##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513098_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1725573
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.245658688447257	31.0	30.0	34.0	25.0	34.0
2	29.032388082103743	31.0	30.0	34.0	16.0	34.0
3	29.368978304597952	31.0	30.0	34.0	23.0	34.0
4	32.20047137965186	35.0	33.0	37.0	19.0	37.0
5	32.428350466772486	35.0	33.0	37.0	22.0	37.0
6	32.89791680792409	37.0	35.0	37.0	26.0	37.0
7	32.89741610467943	37.0	35.0	37.0	26.0	37.0
8	32.87481955269351	37.0	35.0	37.0	26.0	37.0
9	34.22993347716961	39.0	35.0	39.0	25.0	39.0
10	33.729959265704785	39.0	34.0	39.0	19.0	39.0
11	34.10144456363191	39.0	35.0	39.0	25.0	39.0
12	34.00159019641592	39.0	35.0	39.0	24.0	39.0
13	34.041837696811434	39.0	35.0	39.0	25.0	39.0
14	34.455553024995176	40.0	34.0	41.0	17.0	41.0
15	34.91695338302117	40.0	34.0	41.0	22.0	41.0
16	34.464220290883084	39.0	34.0	41.0	17.0	41.0
17	34.00205786715485	39.0	33.0	41.0	11.0	41.0
18	33.39217639589864	39.0	32.0	41.0	10.0	41.0
19	34.298040129278796	39.0	34.0	41.0	18.0	41.0
20	34.58965746450599	39.0	34.0	41.0	18.0	41.0
21	34.621959777998384	40.0	34.0	41.0	18.0	41.0
22	34.21798961852092	40.0	34.0	41.0	10.0	41.0
23	32.523914664867846	39.0	31.0	41.0	2.0	41.0
24	33.653993774821465	39.0	33.0	41.0	2.0	41.0
25	33.38391884898524	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.64769429787113
1101	2	1.0168229258058226
1101	3	0.7486794115262505
1101	4	0.778628636201006
1101	5	0.9674789489156765
1101	6	0.6526672356841061
1101	7	0.6610022620846792
1101	8	0.6710146447612928
1101	9	0.7432647934878673
1101	10	1.1488238662557535
1101	11	0.8227485655138835
1101	12	0.7991388965739219
1101	13	0.6799363696394494
1101	14	-0.14409889068206638
1101	15	0.3468642862027167
1101	16	1.0954731797335384
1101	17	1.7188802360445052
1101	18	-4.363226437661069
1101	19	-0.9040184111894334
1101	20	0.21861634825714304
1101	21	0.5789785108412744
1101	22	1.1926977242048196
1101	23	2.9131459659400036
1101	24	1.8556269058748498
1101	25	2.0839975684614416
1102	1	0.8141823293109312
1102	2	1.0786307143017133
1102	3	0.8101299295684079
1102	4	1.3784857280195126
1102	5	1.223284593788847
1102	6	0.7773546240370877
1102	7	0.7249536733029771
1102	8	0.6063486926355282
1102	9	0.8007853365389508
1102	10	1.1481093253482868
1102	11	0.7216410271073102
1102	12	0.8895283213684948
1102	13	0.7799720966848227
1102	14	1.6933430528691957
1102	15	1.112137598099821
1102	16	1.3559233405052424
1102	17	0.9118062810605849
1102	18	1.3388100039252109
1102	19	1.1517157795929833
1102	20	1.0214030577962632
1102	21	1.0870170960502819
1102	22	1.3849092283134325
1102	23	3.0492660088124524
1102	24	1.9385136511410153
1102	25	2.246198354456439
1103	1	0.7552253393758797
1103	2	1.0289789076309006
1103	3	0.7690571361980041
1103	4	1.2583029192535307
1103	5	1.127282081912675
1103	6	0.7028250355183303
1103	7	0.6202155435007413
1103	8	0.579423680021236
1103	9	0.7251858748350486
1103	10	1.056180601126833
1103	11	0.8993185365769136
1103	12	0.9930545742862549
1103	13	0.8263085885990762
1103	14	1.5890521248898821
1103	15	1.11778647683267
1103	16	1.2327450160326094
1103	17	1.7668565541172896
1103	18	1.724662223169105
1103	19	1.231601582259529
1103	20	1.0847155169734322
1103	21	0.965964692292026
1103	22	1.3142421662588504
1103	23	2.7697155106043
1103	24	1.6716228021100719
1103	25	1.8361663551696878
1104	1	0.27163437342455055
1104	2	0.6185701791234557
1104	3	0.4662232093604253
1104	4	0.8551576344002569
1104	5	0.7792637213139386
1104	6	0.2731672097317954
1104	7	0.1791364843887422
1104	8	0.15748326311726402
1104	9	0.282196096403581
1104	10	0.6208755041494527
1104	11	0.10682858651820482
1104	12	0.33026291185434076
1104	13	0.16803676301581305
1104	14	1.0787518699099792
1104	15	0.4023817657449129
1104	16	0.8149469199763288
1104	17	1.3388910943384644
1104	18	1.8764304311570257
1104	19	0.9950346895051396
1104	20	0.3791914210846201
1104	21	0.5205032259626066
1104	22	0.8280696965461019
1104	23	2.3600878937683376
1104	24	1.3871506245791139
1104	25	1.4406565327862992
1105	1	0.622306359621458
1105	2	0.980003832073546
1105	3	0.6580478922773665
1105	4	0.891933213010411
1105	5	0.9446571119459293
1105	6	0.6261877487544609
1105	7	0.6320151363129867
1105	8	0.49477225549639314
1105	9	0.7968875908405622
1105	10	1.140799147137784
1105	11	0.8026367373634713
1105	12	0.8692151225987033
1105	13	0.8138640710912668
1105	14	1.4002150126350514
1105	15	0.7996451245141003
1105	16	1.2095625023437648
1105	17	1.7322911195518529
1105	18	1.7458568513257546
1105	19	1.3374115950315684
1105	20	0.9982583033010286
1105	21	0.8996076316311701
1105	22	1.4072257649386515
1105	23	2.8331963335787904
1105	24	1.7895023908757324
1105	25	1.665655853013618
1106	1	-0.891795204050922
1106	2	-0.25572152983207275
1106	3	-0.5638360178039683
1106	4	-2.784014444482313
1106	5	-0.7428049967608104
1106	6	-0.7255299898731522
1106	7	-0.9114155388773426
1106	8	-0.5952664611517662
1106	9	-0.5744260740353297
1106	10	-0.31596276048249194
1106	11	-0.43564422344967824
1106	12	-0.4776690375901822
1106	13	-0.6730978225229904
1106	14	0.10570637392040538
1106	15	-0.5084668636408054
1106	16	-1.3170944601501091
1106	17	-6.086914138709197
1106	18	-7.077460675210652
1106	19	-3.2115799713419726
1106	20	-1.9994547132961102
1106	21	-1.6168574483532225
1106	22	-2.015401964985216
1106	23	-5.719414789204002
1106	24	-2.7740469353345354
1106	25	-1.282132195847396
1107	1	0.5730695095150402
1107	2	1.0066903629339876
1107	3	0.5959001269991688
1107	4	0.9378638910280443
1107	5	0.9819134208544185
1107	6	0.5453510202655565
1107	7	0.5133492804297433
1107	8	0.5254691238454328
1107	9	0.39915332510629753
1107	10	0.9974382471681906
1107	11	0.5823351259314222
1107	12	0.5800755665026216
1107	13	0.49574549401616963
1107	14	1.3263220360464203
1107	15	0.6924234765968009
1107	16	-5.667312535661708
1107	17	-1.0472552471249443
1107	18	0.6419815092765049
1107	19	0.6303121727147527
1107	20	0.4100631071927836
1107	21	0.5552519004058709
1107	22	0.7341877594658612
1107	23	2.504291499282658
1107	24	1.5379657535999698
1107	25	1.1288799332811763
1108	1	0.23092287756031027
1108	2	0.4940139166465549
1108	3	0.22221456103349624
1108	4	0.7913571487018274
1108	5	0.6065922366992709
1108	6	0.18447141560750424
1108	7	0.07515288776913565
1108	8	0.13997368368455199
1108	9	0.33240825518876704
1108	10	0.773488132587616
1108	11	0.2320156409983838
1108	12	0.3014037657948734
1108	13	0.2487380891492208
1108	14	0.6438245225227988
1108	15	0.45286895339229005
1108	16	0.6780725330086881
1108	17	0.781147390566602
1108	18	0.7142219569981378
1108	19	0.5913298352710683
1108	20	0.5289704917937783
1108	21	0.4705079217318868
1108	22	0.8276364944028032
1108	23	2.4049113879758153
1108	24	1.2492350569189057
1108	25	1.4089661103432363
1109	1	0.21512039971142016
1109	2	0.5259708703077202
1109	3	0.380253454728976
1109	4	0.42372558583969067
1109	5	0.06553166796987142
1109	6	0.09100518088295928
1109	7	0.17302193268006505
1109	8	0.09116821454612989
1109	9	0.29442221790067435
1109	10	0.715548738495464
1109	11	0.35777456965420384
1109	12	0.3715969235745291
1109	13	0.26925630627260233
1109	14	-0.8621954240284637
1109	15	-0.09481454586823901
1109	16	-0.4002737949266262
1109	17	0.6704970261233143
1109	18	1.4518820212349368
1109	19	0.5254294646838815
1109	20	0.44116206035241134
1109	21	0.36464198216639687
1109	22	0.17488280034612558
1109	23	-4.414043948107885
1109	24	-1.5731802691707202
1109	25	-0.28997480806520315
1110	1	0.5348080341920962
1110	2	0.8244938248394007
1110	3	0.6302660955300539
1110	4	1.0916210730431004
1110	5	0.842398451066579
1110	6	0.40764690262740544
1110	7	0.44105245914341396
1110	8	0.3657298419712234
1110	9	0.4507980481992959
1110	10	0.7946064509450892
1110	11	0.4828634072453113
1110	12	0.5967423846087243
1110	13	0.46468260122014016
1110	14	1.3233575788810654
1110	15	0.8136498936912844
1110	16	1.2159674001279726
1110	17	1.487262584868148
1110	18	2.04637797770895
1110	19	1.2909000605889958
1110	20	0.8175158656620383
1110	21	0.7316696309345474
1110	22	1.0538337546500856
1110	23	2.381399854196104
1110	24	1.5232108280324468
1110	25	1.771831669534258
1111	1	0.5306203784617907
1111	2	0.8561360924163317
1111	3	0.5737518185076098
1111	4	1.0804245936070558
1111	5	0.8670048921884472
1111	6	0.2939323796569866
1111	7	0.2852604685056903
1111	8	0.025778233870795475
1111	9	0.2091760847080053
1111	10	0.39290450976946545
1111	11	0.19579990421085114
1111	12	0.32372576131986364
1111	13	0.21147819238959897
1111	14	-0.4516998577008735
1111	15	0.020356063646097766
1111	16	0.5057397248367792
1111	17	0.945529588053482
1111	18	1.638194385768557
1111	19	0.6683200890979535
1111	20	0.5792667685200215
1111	21	0.42360220036258056
1111	22	0.7755577885338312
1111	23	0.6276218227411263
1111	24	0.65285127527725
1111	25	0.18512187774279454
1112	1	0.530756703247679
1112	2	0.8236611797509923
1112	3	0.5892739526491582
1112	4	1.0992854085535626
1112	5	0.8822431440043275
1112	6	0.3403062935262682
1112	7	0.24628077017661099
1112	8	0.36893452538730287
1112	9	0.44027033874592547
1112	10	0.6020346453079597
1112	11	0.4386780829525563
1112	12	0.32242020896961776
1112	13	0.3297225844372136
1112	14	0.6907804773211197
1112	15	0.43162420071428187
1112	16	0.8026987630679656
1112	17	1.1580984780225307
1112	18	1.258096119595173
1112	19	0.7422788383410222
1112	20	0.5383762255797535
1112	21	0.7031415349138186
1112	22	0.7727398990457459
1112	23	2.3133232418464047
1112	24	1.4705605287177903
1112	25	1.4234227092729341
1113	1	0.341724233565035
1113	2	0.7838674557778624
1113	3	0.4603199865842207
1113	4	0.6483423722798136
1113	5	0.7798981200327049
1113	6	0.2758558214493263
1113	7	0.22426965948713473
1113	8	0.11461674844009195
1113	9	0.2547594076551789
1113	10	0.3882510797091996
1113	11	0.2116876430110466
1113	12	0.2702547057526914
1113	13	0.26235330813951663
1113	14	0.8731415430199405
1113	15	0.42944718342550203
1113	16	0.7164063825764941
1113	17	1.1582913322283872
1113	18	1.766032512015535
1113	19	0.8266286415621522
1113	20	0.49741342045035
1113	21	0.5682831631959147
1113	22	0.8582385103395183
1113	23	0.5455771461044279
1113	24	0.6690449337781601
1113	25	0.9529504555870005
1114	1	-0.13000454191224264
1114	2	-2.872558631369749
1114	3	-0.7980680125432009
1114	4	0.3453008298495277
1114	5	0.522187871341977
1114	6	0.4000610257365409
1114	7	0.30428172982310997
1114	8	0.42149190889998067
1114	9	0.43218959453635364
1114	10	0.5664514740854472
1114	11	0.3971101396088983
1114	12	0.5575112823974493
1114	13	0.39790955928183536
1114	14	1.2958450302857898
1114	15	0.6532951014801398
1114	16	0.8934876445590589
1114	17	1.3260719501202516
1114	18	1.4361942341501859
1114	19	0.9372860728853212
1114	20	0.8324223258070163
1114	21	0.6945642294375105
1114	22	0.8815537685412664
1114	23	2.446816392276574
1114	24	1.3416740534100953
1114	25	1.3530839689391385
1115	1	0.5643264085380579
1115	2	0.941480286653448
1115	3	0.6175814621557656
1115	4	0.8911701829370315
1115	5	0.8620569534837088
1115	6	0.3287679271967079
1115	7	0.24306270942953034
1115	8	0.24957779478469178
1115	9	0.26578765629235335
1115	10	0.47812216894356396
1115	11	0.49161327806415045
1115	12	0.3622565639160058
1115	13	0.35413414584409963
1115	14	1.2046295636013227
1115	15	0.6107340356030164
1115	16	0.9768348851333855
1115	17	1.470911120240821
1115	18	1.9694970042762563
1115	19	1.1389600465800171
1115	20	0.808704753402651
1115	21	0.8380192200427601
1115	22	1.0769370880982478
1115	23	2.709106630178745
1115	24	1.499950563392872
1115	25	1.2315520258063373
1116	1	0.6071797800398997
1116	2	0.5197924794451296
1116	3	0.727796626955012
1116	4	1.4129389844901112
1116	5	0.7893990998158813
1116	6	0.5089201821977909
1116	7	0.457710294235639
1116	8	0.4606677344250443
1116	9	0.616529086785377
1116	10	1.025965402683191
1116	11	0.6829682541878768
1116	12	0.6320234043643325
1116	13	0.48731147676615905
1116	14	1.4312107941047643
1116	15	0.8043697837411301
1116	16	1.1196848444566285
1116	17	1.4100522210096358
1116	18	1.9505665965568326
1116	19	0.9911552447467784
1116	20	0.7499452419547907
1116	21	0.5465239854195616
1116	22	0.9870154065766314
1116	23	2.3060798481684657
1116	24	1.3722201470532056
1116	25	1.6042319688409137
1201	1	0.9105157537132023
1201	2	1.3082120073405434
1201	3	1.0105177898453057
1201	4	1.4949500581841164
1201	5	1.2462339664639615
1201	6	0.9077174753822277
1201	7	0.8548284885381037
1201	8	0.7821876360490307
1201	9	1.0733464986720236
1201	10	1.2867501097554168
1201	11	0.903360922401383
1201	12	1.0579171250261936
1201	13	0.9202217656842535
1201	14	1.7207641439684949
1201	15	1.2983572330301314
1201	16	1.5115636602272744
1201	17	2.0896078768686124
1201	18	2.6774475191909346
1201	19	1.7970956654999526
1201	20	1.4906205888005672
1201	21	1.4713677161363492
1201	22	1.6862703586891286
1201	23	3.2517087579735673
1201	24	2.063135656665864
1201	25	2.1626169166413476
1202	1	0.8258762884341024
1202	2	1.1706504023317663
1202	3	0.9008696875263489
1202	4	1.4557590491663461
1202	5	1.2620767652879152
1202	6	0.9958399591833711
1202	7	0.6240080050048888
1202	8	0.7216684265673194
1202	9	0.8124550151458507
1202	10	1.3011933555902502
1202	11	0.9936232768937003
1202	12	0.9567333217673877
1202	13	0.9354194305107058
1202	14	1.7209615620223744
1202	15	1.2015800827597403
1202	16	1.4035513074976151
1202	17	1.752678714696728
1202	18	2.327894177537843
1202	19	1.5693175351899313
1202	20	1.3227658884882203
1202	21	1.3249892798283511
1202	22	1.7216152409786076
1202	23	3.23395170909145
1202	24	1.916249573933598
1202	25	2.164834344425117
1203	1	0.8343825904207876
1203	2	1.2786142603826818
1203	3	1.0132986673272804
1203	4	1.5897655815027463
1203	5	1.3612507048666416
1203	6	0.9976177534699744
1203	7	0.9076208792071085
1203	8	0.7594003820326947
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2211	17	0.1814770898315743
2211	18	-5.327362117205713
2211	19	-1.7321415081152836
2211	20	-0.9553474659297407
2211	21	-0.6062162320051954
2211	22	-0.2318531249214928
2211	23	-4.660968876211417
2211	24	-2.131274488494892
2211	25	-1.4505810350982742
2212	1	0.2764586494984904
2212	2	0.4806713929168005
2212	3	0.35871596505378633
2212	4	0.8136391790794804
2212	5	0.6616087547394471
2212	6	0.22545363660014317
2212	7	0.12108382750466973
2212	8	0.0656007681257833
2212	9	-0.0052302766506571174
2212	10	-3.8475656359561334
2212	11	-1.1503160931149807
2212	12	-0.37887280704120485
2212	13	-0.14480756473995626
2212	14	0.6971065544753472
2212	15	0.18106451039908222
2212	16	0.2827448836520574
2212	17	0.9489837241653944
2212	18	1.4592567018412765
2212	19	0.7850833230107597
2212	20	0.37665830739648953
2212	21	0.36590604770996293
2212	22	0.5158149795307949
2212	23	1.898642380942853
2212	24	0.8709929354702339
2212	25	0.8676943708647258
2213	1	-1.4583180196784191
2213	2	-3.881972528578313
2213	3	-1.7087947573467375
2213	4	-3.4506029483999257
2213	5	-1.1928432096603494
2213	6	-0.9657525405368226
2213	7	-0.9581900227663418
2213	8	-0.9125369547929516
2213	9	-0.7700240111703778
2213	10	-0.8080091086241126
2213	11	-0.9762170063909323
2213	12	-0.7923933204666938
2213	13	-1.1553311928059813
2213	14	-5.883503219096426
2213	15	-2.6496990559559066
2213	16	-1.8646284648935634
2213	17	-0.7193202565628312
2213	18	-0.045702913412817736
2213	19	-0.6168356661693366
2213	20	-0.8964022605648339
2213	21	-1.021480735483685
2213	22	-0.8918724510469858
2213	23	-5.410355331827176
2213	24	-3.0227165895551664
2213	25	-2.1654020515542243
2214	1	0.03360467528976585
2214	2	0.1480662139742357
2214	3	-0.08429417934365446
2214	4	0.31371569127504273
2214	5	0.12679463938571445
2214	6	-0.1482905769984484
2214	7	-0.1824608465533757
2214	8	-0.25829641654532765
2214	9	-0.12733174408525372
2214	10	0.2913583839679603
2214	11	-0.2115693042278579
2214	12	-0.08610195653483999
2214	13	-0.17699867141022452
2214	14	0.5427356020238108
2214	15	0.0856586012049263
2214	16	0.5247553575584689
2214	17	0.7477638478432311
2214	18	1.2179894892632035
2214	19	0.2801364230076686
2214	20	0.14766290452645592
2214	21	0.15101794746246355
2214	22	0.4852594210130903
2214	23	1.8934915372825927
2214	24	0.8357061506993091
2214	25	0.6331554328220079
2215	1	-2.285625656143459
2215	2	-4.241792909044346
2215	3	-2.2467937412848933
2215	4	-3.54695961739678
2215	5	-4.887371433920734
2215	6	-2.3009389104870834
2215	7	-1.5971924707014047
2215	8	-1.9783192743836473
2215	9	-1.484773567844094
2215	10	-2.9673171490986157
2215	11	-1.9159081642101512
2215	12	-1.5300527113949798
2215	13	-1.5075390688497308
2215	14	-0.5920969834628451
2215	15	-1.3553234056502461
2215	16	-1.9535901413571821
2215	17	-6.701252360466821
2215	18	-2.669066736835429
2215	19	-1.8549285713294879
2215	20	-1.5361703229030539
2215	21	-1.5741691138165947
2215	22	-1.3655410244077757
2215	23	-6.09450103432745
2215	24	-3.302775566154178
2215	25	-3.0509596463695097
2216	1	-0.5792682693259366
2216	2	-0.1429961390694814
2216	3	-0.3711678713262927
2216	4	-2.8866217258608984
2216	5	-0.9107906040244806
2216	6	-0.6027317499036897
2216	7	-0.6461574476367602
2216	8	-0.4028167442246584
2216	9	-0.3923679953888879
2216	10	-0.17004001370137445
2216	11	-0.5335306715687267
2216	12	-1.2064671043902635
2216	13	-0.5462084561064344
2216	14	-6.288373496517423
2216	15	-2.42237659516924
2216	16	-0.9492581130793241
2216	17	0.007866575732535352
2216	18	0.7877230319127051
2216	19	0.190398291965991
2216	20	-0.4992643253770339
2216	21	-0.4764146267157088
2216	22	-0.2082314738335853
2216	23	0.7995221673049002
2216	24	0.22081701847647395
2216	25	0.40955596940341366
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83871.0
3	2.0
4	748.0
5	4199.0
6	5553.0
7	4781.0
8	4539.0
9	4146.0
10	3785.0
11	3800.0
12	3814.0
13	3646.0
14	3615.0
15	3591.0
16	3746.0
17	3912.0
18	4421.0
19	5168.0
20	6403.0
21	8133.0
22	9982.0
23	12162.0
24	14199.0
25	16907.0
26	19314.0
27	22100.0
28	24402.0
29	29991.0
30	35140.0
31	39544.0
32	51903.0
33	68077.0
34	89114.0
35	118170.0
36	170052.0
37	242076.0
38	600567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60198512864931	18.207629210595474	12.293317290483298	24.897068370271917
2	13.131570815072413	20.14364838844899	42.19897528187865	24.525805514599945
3	20.057520040997957	25.338383873518524	28.42905707017654	26.175039015306982
4	10.688884161049781	16.776221777650758	38.726335603275814	33.80855845802365
5	11.844765895477915	38.817337949489726	36.19101008953272	13.146886065499642
6	32.1306293069121	35.390333969172524	18.8475603076372	13.63147641627818
7	28.01152984968651	31.45367570471946	21.81043911616851	18.724355329425517
8	25.80313830098638	36.54124939644714	20.70187763628702	16.953734666279466
9	27.424052977276325	15.31896097482084	19.803468402432994	37.453517645469844
10	14.23465862187306	30.10977340394943	34.64598425050782	21.009583723669685
11	33.28400720590383	23.818723293756506	22.857762228358524	20.039507271981137
12	23.73273732447512	26.117429665428975	31.0801192172283	19.069713792867603
13	28.901282145653738	21.71092631995043	24.875051512451957	24.51274002194387
14	20.67644157256349	23.25748275306422	28.0736989880793	27.992376686292992
15	25.722263086173086	28.820851859773466	22.881841741163676	22.575043312889765
16	20.835963434207482	28.36636971885888	29.820388020303508	20.977278826630126
17	22.615491717613427	28.474808525783303	28.44397093150982	20.465728825093453
18	22.038643338849397	26.851179384036655	30.736785363407943	20.37339191370601
19	22.972659628490007	27.966549724416662	28.775561311206282	20.28522933588705
20	23.334185404997775	27.496700395952484	28.34836631198459	20.820747887065146
21	21.869103729153107	27.41817026705705	28.864316283286485	21.848409720503355
22	22.22103057785219	28.30686143675209	26.390716613699684	23.081391371696036
23	20.041573582110143	28.165754080552585	29.503968967048355	22.288703370288918
24	21.900687740844084	29.572740348409752	27.43473186919068	21.09184004155548
25	22.076289166571986	27.0547595804919	26.99789225700088	23.871058995935233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	177.0
2	328.0
3	479.0
4	479.0
5	479.0
6	1028.5
7	1578.0
8	1578.0
9	1578.0
10	2404.0
11	3230.0
12	3230.0
13	3230.0
14	5501.5
15	7773.0
16	7773.0
17	7773.0
18	13453.5
19	19134.0
20	19134.0
21	19134.0
22	30274.0
23	41414.0
24	41414.0
25	41414.0
26	58397.5
27	75381.0
28	75381.0
29	75381.0
30	96724.5
31	118068.0
32	118068.0
33	118068.0
34	136537.0
35	155006.0
36	155006.0
37	155006.0
38	170613.5
39	186221.0
40	186221.0
41	186221.0
42	202381.0
43	218541.0
44	218541.0
45	218541.0
46	257599.5
47	296658.0
48	296658.0
49	296658.0
50	269058.0
51	241458.0
52	241458.0
53	241458.0
54	198495.5
55	155533.0
56	155533.0
57	155533.0
58	126613.5
59	97694.0
60	97694.0
61	97694.0
62	77649.5
63	57605.0
64	57605.0
65	57605.0
66	43394.0
67	29183.0
68	29183.0
69	29183.0
70	20924.0
71	12665.0
72	12665.0
73	12665.0
74	8777.0
75	4889.0
76	4889.0
77	4889.0
78	3377.5
79	1866.0
80	1866.0
81	1866.0
82	1274.0
83	682.0
84	682.0
85	682.0
86	460.5
87	239.0
88	239.0
89	239.0
90	155.5
91	72.0
92	72.0
93	72.0
94	44.5
95	17.0
96	17.0
97	17.0
98	13.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10755847477910237
2	0.04670912212928691
3	0.03529262453689296
4	0.03280069866647195
5	0.035756238652320124
6	0.017037818741948325
7	0.01808095050165945
8	0.021558056367363188
9	0.026426004579348423
10	0.032742746902043554
11	0.01860251638151501
12	0.018486612852658218
13	0.015183362280239665
14	0.01964564814122613
15	0.019471792847940945
16	0.020514924607652064
17	0.02352841635792864
18	0.017849143443945868
19	0.02926564103633981
20	0.021210345780792814
21	0.02526696929078051
22	0.03471310689260901
23	0.0254408245840657
24	0.03766864687845719
25	0.02926564103633981
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1725573.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.53474055565682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64288370361265	65.18331455250417
2	8.370468596070195	12.310404730825896
3	1.6664099896471756	3.6761707874417953
4	0.5168074493430452	1.5201320681868633
5	0.23082641683112243	0.8486880337534243
6	0.13372789104818078	0.5900187463969869
7	0.07671649594872317	0.3948929338149919
8	0.06305214866534964	0.3709218714866551
9	0.04191695348951118	0.2774117069761265
>10	0.2196887212434689	2.923360474170842
>50	0.018563448899409675	0.9521689810308961
>100	0.014123717466681809	2.124761210451799
>500	0.0016048225781727793	0.8585832968515752
>1k	0.0027281983828937252	3.99364258681913
>5k	3.209645156345559E-4	1.632317251329941
>10k+	1.6048225781727796E-4	2.3432107679588863
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	22513	1.3046680725764717	No Hit
CCCATGTACTCTGCGTTGATACCAC	17199	0.9967123964039771	No Hit
GAGTACATGGGAAGCAGTGGTATCA	9716	0.5630593431862924	No Hit
GTATCAACGCAGAGTACATGGGAAG	6508	0.377150082899999	No Hit
GCGTTGATACCACTGCTTCCCATGT	5921	0.3431323971805308	No Hit
CATGTACTCTGCGTTGATACCACTG	5519	0.3198357878803157	No Hit
TATCAACGCAGAGTACATGGGAAGC	4866	0.28199328570857335	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4786	0.27735714455430166	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4133	0.2395146423825593	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4004	0.23203886477129626	No Hit
GGTATCAACGCAGAGTACATGGGAA	3194	0.18509793558429577	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2503	0.14505326636427437	No Hit
CAGTGGTATCAACGCAGAGTACATG	2359	0.1367082122865854	No Hit
GTGGTATCAACGCAGAGTACATGGG	2068	0.11984424883792223	No Hit
GTCCTACAGTGGACATTTCTAAATT	2007	0.11630919120779011	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1998	0.11578762532793453	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1953	0.11317979592865675	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1871	0.10842775124552828	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1824	0.10570401831739369	No Hit
ATACCACTGCTTCCCATGTACTCTG	1798	0.10419727244225542	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1768	0.10245871950940355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.7951764428395666E-5	0.0
4	0.0	0.0	0.0	5.7951764428395666E-5	0.0
5	0.0	0.0	0.0	5.7951764428395666E-5	0.0
6	0.0	0.0	0.0	5.7951764428395666E-5	5.7951764428395666E-5
7	0.0	0.0	0.0	5.7951764428395666E-5	5.7951764428395666E-5
8	0.0	0.0	0.0	5.7951764428395666E-5	5.7951764428395666E-5
9	0.0	0.0	0.0	1.1590352885679133E-4	5.7951764428395666E-5
10	0.0	0.0	0.0	1.1590352885679133E-4	5.7951764428395666E-5
11	0.0	5.7951764428395666E-5	0.0	1.1590352885679133E-4	5.7951764428395666E-5
12	0.0	5.7951764428395666E-5	0.0	2.3180705771358266E-4	5.7951764428395666E-5
13	0.0	5.7951764428395666E-5	0.0	2.3180705771358266E-4	5.7951764428395666E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	65	3.3755823E-6	14.614159	10
GCGTTTA	260	0.0	13.881841	13
GTATCGA	110	2.746674E-9	12.954585	18
TAGGACC	500	0.0	12.732622	4
TCCAACG	135	2.7284841E-11	12.666705	18
TATCGAC	120	7.403287E-10	12.666705	19
GTAGGAC	1045	0.0	12.547312	3
TGTAGGA	1185	0.0	12.027097	2
GGTATCG	95	1.04213E-6	11.997951	17
CGGTTTC	95	1.0424937E-6	11.997603	13
CGTTTAT	310	0.0	11.949225	14
AGGACCT	810	0.0	11.495195	5
GTCCTAC	975	0.0	11.414594	1
AGGACGT	800	0.0	11.401357	5
CTGTAGG	1230	0.0	11.290858	1
TAGGACA	415	0.0	11.219178	4
GCGTGCG	85	5.327965E-5	11.175532	9
CCGGTTT	120	1.2804048E-7	11.080155	12
GGACCTG	765	0.0	11.051681	6
GGACGTG	800	0.0	11.043143	6
>>END_MODULE