FastQCFastQC Report
Thu 26 May 2016
SRR1513097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866355
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC263571.4122179328155684No Hit
CCCATGTACTCTGCGTTGATACCAC208991.1197762483557523No Hit
GAGTACATGGGAAGCAGTGGTATCA117000.6268903825906647No Hit
GTATCAACGCAGAGTACATGGGAAG72520.3885648764570513No Hit
GCGTTGATACCACTGCTTCCCATGT72470.3882969745841493No Hit
CATGTACTCTGCGTTGATACCACTG66820.3580240629462241No Hit
TATCAACGCAGAGTACATGGGAAGC58740.3147311202852619No Hit
ACGCAGAGTACATGGGAAGCAGTGG54240.2906199517240825No Hit
GCTTCCCATGTACTCTGCGTTGATA48040.2574001194842353No Hit
ACTCTGCGTTGATACCACTGCTTCC45780.2452909548290652No Hit
GGTATCAACGCAGAGTACATGGGAA36670.196479233586322No Hit
CAGTGGTATCAACGCAGAGTACATG25710.13775514304620504No Hit
GTATCAACGCAGAGTACTTTTTTTT25230.13518328506634592No Hit
CCACTGCTTCCCATGTACTCTGCGT24320.1303074709795296No Hit
GCAGTGGTATCAACGCAGAGTACAT22910.12275263816369342No Hit
GTGGTATCAACGCAGAGTACATGGG22570.12093090542795984No Hit
CTGCTTCCCATGTACTCTGCGTTGA22180.1188412708193243No Hit
ATACCACTGCTTCCCATGTACTCTG22050.11814472594977911No Hit
GGGAAGCAGTGGTATCAACGCAGAG21740.11648373433778675No Hit
GTACTCTGCGTTGATACCACTGCTT20990.11246520624425685No Hit
CCCATGTACTCTGCGTTTATACCAC19720.10566049867254622No Hit
CATGGGAAGCAGTGGTATCAACGCA19390.10389234631139306No Hit
AAGCAGTGGTATCAACGCAGAGTAC19160.10265999769604389No Hit
GTACATGGGAAGCAGTGTTATCAAC18880.10115974720779272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGA350.002171318516.28470418
TATCGAC1200.015.83235119
GTATCGA1051.373337E-913.57058618
GCGTTTA3350.013.32574513
GTCTAGA1900.012.5176771
GTAGGAC8700.012.339973
CGTTTAT3650.012.23047814
GCACCGT1103.8186045E-812.0901586
TGTAGGA9300.012.0543072
TAGGACC3450.011.8423844
TGCGTTT3950.011.782512
GTCCTAA4500.011.6275311
GGCCTAT907.346209E-611.6275311
TGTACCG907.4522704E-611.6125695
GGTATCG1157.089875E-811.56356917
GGACGTG7400.011.4249666
AGGACGT7500.011.2747495
AGCACCG1104.9615664E-711.2286825
TAGGACG8200.011.1236464
CGTGAAA5100.010.9892199
Produced by FastQC (version 0.11.5)