-5	4.4671674361362575E-5	0.0	1.786866974454503E-4	1.3401502308408773E-4
11	8.934334872272515E-5	4.4671674361362575E-5	0.0	1.786866974454503E-4	1.3401502308408773E-4
12	8.934334872272515E-5	4.4671674361362575E-5	0.0	1.786866974454503E-4	1.3401502308408773E-4
13	8.934334872272515E-5	4.4671674361362575E-5	0.0	2.2335837180681288E-4	1.3401502308408773E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	630	0.0	13.272386	4
TAGGCGG	55	0.003065793	12.092687	5
CCAACGA	65	8.0309936E-4	11.691413	19
GTAGGAC	1220	0.0	11.292139	3
TGTAGGA	1360	0.0	10.968525	2
GTCCTAA	555	0.0	10.625097	1
GCGTTTA	185	6.002665E-11	10.268567	13
CTGTAGG	1390	0.0	10.263859	1
GTATAGA	270	0.0	10.215707	1
GACGTGA	270	0.0	10.202467	7
TTATACT	345	0.0	10.190359	4
ATAAGAC	245	0.0	10.082664	3
GTATTAT	285	0.0	10.011764	1
AGGACCG	115	1.0249927E-5	9.914501	5
TCTACAC	480	0.0	9.896846	3
GTCCTAC	1230	0.0	9.820482	1
TAAGACA	455	0.0	9.815068	4
GTCTAAA	225	1.8189894E-12	9.722536	1
CTTATAC	215	1.0913936E-11	9.721925	3
TAGGACG	685	0.0	9.709891	4
>>END_MODULE