FastQCFastQC Report
Thu 26 May 2016
SRR1513095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730612
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC161390.9325602734755104No Hit
CCCATGTACTCTGCGTTGATACCAC129790.7499659080140436No Hit
GAGTACATGGGAAGCAGTGGTATCA69610.4022276512586299No Hit
GTATCAACGCAGAGTACATGGGAAG44620.25782786667375474No Hit
GCGTTGATACCACTGCTTCCCATGT43600.2519339979151884No Hit
CATGTACTCTGCGTTGATACCACTG42060.2430354117502941No Hit
ACGCAGAGTACATGGGAAGCAGTGG34150.19732903735788263No Hit
TATCAACGCAGAGTACATGGGAAGC34150.19732903735788263No Hit
GCTTCCCATGTACTCTGCGTTGATA31380.1813231388664819No Hit
ACTCTGCGTTGATACCACTGCTTCC29380.16976653345752832No Hit
GTCCTACAGTGGACATTTCTAAATT27400.15832549410266425No Hit
GGTATCAACGCAGAGTACATGGGAA24660.14249294469239784No Hit
GTATCAACGCAGAGTACTTTTTTTT23200.1340566227438617No Hit
CTGTAGGACGTGGAATATGGCAAGA21450.12394459301102731No Hit
GTCCTACAGTGTGCATTTCTCATTT21200.12250001733490812No Hit
GTCCTAAAGTGTGTATTTCTCATTT18850.10892100597938764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC350.002172252416.283567
GCGTTTA2750.014.16094413
TAGGACC6700.014.0395154
TATCGAC707.267103E-613.57080919
ACCGTAT655.459961E-513.1521048
CGTTTAT3200.012.1695614
GTACCGT550.00307106512.0896596
GTAGGAC16000.011.8761743
TGTAGGA16400.011.8192662
GACAGTC1850.011.8092477
GTCCTAC16050.011.4992951
TAGGACA5250.011.4017874
ATACGAC752.0749526E-411.3994819
CCGTATG752.0766245E-411.3984919
TCCAACG1701.0913936E-1111.1759618
TGGACTG2300.011.1532765
CTGTAGG16000.011.119011
AGCGCCA600.005883171711.08154710
AAGTCGT951.362327E-510.9985457
AGGACCT11200.010.9430475
Produced by FastQC (version 0.11.5)