FastQCFastQC Report
Thu 26 May 2016
SRR1513096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985959
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC257311.2956460833280041No Hit
CCCATGTACTCTGCGTTGATACCAC204901.0317433542182894No Hit
GAGTACATGGGAAGCAGTGGTATCA111810.5630025594687503No Hit
GTATCAACGCAGAGTACATGGGAAG72570.3654153988073268No Hit
GCGTTGATACCACTGCTTCCCATGT71140.35821484733572045No Hit
CATGTACTCTGCGTTGATACCACTG67340.3390805147538293No Hit
TATCAACGCAGAGTACATGGGAAGC56810.2860582720992729No Hit
ACGCAGAGTACATGGGAAGCAGTGG54300.2734195418938659No Hit
GCTTCCCATGTACTCTGCGTTGATA48820.24582582017050703No Hit
ACTCTGCGTTGATACCACTGCTTCC46330.2332877969786889No Hit
GGTATCAACGCAGAGTACATGGGAA37550.1890774180131614No Hit
CAGTGGTATCAACGCAGAGTACATG27610.13902603225947766No Hit
CCACTGCTTCCCATGTACTCTGCGT24750.12462492931626483No Hit
GTCCTACAGTGGACATTTCTAAATT24110.12140230488142001No Hit
GTGGTATCAACGCAGAGTACATGGG22620.11389963236904689No Hit
GTATCAACGCAGAGTACTTTTTTTT22460.11309397626033568No Hit
CTGCTTCCCATGTACTCTGCGTTGA21990.11072736144099651No Hit
GCAGTGGTATCAACGCAGAGTACAT21780.10966993779831305No Hit
GTACTCTGCGTTGATACCACTGCTT21530.10841110012845179No Hit
ATACCACTGCTTCCCATGTACTCTG21500.10826003960806845No Hit
GGGAAGCAGTGGTATCAACGCAGAG21160.10654802037705713No Hit
CTGTAGGACGTGGAATATGGCAAGA20290.10216726528593995No Hit
ACATGGGAAGCAGTGGTATCAACGC20120.10131125567043428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA902.2901077E-914.77720919
GCGTTTA3900.014.36979513
TCCAACG1600.014.24945218
TATCGAC801.2896271E-714.24945219
TAGGACC5900.014.1710874
TGCGTTT4300.013.91666812
TTACGAC604.0907649E-412.6674573
CGTTTAT4300.012.59127114
GTAGGAC13100.012.1114413
TTATCGA550.003069844612.09044518
GGTATCG550.003069844612.09044517
GTGTAGG3450.011.85415651
GCCTTAG1850.011.8243531
ACGTTAA657.948364E-411.7057131
AGGACCT10200.011.1768865
GGACCGA600.00588051911.0823496
CGAAATC600.005882723711.08179113
TGTAGGA15600.011.0239552
GTATTAG2850.011.0126121
CGCAGAT1658.0035534E-1110.9378718
Produced by FastQC (version 0.11.5)