FastQCFastQC Report
Thu 26 May 2016
SRR1513099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846241
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC314091.7012405206037564No Hit
CCCATGTACTCTGCGTTGATACCAC295411.600061963741462No Hit
GAGTACATGGGAAGCAGTGGTATCA129300.7003419380243425No Hit
CATGTACTCTGCGTTGATACCACTG88950.48178975550862535No Hit
GTATCAACGCAGAGTACATGGGAAG81240.4400292269535776No Hit
GCGTTGATACCACTGCTTCCCATGT78410.4247007839171592No Hit
ACGCAGAGTACATGGGAAGCAGTGG74780.40503921210719507No Hit
TATCAACGCAGAGTACATGGGAAGC70610.3824527783750875No Hit
GCTTCCCATGTACTCTGCGTTGATA65130.35277084627629873No Hit
ACTCTGCGTTGATACCACTGCTTCC61920.3353841670724461No Hit
GGTATCAACGCAGAGTACATGGGAA47640.25803781846465335No Hit
GTATCAACGCAGAGTACTTTTTTTT38090.20631109373045015No Hit
CCACTGCTTCCCATGTACTCTGCGT35360.19152429179072503No Hit
CAGTGGTATCAACGCAGAGTACATG35110.190170189048992No Hit
ATACCACTGCTTCCCATGTACTCTG29470.159621631195494No Hit
CATGGGAAGCAGTGGTATCAACGCA29230.15832169256343023No Hit
ACATGGGAAGCAGTGGTATCAACGC28550.1546385331059163No Hit
GTGGTATCAACGCAGAGTACATGGG28290.1532302662545139No Hit
GCAGTGGTATCAACGCAGAGTACAT27960.15144285063542626No Hit
TATCAACGCAGAGTACTTTTTTTTT27420.1485179887132828No Hit
CTGCTTCCCATGTACTCTGCGTTGA26550.14380571117205176No Hit
TACCACTGCTTCCCATGTACTCTGC25750.13947258239850593No Hit
GGGAAGCAGTGGTATCAACGCAGAG25450.13784765910842625No Hit
AAGCAGTGGTATCAACGCAGAGTAC23960.12977720676769716No Hit
GTACTCTGCGTTGATACCACTGCTT23070.12495660100712745No Hit
GGTATCAACGCAGAGTACTTTTTTT22490.12181508264630674No Hit
CTTCCCATGTACTCTGCGTTGATAC21440.11612785113102786No Hit
CCATGTACTCTGCGTTGATACCACT21290.115315389485988No Hit
GATACCACTGCTTCCCATGTACTCT20970.1135821379765697No Hit
GCAGAGTACATGGGAAGCAGTGGTA19440.10529502919716333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG903.6858182E-813.70918718
CAACCGT500.001477510713.3263716
GTCCTAA2800.012.9300171
GGACCGA550.003023623712.1148826
TGGACCG752.0364003E-411.4226035
GTCCTAC5100.011.0218731
TTACTCT3100.010.7470734
TAGGACT2250.010.5764854
CTAGACA1752.2737368E-1010.3347364
GTCCTAT1303.6927304E-710.2602581
TTTACTC2700.010.2239353
GCCGGTT750.002678546110.11931911
CGTGCAC750.002681556910.11794910
GGACCGT856.475176E-410.0787686
CGGTTTC856.637152E-410.0517213
TAATACT1909.640644E-1110.0198284
ACCGTGC951.6659542E-49.9869818
ACCGTCC951.6659542E-49.9869818
GTATAAC1053.9775157E-59.9810681
TAGGGCT2105.456968E-129.9721154
Produced by FastQC (version 0.11.5)