FastQCFastQC Report
Thu 26 May 2016
SRR1513095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730612
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC188931.0916947299568014No Hit
CCCATGTACTCTGCGTTGATACCAC172720.9980284431172326No Hit
GAGTACATGGGAAGCAGTGGTATCA72190.41713567223618No Hit
CATGTACTCTGCGTTGATACCACTG50290.29059084300813814No Hit
GTATCAACGCAGAGTACATGGGAAG47620.27516277478718515No Hit
GCGTTGATACCACTGCTTCCCATGT46740.2700778684072455No Hit
ACGCAGAGTACATGGGAAGCAGTGG44890.2593880084039634No Hit
TATCAACGCAGAGTACATGGGAAGC41500.2397995622357871No Hit
GTATCAACGCAGAGTACTTTTTTTT37940.21922880460784971No Hit
ACTCTGCGTTGATACCACTGCTTCC36310.20981017119955253No Hit
GCTTCCCATGTACTCTGCGTTGATA36140.20882785973979148No Hit
GTCCTACAGTGGACATTTCTAAATT29470.17028658070093122No Hit
GGTATCAACGCAGAGTACATGGGAA29140.1683797408084539No Hit
TATCAACGCAGAGTACTTTTTTTTT26530.15329837074976946No Hit
CTGTAGGACGTGGAATATGGCAAGA24450.1412795011244577No Hit
GGTATCAACGCAGAGTACTTTTTTT23440.13544341539293614No Hit
GTCCTACAGTGTGCATTTCTCATTT21900.1265448292280419No Hit
ACATGGGAAGCAGTGGTATCAACGC19340.11175237430458126No Hit
CTTTAGGACGTGAAATATGGCGAGG19250.11123232706117836No Hit
GTCCTAAAGTGTGTATTTCTCATTT19250.11123232706117836No Hit
CAGTGGTATCAACGCAGAGTACATG19130.11053893073664114No Hit
CATGGGAAGCAGTGGTATCAACGCA18530.10707194911395507No Hit
ATACCACTGCTTCCCATGTACTCTG17740.10250708997741839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.015.3825254
TGGTCGG400.00534189414.22475210
AAGCCGC500.0015099413.28795416
CGTGCGC655.5446602E-513.13054110
TCCAACG1251.05501385E-1012.9049418
GTAGGAC15450.012.3885153
TGTAGGA16900.012.3487852
CCAACGA854.010026E-612.27588319
TCCGTGT550.003102986112.0729768
AGGACCT11100.011.8388035
CTGTAGG16600.011.8294531
TGGCGAG13500.011.80844118
GGCGAGA8150.011.639153519
GGCGAGG5950.011.63817419
TTAGGAC8450.011.2692493
AGGACGT12800.011.2335925
GGACCTG10950.011.2183076
GGACGTG12650.011.2162446
ATGGCGA14950.011.11000817
TAGGACA5100.011.0162444
Produced by FastQC (version 0.11.5)