FastQCFastQC Report
Thu 26 May 2016
SRR1513099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846241
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC274641.4875631079582785No Hit
CCCATGTACTCTGCGTTGATACCAC223711.211705297412418No Hit
GAGTACATGGGAAGCAGTGGTATCA124620.6749931346990994No Hit
GCGTTGATACCACTGCTTCCCATGT76610.41495124417668117No Hit
GTATCAACGCAGAGTACATGGGAAG74490.40346845292678474No Hit
CATGTACTCTGCGTTGATACCACTG74310.40249349895273695No Hit
TATCAACGCAGAGTACATGGGAAGC58660.3177266673202469No Hit
ACGCAGAGTACATGGGAAGCAGTGG57970.31398934375306364No Hit
GCTTCCCATGTACTCTGCGTTGATA51060.2765619439715617No Hit
ACTCTGCGTTGATACCACTGCTTCC49870.27011641492091226No Hit
GGTATCAACGCAGAGTACATGGGAA36850.19959474413145414No Hit
CAGTGGTATCAACGCAGAGTACATG29800.16140904681458162No Hit
CCACTGCTTCCCATGTACTCTGCGT27720.1501429120033625No Hit
GCAGTGGTATCAACGCAGAGTACAT24210.13113130950943025No Hit
GTGGTATCAACGCAGAGTACATGGG23900.12945222210968124No Hit
GTATCAACGCAGAGTACTTTTTTTT23420.12685234484555377No Hit
ATACCACTGCTTCCCATGTACTCTG23010.1246316163491115No Hit
CTGCTTCCCATGTACTCTGCGTTGA22370.12116511333027487No Hit
GGGAAGCAGTGGTATCAACGCAGAG21620.11710280510507567No Hit
GTACTCTGCGTTGATACCACTGCTT21620.11710280510507567No Hit
ACATGGGAAGCAGTGGTATCAACGC21400.11591119469235057No Hit
CCCATGTACTCTGCGTTTATACCAC20700.11211970701549796No Hit
AAGCAGTGGTATCAACGCAGAGTAC20530.11119891715111949No Hit
CATGGGAAGCAGTGGTATCAACGCA19650.10643247550021909No Hit
GTACATGGGAAGCAGTGTTATCAAC19510.10567417796484857No Hit
TACCACTGCTTCCCATGTACTCTGC19240.10421174700377686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC1250.014.43907419
TCGTACA655.4471486E-513.1554982
TCGAACT1251.03682396E-1012.91917119
GCGTTTA4500.012.45340613
CTCGAAC1405.2750693E-1112.21350118
TGGACCG550.003066560312.0920985
TACACCG550.003066560312.0920985
TATCGAC802.8689252E-511.87423819
TGCGTTT4900.011.8244912
CGTTTAT4600.011.76970914
CGTAGAC658.021694E-411.6928253
GCACCGT658.030816E-411.6912416
GTCCTAC5000.011.4141131
GGCGAGG1900.010.99929419
GATACGA1303.2698154E-810.96083518
TAGGACC2900.010.8117024
TTACACC1605.4387783E-1010.6888424
AGTGTTA4350.010.48094914
TAAGCTG1002.396438E-510.4510285
ACACCGT1002.4014593E-510.4490466
Produced by FastQC (version 0.11.5)