FastQCFastQC Report
Thu 26 May 2016
SRR1513098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725573
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC225131.3046680725764717No Hit
CCCATGTACTCTGCGTTGATACCAC171990.9967123964039771No Hit
GAGTACATGGGAAGCAGTGGTATCA97160.5630593431862924No Hit
GTATCAACGCAGAGTACATGGGAAG65080.377150082899999No Hit
GCGTTGATACCACTGCTTCCCATGT59210.3431323971805308No Hit
CATGTACTCTGCGTTGATACCACTG55190.3198357878803157No Hit
TATCAACGCAGAGTACATGGGAAGC48660.28199328570857335No Hit
ACGCAGAGTACATGGGAAGCAGTGG47860.27735714455430166No Hit
GCTTCCCATGTACTCTGCGTTGATA41330.2395146423825593No Hit
ACTCTGCGTTGATACCACTGCTTCC40040.23203886477129626No Hit
GGTATCAACGCAGAGTACATGGGAA31940.18509793558429577No Hit
GTATCAACGCAGAGTACTTTTTTTT25030.14505326636427437No Hit
CAGTGGTATCAACGCAGAGTACATG23590.1367082122865854No Hit
GTGGTATCAACGCAGAGTACATGGG20680.11984424883792223No Hit
GTCCTACAGTGGACATTTCTAAATT20070.11630919120779011No Hit
CCACTGCTTCCCATGTACTCTGCGT19980.11578762532793453No Hit
GCAGTGGTATCAACGCAGAGTACAT19530.11317979592865675No Hit
CTGCTTCCCATGTACTCTGCGTTGA18710.10842775124552828No Hit
GGGAAGCAGTGGTATCAACGCAGAG18240.10570401831739369No Hit
ATACCACTGCTTCCCATGTACTCTG17980.10419727244225542No Hit
ACATGGGAAGCAGTGGTATCAACGC17680.10245871950940355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC653.3755823E-614.61415910
GCGTTTA2600.013.88184113
GTATCGA1102.746674E-912.95458518
TAGGACC5000.012.7326224
TCCAACG1352.7284841E-1112.66670518
TATCGAC1207.403287E-1012.66670519
GTAGGAC10450.012.5473123
TGTAGGA11850.012.0270972
GGTATCG951.04213E-611.99795117
CGGTTTC951.0424937E-611.99760313
CGTTTAT3100.011.94922514
AGGACCT8100.011.4951955
GTCCTAC9750.011.4145941
AGGACGT8000.011.4013575
CTGTAGG12300.011.2908581
TAGGACA4150.011.2191784
GCGTGCG855.327965E-511.1755329
CCGGTTT1201.2804048E-711.08015512
GGACCTG7650.011.0516816
GGACGTG8000.011.0431436
Produced by FastQC (version 0.11.5)