##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513095_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1730612
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.331088077512465	31.0	30.0	34.0	25.0	34.0
2	29.089771710816752	31.0	30.0	34.0	16.0	34.0
3	29.426136534358942	31.0	30.0	34.0	23.0	34.0
4	32.26088169965307	35.0	33.0	37.0	19.0	37.0
5	32.50012770048977	35.0	33.0	37.0	22.0	37.0
6	32.979330433395816	37.0	35.0	37.0	27.0	37.0
7	32.975264819612946	37.0	35.0	37.0	26.0	37.0
8	32.95648706931421	37.0	35.0	37.0	26.0	37.0
9	34.32764594259141	39.0	35.0	39.0	26.0	39.0
10	33.8266214495219	39.0	34.0	39.0	21.0	39.0
11	34.19378000383679	39.0	35.0	39.0	26.0	39.0
12	34.04993435848128	39.0	35.0	39.0	25.0	39.0
13	34.12407518265215	39.0	35.0	39.0	25.0	39.0
14	34.484399160528184	40.0	34.0	41.0	17.0	41.0
15	34.998162499739976	40.0	34.0	41.0	24.0	41.0
16	34.56201967858769	39.0	34.0	41.0	17.0	41.0
17	34.115575299373866	39.0	33.0	41.0	16.0	41.0
18	33.5344011251511	39.0	32.0	41.0	10.0	41.0
19	34.41524096677938	39.0	34.0	41.0	18.0	41.0
20	34.695288718672934	40.0	34.0	41.0	18.0	41.0
21	34.71215327294622	40.0	35.0	41.0	18.0	41.0
22	34.31630313438252	40.0	34.0	41.0	11.0	41.0
23	32.63414156379362	39.0	31.0	41.0	2.0	41.0
24	33.75052235856448	39.0	33.0	41.0	2.0	41.0
25	33.48178216723333	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.664076177502718
1101	2	0.9535572069387221
1101	3	0.7509838915815763
1101	4	0.7332908974867252
1101	5	0.9572439868769891
1101	6	0.7418603990230324
1101	7	0.7251290913770063
1101	8	0.7009716046551802
1101	9	0.836574857327868
1101	10	1.1708084074939364
1101	11	0.83647644426987
1101	12	0.740699951334733
1101	13	0.6263321517138394
1101	14	-0.3530971798621607
1101	15	0.2536149115902404
1101	16	1.0026195792205428
1101	17	1.511095371218758
1101	18	-4.767982915629958
1101	19	-0.9195246033621771
1101	20	-0.023296169253107735
1101	21	0.33719505689138174
1101	22	0.9022352372778215
1101	23	2.790702398458386
1101	24	1.6356702517466886
1101	25	1.7963872096977909
1102	1	0.6369414492917898
1102	2	0.9796510214257772
1102	3	0.7342959239969744
1102	4	1.2690604781797674
1102	5	0.9837553639613787
1102	6	0.6381962801057597
1102	7	0.6119548061643698
1102	8	0.4610620832018739
1102	9	0.5849453656084336
1102	10	1.0603834798334546
1102	11	0.6846526790580469
1102	12	0.7528759626627917
1102	13	0.7123027325049378
1102	14	1.55115329385049
1102	15	1.0411126028823645
1102	16	1.2640542387972928
1102	17	0.9430757067281661
1102	18	1.4281073303357914
1102	19	1.245374144121044
1102	20	0.965257485117931
1102	21	1.009483379731968
1102	22	1.3812944017649968
1102	23	2.8042938472324295
1102	24	1.667564623515922
1102	25	1.9422944121823065
1103	1	0.441777057744968
1103	2	0.7669769213631632
1103	3	0.5543432478369112
1103	4	0.900307796182112
1103	5	0.8878556875832544
1103	6	0.5427658568433813
1103	7	0.4305533952055711
1103	8	0.40578058605991174
1103	9	0.4423206241256423
1103	10	0.681744487874461
1103	11	0.4152571876487201
1103	12	0.5062080947979553
1103	13	0.3341851952642827
1103	14	0.9970932019476066
1103	15	0.602256255798963
1103	16	0.8421519057505051
1103	17	1.291965538613752
1103	18	1.0855066689470974
1103	19	0.6140510495988138
1103	20	0.5647503718076123
1103	21	0.5152627426422853
1103	22	0.9596838083197738
1103	23	2.2602852300388534
1103	24	1.0544357520724716
1103	25	1.2643735290434464
1104	1	0.530624274847554
1104	2	1.0190253947766479
1104	3	0.7258065680062487
1104	4	1.2123347794883799
1104	5	1.1333811245528906
1104	6	0.720237363484614
1104	7	0.5685720831875187
1104	8	0.6355148624510534
1104	9	0.7194466617627455
1104	10	1.0957191873826773
1104	11	0.7181746524977726
1104	12	0.8694278780112086
1104	13	0.6393176420001296
1104	14	1.6058847393863758
1104	15	0.9814123361551452
1104	16	1.2302704438404675
1104	17	1.7794681259204026
1104	18	2.3655898640845834
1104	19	1.6288373868604609
1104	20	1.1153514928226471
1104	21	1.2301247818165137
1104	22	1.4787518116722111
1104	23	3.0169755995955754
1104	24	1.9265110647551822
1104	25	2.027650707170821
1105	1	0.6668845941354391
1105	2	1.109101185881471
1105	3	0.8133683430590288
1105	4	0.989510933208841
1105	5	1.0143680429043158
1105	6	0.6418110460947588
1105	7	0.4750781716891055
1105	8	0.5841226716184877
1105	9	0.7938767405729408
1105	10	1.2545712940918747
1105	11	0.7997006789077901
1105	12	0.7222552736171863
1105	13	0.8066426834720559
1105	14	1.5500418230520125
1105	15	0.8745256689618444
1105	16	1.3542474424737634
1105	17	1.7615653991070843
1105	18	1.572669796430958
1105	19	1.1841322136089616
1105	20	1.0795655172069516
1105	21	0.9325010450466777
1105	22	1.3943779377447214
1105	23	2.925283133581999
1105	24	1.971442507567204
1105	25	1.9503879304072171
1106	1	-0.6571076704647858
1106	2	-0.21347585842502426
1106	3	-0.4033874688138468
1106	4	-2.6818651971642993
1106	5	-0.6971022336155812
1106	6	-0.7727393509982718
1106	7	-0.6923428570364791
1106	8	-0.6471839502691097
1106	9	-0.7081453369737574
1106	10	-0.5844185617984508
1106	11	-0.6274352962067979
1106	12	-0.46950528685519544
1106	13	-0.48707636460579096
1106	14	0.14442305989012993
1106	15	-0.3877027317924231
1106	16	-1.4489873860949132
1106	17	-5.987966072408202
1106	18	-6.978885679598939
1106	19	-3.135866960547645
1106	20	-1.7812311463857426
1106	21	-1.282664330092679
1106	22	-1.6027742789565878
1106	23	-5.076465314520622
1106	24	-2.442611144725202
1106	25	-1.0834862385878665
1107	1	0.5536158258425274
1107	2	0.8726859438091772
1107	3	0.6256760927281277
1107	4	0.953332450331942
1107	5	0.948589067866564
1107	6	0.44840208653024405
1107	7	0.35916683521141124
1107	8	0.4353334991571245
1107	9	0.34364557411444707
1107	10	0.715971156137698
1107	11	0.49796599834628097
1107	12	0.6875709836151387
1107	13	0.5633949811548007
1107	14	1.2213026123736128
1107	15	0.7355461570213535
1107	16	-5.881786561366585
1107	17	-0.9570705323632467
1107	18	0.8299990781371136
1107	19	0.7871392418149981
1107	20	0.6558802764396319
1107	21	0.7055244786142865
1107	22	0.8412506656953553
1107	23	2.467824286804351
1107	24	1.4151516106786275
1107	25	1.1843936349988553
1108	1	0.3083159656813983
1108	2	0.6411828577669461
1108	3	0.3054146634624608
1108	4	0.9080973854870393
1108	5	0.6872576563534949
1108	6	0.17411161177231804
1108	7	0.1763772646126185
1108	8	0.20977174690664668
1108	9	0.37270825697112997
1108	10	0.7952469031847187
1108	11	0.2648827000002143
1108	12	0.32045592895235586
1108	13	0.1601029823845863
1108	14	0.8827708874627902
1108	15	0.5630061475665258
1108	16	0.8853965944959015
1108	17	0.8830701660376974
1108	18	0.742276137619875
1108	19	0.6853669576809267
1108	20	0.48704748472020754
1108	21	0.5506914422594846
1108	22	0.9282992059825048
1108	23	2.504146689784932
1108	24	1.276514758673116
1108	25	1.5783511411725968
1109	1	0.09252748736210492
1109	2	0.5004946624630797
1109	3	0.41636998153132154
1109	4	0.405635184469773
1109	5	0.22242555782563755
1109	6	0.18268104013424846
1109	7	0.15336774560612554
1109	8	0.11362613165500335
1109	9	0.10135718181112452
1109	10	0.5937472200425518
1109	11	0.1799402486818522
1109	12	0.2617924386057311
1109	13	0.04712110134396852
1109	14	-1.0307100031897392
1109	15	-0.3693415533064055
1109	16	-0.4092825164441507
1109	17	0.4351599346817068
1109	18	1.1794337735618399
1109	19	0.4230318979424439
1109	20	0.10031370833888786
1109	21	0.1879844478302104
1109	22	0.05275432663732005
1109	23	-4.04647192951121
1109	24	-1.5639375351453353
1109	25	-0.28027983392005495
1110	1	0.812285193987087
1110	2	1.1296321298415677
1110	3	0.8416995084697341
1110	4	1.3840751807605258
1110	5	1.1391178359662462
1110	6	0.733017521912565
1110	7	0.6017065324170972
1110	8	0.6157132459925663
1110	9	0.7747545879870188
1110	10	1.0470597109350237
1110	11	0.7669797389252082
1110	12	0.8329907906398617
1110	13	0.6713014237835964
1110	14	1.5737709008069771
1110	15	1.0065629070642501
1110	16	1.3451738800214628
1110	17	1.7108080595243962
1110	18	2.3924923134428795
1110	19	1.5494424057119929
1110	20	1.2575354816243518
1110	21	1.0315985800649017
1110	22	1.3208971856726492
1110	23	2.7275997649527355
1110	24	1.7191747888309763
1110	25	2.0439051250795046
1111	1	0.5633078866361529
1111	2	0.8406035553850408
1111	3	0.7183663517672549
1111	4	1.1432032425460008
1111	5	0.9178883527439652
1111	6	0.48612931599631537
1111	7	0.4259630269128394
1111	8	0.12052268337913574
1111	9	0.4058943868927898
1111	10	0.6703352418211352
1111	11	0.4024447858869351
1111	12	0.5960937090231582
1111	13	0.5589178345563113
1111	14	-0.20566463113892297
1111	15	0.3707673396155542
1111	16	0.648430732194683
1111	17	1.2275374301445012
1111	18	1.9086533742877947
1111	19	0.9863712808825511
1111	20	0.7366113489922483
1111	21	0.5912512354890112
1111	22	0.924260678724437
1111	23	0.9491723536103862
1111	24	0.7983129185751707
1111	25	0.35565483032677037
1112	1	0.4115735936980478
1112	2	0.6334069101827602
1112	3	0.41375649932732017
1112	4	0.9279320647995277
1112	5	0.8226332147009643
1112	6	0.16323042601670323
1112	7	0.21033108603060668
1112	8	0.22138039089931283
1112	9	0.276952746937269
1112	10	0.6761117483424712
1112	11	0.2344330581556946
1112	12	0.32600202753625496
1112	13	0.11815446444796152
1112	14	0.5751684439794857
1112	15	0.26130690899117326
1112	16	0.706261544569216
1112	17	1.0258317795340304
1112	18	1.0116730567745762
1112	19	0.4297741716745591
1112	20	0.2689174209053178
1112	21	0.4814901906169027
1112	22	0.3671140274031117
1112	23	2.00169730498353
1112	24	0.9791036811641263
1112	25	0.9728812375113023
1113	1	0.12809851858805743
1113	2	0.5676791789716766
1113	3	0.31237513989356813
1113	4	0.5102099478916244
1113	5	0.5786331897854211
1113	6	0.1996299634180545
1113	7	0.07924728051305863
1113	8	0.048347109957838086
1113	9	0.12246234253718313
1113	10	0.3901850872541246
1113	11	0.02028289630845137
1113	12	0.014885748756206851
1113	13	0.05024973734587235
1113	14	0.6965813838686543
1113	15	0.2614880657784795
1113	16	0.6521044894207293
1113	17	0.963754175911312
1113	18	1.5113917187266637
1113	19	0.6262088607596823
1113	20	0.40889815288255704
1113	21	0.3225352316964063
1113	22	0.6470282556336713
1113	23	0.5872567732027107
1113	24	0.5400289407529755
1113	25	0.926319271508568
1114	1	-0.1881325914780696
1114	2	-2.983259535940416
1114	3	-0.9507527769863593
1114	4	0.1795446618365375
1114	5	0.466905683290058
1114	6	0.2744865161979533
1114	7	0.20261240316383322
1114	8	0.2593989501429803
1114	9	0.3045978310483122
1114	10	0.422791126796632
1114	11	0.19965776058738527
1114	12	0.34606953420217934
1114	13	0.18028271082693692
1114	14	1.074368125582545
1114	15	0.3676903452250144
1114	16	0.7257186971254228
1114	17	1.0952130227173313
1114	18	1.2439969625522878
1114	19	0.7171942098150126
1114	20	0.4106031688251264
1114	21	0.24700835160991375
1114	22	0.561314530954796
1114	23	2.1730335135253824
1114	24	1.0527213951494119
1114	25	1.040423147984555
1115	1	0.03662073558168544
1115	2	0.31110394065838776
1115	3	0.06483349363614721
1115	4	0.3109575467766348
1115	5	0.33407745621003215
1115	6	-0.12080839931050491
1115	7	-0.21269745420558905
1115	8	-0.13799693951670378
1115	9	-0.1774459234984107
1115	10	0.05250874251068893
1115	11	-0.023181831197092606
1115	12	-0.058042246413450016
1115	13	-0.23265760628469678
1115	14	0.5476280425691726
1115	15	0.04051077234072409
1115	16	0.47940086509334634
1115	17	0.8596346066911735
1115	18	1.293082105706219
1115	19	0.5801615511583122
1115	20	0.05860699505404199
1115	21	0.0949433166447946
1115	22	0.43328014058681674
1115	23	2.026715395916767
1115	24	0.7750373264834067
1115	25	0.40987971942966084
1116	1	0.30776480951558227
1116	2	0.31714422573066514
1116	3	0.3421569108447038
1116	4	1.1391007328431826
1116	5	0.5070596334849284
1116	6	0.3103741721009996
1116	7	0.21141153184515105
1116	8	0.29866538491675243
1116	9	0.27224250755593005
1116	10	0.8684622466662049
1116	11	0.5188461384775422
1116	12	0.6625800405246949
1116	13	0.33861083430802097
1116	14	1.0213415207038992
1116	15	0.3798443633320119
1116	16	0.8601216165057579
1116	17	1.1070698501178953
1116	18	1.723341081594441
1116	19	0.8786785947572113
1116	20	0.6990525920903536
1116	21	0.6122417567468119
1116	22	0.9443343040531431
1116	23	2.361514473863039
1116	24	1.41685423308477
1116	25	1.6569533818802569
1201	1	1.0213370362431355
1201	2	1.3118390172301915
1201	3	1.0589826133029767
1201	4	1.6578465256586483
1201	5	1.5510046848240222
1201	6	1.1061241266862964
1201	7	0.9745255398603092
1201	8	0.9845604208124428
1201	9	1.1180642824252374
1201	10	1.4981080987014792
1201	11	1.1690088363567597
1201	12	1.2940728375356656
1201	13	1.2070498550079733
1201	14	2.020630304741438
1201	15	1.6305176333208635
1201	16	1.9181310116878976
1201	17	2.200841940095387
1201	18	2.7114041215415554
1201	19	1.8668201768465167
1201	20	1.5044584116294217
1201	21	1.4129451666804158
1201	22	1.7368384925658518
1201	23	3.284001089173749
1201	24	2.239042532811226
1201	25	2.426469779118463
1202	1	0.9914636074733068
1202	2	1.324321149093084
1202	3	1.0915781742323425
1202	4	1.6107964386836215
1202	5	1.4408218682707954
1202	6	1.1087060662585841
1202	7	0.9548980071771069
1202	8	0.9833514558458987
1202	9	1.1055305231802635
1202	10	1.5820465206406666
1202	11	1.1984150515804757
1202	12	1.2159610363005129
1202	13	1.2003464245110536
1202	14	2.044196778489983
1202	15	1.5232724433313862
1202	16	1.6614761779601537
1202	17	2.041013833606634
1202	18	2.783418187008209
1202	19	2.096644031920185
1202	20	1.728535226328333
1202	21	1.5600857727145723
1202	22	1.9807547689252303
1202	23	3.5290531679388764
1202	24	2.4377389271520045
1202	25	2.6557515466802926
1203	1	0.9891447470065309
1203	2	1.3199779991370377
1203	3	1.114952622283873
1203	4	1.728851924345122
1203	5	1.4911818813521336
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2211	20	-0.6714100090546751
2211	21	-0.5087212981572691
2211	22	-0.31497389050720415
2211	23	-4.543640529555102
2211	24	-1.9340580848403803
2211	25	-1.1946623469205804
2212	1	0.21982339314292432
2212	2	0.3577469230646706
2212	3	0.1188998817461453
2212	4	0.7450313261452521
2212	5	0.5397000077270278
2212	6	0.09128806150078361
2212	7	-0.07984819601895055
2212	8	-0.06654244834982848
2212	9	-0.12258125888384086
2212	10	-3.7305115253285663
2212	11	-1.1400178530128784
2212	12	-0.34732450993882935
2212	13	-0.3074127033341867
2212	14	0.5820052390190043
2212	15	0.14452769251076347
2212	16	0.4833931477346667
2212	17	0.9333990590578054
2212	18	1.486089496707656
2212	19	0.8055456895790485
2212	20	0.31667404976607116
2212	21	0.13350464956081964
2212	22	0.3913161026347396
2212	23	1.6536398170827198
2212	24	0.7883773395531293
2212	25	0.7638740723434836
2213	1	-1.5670931718908179
2213	2	-4.166911748181409
2213	3	-1.891553637446556
2213	4	-3.5878260850487997
2213	5	-1.4846239304252151
2213	6	-1.1632209350865992
2213	7	-1.0283988365070442
2213	8	-0.9375642485449411
2213	9	-0.9217071533115373
2213	10	-1.1168697737567115
2213	11	-1.0883434650981627
2213	12	-0.8764267563162988
2213	13	-1.260835034821092
2213	14	-6.106541537723391
2213	15	-2.75776583121997
2213	16	-2.0839633919760274
2213	17	-0.8802204304889472
2213	18	-0.11354938755818011
2213	19	-0.8699692401591079
2213	20	-0.8923765337290988
2213	21	-1.1007622260977428
2213	22	-1.1360924103422576
2213	23	-5.76738457856484
2213	24	-3.210828773085229
2213	25	-2.3261792976508353
2214	1	0.16981345049381247
2214	2	0.2588354980905301
2214	3	0.11212279277447124
2214	4	0.4775975370676093
2214	5	0.3372175629641134
2214	6	0.050243118089490224
2214	7	-0.10147543581823726
2214	8	-0.19702903685021766
2214	9	-0.1206905731680763
2214	10	0.33690675638123224
2214	11	-0.05636351540693596
2214	12	0.05811720477518634
2214	13	-0.10458688114039916
2214	14	0.4829390040971475
2214	15	0.0766186078832618
2214	16	0.5323655736302797
2214	17	0.6723805203687618
2214	18	1.2087663321285902
2214	19	0.23546768329909185
2214	20	0.19853296120159314
2214	21	-0.04241958165087567
2214	22	0.5539016239222931
2214	23	1.88694157305477
2214	24	0.9358336828841445
2214	25	0.7029393472854295
2215	1	-2.5764976096628054
2215	2	-4.1544843088139665
2215	3	-2.5041852355673093
2215	4	-4.063045091361612
2215	5	-4.967474518183337
2215	6	-2.4885986274975025
2215	7	-1.8234234994898912
2215	8	-2.1752166589035227
2215	9	-1.8796959356115508
2215	10	-3.4950258902970326
2215	11	-2.2246860064410825
2215	12	-2.090397597890078
2215	13	-2.0524290457196486
2215	14	-1.212166498760574
2215	15	-1.8206547057993276
2215	16	-2.4876125436961516
2215	17	-7.030970684404661
2215	18	-3.1246769118677697
2215	19	-2.278356911925812
2215	20	-2.072286111771078
2215	21	-1.8869064450930253
2215	22	-1.6894027811605383
2215	23	-6.381151774475917
2215	24	-3.7785467282036542
2215	25	-3.4631942442368207
2216	1	-0.7153882775249407
2216	2	-0.35020249810383675
2216	3	-0.5214528393970816
2216	4	-2.9160529793289918
2216	5	-0.8690323650564338
2216	6	-0.7306546224594541
2216	7	-0.6909558145389383
2216	8	-0.703003331547535
2216	9	-0.710091294626146
2216	10	-0.27835762255444507
2216	11	-0.8004675983375051
2216	12	-1.727463039199975
2216	13	-0.9445058546685985
2216	14	-6.402430356072685
2216	15	-2.880099986361202
2216	16	-1.3088791014896515
2216	17	-0.3179902282047635
2216	18	0.10958184334906917
2216	19	-0.2765103376945959
2216	20	-0.7274196948722533
2216	21	-0.9185250261128033
2216	22	-0.421145178718362
2216	23	0.8247919530423076
2216	24	0.01802590193713627
2216	25	0.11378815460186331
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80523.0
3	2.0
4	700.0
5	4045.0
6	5151.0
7	4492.0
8	4487.0
9	4128.0
10	3808.0
11	3722.0
12	3609.0
13	3659.0
14	3523.0
15	3678.0
16	3770.0
17	3970.0
18	4556.0
19	5276.0
20	6348.0
21	8327.0
22	9940.0
23	12429.0
24	14396.0
25	16822.0
26	19679.0
27	22138.0
28	24576.0
29	29997.0
30	35591.0
31	40327.0
32	52320.0
33	68856.0
34	89637.0
35	120011.0
36	169842.0
37	240405.0
38	605872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.709472940117536	17.952707897192287	12.135398619658783	25.202420543031394
2	13.281319552918896	19.736292908189277	42.56936359043079	24.413023948461035
3	20.020728479462562	24.579028628538826	28.4941447536889	26.90609813830971
4	10.646662990540333	16.474190240693794	38.84775403709189	34.03139273167399
5	11.960152094419698	38.496154286355086	36.45722662369117	13.086466995534046
6	32.60100146104196	35.71403802407945	18.353877309463485	13.331083205415103
7	28.37505591716284	31.18940862470943	21.584487220833203	18.851048237294524
8	25.484307612637135	37.228890037117054	20.3325392064048	16.954263143841008
9	27.05737376119448	15.24131058774841	19.683843768026478	38.01747188303064
10	14.229760108023934	29.727217603369272	34.91211205633337	21.130910232273422
11	34.024642781389666	23.012547948467354	23.35573673755652	19.607072532586464
12	23.74536844118968	25.44633749246496	31.470036971557274	19.33825709478809
13	29.338089282700814	21.508457375612892	24.929377388281036	24.224075953405258
14	20.414175192488415	22.99943244670952	28.027365084722643	28.55902727607942
15	24.93947346975812	29.84205017474525	22.61851438505681	22.599961970439818
16	21.07698789700578	28.118293894521724	29.63410130311929	21.17061690535321
17	22.179586325841516	28.537690864512637	28.448276858591782	20.834445951054068
18	21.673814905595275	26.8859705143091	30.899868979455725	20.540345600639903
19	23.25481249158251	27.50682213997569	28.21490275596536	21.023462612476436
20	23.289405925200686	27.53792474062292	28.33112646319536	20.841542870981037
21	22.107701102188713	27.164047458446117	28.26450217125801	22.463749268107158
22	22.43459351475645	28.276364763012946	26.639302330824066	22.649739391406534
23	20.27817948726841	27.972679130642863	29.532236753123797	22.216904628964933
24	22.292103343387737	28.65481796289081	27.79570408376794	21.257374609953512
25	22.136798328386504	27.113258750393772	27.48804235679006	23.26190056442966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	169.0
2	309.0
3	449.0
4	449.0
5	449.0
6	881.5
7	1314.0
8	1314.0
9	1314.0
10	2060.0
11	2806.0
12	2806.0
13	2806.0
14	4861.5
15	6917.0
16	6917.0
17	6917.0
18	12618.0
19	18319.0
20	18319.0
21	18319.0
22	29452.0
23	40585.0
24	40585.0
25	40585.0
26	58291.5
27	75998.0
28	75998.0
29	75998.0
30	98924.5
31	121851.0
32	121851.0
33	121851.0
34	139955.5
35	158060.0
36	158060.0
37	158060.0
38	173602.0
39	189144.0
40	189144.0
41	189144.0
42	204429.5
43	219715.0
44	219715.0
45	219715.0
46	249264.0
47	278813.0
48	278813.0
49	278813.0
50	256209.0
51	233605.0
52	233605.0
53	233605.0
54	195935.0
55	158265.0
56	158265.0
57	158265.0
58	131527.0
59	104789.0
60	104789.0
61	104789.0
62	83974.0
63	63159.0
64	63159.0
65	63159.0
66	48134.5
67	33110.0
68	33110.0
69	33110.0
70	23930.0
71	14750.0
72	14750.0
73	14750.0
74	10186.0
75	5622.0
76	5622.0
77	5622.0
78	3841.0
79	2060.0
80	2060.0
81	2060.0
82	1415.0
83	770.0
84	770.0
85	770.0
86	508.5
87	247.0
88	247.0
89	247.0
90	157.5
91	68.0
92	68.0
93	68.0
94	43.5
95	19.0
96	19.0
97	19.0
98	13.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11030779862846206
2	0.047035384014441135
3	0.036114391902979985
4	0.03062500433372703
5	0.0366344391463829
6	0.019646229195221115
7	0.021610852114743225
8	0.02369104108835487
9	0.02993160800918981
10	0.036114391902979985
11	0.021321936979519385
12	0.020975238817250778
13	0.016641511788893178
14	0.021841984222922297
15	0.021899767249967065
16	0.021899767249967065
17	0.026464626386503737
18	0.020166276438624026
19	0.03368750476709973
20	0.023633258061310104
21	0.029411560765786902
22	0.03599882584889045
23	0.0292382116846526
24	0.0398702886608899
25	0.031607315793488085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1730612.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.39097343003526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.93978897900878	64.5398671640134
2	8.632968429134696	12.671639132099152
3	1.8194192273182557	4.005868445106282
4	0.6181792444733106	1.8147510602456003
5	0.30141426984613245	1.106054333485549
6	0.15516887746813468	0.6832796978059361
7	0.10567650550023658	0.5428991125142785
8	0.0672046290136999	0.3945770513855861
9	0.053238697399028756	0.3516515843635626
>10	0.2659039452713801	3.554856554451187
>50	0.02079259050486625	1.06372784810984
>100	0.015264311166928191	2.210852222274825
>500	0.0021688376637346643	1.1168056835923033
>1k	0.0025704742681299723	3.816204725294583
>5k	8.032732087906164E-5	0.41037185195766596
>10k+	1.6065464175812327E-4	1.7165935333002897
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	16139	0.9325602734755104	No Hit
CCCATGTACTCTGCGTTGATACCAC	12979	0.7499659080140436	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6961	0.4022276512586299	No Hit
GTATCAACGCAGAGTACATGGGAAG	4462	0.25782786667375474	No Hit
GCGTTGATACCACTGCTTCCCATGT	4360	0.2519339979151884	No Hit
CATGTACTCTGCGTTGATACCACTG	4206	0.2430354117502941	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3415	0.19732903735788263	No Hit
TATCAACGCAGAGTACATGGGAAGC	3415	0.19732903735788263	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3138	0.1813231388664819	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2938	0.16976653345752832	No Hit
GTCCTACAGTGGACATTTCTAAATT	2740	0.15832549410266425	No Hit
GGTATCAACGCAGAGTACATGGGAA	2466	0.14249294469239784	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2320	0.1340566227438617	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2145	0.12394459301102731	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2120	0.12250001733490812	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1885	0.10892100597938764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.778302704476798E-5	0.0
2	0.0	0.0	0.0	5.778302704476798E-5	0.0
3	0.0	0.0	0.0	5.778302704476798E-5	0.0
4	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
5	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
6	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
7	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
8	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
9	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
10	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
11	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
12	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
13	0.0	1.1556605408953596E-4	0.0	5.778302704476798E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAC	35	0.0021722524	16.28356	7
GCGTTTA	275	0.0	14.160944	13
TAGGACC	670	0.0	14.039515	4
TATCGAC	70	7.267103E-6	13.570809	19
ACCGTAT	65	5.459961E-5	13.152104	8
CGTTTAT	320	0.0	12.16956	14
GTACCGT	55	0.003071065	12.089659	6
GTAGGAC	1600	0.0	11.876174	3
TGTAGGA	1640	0.0	11.819266	2
GACAGTC	185	0.0	11.809247	7
GTCCTAC	1605	0.0	11.499295	1
TAGGACA	525	0.0	11.401787	4
ATACGAC	75	2.0749526E-4	11.39948	19
CCGTATG	75	2.0766245E-4	11.398491	9
TCCAACG	170	1.0913936E-11	11.17596	18
TGGACTG	230	0.0	11.153276	5
CTGTAGG	1600	0.0	11.11901	1
AGCGCCA	60	0.0058831717	11.081547	10
AAGTCGT	95	1.362327E-5	10.998545	7
AGGACCT	1120	0.0	10.943047	5
>>END_MODULE