##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513099_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846241 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.19517766098792 31.0 30.0 34.0 24.0 34.0 2 28.928538040266684 31.0 30.0 34.0 16.0 34.0 3 29.288703912436134 31.0 30.0 34.0 23.0 34.0 4 32.100751202037 35.0 32.0 37.0 19.0 37.0 5 32.33776522133351 35.0 33.0 37.0 20.0 37.0 6 32.81350701235646 37.0 33.0 37.0 26.0 37.0 7 32.8096353617973 37.0 35.0 37.0 26.0 37.0 8 32.79024298561239 37.0 35.0 37.0 26.0 37.0 9 34.142603267937396 39.0 35.0 39.0 25.0 39.0 10 33.649710411587655 39.0 34.0 39.0 18.0 39.0 11 34.01712939968292 39.0 34.0 39.0 25.0 39.0 12 33.84942756660696 39.0 34.0 39.0 22.0 39.0 13 33.92627289720031 39.0 35.0 39.0 23.0 39.0 14 34.2335420998667 40.0 33.0 41.0 16.0 41.0 15 34.76186207542786 40.0 34.0 41.0 20.0 41.0 16 34.325855075258325 39.0 34.0 41.0 17.0 41.0 17 33.87206274803777 39.0 33.0 41.0 11.0 41.0 18 33.28116101852358 39.0 32.0 41.0 10.0 41.0 19 34.15725195139746 39.0 33.0 41.0 18.0 41.0 20 34.435908421489934 39.0 34.0 41.0 18.0 41.0 21 34.45283795560818 39.0 34.0 41.0 17.0 41.0 22 34.05936711404416 39.0 34.0 41.0 10.0 41.0 23 32.40607591316627 39.0 31.0 41.0 2.0 41.0 24 33.49138384425435 39.0 33.0 41.0 2.0 41.0 25 33.232788677101205 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6350136605698147 1101 2 1.0634983136649332 1101 3 0.7987864736238066 1101 4 0.8023111392785367 1101 5 1.0286756508895039 1101 6 0.6591581219590239 1101 7 0.8085911343779628 1101 8 0.6761759546351698 1101 9 0.7800781759250128 1101 10 1.1072183987484152 1101 11 0.7922527668463886 1101 12 0.8162474063851093 1101 13 0.8126519517219251 1101 14 -0.039680851075189594 1101 15 0.40485298972178896 1101 16 1.063685462266804 1101 17 1.6673635558960882 1101 18 -4.5166394648477315 1101 19 -0.8922683959903353 1101 20 0.19236230824199652 1101 21 0.530221585313079 1101 22 1.200662899683664 1101 23 3.0388524708776785 1101 24 1.898155699604196 1101 25 2.081040111909182 1102 1 1.033391352487559 1102 2 1.3352244767808976 1102 3 1.0760036367581591 1102 4 1.5561542220447464 1102 5 1.4690631504626879 1102 6 1.1352965480588395 1102 7 0.9137907151755513 1102 8 0.9166517073477678 1102 9 1.0669011417032834 1102 10 1.5331836017963028 1102 11 1.0412182877106417 1102 12 1.1887461784582385 1102 13 1.178737531001353 1102 14 2.083636757790046 1102 15 1.5444070749753749 1102 16 1.6243341491073693 1102 17 1.4424953446243762 1102 18 1.7576276532407462 1102 19 1.489077096664431 1102 20 1.474156815009387 1102 21 1.5828825750615252 1102 22 1.8459538329868792 1102 23 3.486042291298304 1102 24 2.330764971962317 1102 25 2.514166820828791 1103 1 0.8781556573865288 1103 2 1.1052781143521209 1103 3 0.8692732218955399 1103 4 1.284459110408072 1103 5 1.2039176290540539 1103 6 0.6857035360560104 1103 7 0.6908400389391431 1103 8 0.6360751100855921 1103 9 0.7101895516926504 1103 10 1.0923605845702369 1103 11 0.8360027431553974 1103 12 0.9104831820190569 1103 13 0.7876064225357382 1103 14 1.4580525908162656 1103 15 1.0000529397387012 1103 16 1.2621785974994353 1103 17 1.7059544509476723 1103 18 1.7844846188336945 1103 19 1.2090481435118008 1103 20 1.1191372079980582 1103 21 1.08447539616445 1103 22 1.4386086939083427 1103 23 3.0069600160895718 1103 24 1.9983734998854032 1103 25 2.0894593351661683 1104 1 0.5352610243985403 1104 2 1.0110565648832726 1104 3 0.692196202924908 1104 4 1.2157683177984495 1104 5 1.0158026012049959 1104 6 0.5933985031366475 1104 7 0.49206841080720665 1104 8 0.5237307475680808 1104 9 0.5957550337345836 1104 10 0.997015183982846 1104 11 0.6280887289266133 1104 12 0.6843510999239371 1104 13 0.5577670592739636 1104 14 1.3724003412387802 1104 15 0.9195737794553338 1104 16 1.3149148570244336 1104 17 1.747133601770436 1104 18 2.318605374689753 1104 19 1.4195945129471 1104 20 0.8749656283759961 1104 21 0.9637113642797814 1104 22 1.405306104262074 1104 23 2.701809001515457 1104 24 1.7236704152514974 1104 25 1.7691215361468267 1105 1 0.7621944174546975 1105 2 1.179570525473828 1105 3 0.9394738249205794 1105 4 1.055408731561812 1105 5 1.170447002837932 1105 6 0.7527590271130009 1105 7 0.5208663899819186 1105 8 0.7230968019996382 1105 9 0.870657127156619 1105 10 1.2557426003385217 1105 11 0.9312483252186183 1105 12 0.9158170285871563 1105 13 0.9281494910135919 1105 14 1.640570286185607 1105 15 1.0350879251561693 1105 16 1.4587047880918007 1105 17 2.006554610339599 1105 18 1.9193015569235854 1105 19 1.4607282257945897 1105 20 1.1229101806109938 1105 21 1.0344295845396871 1105 22 1.562476537503919 1105 23 3.1133289549560317 1105 24 1.9640147813097428 1105 25 1.9910062939532622 1106 1 -0.3053251276767881 1106 2 0.11986903293651352 1106 3 -0.022502010189583643 1106 4 -2.4787064070309377 1106 5 -0.2648763802466476 1106 6 -0.2255636452395251 1106 7 -0.23535251549071745 1106 8 -0.053022600985435986 1106 9 0.051893800930606915 1106 10 0.032217369350604486 1106 11 0.09933645599331697 1106 12 0.25767914656867674 1106 13 0.06709760757365046 1106 14 0.862674053065497 1106 15 0.17474677377024506 1106 16 -0.8781822055116137 1106 17 -5.507660030740716 1106 18 -6.6972584999349785 1106 19 -2.54424689858352 1106 20 -1.0991935862688962 1106 21 -0.5899485249130549 1106 22 -0.8561446423112926 1106 23 -4.544098195008434 1106 24 -1.6774208974393723 1106 25 -0.2943526257340139 1107 1 0.43956459052275676 1107 2 0.7381153219315557 1107 3 0.4424909231665275 1107 4 0.7619318614157393 1107 5 0.7786705646779026 1107 6 0.337507723056504 1107 7 0.3003271950690092 1107 8 0.2549447719018758 1107 9 0.2357193587369366 1107 10 0.7071539692409985 1107 11 0.30221643694185474 1107 12 0.4157186650458655 1107 13 0.29402599521770867 1107 14 1.1420601610272456 1107 15 0.5882238362059482 1107 16 -5.3354842932592845 1107 17 -1.0145521348108204 1107 18 0.6221123875630141 1107 19 0.45469735739243333 1107 20 0.3953990074741469 1107 21 0.4124820899626229 1107 22 0.5592272125514555 1107 23 2.3087795302348297 1107 24 1.4772058499001872 1107 25 1.1734048408353246 1108 1 0.4641792667274949 1108 2 0.6904776622637456 1108 3 0.5386209464557616 1108 4 0.9722634821988763 1108 5 0.8274407795987244 1108 6 0.4289217674184371 1108 7 0.5009572250054077 1108 8 0.4914692255959423 1108 9 0.6187234159218491 1108 10 1.069470129937912 1108 11 0.5323040223427569 1108 12 0.6327948572140585 1108 13 0.40562275188693064 1108 14 1.1748102362610169 1108 15 0.9276581749524837 1108 16 1.1364796767148846 1108 17 1.1829236362899849 1108 18 1.1020082326044118 1108 19 0.956604246388487 1108 20 0.794125532423358 1108 21 0.8877084861986404 1108 22 1.1612110877492086 1108 23 2.8082948918222215 1108 24 1.6538530464301289 1108 25 1.8562584304831589 1109 1 0.3138884267470772 1109 2 0.5869477479799485 1109 3 0.34745605074541075 1109 4 0.4772135210735229 1109 5 0.29272595635840304 1109 6 0.15747687823519385 1109 7 0.11268225164174339 1109 8 0.17907253138106682 1109 9 0.22386158890925145 1109 10 0.6879286300482974 1109 11 0.3232119134169409 1109 12 0.3494392069515371 1109 13 0.13792998641145715 1109 14 -0.650873909600513 1109 15 0.04436635590678861 1109 16 -0.30513539705096093 1109 17 0.7065258286283154 1109 18 1.4399942857235075 1109 19 0.5057694012631728 1109 20 0.5006583935016735 1109 21 0.2891271709876122 1109 22 0.20039673580403417 1109 23 -4.509237815677228 1109 24 -1.55824992820056 1109 25 -0.31948522718954564 1110 1 0.5578226229111181 1110 2 0.8630405802477377 1110 3 0.5747885524346863 1110 4 1.1277358974964145 1110 5 0.8106257952273666 1110 6 0.4689639617634427 1110 7 0.4435687322280515 1110 8 0.42508843657364537 1110 9 0.48036660476246595 1110 10 0.7980863825302364 1110 11 0.4704116119459698 1110 12 0.6102752691169258 1110 13 0.3176690580887822 1110 14 1.1960048263005234 1110 15 0.6236467239092818 1110 16 1.1316440613408432 1110 17 1.373119726440052 1110 18 1.9492892074969888 1110 19 1.2465134628048347 1110 20 0.8448238906688843 1110 21 0.6868245565066076 1110 22 0.8795016788883956 1110 23 2.365159242381921 1110 24 1.3568132247699651 1110 25 1.6083098765060697 1111 1 0.6147268869530471 1111 2 0.9407732052075843 1111 3 0.6564611859998557 1111 4 1.1901868836873035 1111 5 0.8508922123164737 1111 6 0.4285808741225168 1111 7 0.4712427319752237 1111 8 0.2573921464884421 1111 9 0.4621815392889417 1111 10 0.7595658486290873 1111 11 0.5378134422587451 1111 12 0.5742288235676298 1111 13 0.4627418148190827 1111 14 -0.28071649924395103 1111 15 0.2623736927828304 1111 16 0.7780736061285367 1111 17 1.3195181900071802 1111 18 1.911508012331275 1111 19 1.038915959972151 1111 20 0.9872000035911341 1111 21 0.7374812632657282 1111 22 0.9709391505470748 1111 23 0.7751632203142549 1111 24 0.8139638190823391 1111 25 0.41092928116152905 1112 1 0.6305644317898 1112 2 0.88698743607819 1112 3 0.7292746994823958 1112 4 1.2554737816142136 1112 5 1.0318381102548386 1112 6 0.357455399970263 1112 7 0.4347952926839014 1112 8 0.43384210695187875 1112 9 0.4242239179002354 1112 10 0.8037700177309759 1112 11 0.4963092192954477 1112 12 0.4873593171443744 1112 13 0.3591383722731578 1112 14 0.869895572586536 1112 15 0.5798580214061531 1112 16 0.9893409535116717 1112 17 1.2354187641468286 1112 18 1.2877402352028895 1112 19 0.8039626589556619 1112 20 0.6925274694693613 1112 21 0.7022008974665752 1112 22 0.7325132787372155 1112 23 2.180805374312037 1112 24 1.2572452391898281 1112 25 1.3354930610861189 1113 1 0.812318178813193 1113 2 1.2004739187788473 1113 3 0.845445031461665 1113 4 0.9806845954536882 1113 5 1.302464248369425 1113 6 0.6661821123949707 1113 7 0.5800789458566129 1113 8 0.45593203551329964 1113 9 0.7233106247219183 1113 10 1.0083280790841087 1113 11 0.79592541203953 1113 12 0.7989295650782537 1113 13 0.6633294354472739 1113 14 1.3151316000413615 1113 15 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1.3532418836073674 1203 14 2.241545445559936 1203 15 1.8334018793327402 1203 16 2.16234135852401 1203 17 2.479081548422535 1203 18 2.9586419399366264 1203 19 2.182380901693051 1203 20 1.9394239704082565 1203 21 1.7664861734272534 1203 22 2.1279884318441518 1203 23 3.804593589680408 1203 24 2.5824883079560124 1203 25 2.8127566504772545 1204 1 0.719806843495725 1204 2 1.1752331383255594 1204 3 0.8444521786857919 1204 4 1.4736042194408014 1204 5 1.075458851485898 1204 6 0.777954518461577 1204 7 0.6640523431622114 1204 8 0.6608646442966588 1204 9 0.7912455641893104 1204 10 1.1333772531033404 1204 11 0.8338118447274496 1204 12 0.8242200005840985 1204 13 0.7181916892280356 1204 14 1.6262566283602595 1204 15 0.9312967581437448 1204 16 1.344230621720719 1204 17 1.8232017538933292 1204 18 2.3101229181716363 1204 19 1.442825544575932 1204 20 1.2433128077841218 1204 21 1.1561473195596648 1204 22 1.4496232031884233 1204 23 2.8140800676933964 1204 24 1.7695897977035528 1204 25 1.9702434700170457 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0.8610622444274085 2208 10 1.176140610288769 2208 11 0.8299690075213917 2208 12 0.8411901629155167 2208 13 0.7426484249155152 2208 14 1.5666541525679847 2208 15 1.1903828789585873 2208 16 1.4491099053625902 2208 17 1.8800443047248123 2208 18 2.399756425438156 2208 19 1.5236280125749744 2208 20 1.1946941180665718 2208 21 1.0290990941345655 2208 22 1.5141467578308507 2208 23 2.9104149535345627 2208 24 1.7561256555101679 2208 25 1.9579572534415348 2209 1 -0.19141494815471205 2209 2 0.30054433507384104 2209 3 0.1478826640091171 2209 4 -2.4395747397266945 2209 5 -0.36325790263572344 2209 6 -0.21059102544256092 2209 7 -0.15077260690539163 2209 8 -0.17700825832113765 2209 9 0.13462626340125894 2209 10 0.10089750588837632 2209 11 0.1472669251628389 2209 12 0.120583048380972 2209 13 0.10548552096290109 2209 14 0.7911606408556366 2209 15 0.36836918555488296 2209 16 0.8262414029852678 2209 17 1.0254273659326074 2209 18 1.2823804745990728 2209 19 0.4648470679160539 2209 20 0.4748056100298115 2209 21 0.18117619459164302 2209 22 -0.4687057518051816 2209 23 1.6982822645847477 2209 24 0.8742224989758753 2209 25 1.0575565204578297 2210 1 0.5599293771461049 2210 2 0.5526163480369206 2210 3 0.4447896660906423 2210 4 1.1483639876707272 2210 5 0.9366079779105476 2210 6 0.7442135179214588 2210 7 0.4728127483896145 2210 8 0.44842559797657344 2210 9 0.4152979852892784 2210 10 0.8887271353852313 2210 11 0.6253451962152567 2210 12 0.5882937039530276 2210 13 0.5310337544879005 2210 14 0.5663448095544368 2210 15 0.3755307764149194 2210 16 0.8066813747077219 2210 17 1.3075641623733105 2210 18 2.076081943160432 2210 19 1.17120404027375 2210 20 0.9060703721936889 2210 21 0.8035400610133792 2210 22 0.051935924575040815 2210 23 2.164603475028777 2210 24 1.3137048401190938 2210 25 1.5332570073099703 2211 1 -0.6417195407515237 2211 2 -0.13340220228942812 2211 3 -0.27283314394027514 2211 4 0.48123928825472007 2211 5 -2.755594014531912 2211 6 -0.9452402780249507 2211 7 -0.574250116605235 2211 8 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0.6603809076203078 2212 20 0.28668058991702594 2212 21 0.3091898215537938 2212 22 0.4392515495999376 2212 23 1.7967676375904134 2212 24 1.0239606182912695 2212 25 0.9971350246905359 2213 1 -1.4703422179373398 2213 2 -4.017973150645457 2213 3 -1.8234622854741502 2213 4 -3.2198763180017593 2213 5 -1.1091938534791694 2213 6 -0.9198800893029748 2213 7 -0.8370604518372176 2213 8 -0.6708817113716847 2213 9 -0.586729219281068 2213 10 -0.6306278109295462 2213 11 -0.6992845785274326 2213 12 -0.5614652143718146 2213 13 -0.9673473494373539 2213 14 -6.271558531880302 2213 15 -2.6474511923495143 2213 16 -1.6817011161906308 2213 17 -0.5484607727110813 2213 18 0.11199167169409918 2213 19 -0.5118754145580411 2213 20 -0.6442536352573427 2213 21 -1.0943659607322545 2213 22 -0.8209345889034694 2213 23 -5.330892139619763 2213 24 -2.851894598537193 2213 25 -1.8542473598996452 2214 1 -0.3044375390418921 2214 2 -0.22456163763091652 2214 3 -0.29759167812895626 2214 4 -0.08950622852149337 2214 5 -0.2981568218922419 2214 6 -0.4947310970171799 2214 7 -0.6105248266921848 2214 8 -0.5846105086233209 2214 9 -0.5652811217979448 2214 10 -0.12552217214457784 2214 11 -0.6933016822192783 2214 12 -0.5471257326895156 2214 13 -0.6236050254406749 2214 14 -0.10662562793151409 2214 15 -0.4637930850750678 2214 16 -0.11297336598829588 2214 17 0.23500207401788487 2214 18 0.844314366629213 2214 19 -0.25619050282146816 2214 20 -0.37431300578262494 2214 21 -0.4165051107546063 2214 22 0.13366874920289717 2214 23 1.345698667853334 2214 24 0.4015315165703157 2214 25 0.20212181270501617 2215 1 -2.6189119515804684 2215 2 -4.29558096566149 2215 3 -2.5245864688872928 2215 4 -4.0241513144051915 2215 5 -5.188688545838179 2215 6 -2.5354557979651133 2215 7 -1.7725755715736682 2215 8 -2.194575769513282 2215 9 -1.7414264996114923 2215 10 -3.2490276700664076 2215 11 -2.1117348584484965 2215 12 -1.8831851888875804 2215 13 -1.7876377519137847 2215 14 -1.0117774585320447 2215 15 -1.6564022229666904 2215 16 -2.3731741747260457 2215 17 -7.189767333900729 2215 18 -3.219787686412115 2215 19 -2.2446578115532567 2215 20 -2.0149619891193353 2215 21 -1.8432816311458282 2215 22 -1.5972936877126358 2215 23 -6.2644208446509 2215 24 -3.6045290336876583 2215 25 -3.4848762060716503 2216 1 -1.1975633612452832 2216 2 -0.6730718236625641 2216 3 -0.937249938811096 2216 4 -3.0902531794011097 2216 5 -1.2904531941636392 2216 6 -1.0989340892801067 2216 7 -1.1679426806762976 2216 8 -0.9974090102621567 2216 9 -1.0259647490806074 2216 10 -0.708227100533918 2216 11 -1.298723579400189 2216 12 -2.0383741440253296 2216 13 -1.4126274488317634 2216 14 -6.752283279161194 2216 15 -3.3257922377874998 2216 16 -1.8235488526212222 2216 17 -0.9916852545736106 2216 18 -0.35863999132748603 2216 19 -0.9177318229549485 2216 20 -1.3427552601621358 2216 21 -1.5869362612076117 2216 22 -1.0043752056976203 2216 23 0.10493373069382983 2216 24 -0.6910822169205844 2216 25 -0.5151732589056479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90808.0 3 4.0 4 764.0 5 4613.0 6 5779.0 7 5318.0 8 4986.0 9 4555.0 10 4235.0 11 4240.0 12 4195.0 13 3970.0 14 4038.0 15 4138.0 16 4174.0 17 4356.0 18 5011.0 19 5851.0 20 7186.0 21 9095.0 22 10930.0 23 13701.0 24 16065.0 25 18876.0 26 21269.0 27 24270.0 28 27085.0 29 32986.0 30 38494.0 31 44215.0 32 57000.0 33 74776.0 34 97304.0 35 128909.0 36 181799.0 37 253992.0 38 627254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.759395895092844 17.20414947974252 11.654320639546075 25.382133985618566 2 13.752054435983151 19.976243594196806 40.046775842813226 26.22492612700682 3 20.50281206315493 24.675068540653005 27.883367106988437 26.938752289203627 4 11.222807977590794 16.109217790829348 37.287868362166584 35.38010586941328 5 12.514439592115387 38.07349291836889 35.54512846631484 13.866939023200874 6 33.86440046242875 33.687360028083766 18.031362377065786 14.416877132421693 7 28.746782729480575 30.989540586500762 21.122792875225432 19.140883808793234 8 27.248466277610795 34.81620252286769 20.246760817320716 17.6885703822008 9 27.892589072965013 14.936063376412854 18.97787005061401 38.19347750000813 10 15.091714338038884 29.23442611759071 33.58130754834956 22.092551996020855 11 34.259340521225084 23.407455081094025 21.524940380804406 20.808264016876485 12 24.16229437592942 25.67564419620728 29.872013998553555 20.290047429309745 13 29.38038331942925 20.749195549247553 24.185256611664265 25.685164519658937 14 21.627984963245115 21.994694121005896 27.212918303873984 29.164402611875005 15 27.13893487739128 26.74421628368598 22.200835160834554 23.916013678088188 16 22.067250964589984 27.240660461074555 28.374385517443883 22.317703056891578 17 23.918965336026325 27.301874873359633 27.342237257134933 21.43692253347911 18 23.456159435207237 25.7523094225306 29.07716277447078 21.714368367791383 19 23.961763126654905 27.33579930788724 27.807393914240652 20.89504365121721 20 24.28238478391363 26.470986588190932 26.95038995880457 22.29623866909086 21 22.688842523639686 26.520224236359468 28.23320408011785 22.557729159882996 22 23.17437715478356 26.865149222232514 25.135687360716254 24.82478626226767 23 20.75063416093005 26.935017060852363 28.58757380484639 23.7267749733712 24 22.7728024413345 29.06220808128534 25.729918701653926 22.435070775726235 25 23.00460046497692 25.763904202400955 25.668709090347587 25.562786242274537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 180.0 1 180.0 2 366.0 3 552.0 4 552.0 5 552.0 6 1027.0 7 1502.0 8 1502.0 9 1502.0 10 2231.0 11 2960.0 12 2960.0 13 2960.0 14 4638.5 15 6317.0 16 6317.0 17 6317.0 18 11118.0 19 15919.0 20 15919.0 21 15919.0 22 24640.5 23 33362.0 24 33362.0 25 33362.0 26 47271.5 27 61181.0 28 61181.0 29 61181.0 30 79163.0 31 97145.0 32 97145.0 33 97145.0 34 115419.5 35 133694.0 36 133694.0 37 133694.0 38 152767.0 39 171840.0 40 171840.0 41 171840.0 42 195686.0 43 219532.0 44 219532.0 45 219532.0 46 273515.5 47 327499.0 48 327499.0 49 327499.0 50 302969.0 51 278439.0 52 278439.0 53 278439.0 54 236449.0 55 194459.0 56 194459.0 57 194459.0 58 163813.5 59 133168.0 60 133168.0 61 133168.0 62 109248.0 63 85328.0 64 85328.0 65 85328.0 66 66012.5 67 46697.0 68 46697.0 69 46697.0 70 34305.5 71 21914.0 72 21914.0 73 21914.0 74 15512.5 75 9111.0 76 9111.0 77 9111.0 78 6340.5 79 3570.0 80 3570.0 81 3570.0 82 2443.0 83 1316.0 84 1316.0 85 1316.0 86 858.5 87 401.0 88 401.0 89 401.0 90 264.0 91 127.0 92 127.0 93 127.0 94 73.5 95 20.0 96 20.0 97 20.0 98 14.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1114697376994661 2 0.04571450856090835 3 0.0336359121046494 4 0.030927706621183257 5 0.0336359121046494 6 0.018144976739223103 7 0.018632453726247007 8 0.02193646441607569 9 0.027461203602346603 10 0.03352758388531075 11 0.019444915371286845 12 0.018199140848892424 13 0.014136832623693222 14 0.01971573591963346 15 0.019986556467980074 16 0.0205281975646733 17 0.025077982776896407 18 0.017603335642529874 19 0.03184849648556175 20 0.02193646441607569 21 0.027623695931354574 22 0.03455670196902788 23 0.026811234286314736 24 0.037535728000840624 25 0.030223573195482065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1846241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.26597181374888 #Duplication Level Percentage of deduplicated Percentage of total 1 84.92197459050765 57.97281123763492 2 10.31474671091869 14.082924164670683 3 2.5579316559147336 5.238590709725135 4 0.8823579932339032 2.4094010358296662 5 0.42239543593718 1.441761746197185 6 0.23308439828841482 0.9547039778268925 7 0.15155492655494016 0.7242231041104028 8 0.0972065782061599 0.530872122234615 9 0.06722061845259633 0.4129992760128937 >10 0.3132758895528467 3.678157668086479 >50 0.019660618196369276 0.9226076006629665 >100 0.013720234648339848 1.894794234935063 >500 0.0017046223558828083 0.7731248448168373 >1k 0.0024351747941182976 3.3326601502991853 >5k 4.8703495882365953E-4 2.178292549437348 >10k+ 2.4351747941182977E-4 3.4520755775196754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 27464 1.4875631079582785 No Hit CCCATGTACTCTGCGTTGATACCAC 22371 1.211705297412418 No Hit GAGTACATGGGAAGCAGTGGTATCA 12462 0.6749931346990994 No Hit GCGTTGATACCACTGCTTCCCATGT 7661 0.41495124417668117 No Hit GTATCAACGCAGAGTACATGGGAAG 7449 0.40346845292678474 No Hit CATGTACTCTGCGTTGATACCACTG 7431 0.40249349895273695 No Hit TATCAACGCAGAGTACATGGGAAGC 5866 0.3177266673202469 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5797 0.31398934375306364 No Hit GCTTCCCATGTACTCTGCGTTGATA 5106 0.2765619439715617 No Hit ACTCTGCGTTGATACCACTGCTTCC 4987 0.27011641492091226 No Hit GGTATCAACGCAGAGTACATGGGAA 3685 0.19959474413145414 No Hit CAGTGGTATCAACGCAGAGTACATG 2980 0.16140904681458162 No Hit CCACTGCTTCCCATGTACTCTGCGT 2772 0.1501429120033625 No Hit GCAGTGGTATCAACGCAGAGTACAT 2421 0.13113130950943025 No Hit GTGGTATCAACGCAGAGTACATGGG 2390 0.12945222210968124 No Hit GTATCAACGCAGAGTACTTTTTTTT 2342 0.12685234484555377 No Hit ATACCACTGCTTCCCATGTACTCTG 2301 0.1246316163491115 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2237 0.12116511333027487 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2162 0.11710280510507567 No Hit GTACTCTGCGTTGATACCACTGCTT 2162 0.11710280510507567 No Hit ACATGGGAAGCAGTGGTATCAACGC 2140 0.11591119469235057 No Hit CCCATGTACTCTGCGTTTATACCAC 2070 0.11211970701549796 No Hit AAGCAGTGGTATCAACGCAGAGTAC 2053 0.11119891715111949 No Hit CATGGGAAGCAGTGGTATCAACGCA 1965 0.10643247550021909 No Hit GTACATGGGAAGCAGTGTTATCAAC 1951 0.10567417796484857 No Hit TACCACTGCTTCCCATGTACTCTGC 1924 0.10421174700377686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.4164109669322694E-5 0.0 3 0.0 0.0 0.0 5.4164109669322694E-5 0.0 4 0.0 0.0 0.0 5.4164109669322694E-5 5.4164109669322694E-5 5 0.0 0.0 0.0 5.4164109669322694E-5 5.4164109669322694E-5 6 0.0 0.0 0.0 5.4164109669322694E-5 5.4164109669322694E-5 7 0.0 0.0 0.0 5.4164109669322694E-5 5.4164109669322694E-5 8 0.0 0.0 0.0 5.4164109669322694E-5 5.4164109669322694E-5 9 0.0 0.0 0.0 5.4164109669322694E-5 5.4164109669322694E-5 10 0.0 0.0 0.0 1.624923290079681E-4 5.4164109669322694E-5 11 0.0 0.0 0.0 2.1665643867729078E-4 5.4164109669322694E-5 12 0.0 0.0 0.0 2.7082054834661346E-4 5.4164109669322694E-5 13 0.0 0.0 0.0 3.249846580159362E-4 5.4164109669322694E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGAC 125 0.0 14.439074 19 TCGTACA 65 5.4471486E-5 13.155498 2 TCGAACT 125 1.03682396E-10 12.919171 19 GCGTTTA 450 0.0 12.453406 13 CTCGAAC 140 5.2750693E-11 12.213501 18 TGGACCG 55 0.0030665603 12.092098 5 TACACCG 55 0.0030665603 12.092098 5 TATCGAC 80 2.8689252E-5 11.874238 19 TGCGTTT 490 0.0 11.82449 12 CGTTTAT 460 0.0 11.769709 14 CGTAGAC 65 8.021694E-4 11.692825 3 GCACCGT 65 8.030816E-4 11.691241 6 GTCCTAC 500 0.0 11.414113 1 GGCGAGG 190 0.0 10.999294 19 GATACGA 130 3.2698154E-8 10.960835 18 TAGGACC 290 0.0 10.811702 4 TTACACC 160 5.4387783E-10 10.688842 4 AGTGTTA 435 0.0 10.480949 14 TAAGCTG 100 2.396438E-5 10.451028 5 ACACCGT 100 2.4014593E-5 10.449046 6 >>END_MODULE