##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985959 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.581256712751873 31.0 31.0 34.0 27.0 34.0 2 30.713665790683493 33.0 31.0 34.0 27.0 34.0 3 30.725102079146648 33.0 31.0 34.0 27.0 34.0 4 33.95624380966576 37.0 35.0 37.0 30.0 37.0 5 33.89015684613831 37.0 35.0 37.0 30.0 37.0 6 33.84382457039647 37.0 35.0 37.0 30.0 37.0 7 33.76512354988195 37.0 35.0 37.0 29.0 37.0 8 33.76240194283971 37.0 35.0 37.0 29.0 37.0 9 35.32548255024399 39.0 35.0 39.0 29.0 39.0 10 35.229775136344706 39.0 35.0 39.0 29.0 39.0 11 35.247551938383424 39.0 35.0 39.0 29.0 39.0 12 35.166923889163876 39.0 35.0 39.0 28.0 39.0 13 35.140885587265394 39.0 35.0 39.0 28.0 39.0 14 36.3280490684853 40.0 37.0 41.0 28.0 41.0 15 36.308031535394235 40.0 37.0 41.0 28.0 41.0 16 36.247311248620946 40.0 37.0 41.0 27.0 41.0 17 36.185782284528536 40.0 37.0 41.0 27.0 41.0 18 36.11323093779882 40.0 36.0 41.0 27.0 41.0 19 36.16044641404984 40.0 37.0 41.0 27.0 41.0 20 36.09302055077673 40.0 37.0 41.0 27.0 41.0 21 36.11136584390715 40.0 37.0 41.0 27.0 41.0 22 36.10257512869097 40.0 37.0 41.0 27.0 41.0 23 36.03875155529394 40.0 37.0 41.0 27.0 41.0 24 35.914677493342005 40.0 37.0 41.0 27.0 41.0 25 35.89298419554482 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.685086141888867 1101 2 0.6051660608687293 1101 3 0.649673899750276 1101 4 0.8495983615057057 1101 5 0.6864992650400481 1101 6 0.7325401032285157 1101 7 0.6107836843839536 1101 8 0.59170988403568 1101 9 0.6350842250810018 1101 10 0.6339691790707676 1101 11 0.6498342823287402 1101 12 0.6945831638723021 1101 13 0.7152361066130553 1101 14 0.7215583008313473 1101 15 0.6302550524453565 1101 16 0.7148683050681299 1101 17 0.6062751832178748 1101 18 0.6192308111352673 1101 19 0.6515571484767264 1101 20 0.7733466357997756 1101 21 0.6925491715874159 1101 22 0.6501425427423726 1101 23 0.6046708296438226 1101 24 0.7937118110993353 1101 25 0.8210520350538388 1102 1 0.6254263936044531 1102 2 0.5535358029343733 1102 3 0.6033714017569132 1102 4 0.7855438948375948 1102 5 0.5879812396443285 1102 6 0.432074960883746 1102 7 0.44317855281357055 1102 8 0.3774151631210856 1102 9 0.4250850205051435 1102 10 0.4408374857573918 1102 11 0.47583457338166113 1102 12 0.500771066555771 1102 13 0.5822450316334837 1102 14 0.561557378862453 1102 15 0.5553475656964011 1102 16 0.5703801876201382 1102 17 0.5758099866396478 1102 18 0.5481553705787547 1102 19 0.513890019017829 1102 20 0.6789908028099205 1102 21 0.6261222168325844 1102 22 0.6603657767199991 1102 23 0.5726339924671464 1102 24 0.6356151616427539 1102 25 0.7177250831914606 1103 1 0.06478595428862377 1103 2 0.14144609810762887 1103 3 0.3017928937195862 1103 4 -0.5581884317453998 1103 5 -0.09072306324360113 1103 6 0.07803472712335235 1103 7 0.15635734797594836 1103 8 0.20599321436855433 1103 9 0.2388706483011731 1103 10 0.3150344781949812 1103 11 0.21500371432671272 1103 12 0.24250602511840924 1103 13 0.23854216074549583 1103 14 0.4051897826646851 1103 15 0.24411166817684204 1103 16 0.23071002796120865 1103 17 0.3185670578191022 1103 18 0.37246143972078727 1103 19 0.37400809557000514 1103 20 0.46391030266128297 1103 21 0.4945540500100307 1103 22 0.38018388553749105 1103 23 0.34080249173120336 1103 24 0.41238427068502403 1103 25 0.44344044873210464 1104 1 0.3014434216523192 1104 2 0.33546638792462957 1104 3 0.36281472649945457 1104 4 0.2541155116812206 1104 5 0.18807892882809085 1104 6 0.235159037036496 1104 7 0.11761631306661258 1104 8 0.2779495804205254 1104 9 0.14223678285973307 1104 10 0.08283092196722208 1104 11 0.0895384156306065 1104 12 0.15840553284402148 1104 13 0.11900316626040564 1104 14 0.13837063033477648 1104 15 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51578.0 3 0.0 4 824.0 5 6767.0 6 8048.0 7 5934.0 8 4951.0 9 4168.0 10 3741.0 11 3388.0 12 3060.0 13 2923.0 14 2776.0 15 2754.0 16 2910.0 17 3042.0 18 3543.0 19 4007.0 20 5166.0 21 6677.0 22 8648.0 23 10531.0 24 12099.0 25 13701.0 26 14710.0 27 16194.0 28 18108.0 29 21912.0 30 27288.0 31 35003.0 32 44656.0 33 58098.0 34 78205.0 35 107563.0 36 157775.0 37 266082.0 38 969129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.49719027009629 18.95617991755704 13.093406934349838 27.45322287799683 2 12.55378122510595 20.52148956421273 40.075660276490545 26.84906893419078 3 17.826249182384934 25.223229683996497 28.94168510024628 28.00883603337229 4 10.249607368530771 17.392252307323567 37.17498699620687 35.18315332793879 5 12.099931253824478 38.611730162447465 36.15590524611383 13.13243333761423 6 32.771821429510105 35.65748852142163 18.128723840870816 13.441966208197453 7 27.46614190991049 31.261380024331096 22.18883186567946 19.083646200078956 8 25.769113370116326 37.13397920152639 20.332709090341293 16.764198338015994 9 26.531577499891707 15.048360303147224 19.915670430654014 38.50439176630706 10 14.25629260658381 30.192023574174353 34.492235769942326 21.05944804929951 11 33.211926565774675 23.288950121003925 23.03243992857596 20.466683384645442 12 23.272798873577617 25.965929348822314 31.401088552672128 19.360183224927937 13 28.99427533195823 21.253031536031973 24.99637424261957 24.75631888939023 14 20.50875212370299 23.097529706664627 27.402793065977548 28.990925103654835 15 25.564777520583252 28.99697325070659 22.70500045569924 22.73324877301092 16 20.891350033010912 28.028707155688124 29.673461391836764 21.406481419464203 17 22.735122976477502 28.74833602876108 27.89830674085263 20.618234253908792 18 22.74755573291734 26.918631250743207 29.632316929383805 20.701496086955647 19 22.907010596534846 28.01216501138833 28.577199367774003 20.503625024302824 20 23.19393300667335 27.452530490307204 28.23401691575707 21.119519587262374 21 21.508953608810653 27.47050669223282 28.579945507434946 22.440594191521576 22 22.215866490697945 28.2782272947226 25.936587814753477 23.569318399825978 23 20.42366604211282 28.419684070755018 27.724097925880347 23.432551961251814 24 21.995714907941018 29.47639800499004 27.32161604870201 21.206271038366932 25 22.090950563909207 26.82287339409998 26.61526578104874 24.470910260942073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 112.0 1 112.0 2 315.0 3 518.0 4 518.0 5 518.0 6 1130.0 7 1742.0 8 1742.0 9 1742.0 10 2449.5 11 3157.0 12 3157.0 13 3157.0 14 5351.5 15 7546.0 16 7546.0 17 7546.0 18 14050.0 19 20554.0 20 20554.0 21 20554.0 22 33092.0 23 45630.0 24 45630.0 25 45630.0 26 65211.0 27 84792.0 28 84792.0 29 84792.0 30 109994.0 31 135196.0 32 135196.0 33 135196.0 34 154992.0 35 174788.0 36 174788.0 37 174788.0 38 191698.0 39 208608.0 40 208608.0 41 208608.0 42 223765.5 43 238923.0 44 238923.0 45 238923.0 46 293819.0 47 348715.0 48 348715.0 49 348715.0 50 317295.5 51 285876.0 52 285876.0 53 285876.0 54 233940.0 55 182004.0 56 182004.0 57 182004.0 58 149423.0 59 116842.0 60 116842.0 61 116842.0 62 93237.0 63 69632.0 64 69632.0 65 69632.0 66 52814.5 67 35997.0 68 35997.0 69 35997.0 70 25858.5 71 15720.0 72 15720.0 73 15720.0 74 10909.5 75 6099.0 76 6099.0 77 6099.0 78 4208.0 79 2317.0 80 2317.0 81 2317.0 82 1591.5 83 866.0 84 866.0 85 866.0 86 552.5 87 239.0 88 239.0 89 239.0 90 159.0 91 79.0 92 79.0 93 79.0 94 43.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18832211541124463 2 0.06535885181919668 3 0.0 4 0.0 5 0.019839281677013473 6 0.37719811939722825 7 0.00115813065627236 8 0.03126952771935372 9 0.03288083993677614 10 0.002870149887283675 11 0.003675805995994882 12 0.01022176187927344 13 0.008409035634673224 14 0.002064493778572468 15 0.0 16 0.01268908371220151 17 0.02507604638363632 18 0.06621486143470233 19 0.02985962952910911 20 0.0 21 0.0 22 0.0 23 4.028280543556035E-4 24 2.0141402717780175E-4 25 5.035350679445044E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1985959.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.53058817846046 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95783692863283 55.857642730532106 2 11.112156322902438 14.78596592187395 3 2.733676592612686 5.456193347886349 4 0.9234986921036489 2.457636446707789 5 0.41069694843238064 1.3661954771152567 6 0.22892160477420362 0.9138173407430897 7 0.13561039337373745 0.6315567463987004 8 0.09048799669337836 0.4816175714480838 9 0.05946707458631856 0.3560741504532137 >10 0.30491217457947484 3.6648057014001543 >50 0.020175333895819943 0.9337518053622864 >100 0.017091978805431972 2.3731461225597292 >500 0.001974540608358037 0.8895085240505501 >1k 0.002733979303880352 3.886553518149179 >5k 5.31607086865624E-4 2.43272118239056 >10k+ 2.2783160865669602E-4 3.5128134129289625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 29953 1.508238589014174 No Hit CCCATGTACTCTGCGTTGATACCAC 27523 1.3858795675036595 No Hit GAGTACATGGGAAGCAGTGGTATCA 12049 0.6067094033663334 No Hit CATGTACTCTGCGTTGATACCACTG 8075 0.4066045673651873 No Hit GTATCAACGCAGAGTACATGGGAAG 7619 0.38364336826691786 No Hit GCGTTGATACCACTGCTTCCCATGT 7499 0.37760094745158385 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6815 0.34315914880417975 No Hit TATCAACGCAGAGTACATGGGAAGC 6585 0.33157784224145614 No Hit ACTCTGCGTTGATACCACTGCTTCC 5785 0.2912950368058958 No Hit GCTTCCCATGTACTCTGCGTTGATA 5770 0.29053973420397905 No Hit GGTATCAACGCAGAGTACATGGGAA 4531 0.2281517392856549 No Hit GTATCAACGCAGAGTACTTTTTTTT 3428 0.17261182129137612 No Hit CAGTGGTATCAACGCAGAGTACATG 3311 0.1667204609964254 No Hit CCACTGCTTCCCATGTACTCTGCGT 3118 0.15700223418509648 No Hit ATACCACTGCTTCCCATGTACTCTG 2875 0.14476633203404501 No Hit CATGGGAAGCAGTGGTATCAACGCA 2761 0.13902603225947766 No Hit ACATGGGAAGCAGTGGTATCAACGC 2712 0.1365587104265496 No Hit GTCCTACAGTGGACATTTCTAAATT 2595 0.13066735013159889 No Hit GCAGTGGTATCAACGCAGAGTACAT 2527 0.12724331166957625 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2468 0.12427245476870367 No Hit TATCAACGCAGAGTACTTTTTTTTT 2444 0.12306397060563687 No Hit TACCACTGCTTCCCATGTACTCTGC 2442 0.12296326359204797 No Hit GTGGTATCAACGCAGAGTACATGGG 2416 0.12165407241539226 No Hit GTACTCTGCGTTGATACCACTGCTT 2304 0.1160144796544138 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2273 0.11445352094378584 No Hit CTGTAGGACGTGGAATATGGCAAGA 2241 0.11284220872636343 No Hit AAGCAGTGGTATCAACGCAGAGTAC 2197 0.11062665442740761 No Hit GGTATCAACGCAGAGTACTTTTTTT 2062 0.1038289310101568 No Hit CCATGTACTCTGCGTTGATACCACT 2047 0.10307362840824005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.035350679445044E-5 10 0.0 0.0 0.0 0.0 5.035350679445044E-5 11 0.0 0.0 0.0 0.0 5.035350679445044E-5 12 0.0 0.0 0.0 5.035350679445044E-5 5.035350679445044E-5 13 0.0 0.0 0.0 5.035350679445044E-5 5.035350679445044E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGCG 40 0.0052046455 14.280818 5 GATCTCG 55 1.9839451E-4 13.798897 19 TAGGACC 640 0.0 13.537025 4 TCGTCAA 85 4.0125797E-6 12.275414 10 CGCTCGC 55 0.0031041522 12.0725155 10 AAGTCGT 95 1.056007E-6 11.9850645 7 CGGTTTC 105 2.7510396E-7 11.752001 13 GTGTAGG 245 0.0 11.666951 1 TCCAACG 155 2.5465852E-11 11.632718 18 ATAGTAC 165 5.456968E-12 11.540056 3 GTAGGAC 1385 0.0 11.410907 3 GTCCTAC 1470 0.0 11.34287 1 GCCGGTT 85 5.3992393E-5 11.161154 11 ACCGTCT 60 0.0059324726 11.069258 8 TGTAGGA 1530 0.0 11.019813 2 AGGACGT 1205 0.0 10.903197 5 CCAACGA 105 3.5256817E-6 10.84199 19 GGACGTG 1195 0.0 10.7551565 6 TAGACTG 240 0.0 10.710613 5 AAGGCGT 125 2.175766E-7 10.662743 6 >>END_MODULE