FastQCFastQC Report
Thu 26 May 2016
SRR1513097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866355
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC299931.607036174789898No Hit
CCCATGTACTCTGCGTTGATACCAC284341.5235043708190563No Hit
GAGTACATGGGAAGCAGTGGTATCA120330.6447326473259374No Hit
CATGTACTCTGCGTTGATACCACTG85060.4557546661808713No Hit
GCGTTGATACCACTGCTTCCCATGT77050.41283678614197195No Hit
GTATCAACGCAGAGTACATGGGAAG76800.41149727677746195No Hit
ACGCAGAGTACATGGGAAGCAGTGG69570.3727586659558337No Hit
TATCAACGCAGAGTACATGGGAAGC67460.36145320691936955No Hit
GCTTCCCATGTACTCTGCGTTGATA58980.3160170492751915No Hit
ACTCTGCGTTGATACCACTGCTTCC58030.3109269136900536No Hit
GGTATCAACGCAGAGTACATGGGAA44380.2377897023878094No Hit
GTATCAACGCAGAGTACTTTTTTTT42040.2252518947359961No Hit
CCACTGCTTCCCATGTACTCTGCGT32150.17226090427598179No Hit
CAGTGGTATCAACGCAGAGTACATG32140.1722073239014014No Hit
TATCAACGCAGAGTACTTTTTTTTT29130.1560796311527014No Hit
ATACCACTGCTTCCCATGTACTCTG28900.15484728253735222No Hit
CATGGGAAGCAGTGGTATCAACGCA26980.14455985061791568No Hit
GGGAAGCAGTGGTATCAACGCAGAG25910.13882675053781302No Hit
ACATGGGAAGCAGTGGTATCAACGC25900.13877317016323262No Hit
GCAGTGGTATCAACGCAGAGTACAT25830.13839810754116982No Hit
GGTATCAACGCAGAGTACTTTTTTT25620.13727291967498145No Hit
TACCACTGCTTCCCATGTACTCTGC25140.1347010616951223No Hit
GTGGTATCAACGCAGAGTACATGGG25140.1347010616951223No Hit
CTGCTTCCCATGTACTCTGCGTTGA24390.1306825336015924No Hit
GTACTCTGCGTTGATACCACTGCTT23010.12328844190949738No Hit
AAGCAGTGGTATCAACGCAGAGTAC22080.11830546707352031No Hit
CCATGTACTCTGCGTTGATACCACT20780.11134001837806848No Hit
CTTCCCATGTACTCTGCGTTGATAC20480.10973260714065651No Hit
GATACCACTGCTTCCCATGTACTCT19550.10474963230467943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3600.012.9612444
ACGAAAT550.003087885412.08092412
ACCGTGC802.9129144E-511.8566088
ACTGCGC600.00594458711.0661688
TATAAGG1657.6397555E-1110.9730322
TGGCGAA700.001505321910.84526918
TTAGGAC5100.010.8295623
GTAGGAC9950.010.814553
TAGACTC1158.5530337E-710.7646175
CTAACAC2050.010.6838323
GCGGTAT803.8003316E-410.67867515
AGGACGT9000.010.5806065
GTCCTAC9200.010.5669871
TAGGACT2650.010.4208994
GACGTGG5150.010.3143767
TGTAGGA11000.010.3088742
GGACGTG9250.010.2946456
GAACCGC750.002596424710.1573836
GCTATAC1803.765308E-1010.0515773
GGCGAGG3400.010.04243819
Produced by FastQC (version 0.11.5)