FastQCFastQC Report
Thu 26 May 2016
SRR1513100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238555
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC117360.5242667703049512No Hit
CCCATGTACTCTGCGTTGATACCAC109760.4903162977903156No Hit
GAGTACATGGGAAGCAGTGGTATCA45110.2015139230441066No Hit
CATGTACTCTGCGTTGATACCACTG32350.14451286655900794No Hit
GTATCAACGCAGAGTACTTTTTTTT30550.13647196517396265No Hit
GCGTTGATACCACTGCTTCCCATGT29600.1322281561096332No Hit
GTATCAACGCAGAGTACATGGGAAG29540.13196012606346505No Hit
ACGCAGAGTACATGGGAAGCAGTGG27180.12141761091418349No Hit
TATCAACGCAGAGTACATGGGAAGC25880.11561029324720636No Hit
GCTTCCCATGTACTCTGCGTTGATA23170.10350426949527708No Hit
ACTCTGCGTTGATACCACTGCTTCC22770.10171740252082259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG1051.3296813E-913.5996155
ACCGTGC1403.6379788E-1212.8751778
GGACCGT951.0146869E-612.0249246
TAGGACC5800.011.6534644
GTCCTAC12100.011.4961541
TGGCGAG8650.011.19095218
CTACACT4950.011.1544314
GGCGAGG3500.010.84201319
TATGGCG9000.010.75911516
GACCGTG1158.975858E-710.7246457
GGACCGG803.7004295E-410.7096986
GTAGGAC11400.010.688823
TTAGACT2600.010.6181624
TGTAGGA12300.010.6094632
TGGACCG909.320288E-510.5774785
TCCTACA14200.010.5314762
TCCAACG1002.4266825E-510.43940418
AGGACCT8700.010.3951085
AGGACGT7950.010.2980745
ATGGCGA9650.010.23047417
Produced by FastQC (version 0.11.5)