##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513097_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1866355
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.196402613650672	31.0	30.0	34.0	24.0	34.0
2	28.924363800027326	31.0	30.0	34.0	16.0	34.0
3	29.273256695537558	31.0	30.0	34.0	23.0	34.0
4	32.10297719351356	35.0	32.0	37.0	19.0	37.0
5	32.33420865805273	35.0	33.0	37.0	22.0	37.0
6	32.79749565329211	36.0	33.0	37.0	26.0	37.0
7	32.79858654971857	37.0	35.0	37.0	26.0	37.0
8	32.770575801495426	37.0	35.0	37.0	25.0	37.0
9	34.111332517125625	39.0	35.0	39.0	25.0	39.0
10	33.614581363138306	38.0	34.0	39.0	18.0	39.0
11	33.981734450305545	39.0	34.0	39.0	25.0	39.0
12	33.82195938071803	39.0	34.0	39.0	22.0	39.0
13	33.893681534327605	39.0	34.0	39.0	23.0	39.0
14	34.179149197232036	39.0	33.0	41.0	16.0	41.0
15	34.72021400001607	40.0	34.0	41.0	19.0	41.0
16	34.2828470467837	39.0	34.0	41.0	17.0	41.0
17	33.845585646889255	39.0	33.0	41.0	11.0	41.0
18	33.24949915744861	39.0	32.0	41.0	10.0	41.0
19	34.12743877772449	39.0	33.0	41.0	18.0	41.0
20	34.409537842479054	39.0	34.0	41.0	18.0	41.0
21	34.42721668707186	39.0	34.0	41.0	18.0	41.0
22	34.02072756790643	39.0	34.0	41.0	10.0	41.0
23	32.38227346887382	39.0	31.0	41.0	2.0	41.0
24	33.45684395519609	39.0	33.0	41.0	2.0	41.0
25	33.180041846272545	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7133687429706121
1101	2	1.1393108254538262
1101	3	0.8995332545871975
1101	4	0.8492842280293331
1101	5	1.0265059331962192
1101	6	0.7584864561305409
1101	7	0.835210830761298
1101	8	0.8341912413505881
1101	9	0.9502139763324919
1101	10	1.2022315333766755
1101	11	0.9087827677790514
1101	12	0.9303794190846872
1101	13	0.8633141582966104
1101	14	-0.0757904717197988
1101	15	0.5484153747982461
1101	16	1.1842866722520284
1101	17	1.904180306777505
1101	18	-4.17336368977298
1101	19	-0.6553119507042808
1101	20	0.2759675235275836
1101	21	0.7294605377461281
1101	22	1.2790480002327058
1101	23	3.0717634031854644
1101	24	1.9443044584415787
1101	25	2.1353773163785235
1102	1	0.9306026342241296
1102	2	1.1708743355402156
1102	3	0.9770138901599665
1102	4	1.4902317714571964
1102	5	1.1685384806419776
1102	6	0.933136375638874
1102	7	0.7632539909563434
1102	8	0.6961414362462719
1102	9	0.8871675815683275
1102	10	1.156915468401003
1102	11	0.7729129055950068
1102	12	0.9083358209905015
1102	13	0.746341592715865
1102	14	1.6079833017900569
1102	15	1.064094724109161
1102	16	1.4767199967586038
1102	17	1.2068610825085742
1102	18	1.5744484385047244
1102	19	1.229444153613528
1102	20	1.1233925352106269
1102	21	1.2053529674023693
1102	22	1.4230266387026376
1102	23	3.0621044028106184
1102	24	2.083514352363764
1102	25	2.232285236430222
1103	1	0.9052009069672309
1103	2	1.1544127900918646
1103	3	0.9338230063221573
1103	4	1.3707577446476265
1103	5	1.3266407687653796
1103	6	0.9192706354237572
1103	7	0.7687547970600264
1103	8	0.8440894671582626
1103	9	1.0731447835938042
1103	10	1.401850261046917
1103	11	1.0710539472474778
1103	12	1.1596535923598168
1103	13	1.0660748594415708
1103	14	1.8327670162249916
1103	15	1.280813238759059
1103	16	1.4981324569264842
1103	17	1.9053340269441819
1103	18	1.9194199410365655
1103	19	1.3138632669751757
1103	20	1.2448212688330997
1103	21	1.4186805482587275
1103	22	1.6932628687108888
1103	23	3.1367537666320757
1103	24	2.094651450541889
1103	25	2.318927757831702
1104	1	0.7042687605535178
1104	2	1.0446411760732737
1104	3	0.884909330965904
1104	4	1.5013818379027057
1104	5	1.3320673369601863
1104	6	0.8540006718704802
1104	7	0.7515108466599187
1104	8	0.8132687334720927
1104	9	0.8808605193778121
1104	10	1.1305600167376113
1104	11	0.8071336750849909
1104	12	0.9119162210733691
1104	13	0.7632799367076117
1104	14	1.5604086453581019
1104	15	1.020389526652778
1104	16	1.4340370281846262
1104	17	1.9163142971857638
1104	18	2.4042932874118605
1104	19	1.6493257763901568
1104	20	1.1287720733401585
1104	21	1.1257252296152984
1104	22	1.4681944995121583
1104	23	2.9578612718261112
1104	24	1.9103523548856742
1104	25	2.0065523009839126
1105	1	0.6832113388783689
1105	2	1.0175089663086077
1105	3	0.7313482417906556
1105	4	1.1647368808253447
1105	5	1.063869182589542
1105	6	0.7617300147625699
1105	7	0.5975982808680484
1105	8	0.5844080532215301
1105	9	0.7617878846482498
1105	10	1.2481240424270794
1105	11	0.8006685669330196
1105	12	0.7057172345227514
1105	13	0.75059585460086
1105	14	1.5160515672400408
1105	15	0.9029153814285777
1105	16	1.2642643943350151
1105	17	1.688877499494751
1105	18	1.6598754928396389
1105	19	1.3372037300319448
1105	20	1.0504274829499494
1105	21	0.8130743341418807
1105	22	1.403570947813293
1105	23	2.8455364601665423
1105	24	1.8944788361848808
1105	25	1.9453546406415683
1106	1	-0.9174941284937645
1106	2	-0.38140281274501575
1106	3	-0.650928766727489
1106	4	-3.1161216879764844
1106	5	-0.9368344085395819
1106	6	-0.8587435489734041
1106	7	-0.8670608057768554
1106	8	-0.8264779087282577
1106	9	-0.687848124207612
1106	10	-0.6563057673218751
1106	11	-0.8289460527525208
1106	12	-0.7077222941650021
1106	13	-0.7559109561612658
1106	14	-0.07219752278210478
1106	15	-0.6507470449288846
1106	16	-1.5770448480380566
1106	17	-6.115587958871419
1106	18	-7.171005590878327
1106	19	-3.3386190025283717
1106	20	-1.9693631283300235
1106	21	-1.6164258347199905
1106	22	-1.9021272022110978
1106	23	-5.380976729821825
1106	24	-2.598965570734702
1106	25	-1.0558949372037603
1107	1	0.42199936911061897
1107	2	0.7535120663089714
1107	3	0.531253358602001
1107	4	0.8037248176229994
1107	5	0.7939540342349645
1107	6	0.40418915523677157
1107	7	0.32393778160864173
1107	8	0.3094083248337114
1107	9	0.24523982196740235
1107	10	0.6723724915215286
1107	11	0.4548700452275867
1107	12	0.5189331589638684
1107	13	0.41905081586896387
1107	14	1.3239236125521927
1107	15	0.7677691273227083
1107	16	-5.617715767111264
1107	17	-0.8725745754412415
1107	18	0.7267786431787897
1107	19	0.5194612980526898
1107	20	0.45203434061430414
1107	21	0.5016796599135276
1107	22	0.6140428649244996
1107	23	2.5900435564874655
1107	24	1.479481135834682
1107	25	1.1027256520166802
1108	1	0.5114934050026818
1108	2	0.724213187215323
1108	3	0.4724539219619288
1108	4	1.015268169758123
1108	5	0.7740541366053222
1108	6	0.4376969655518437
1108	7	0.4069144694455673
1108	8	0.387301184531303
1108	9	0.5704867584525672
1108	10	0.9022960953408301
1108	11	0.4243512866194692
1108	12	0.5873061018974752
1108	13	0.40649890400268873
1108	14	0.9763666850408796
1108	15	0.6727517813010948
1108	16	0.9149178640028168
1108	17	1.085484918984612
1108	18	0.7605908850847953
1108	19	0.7712552912884476
1108	20	0.6466536886833225
1108	21	0.7053020489491075
1108	22	1.0866372034299374
1108	23	2.6514306018407154
1108	24	1.3917721815266972
1108	25	1.64929543168477
1109	1	0.3404084162968317
1109	2	0.5764290771401654
1109	3	0.45623287275767765
1109	4	0.3922119488573017
1109	5	0.35992384242372566
1109	6	0.21256303261601772
1109	7	0.2548417293017309
1109	8	0.05783184626189808
1109	9	0.1758863638842385
1109	10	0.7734764893132464
1109	11	0.3828011143742529
1109	12	0.408840696746978
1109	13	0.18159053099095246
1109	14	-0.9507709978137271
1109	15	-0.08413901454970585
1109	16	-0.40246349656268166
1109	17	0.6834219012512932
1109	18	1.5343997521864452
1109	19	0.7617507824233485
1109	20	0.6040191535563437
1109	21	0.4534116832775865
1109	22	0.4294277398688777
1109	23	-4.395568667392993
1109	24	-1.686894265124316
1109	25	-0.31001343834856243
1110	1	0.5089434276607747
1110	2	0.8286242370150738
1110	3	0.644192240326479
1110	4	1.1188656951742857
1110	5	0.8447966059697976
1110	6	0.39914723881501857
1110	7	0.3421858153926891
1110	8	0.2916607236817512
1110	9	0.32736473733202587
1110	10	0.7596569365071488
1110	11	0.38331140743749614
1110	12	0.5117511435440818
1110	13	0.32625723026223596
1110	14	1.0818804914022948
1110	15	0.566114609407677
1110	16	1.023596030553442
1110	17	1.2722965204923895
1110	18	1.83330898922911
1110	19	1.1469987451728727
1110	20	0.7338431762713071
1110	21	0.5493801143155821
1110	22	0.8249579232883235
1110	23	2.1816109668385337
1110	24	1.3542106254764192
1110	25	1.5151584741246467
1111	1	0.32552886771074796
1111	2	0.5932985121137833
1111	3	0.35932760698814903
1111	4	0.9483536650895985
1111	5	0.6148985010669534
1111	6	0.09184952603859386
1111	7	0.016868927778794784
1111	8	-0.24092214732635142
1111	9	-0.061453792266121354
1111	10	0.19622186755103144
1111	11	-0.0739798511402654
1111	12	0.06619546863349512
1111	13	-0.08236395431340071
1111	14	-0.7083907745052755
1111	15	-0.1451191264630438
1111	16	0.2533088593339343
1111	17	0.7536125180587447
1111	18	1.3885600891509355
1111	19	0.5232907132611473
1111	20	0.28771790704546873
1111	21	0.1464916573113797
1111	22	0.4675893948636656
1111	23	0.47728119249176615
1111	24	0.3602188976829268
1111	25	-0.10621724508973074
1112	1	0.5560667818141347
1112	2	0.9382915676132981
1112	3	0.6674311979539311
1112	4	1.2993472800943806
1112	5	0.9816864835393702
1112	6	0.48679748039228343
1112	7	0.46809827024353723
1112	8	0.40894748077300846
1112	9	0.5094218065793612
1112	10	0.8559617424334576
1112	11	0.44479002631552333
1112	12	0.5514918674459821
1112	13	0.42742380130832913
1112	14	0.8079514418708342
1112	15	0.6285331966046215
1112	16	0.927256043471246
1112	17	1.1637919722003858
1112	18	1.2009944660871241
1112	19	0.7630956575303003
1112	20	0.6867919885915654
1112	21	0.6534166558095009
1112	22	0.7242207427602807
1112	23	2.264822068207174
1112	24	1.313767631578095
1112	25	1.3207036557496181
1113	1	0.38452076419130066
1113	2	0.7455121274698939
1113	3	0.4591279895003204
1113	4	0.52138215634929
1113	5	0.7337921466649391
1113	6	0.12766757487446512
1113	7	0.20344086708733045
1113	8	0.07198948672937888
1113	9	0.29581046238524067
1113	10	0.5506172809255787
1113	11	0.35159420316308143
1113	12	0.26918612234555894
1113	13	0.06419603259000439
1113	14	0.9575375216256816
1113	15	0.39710199655255707
1113	16	0.6786936818917653
1113	17	1.0597459149974213
1113	18	1.8728122672963394
1113	19	0.8907604999925738
1113	20	0.5142511902690572
1113	21	0.5443448011547929
1113	22	0.7555721668534972
1113	23	0.8386451749134523
1113	24	0.6834465223492359
1113	25	1.140678254099214
1114	1	-0.36520654679441833
1114	2	-3.056030819976275
1114	3	-1.1322735032036384
1114	4	0.20961186785719832
1114	5	0.30071947826336753
1114	6	0.026446271635691687
1114	7	0.045336635469148234
1114	8	0.03815844226603815
1114	9	0.14793271294994526
1114	10	0.2433465758849067
1114	11	0.11462229657302458
1114	12	0.17156431409532047
1114	13	0.13662137592049817
1114	14	0.8398870273500094
1114	15	0.21596134055296545
1114	16	0.5771339866163743
1114	17	0.9303444566479797
1114	18	1.0736891664572354
1114	19	0.547106209542271
1114	20	0.31093168463062426
1114	21	0.11008647233048663
1114	22	0.43462707396746225
1114	23	1.9414540864763268
1114	24	0.9378443305307655
1114	25	0.878968086019718
1115	1	0.21703886208035783
1115	2	0.5222421944565738
1115	3	0.3331876255494919
1115	4	0.42021808120800586
1115	5	0.542159739029195
1115	6	0.12127842043351222
1115	7	-0.017765923463592515
1115	8	-0.018538383850867035
1115	9	-0.03834767752672974
1115	10	0.3164252760882107
1115	11	0.2667389187146014
1115	12	0.1433597674951912
1115	13	0.12023446125746062
1115	14	0.8701195659524217
1115	15	0.19080886310954526
1115	16	0.6721757393928911
1115	17	1.1963571084953557
1115	18	1.680943396341128
1115	19	0.9136640837630807
1115	20	0.5153211867896701
1115	21	0.4840846577228959
1115	22	0.7562498876173436
1115	23	2.4717685344491755
1115	24	1.286256128840492
1115	25	0.936616320977798
1116	1	0.11382510614873453
1116	2	0.049781434432034644
1116	3	0.18995957935466024
1116	4	0.9769232435655866
1116	5	0.31004159583144286
1116	6	0.11206916127493116
1116	7	-2.6986493564606917E-4
1116	8	0.026235767858914016
1116	9	0.06143408530149941
1116	10	0.564513370901004
1116	11	0.0011475814100236903
1116	12	0.09659837605847343
1116	13	-0.08090720650945116
1116	14	0.6026508841515366
1116	15	0.12407993542294093
1116	16	0.48901223237590585
1116	17	0.7870946491721185
1116	18	1.3356098662577978
1116	19	0.48764784412158235
1116	20	0.22101205111760436
1116	21	0.10210708722296857
1116	22	0.448539164483428
1116	23	1.7234703572343761
1116	24	0.7484321709035697
1116	25	0.9954817206780078
1201	1	1.0593901700430628
1201	2	1.4754833693709237
1201	3	1.1529977109010865
1201	4	1.7243674147335142
1201	5	1.5138868452962484
1201	6	0.9917510213481933
1201	7	0.9410388460226145
1201	8	0.9304401895893406
1201	9	1.0642416021763594
1201	10	1.3118147869735992
1201	11	0.9988330766676796
1201	12	1.1868057777040022
1201	13	0.9933278947508271
1201	14	1.967301000439548
1201	15	1.3164976450784636
1201	16	1.668395320856206
1201	17	2.0591349326318067
1201	18	2.6957663730929937
1201	19	1.7092511142273707
1201	20	1.5036580017651247
1201	21	1.3957961084534745
1201	22	1.6704705149956567
1201	23	3.314732764724951
1201	24	2.10311918509327
1201	25	2.1805949607952044
1202	1	1.1770693779840187
1202	2	1.5065667266098615
1202	3	1.2425545512776281
1202	4	1.7968537121672625
1202	5	1.5162314924890836
1202	6	1.21274570968189
1202	7	1.014748851257508
1202	8	1.0041133482628268
1202	9	1.3028986562227587
1202	10	1.6647035506883938
1202	11	1.3492559345057344
1202	12	1.3264035981562046
1202	13	1.2766490106576498
1202	14	2.0737529941265933
1202	15	1.5899259282042308
1202	16	1.9823816190268886
1202	17	2.3544885276436176
1202	18	3.026579892913695
1202	19	2.20451820691175
1202	20	1.8654563980151906
1202	21	1.8418763913493095
1202	22	2.089498052507807
1202	23	3.6261900489082777
1202	24	2.4133531634379466
1202	25	2.6336957865199437
1203	1	1.2513417355698664
1203	2	1.5559458434295053
1203	3	1.2640679256310925
1203	4	1.8996980686426426
1203	5	1.6782122830497812
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2211	14	-0.07538853033683779
2211	15	-0.19951313728164877
2211	16	-0.5112420612665503
2211	17	0.1160648317545423
2211	18	-5.474766094270247
2211	19	-1.768244831236359
2211	20	-0.9096667630817947
2211	21	-0.7511067821452144
2211	22	-0.3646469562968164
2211	23	-4.649965706078376
2211	24	-1.8253367933680664
2211	25	-1.2337419345827243
2212	1	0.30034560855712655
2212	2	0.4835116636838528
2212	3	0.2822094768862762
2212	4	0.7066669133821506
2212	5	0.634566620202996
2212	6	0.18353569700121142
2212	7	-0.017523691706038846
2212	8	-0.06657161931543243
2212	9	-0.16412856628236483
2212	10	-3.972024235252647
2212	11	-1.1620107458539621
2212	12	-0.4239360825951479
2212	13	-0.25187460050474897
2212	14	0.5341501275804674
2212	15	0.07924480422745006
2212	16	0.326083701377037
2212	17	0.8226826636070683
2212	18	1.4152642165157943
2212	19	0.6385282504202792
2212	20	0.2595810480368712
2212	21	0.06537378956952011
2212	22	0.3430312448843793
2212	23	1.5983716126390064
2212	24	0.8085081259140452
2212	25	0.7312896697591853
2213	1	-1.518194456066336
2213	2	-3.9318410582249683
2213	3	-1.8730467649538376
2213	4	-3.2790120172554182
2213	5	-1.379118307975041
2213	6	-1.0222909460572147
2213	7	-0.9318882113203522
2213	8	-0.8146879397070563
2213	9	-0.8642613367019933
2213	10	-0.7808360984273293
2213	11	-0.9640092961295679
2213	12	-0.8613371284200113
2213	13	-1.2183523451843428
2213	14	-6.3303950084282725
2213	15	-2.924441381023634
2213	16	-2.1883035630787546
2213	17	-0.9555652715265808
2213	18	-0.13048212365600875
2213	19	-0.8631547411047933
2213	20	-1.0459261878530555
2213	21	-1.1612373062490562
2213	22	-1.0414764070725298
2213	23	-5.764674887196051
2213	24	-3.169284082790959
2213	25	-2.218278886868614
2214	1	-0.024518648043134306
2214	2	0.05632965768065645
2214	3	-0.012502586221870615
2214	4	0.2695275372395969
2214	5	0.01827817835042822
2214	6	-0.25219560260903506
2214	7	-0.4011653980811829
2214	8	-0.3635307688723586
2214	9	-0.1719837360683627
2214	10	0.4178401373916856
2214	11	-0.1828763715489643
2214	12	-0.08852612367024193
2214	13	-0.22917209852693077
2214	14	0.3152303919987176
2214	15	-0.12299511678146047
2214	16	0.28282078088344775
2214	17	0.6385891063965516
2214	18	1.0571693312202868
2214	19	0.16927970452011465
2214	20	0.047139275685601945
2214	21	-0.11425376078064886
2214	22	0.3443393883166621
2214	23	1.76149397784706
2214	24	0.8182234934616801
2214	25	0.6251394240048782
2215	1	-2.4513416006445787
2215	2	-4.436948036905893
2215	3	-2.523496179077757
2215	4	-3.826247214421816
2215	5	-4.806811983335841
2215	6	-2.3167035847078736
2215	7	-1.695786489016296
2215	8	-2.064810877424133
2215	9	-1.6643116878095014
2215	10	-3.1803613563025337
2215	11	-1.9331325465810352
2215	12	-1.5693651682374536
2215	13	-1.5446112970972905
2215	14	-0.7863714366182677
2215	15	-1.4486512911321014
2215	16	-2.1957548518667522
2215	17	-6.561676310527783
2215	18	-2.9443779404197947
2215	19	-2.152852625748487
2215	20	-1.8625708440586592
2215	21	-1.7752341083439447
2215	22	-1.521706410583171
2215	23	-6.27630344498527
2215	24	-3.5305819174319986
2215	25	-3.460877252286984
2216	1	-1.1464147831446851
2216	2	-0.6545719103299632
2216	3	-0.958519050106883
2216	4	-3.662213485445509
2216	5	-1.5598531991191678
2216	6	-1.2953100256625945
2216	7	-1.2265431502135122
2216	8	-1.0656542616015017
2216	9	-1.12327804191343
2216	10	-0.9323926280212262
2216	11	-1.4994647221167767
2216	12	-2.1063917906585736
2216	13	-1.4479592307161866
2216	14	-6.949738526621321
2216	15	-3.347392465854618
2216	16	-1.9199398667492957
2216	17	-1.0426956730148191
2216	18	-0.29190774145861553
2216	19	-0.8815221757252374
2216	20	-1.285225145617531
2216	21	-1.56318519805302
2216	22	-0.999322279546476
2216	23	0.03382806942355643
2216	24	-0.6644487350277544
2216	25	-0.5716617453617658
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90792.0
3	1.0
4	757.0
5	4622.0
6	5957.0
7	5304.0
8	5048.0
9	4532.0
10	4205.0
11	4372.0
12	4171.0
13	4066.0
14	4028.0
15	4149.0
16	4225.0
17	4404.0
18	4953.0
19	6009.0
20	7370.0
21	9396.0
22	11538.0
23	14108.0
24	16764.0
25	19453.0
26	22171.0
27	25127.0
28	28049.0
29	34109.0
30	39745.0
31	45477.0
32	58554.0
33	77123.0
34	99521.0
35	131085.0
36	183575.0
37	255862.0
38	625733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38752058711501	17.63238789075283	11.701421114467042	25.27867040766511
2	13.547271762378784	20.15631281861602	40.73573163083158	25.560683788173616
3	20.067663321377193	25.312213915575217	28.197713682955744	26.422409080091846
4	10.99195236270377	16.490072436775264	37.9031121282691	34.614863072251865
5	12.150033633218364	38.48513840538346	35.87702301798506	13.487804943413115
6	32.9317002194575	34.51624226606608	18.460367691784143	14.09168982269228
7	28.349605512640636	31.250783776338903	21.639515403817647	18.76009530720281
8	26.781176006525627	35.204092023362776	20.601230308155856	17.41350166195574
9	27.921658544062783	14.905983070896317	19.29728087058449	37.875077514456414
10	14.85266276883363	29.44825723125213	34.112149532710276	21.586930467203956
11	33.66826764876432	23.42489407472274	22.44302865368517	20.46380962282777
12	24.236977395634543	25.76272785102845	30.401663051918927	19.598631701418086
13	29.30509855199837	21.166707752328485	24.423800387991555	25.104393307681587
14	21.280466988546625	22.555231674788693	27.654772757357925	28.509528579306753
15	26.55495763749659	27.755438472945503	22.379749603278597	23.309854286279307
16	21.576580246013748	27.591440539532414	29.03888286264304	21.793096351810803
17	23.499943993264186	27.723441418294207	27.795097883159762	20.981516705281837
18	22.928630991809058	25.911753924513015	29.926461603760846	21.233153479917082
19	23.69694309241152	27.49003193960044	28.17861735136616	20.634407616621885
20	24.164203133742504	26.792823133699628	27.382248842907703	21.660724889650158
21	22.456900848637645	26.772175575914854	28.406904439563647	22.364019135883854
22	23.058562713834014	27.475543258322478	25.428364835464055	24.037529192379456
23	20.45529581257343	27.52329325706837	28.934073543662265	23.08733738669594
24	22.620074368572016	29.072301110704323	26.419228837467095	21.888395683256565
25	22.707702334545885	26.204670592504176	26.221982581887588	24.86564449106235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	246.0
2	396.0
3	546.0
4	546.0
5	546.0
6	1057.5
7	1569.0
8	1569.0
9	1569.0
10	2326.0
11	3083.0
12	3083.0
13	3083.0
14	5116.0
15	7149.0
16	7149.0
17	7149.0
18	12490.5
19	17832.0
20	17832.0
21	17832.0
22	28032.5
23	38233.0
24	38233.0
25	38233.0
26	54414.0
27	70595.0
28	70595.0
29	70595.0
30	90462.0
31	110329.0
32	110329.0
33	110329.0
34	129460.0
35	148591.0
36	148591.0
37	148591.0
38	167318.5
39	186046.0
40	186046.0
41	186046.0
42	207154.0
43	228262.0
44	228262.0
45	228262.0
46	276730.5
47	325199.0
48	325199.0
49	325199.0
50	298572.5
51	271946.0
52	271946.0
53	271946.0
54	227889.0
55	183832.0
56	183832.0
57	183832.0
58	153452.5
59	123073.0
60	123073.0
61	123073.0
62	99597.0
63	76121.0
64	76121.0
65	76121.0
66	58950.5
67	41780.0
68	41780.0
69	41780.0
70	30549.5
71	19319.0
72	19319.0
73	19319.0
74	13585.5
75	7852.0
76	7852.0
77	7852.0
78	5490.5
79	3129.0
80	3129.0
81	3129.0
82	2127.0
83	1125.0
84	1125.0
85	1125.0
86	751.0
87	377.0
88	377.0
89	377.0
90	237.5
91	98.0
92	98.0
93	98.0
94	56.5
95	15.0
96	15.0
97	15.0
98	11.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12457437089942697
2	0.04634702401204487
3	0.03466650235351795
4	0.03161242100243523
5	0.03429143973145517
6	0.02089634608635549
7	0.02084276571177509
8	0.02577216017317177
9	0.03075513500914885
10	0.03766700333002028
11	0.022343016200026253
12	0.020789185337194694
13	0.01794942548443356
14	0.023682525564536223
15	0.02336104331705383
16	0.023736105939116618
17	0.027218830286842535
18	0.020949926460935887
19	0.034612921978937554
20	0.02523635642736778
21	0.02898698264799569
22	0.03825638745040466
23	0.031023036882050842
24	0.041899852921871775
25	0.03193390324991762
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1866355.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.66310515809771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05021766859447	58.39812039497802
2	10.276429680541204	14.112231436095943
3	2.48918803362628	5.1274613913347915
4	0.907941315344022	2.4936828005139255
5	0.41634827202482244	1.4293882592216325
6	0.21962634207909576	0.9048135972999173
7	0.14091675806789775	0.6773047524427993
8	0.09510399470223325	0.5224108471355687
9	0.06556641361073264	0.4051794197333757
>10	0.2994880836464485	3.563545066820083
>50	0.019485369675838828	0.919322134408245
>100	0.014746841220300953	2.0543944937515017
>500	0.0017533385655624447	0.8636864392832819
>1k	0.002550310640818101	3.524900101374474
>5k	3.9848603762782824E-4	1.7773345917431231
>10k+	2.39091622576697E-4	3.226224273863344
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	26357	1.4122179328155684	No Hit
CCCATGTACTCTGCGTTGATACCAC	20899	1.1197762483557523	No Hit
GAGTACATGGGAAGCAGTGGTATCA	11700	0.6268903825906647	No Hit
GTATCAACGCAGAGTACATGGGAAG	7252	0.3885648764570513	No Hit
GCGTTGATACCACTGCTTCCCATGT	7247	0.3882969745841493	No Hit
CATGTACTCTGCGTTGATACCACTG	6682	0.3580240629462241	No Hit
TATCAACGCAGAGTACATGGGAAGC	5874	0.3147311202852619	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5424	0.2906199517240825	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4804	0.2574001194842353	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4578	0.2452909548290652	No Hit
GGTATCAACGCAGAGTACATGGGAA	3667	0.196479233586322	No Hit
CAGTGGTATCAACGCAGAGTACATG	2571	0.13775514304620504	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2523	0.13518328506634592	No Hit
CCACTGCTTCCCATGTACTCTGCGT	2432	0.1303074709795296	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2291	0.12275263816369342	No Hit
GTGGTATCAACGCAGAGTACATGGG	2257	0.12093090542795984	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2218	0.1188412708193243	No Hit
ATACCACTGCTTCCCATGTACTCTG	2205	0.11814472594977911	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2174	0.11648373433778675	No Hit
GTACTCTGCGTTGATACCACTGCTT	2099	0.11246520624425685	No Hit
CCCATGTACTCTGCGTTTATACCAC	1972	0.10566049867254622	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1939	0.10389234631139306	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1916	0.10265999769604389	No Hit
GTACATGGGAAGCAGTGTTATCAAC	1888	0.10115974720779272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.358037458039869E-5
7	0.0	5.358037458039869E-5	0.0	0.0	5.358037458039869E-5
8	5.358037458039869E-5	5.358037458039869E-5	0.0	0.0	5.358037458039869E-5
9	5.358037458039869E-5	5.358037458039869E-5	0.0	0.0	5.358037458039869E-5
10	5.358037458039869E-5	5.358037458039869E-5	0.0	0.0	5.358037458039869E-5
11	5.358037458039869E-5	5.358037458039869E-5	0.0	5.358037458039869E-5	5.358037458039869E-5
12	5.358037458039869E-5	5.358037458039869E-5	0.0	1.6074112374119608E-4	2.1432149832159477E-4
13	5.358037458039869E-5	5.358037458039869E-5	0.0	1.6074112374119608E-4	3.2148224748239216E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGA	35	0.0021713185	16.284704	18
TATCGAC	120	0.0	15.832351	19
GTATCGA	105	1.373337E-9	13.570586	18
GCGTTTA	335	0.0	13.325745	13
GTCTAGA	190	0.0	12.517677	1
GTAGGAC	870	0.0	12.33997	3
CGTTTAT	365	0.0	12.230478	14
GCACCGT	110	3.8186045E-8	12.090158	6
TGTAGGA	930	0.0	12.054307	2
TAGGACC	345	0.0	11.842384	4
TGCGTTT	395	0.0	11.7825	12
GTCCTAA	450	0.0	11.627531	1
GGCCTAT	90	7.346209E-6	11.627531	1
TGTACCG	90	7.4522704E-6	11.612569	5
GGTATCG	115	7.089875E-8	11.563569	17
GGACGTG	740	0.0	11.424966	6
AGGACGT	750	0.0	11.274749	5
AGCACCG	110	4.9615664E-7	11.228682	5
TAGGACG	820	0.0	11.123646	4
CGTGAAA	510	0.0	10.989219	9
>>END_MODULE