FastQCFastQC Report
Thu 26 May 2016
SRR1513098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725573
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC247461.4340743625450794No Hit
CCCATGTACTCTGCGTTGATACCAC237631.3771077781119663No Hit
GAGTACATGGGAAGCAGTGGTATCA98850.5728531913746912No Hit
CATGTACTCTGCGTTGATACCACTG68630.39772295927207946No Hit
GTATCAACGCAGAGTACATGGGAAG65230.37801935936642495No Hit
ACGCAGAGTACATGGGAAGCAGTGG61690.3575044347587729No Hit
GCGTTGATACCACTGCTTCCCATGT61340.35547612300377907No Hit
TATCAACGCAGAGTACATGGGAAGC58270.3376849313242616No Hit
GCTTCCCATGTACTCTGCGTTGATA51230.296886889166671No Hit
ACTCTGCGTTGATACCACTGCTTCC49820.28871569038226724No Hit
GTATCAACGCAGAGTACTTTTTTTT40580.23516826005042962No Hit
GGTATCAACGCAGAGTACATGGGAA38870.22525850833317396No Hit
TATCAACGCAGAGTACTTTTTTTTT29190.16916120036648696No Hit
CAGTGGTATCAACGCAGAGTACATG27410.15884578629823254No Hit
CCACTGCTTCCCATGTACTCTGCGT25640.1485883239944065No Hit
ACATGGGAAGCAGTGGTATCAACGC24300.14082278756100147No Hit
GGTATCAACGCAGAGTACTTTTTTT24240.1404750769744311No Hit
ATACCACTGCTTCCCATGTACTCTG24120.13977965580129034No Hit
CATGGGAAGCAGTGGTATCAACGCA23590.1367082122865854No Hit
GTGGTATCAACGCAGAGTACATGGG22190.12859496526661No Hit
GCAGTGGTATCAACGCAGAGTACAT21810.12639279821833094No Hit
TACCACTGCTTCCCATGTACTCTGC21180.12274183705934202No Hit
CTGCTTCCCATGTACTCTGCGTTGA20780.1204237664822062No Hit
GGGAAGCAGTGGTATCAACGCAGAG20750.12024991118892102No Hit
GTCCTACAGTGGACATTTCTAAATT19570.11341160298637032No Hit
AAGCAGTGGTATCAACGCAGAGTAC18820.10906522065424065No Hit
GTACTCTGCGTTGATACCACTGCTT18130.10506654890868135No Hit
CTTCCCATGTACTCTGCGTTGATAC17950.10402341714897023No Hit
GATACCACTGCTTCCCATGTACTCT17410.10089402186983686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCG307.7928137E-418.9746657
GGAGTCG400.005203623314.2810896
TCCAACG1205.2750693E-1113.44389418
TAGGACC5000.012.9481874
ACCGTGG751.4992302E-512.6497778
GGACCGT550.003018992312.1172886
ATCCGTG658.114521E-411.6767177
CCAACGA907.580753E-611.59462119
TGTACCG752.0325002E-411.42487055
ACAGCGC855.396248E-511.1615688
GTATTAG1552.9649527E-1011.0659841
GGCGAGA6200.011.0165919
AGGACCT8200.010.9140025
GTAGGAC11350.010.9047963
TGGCGAG9350.010.85999618
GGACCTG6950.010.8221216
AAAGGCG1158.572697E-710.7625615
TCTAGAC2050.010.6817893
CTAGACA2350.010.5335694
TAGACAG3350.010.5154295
Produced by FastQC (version 0.11.5)