##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513096_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985959 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.402030958343047 31.0 30.0 34.0 25.0 34.0 2 29.149996047249715 31.0 30.0 34.0 18.0 34.0 3 29.497175420036367 31.0 30.0 34.0 24.0 34.0 4 32.33777031650704 35.0 33.0 37.0 19.0 37.0 5 32.57744898056808 35.0 33.0 37.0 24.0 37.0 6 33.055105367230645 37.0 35.0 37.0 27.0 37.0 7 33.0533137894589 37.0 35.0 37.0 27.0 37.0 8 33.03669209686605 37.0 35.0 37.0 26.0 37.0 9 34.41160416705481 39.0 35.0 39.0 27.0 39.0 10 33.93037217787477 39.0 34.0 39.0 22.0 39.0 11 34.294195902332326 39.0 35.0 39.0 27.0 39.0 12 34.13593533401244 39.0 35.0 39.0 25.0 39.0 13 34.21748384533618 39.0 35.0 39.0 26.0 39.0 14 34.546956407458566 40.0 34.0 41.0 17.0 41.0 15 35.083556609174714 40.0 34.0 41.0 25.0 41.0 16 34.66017928869629 39.0 34.0 41.0 18.0 41.0 17 34.220680789482564 39.0 33.0 41.0 17.0 41.0 18 33.63605643419628 39.0 32.0 41.0 11.0 41.0 19 34.51695276690002 39.0 34.0 41.0 18.0 41.0 20 34.790268077034824 40.0 34.0 41.0 19.0 41.0 21 34.81222019185693 40.0 35.0 41.0 18.0 41.0 22 34.414904335890114 40.0 34.0 41.0 13.0 41.0 23 32.788976006050476 39.0 32.0 41.0 8.0 41.0 24 33.88041495317879 39.0 33.0 41.0 2.0 41.0 25 33.61005841510323 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.925437208099396 1101 2 1.2025810431853294 1101 3 0.9973622691530615 1101 4 0.9678960540229369 1101 5 1.1928060814339574 1101 6 0.9143721816973667 1101 7 0.9945648003330518 1101 8 0.904595864488499 1101 9 1.0036155622800536 1101 10 1.4262068400690495 1101 11 0.9946351699064024 1101 12 1.0816806379386108 1101 13 1.022242086183276 1101 14 0.22033693290913448 1101 15 0.780906890394526 1101 16 1.4557126580213335 1101 17 2.0490322276009465 1101 18 -4.148906385093788 1101 19 -0.3765927293253526 1101 20 0.49348234191632656 1101 21 0.8758093589870981 1101 22 1.4265449440384614 1101 23 3.3354795717581496 1101 24 2.2904185405261828 1101 25 2.451455621922605 1102 1 0.9702141639388948 1102 2 1.211037396093328 1102 3 1.0436975331977187 1102 4 1.5882909549926367 1102 5 1.353548646026013 1102 6 0.9758553042433036 1102 7 0.9662714280239371 1102 8 0.8464664037860672 1102 9 0.9360982889216984 1102 10 1.2545624452783528 1102 11 1.0421215392290648 1102 12 1.1439951142964304 1102 13 1.0136478898870607 1102 14 1.84442513279199 1102 15 1.3303431787285405 1102 16 1.6350247607612047 1102 17 1.3092197578812943 1102 18 1.705914472734996 1102 19 1.4831414543887007 1102 20 1.4122614020142308 1102 21 1.400502081217759 1102 22 1.8523449780247674 1102 23 3.3007410041724086 1102 24 2.1739973706742006 1102 25 2.4500295077864678 1103 1 0.685374874210865 1103 2 0.9926469821547101 1103 3 0.7038440684829226 1103 4 1.175522183104789 1103 5 1.068234439861527 1103 6 0.7158236008521826 1103 7 0.6384637564921007 1103 8 0.5333797822908437 1103 9 0.7503200875861324 1103 10 0.9890034940098573 1103 11 0.793597496131305 1103 12 0.9263227395692368 1103 13 0.5779755024712543 1103 14 1.4893369560360625 1103 15 0.9422387931834066 1103 16 1.1527688714348656 1103 17 1.5533234074872198 1103 18 1.7311382907006134 1103 19 1.1378119493893877 1103 20 1.0470406927573492 1103 21 0.8707715419157083 1103 22 1.324817498127146 1103 23 2.771261064860205 1103 24 1.8355559666617012 1103 25 1.8823310307818488 1104 1 0.553547497272227 1104 2 0.9639108292502314 1104 3 0.8245587067281441 1104 4 1.2606060888066253 1104 5 1.0650977573051534 1104 6 0.7071350569563251 1104 7 0.6540135986895805 1104 8 0.7263802606961534 1104 9 0.8650834884445899 1104 10 1.1619687186855927 1104 11 0.7807970931043258 1104 12 0.9165491572929838 1104 13 0.7099776342577826 1104 14 1.6329741988097268 1104 15 1.0718827363028183 1104 16 1.440824077047857 1104 17 1.8919663570641063 1104 18 2.521294672914621 1104 19 1.67264648994108 1104 20 1.082250344033497 1104 21 1.2314885888837992 1104 22 1.4324791736321032 1104 23 2.8325068509865403 1104 24 1.7539680483882023 1104 25 1.8592756348307802 1105 1 0.6870129746425597 1105 2 1.0086272327249333 1105 3 0.6986124388319581 1105 4 1.0000478445990808 1105 5 0.8904610579743917 1105 6 0.6970959928842433 1105 7 0.5345950237271282 1105 8 0.6330987423092509 1105 9 0.6925213283411153 1105 10 1.1445538665727284 1105 11 0.7204247405249262 1105 12 0.7296386371072643 1105 13 0.7512567222239852 1105 14 1.5072849988362975 1105 15 0.8774524613143058 1105 16 1.4151674550076123 1105 17 1.807846032698265 1105 18 1.7278997370763491 1105 19 1.1918171366888544 1105 20 1.1377148478831316 1105 21 0.9692572763966325 1105 22 1.4274679305914546 1105 23 2.83626503786914 1105 24 1.905403958693853 1105 25 1.9068672290478048 1106 1 -0.5234014274589569 1106 2 -0.06550830283140385 1106 3 -0.31046913346894556 1106 4 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1107 16 -5.717222992006821 1107 17 -0.8744219080041802 1107 18 0.7846527635193468 1107 19 0.6232423498095159 1107 20 0.47931337044708755 1107 21 0.5334977004607993 1107 22 0.4869664552781643 1107 23 2.432511669332243 1107 24 1.3970158311336789 1107 25 1.108538891650845 1108 1 0.4857008896026116 1108 2 0.6968108336759293 1108 3 0.43046918923857547 1108 4 1.022916104335522 1108 5 0.8359526172253027 1108 6 0.4876236562378864 1108 7 0.3197888495990995 1108 8 0.42352700528108045 1108 9 0.457283503182488 1108 10 0.9256076340583377 1108 11 0.5127598016707822 1108 12 0.6433795704768528 1108 13 0.39189510655848636 1108 14 1.0567936978314734 1108 15 0.7503307422983951 1108 16 0.9912300239747509 1108 17 1.168909798692873 1108 18 0.9852970257847602 1108 19 0.8546114113567995 1108 20 0.746687713852765 1108 21 0.7816413295868827 1108 22 1.221354742142296 1108 23 2.7377391195444147 1108 24 1.5290052475205016 1108 25 1.7722034248522647 1109 1 0.30184461047365474 1109 2 0.6614045238386126 1109 3 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0.8705639637150071 2212 20 0.3895936655648171 2212 21 0.255378507594763 2212 22 0.551115413618362 2212 23 1.9123481470295545 2212 24 1.0809091273123386 2212 25 0.968747277152282 2213 1 -1.4590707992577414 2213 2 -3.832662359681578 2213 3 -1.6254809207217527 2213 4 -3.339380892977246 2213 5 -1.18692657530298 2213 6 -0.9943250661647625 2213 7 -0.9728501672408072 2213 8 -0.8545022285534074 2213 9 -0.7728127469997403 2213 10 -0.705573744131776 2213 11 -0.8447009057354933 2213 12 -0.6726119801033477 2213 13 -1.0122942423336951 2213 14 -6.145569906385788 2213 15 -2.6588424408230296 2213 16 -1.931348496590985 2213 17 -0.6928690874767298 2213 18 0.022006404826846904 2213 19 -0.5520642713329522 2213 20 -0.7635916898076189 2213 21 -0.9571297409728032 2213 22 -0.8520896578423987 2213 23 -5.535635178792891 2213 24 -2.7925471834454 2213 25 -1.880065935041113 2214 1 0.0702294684996545 2214 2 0.07458427741880058 2214 3 0.09437290865315617 2214 4 0.3727783632886954 2214 5 0.12093166487530738 2214 6 -0.06170443291320993 2214 7 -0.12280821246699247 2214 8 -0.1505050828298451 2214 9 -0.04610923351675922 2214 10 0.3652304646707165 2214 11 -0.14638189689167547 2214 12 -0.09153189331635048 2214 13 -0.11501903350126952 2214 14 0.37918901057272336 2214 15 -0.001177366410033187 2214 16 0.4472057100389293 2214 17 0.8568376153415258 2214 18 1.299430584487908 2214 19 0.4201162857916785 2214 20 0.1676389803390279 2214 21 0.11582521727036976 2214 22 0.43373870512963464 2214 23 1.989914940505436 2214 24 0.8864493587860167 2214 25 0.9798416196183766 2215 1 -2.19593182435824 2215 2 -3.9293316137059833 2215 3 -2.0565086586358667 2215 4 -3.68737734889158 2215 5 -4.4309456960956695 2215 6 -2.102311835912758 2215 7 -1.4752085422550039 2215 8 -1.7873704319291086 2215 9 -1.3972255466303167 2215 10 -2.828316994084613 2215 11 -1.601821035785413 2215 12 -1.410088151852598 2215 13 -1.306683680658061 2215 14 -0.6691499960579108 2215 15 -1.2766107368367798 2215 16 -1.9444806667709216 2215 17 -6.433547231749927 2215 18 -2.593946579723152 2215 19 -1.723635842434767 2215 20 -1.621681468931115 2215 21 -1.529738914485641 2215 22 -1.2342689889525502 2215 23 -5.739174571549103 2215 24 -3.219497908828508 2215 25 -2.9565290201414065 2216 1 -1.2578533517271673 2216 2 -0.7983561268629131 2216 3 -1.1537029767235758 2216 4 -3.3726659543757016 2216 5 -1.6225610196560254 2216 6 -1.2891599053656506 2216 7 -1.3412673132790331 2216 8 -1.3746371731618368 2216 9 -1.5272782198128212 2216 10 -1.121040722493575 2216 11 -1.5278789335240575 2216 12 -2.2322803002591485 2216 13 -1.590967484696428 2216 14 -7.418713939764206 2216 15 -3.6478900043018996 2216 16 -2.1657195808730165 2216 17 -1.28070379467907 2216 18 -0.4847482863826187 2216 19 -1.0217816987767918 2216 20 -1.6564842372457917 2216 21 -1.7516443402041517 2216 22 -1.3529256486828487 2216 23 -0.2072373160216472 2216 24 -0.9785453890302804 2216 25 -0.7767271368401225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89128.0 3 2.0 4 726.0 5 4599.0 6 5885.0 7 5132.0 8 4967.0 9 4442.0 10 4254.0 11 4190.0 12 4145.0 13 3952.0 14 3991.0 15 4057.0 16 4161.0 17 4514.0 18 5033.0 19 5973.0 20 7510.0 21 9224.0 22 11244.0 23 14007.0 24 16573.0 25 19279.0 26 21906.0 27 25511.0 28 28372.0 29 34314.0 30 40649.0 31 46173.0 32 60121.0 33 78579.0 34 103322.0 35 137616.0 36 195451.0 37 275442.0 38 701515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5165485795519 18.137525488920982 12.138649648007139 25.20727628351998 2 12.984313555590706 20.228047390815032 41.9542615562718 24.833377497322452 3 19.977867124166742 25.226945088909286 28.522648167867338 26.27253961905664 4 10.681812229141777 16.708942879765644 38.800497240797114 33.80874765029546 5 11.825050106357955 38.720087927276644 36.38605595088242 13.068806015482979 6 32.39999617243965 35.27293275228281 18.664443324155584 13.662627751121953 7 27.822121900255187 31.439353306067115 22.009440941737214 18.72908385194049 8 25.755551269001785 36.61195938428747 20.801224051730085 16.831265294980653 9 27.25357404765934 15.193127513317053 19.9247112959247 37.628587143098905 10 14.339833435920578 29.95735661375448 34.8122351158665 20.890574834458437 11 33.34894585276085 23.596610773847114 23.034257896661238 20.0201854767308 12 23.69793030648547 25.90823566383697 31.243377343362837 19.15045668631472 13 29.013929877219663 21.55706414995518 24.83672934943545 24.592276623389704 14 20.48164555431997 23.163397073280795 28.154412238714567 28.200545133684667 15 25.737434193859993 28.884847501998166 22.767854219978474 22.609864084163362 16 20.82390062224416 28.188088468967116 29.909420791667824 21.078590117120903 17 22.59987025781223 28.498455785917333 28.450356488821825 20.45131746744861 18 22.05796218281078 26.68078494967529 30.762513188675495 20.49873967883844 19 23.127322949621554 27.886476611464055 28.56141955611924 20.42478088279515 20 23.444536051528733 27.489898178473993 28.09910487511414 20.966460894883138 21 21.839345030182304 27.307768826478835 28.690589215965268 22.1622969273736 22 22.269436242435596 28.358080397484294 26.193757473734262 23.17872588634585 23 19.889535598226285 28.104104085953917 29.46466166625366 22.54169864956614 24 22.082602099103983 29.4555083849311 27.270771921411548 21.191117594553372 25 22.11208937331896 26.98082986632282 26.96667640451702 23.9404043558412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 174.0 1 174.0 2 323.0 3 472.0 4 472.0 5 472.0 6 1013.0 7 1554.0 8 1554.0 9 1554.0 10 2352.0 11 3150.0 12 3150.0 13 3150.0 14 5761.5 15 8373.0 16 8373.0 17 8373.0 18 14866.5 19 21360.0 20 21360.0 21 21360.0 22 33813.0 23 46266.0 24 46266.0 25 46266.0 26 66342.0 27 86418.0 28 86418.0 29 86418.0 30 111805.0 31 137192.0 32 137192.0 33 137192.0 34 157430.0 35 177668.0 36 177668.0 37 177668.0 38 196795.5 39 215923.0 40 215923.0 41 215923.0 42 233697.5 43 251472.0 44 251472.0 45 251472.0 46 295870.5 47 340269.0 48 340269.0 49 340269.0 50 307805.0 51 275341.0 52 275341.0 53 275341.0 54 226917.5 55 178494.0 56 178494.0 57 178494.0 58 146588.0 59 114682.0 60 114682.0 61 114682.0 62 91059.5 63 67437.0 64 67437.0 65 67437.0 66 51191.5 67 34946.0 68 34946.0 69 34946.0 70 25120.0 71 15294.0 72 15294.0 73 15294.0 74 10654.5 75 6015.0 76 6015.0 77 6015.0 78 4149.0 79 2283.0 80 2283.0 81 2283.0 82 1546.0 83 809.0 84 809.0 85 809.0 86 530.5 87 252.0 88 252.0 89 252.0 90 171.0 91 90.0 92 90.0 93 90.0 94 53.5 95 17.0 96 17.0 97 17.0 98 12.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1134968043146913 2 0.04697982183922226 3 0.03408932409984295 4 0.030363164597053613 5 0.0343410916338152 6 0.018127262446002157 7 0.017724434391646556 8 0.021249179867258085 9 0.026939126135030984 10 0.033082253963953936 11 0.018781858034330013 12 0.017674080884852103 13 0.01505569853154068 14 0.01968822115663012 15 0.019738574663424574 16 0.020342816744957977 17 0.024320743781719563 18 0.017825141405235456 19 0.030514225117436965 20 0.021450593894435887 21 0.02638523756029203 22 0.033887910072665145 23 0.02633488405349758 24 0.036808413466743274 25 0.029154680433986804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1985959.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.76239792872526 #Duplication Level Percentage of deduplicated Percentage of total 1 86.5816345213228 61.26724075317299 2 9.591890700503349 13.574903732757146 3 2.0917645982443083 4.440548366225515 4 0.7436105896294107 2.1047867378948157 5 0.2999827236279116 1.0613748430550547 6 0.1674034951929304 0.7107523644900953 7 0.10686108402115511 0.5293222585419947 8 0.07058756545971367 0.39959563167041623 9 0.047901593387188794 0.3050668451415823 >10 0.2579809089874386 3.237736238843513 >50 0.020007114441145918 0.9851061379125545 >100 0.015355154430645744 2.2437618966311113 >500 0.0018915756457248995 0.8842497357161121 >1k 0.0025463518307835 3.641867172466997 >5k 3.637645472547857E-4 1.6585345532038955 >10k+ 2.182587283528714E-4 2.9551527322761983 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 25731 1.2956460833280041 No Hit CCCATGTACTCTGCGTTGATACCAC 20490 1.0317433542182894 No Hit GAGTACATGGGAAGCAGTGGTATCA 11181 0.5630025594687503 No Hit GTATCAACGCAGAGTACATGGGAAG 7257 0.3654153988073268 No Hit GCGTTGATACCACTGCTTCCCATGT 7114 0.35821484733572045 No Hit CATGTACTCTGCGTTGATACCACTG 6734 0.3390805147538293 No Hit TATCAACGCAGAGTACATGGGAAGC 5681 0.2860582720992729 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5430 0.2734195418938659 No Hit GCTTCCCATGTACTCTGCGTTGATA 4882 0.24582582017050703 No Hit ACTCTGCGTTGATACCACTGCTTCC 4633 0.2332877969786889 No Hit GGTATCAACGCAGAGTACATGGGAA 3755 0.1890774180131614 No Hit CAGTGGTATCAACGCAGAGTACATG 2761 0.13902603225947766 No Hit CCACTGCTTCCCATGTACTCTGCGT 2475 0.12462492931626483 No Hit GTCCTACAGTGGACATTTCTAAATT 2411 0.12140230488142001 No Hit GTGGTATCAACGCAGAGTACATGGG 2262 0.11389963236904689 No Hit GTATCAACGCAGAGTACTTTTTTTT 2246 0.11309397626033568 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2199 0.11072736144099651 No Hit GCAGTGGTATCAACGCAGAGTACAT 2178 0.10966993779831305 No Hit GTACTCTGCGTTGATACCACTGCTT 2153 0.10841110012845179 No Hit ATACCACTGCTTCCCATGTACTCTG 2150 0.10826003960806845 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2116 0.10654802037705713 No Hit CTGTAGGACGTGGAATATGGCAAGA 2029 0.10216726528593995 No Hit ACATGGGAAGCAGTGGTATCAACGC 2012 0.10131125567043428 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.035350679445044E-5 0.0 5 0.0 0.0 0.0 1.0070701358890088E-4 0.0 6 0.0 0.0 0.0 1.0070701358890088E-4 0.0 7 0.0 0.0 0.0 1.0070701358890088E-4 5.035350679445044E-5 8 0.0 0.0 0.0 1.0070701358890088E-4 5.035350679445044E-5 9 0.0 0.0 0.0 1.5106052038335133E-4 5.035350679445044E-5 10 5.035350679445044E-5 0.0 0.0 2.517675339722522E-4 5.035350679445044E-5 11 5.035350679445044E-5 0.0 0.0 3.0212104076670265E-4 5.035350679445044E-5 12 5.035350679445044E-5 0.0 0.0 3.0212104076670265E-4 5.035350679445044E-5 13 5.035350679445044E-5 0.0 0.0 3.0212104076670265E-4 5.035350679445044E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 90 2.2901077E-9 14.777209 19 GCGTTTA 390 0.0 14.369795 13 TCCAACG 160 0.0 14.249452 18 TATCGAC 80 1.2896271E-7 14.249452 19 TAGGACC 590 0.0 14.171087 4 TGCGTTT 430 0.0 13.916668 12 TTACGAC 60 4.0907649E-4 12.667457 3 CGTTTAT 430 0.0 12.591271 14 GTAGGAC 1310 0.0 12.111441 3 TTATCGA 55 0.0030698446 12.090445 18 GGTATCG 55 0.0030698446 12.090445 17 GTGTAGG 345 0.0 11.8541565 1 GCCTTAG 185 0.0 11.824353 1 ACGTTAA 65 7.948364E-4 11.705713 1 AGGACCT 1020 0.0 11.176886 5 GGACCGA 60 0.005880519 11.082349 6 CGAAATC 60 0.0058827237 11.081791 13 TGTAGGA 1560 0.0 11.023955 2 GTATTAG 285 0.0 11.012612 1 CGCAGAT 165 8.0035534E-11 10.937871 8 >>END_MODULE