FastQCFastQC Report
Thu 26 May 2016
SRR1513096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985959
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC299531.508238589014174No Hit
CCCATGTACTCTGCGTTGATACCAC275231.3858795675036595No Hit
GAGTACATGGGAAGCAGTGGTATCA120490.6067094033663334No Hit
CATGTACTCTGCGTTGATACCACTG80750.4066045673651873No Hit
GTATCAACGCAGAGTACATGGGAAG76190.38364336826691786No Hit
GCGTTGATACCACTGCTTCCCATGT74990.37760094745158385No Hit
ACGCAGAGTACATGGGAAGCAGTGG68150.34315914880417975No Hit
TATCAACGCAGAGTACATGGGAAGC65850.33157784224145614No Hit
ACTCTGCGTTGATACCACTGCTTCC57850.2912950368058958No Hit
GCTTCCCATGTACTCTGCGTTGATA57700.29053973420397905No Hit
GGTATCAACGCAGAGTACATGGGAA45310.2281517392856549No Hit
GTATCAACGCAGAGTACTTTTTTTT34280.17261182129137612No Hit
CAGTGGTATCAACGCAGAGTACATG33110.1667204609964254No Hit
CCACTGCTTCCCATGTACTCTGCGT31180.15700223418509648No Hit
ATACCACTGCTTCCCATGTACTCTG28750.14476633203404501No Hit
CATGGGAAGCAGTGGTATCAACGCA27610.13902603225947766No Hit
ACATGGGAAGCAGTGGTATCAACGC27120.1365587104265496No Hit
GTCCTACAGTGGACATTTCTAAATT25950.13066735013159889No Hit
GCAGTGGTATCAACGCAGAGTACAT25270.12724331166957625No Hit
GGGAAGCAGTGGTATCAACGCAGAG24680.12427245476870367No Hit
TATCAACGCAGAGTACTTTTTTTTT24440.12306397060563687No Hit
TACCACTGCTTCCCATGTACTCTGC24420.12296326359204797No Hit
GTGGTATCAACGCAGAGTACATGGG24160.12165407241539226No Hit
GTACTCTGCGTTGATACCACTGCTT23040.1160144796544138No Hit
CTGCTTCCCATGTACTCTGCGTTGA22730.11445352094378584No Hit
CTGTAGGACGTGGAATATGGCAAGA22410.11284220872636343No Hit
AAGCAGTGGTATCAACGCAGAGTAC21970.11062665442740761No Hit
GGTATCAACGCAGAGTACTTTTTTT20620.1038289310101568No Hit
CCATGTACTCTGCGTTGATACCACT20470.10307362840824005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG400.005204645514.2808185
GATCTCG551.9839451E-413.79889719
TAGGACC6400.013.5370254
TCGTCAA854.0125797E-612.27541410
CGCTCGC550.003104152212.072515510
AAGTCGT951.056007E-611.98506457
CGGTTTC1052.7510396E-711.75200113
GTGTAGG2450.011.6669511
TCCAACG1552.5465852E-1111.63271818
ATAGTAC1655.456968E-1211.5400563
GTAGGAC13850.011.4109073
GTCCTAC14700.011.342871
GCCGGTT855.3992393E-511.16115411
ACCGTCT600.005932472611.0692588
TGTAGGA15300.011.0198132
AGGACGT12050.010.9031975
CCAACGA1053.5256817E-610.8419919
GGACGTG11950.010.75515656
TAGACTG2400.010.7106135
AAGGCGT1252.175766E-710.6627436
Produced by FastQC (version 0.11.5)