##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513095_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1730612
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.37097916806309	31.0	31.0	34.0	26.0	34.0
2	30.52045750289493	31.0	31.0	34.0	27.0	34.0
3	30.5389804300444	31.0	31.0	34.0	26.0	34.0
4	33.73074553972814	37.0	35.0	37.0	28.0	37.0
5	33.647277379331705	37.0	35.0	37.0	28.0	37.0
6	33.60428796287094	37.0	35.0	37.0	28.0	37.0
7	33.51377027317504	37.0	35.0	37.0	28.0	37.0
8	33.514449801573086	37.0	35.0	37.0	28.0	37.0
9	35.052774394260524	39.0	35.0	39.0	28.0	39.0
10	34.95049092459777	39.0	35.0	39.0	27.0	39.0
11	34.962728791895586	39.0	35.0	39.0	27.0	39.0
12	34.880138355679954	39.0	35.0	39.0	27.0	39.0
13	34.84780817421814	39.0	35.0	39.0	27.0	39.0
14	36.00247022440617	40.0	36.0	41.0	27.0	41.0
15	35.99436153222097	40.0	36.0	41.0	27.0	41.0
16	35.94111793978084	40.0	36.0	41.0	27.0	41.0
17	35.87056139677755	40.0	36.0	41.0	27.0	41.0
18	35.79654653960564	40.0	36.0	41.0	27.0	41.0
19	35.85498309268629	40.0	36.0	41.0	27.0	41.0
20	35.77301613533247	40.0	36.0	41.0	27.0	41.0
21	35.791491680399766	40.0	36.0	41.0	27.0	41.0
22	35.780522150545586	40.0	36.0	41.0	27.0	41.0
23	35.71111780110157	40.0	36.0	41.0	27.0	41.0
24	35.58543451680677	40.0	36.0	41.0	26.0	41.0
25	35.56777371242081	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6866195798646899
1101	2	0.6076809907410592
1101	3	0.6477270192369389
1101	4	0.9311671819091885
1101	5	0.7497420097380285
1101	6	0.7002604015979657
1101	7	0.6822215933010298
1101	8	0.6559985213757002
1101	9	0.7329933563336297
1101	10	0.7562708172008286
1101	11	0.7326427583550057
1101	12	0.751252049389656
1101	13	0.8190532924019891
1101	14	0.7836657618733653
1101	15	0.7327426194992199
1101	16	0.8192383638927723
1101	17	0.9253177040465559
1101	18	0.721477120723307
1101	19	0.7994294955752252
1101	20	0.9998395192835545
1101	21	0.9094264453196175
1101	22	0.8767155674749247
1101	23	0.7772598009258616
1101	24	0.8465538156603358
1101	25	0.8480545814699312
1102	1	0.6302149961959778
1102	2	0.5293262956843172
1102	3	0.585164171078091
1102	4	0.7996868384190634
1102	5	0.6404828370421214
1102	6	0.5379955762730901
1102	7	0.5502587013622389
1102	8	0.6057653133776526
1102	9	0.6767763195516352
1102	10	0.5909051976804491
1102	11	0.6302686655916929
1102	12	0.7101208509365478
1102	13	0.6634478066840543
1102	14	0.6880881060612296
1102	15	0.7515064289926272
1102	16	0.8470694098001488
1102	17	0.8281621461601247
1102	18	0.7860901584812225
1102	19	0.7727961275067656
1102	20	0.9115873080544503
1102	21	0.9459533392011465
1102	22	0.9927050511680306
1102	23	0.845041183961591
1102	24	0.8596014354680221
1102	25	0.8978274730210885
1103	1	-0.1496885936184249
1103	2	-0.04607484977600862
1103	3	0.2340422997011551
1103	4	-0.6489871987771068
1103	5	-0.22923824081432542
1103	6	-0.16415190223514742
1103	7	0.02789691001652983
1103	8	-0.053292548730972555
1103	9	-0.029147215204105237
1103	10	0.1294200171821771
1103	11	0.14579274863265823
1103	12	0.11305211124881964
1103	13	0.05012640674933522
1103	14	0.07018882203708188
1103	15	0.010740429411924879
1103	16	0.12080633449147626
1103	17	0.14104044991114506
1103	18	0.09177698984114357
1103	19	0.13114329005497893
1103	20	0.18521730365660716
1103	21	0.22357675740137495
1103	22	0.2915870107151619
1103	23	0.16093216568569346
1103	24	0.1548457010798785
1103	25	0.1424635478647147
1104	1	0.4933823861331028
1104	2	0.44873184508783126
1104	3	0.5820818428178463
1104	4	0.43361571005651456
1104	5	0.3825417904285189
1104	6	0.46498981564851505
1104	7	0.31286540741053415
1104	8	0.4546656354456786
1104	9	0.3768244947107817
1104	10	0.3706997973222741
1104	11	0.24267387931246276
1104	12	0.3917086799290672
1104	13	0.41243207699851325
1104	14	0.32947007168489506
1104	15	0.36152107446149273
1104	16	0.39063678584695083
1104	17	0.36401433110202674
1104	18	0.2885909673719098
1104	19	0.21140587178707193
1104	20	0.3892889340487571
1104	21	0.552014755363416
1104	22	0.41603048208490634
1104	23	0.43358370901239596
1104	24	0.5159215588493424
1104	25	0.5566437200659493
1105	1	0.6270004591301941
1105	2	0.5759619420775053
1105	3	0.6547813544608303
1105	4	0.7236146171236797
1105	5	0.6645612056769892
1105	6	0.6212056276813769
1105	7	0.5314993171126403
1105	8	0.6003433024993754
1105	9	0.5816952959820441
1105	10	0.6132334969864957
1105	11	0.6039060366428544
1105	12	0.5402682810194008
1105	13	0.5683286660741587
1105	14	0.699436764286169
1105	15	0.5769127069244107
1105	16	0.6593427100839833
1105	17	0.617042079692844
1105	18	0.5343041817955978
1105	19	0.46883538032439276
1105	20	0.6951791927708442
1105	21	0.6613104737719766
1105	22	0.619602478764989
1105	23	0.6631997000265386
1105	24	0.7638406580129811
1105	25	0.7921369303559658
1106	1	-0.45523977871270205
1106	2	-0.26957492494794266
1106	3	-0.14407154906689712
1106	4	-1.2792851943087484
1106	5	-0.5655988703006045
1106	6	-0.4500718144063569
1106	7	-0.3055734809824173
1106	8	-0.23978635491325662
1106	9	-0.11550151626245508
1106	10	-0.05752999522992752
1106	11	-0.04455307722719226
1106	12	-0.06449504602834821
1106	13	-0.07912484582486456
1106	14	-0.06442474177689661
1106	15	0.024584792041338233
1106	16	0.032184987760054184
1106	17	-0.019319116678950365
1106	18	-0.12942876797936265
1106	19	-0.09723016411286522
1106	20	0.083962037250771
1106	21	0.09499401058394596
1106	22	0.06539948645909988
1106	23	0.04329962707704027
1106	24	0.13600684523167672
1106	25	0.11989476554515477
1107	1	0.49874781656200895
1107	2	0.2817066149956098
1107	3	0.5411276194447048
1107	4	0.7867566167135465
1107	5	0.6538809424455039
1107	6	0.5586527297115396
1107	7	0.5141422581465491
1107	8	0.44433390670138806
1107	9	0.4826441970092432
1107	10	0.4631635877752558
1107	11	0.46584559779942936
1107	12	0.5842355821509955
1107	13	0.4905001393874855
1107	14	0.5710210002251017
1107	15	0.5704325891040867
1107	16	0.5038599636773142
1107	17	0.5225071157659968
1107	18	0.5607214745380915
1107	19	0.5498219391133787
1107	20	0.6610001133353407
1107	21	0.5537369758878938
1107	22	0.6704749457212387
1107	23	0.5697264239876887
1107	24	0.6296662391915575
1107	25	0.5735345553070275
1108	1	-0.4077624597767908
1108	2	-0.27011277797806343
1108	3	0.005855495645977982
1108	4	-1.0206366611029836
1108	5	-0.6009768880354045
1108	6	-0.4335883617589502
1108	7	-0.30703258672486555
1108	8	-0.2840051182613408
1108	9	-0.12857332681790723
1108	10	-0.06357076134587203
1108	11	-0.12822100252917323
1108	12	-0.06833656049126091
1108	13	-0.05962135183043671
1108	14	-0.07746374808146328
1108	15	-0.024709745709181163
1108	16	-0.0012869663139909449
1108	17	-0.015016975859865056
1108	18	-0.16799904937832366
1108	19	-0.18325111099390057
1108	20	-0.019021441024953845
1108	21	-0.05968623339767021
1108	22	-0.1254515446536857
1108	23	0.006753641326383786
1108	24	-0.08874191179475588
1108	25	-0.06540346268359087
1109	1	-0.25398123434986175
1109	2	-0.4200751200053574
1109	3	0.04324870128505154
1109	4	-0.6721932459395958
1109	5	-0.35894859976445304
1109	6	-0.13194368712714777
1109	7	-0.14283453564937787
1109	8	-0.066616756731527
1109	9	0.009361689124517625
1109	10	0.14403249946959562
1109	11	0.1529875031958312
1109	12	0.15752235648535162
1109	13	0.1217666513680058
1109	14	0.0793566582264944
1109	15	0.16877326838850593
1109	16	0.13585308484776704
1109	17	-0.010478775413282904
1109	18	-0.005769830109386476
1109	19	0.03319099028779959
1109	20	0.2149191755040718
1109	21	0.11747412320840311
1109	22	0.08682594981375047
1109	23	0.181495261183251
1109	24	0.24081611300310612
1109	25	0.19428525352792292
1110	1	0.5482290800089018
1110	2	0.26272059316379526
1110	3	0.4516600833773339
1110	4	0.8168598360480317
1110	5	0.6406096023100787
1110	6	0.4949728680412875
1110	7	0.43059201461755947
1110	8	0.33386941969619954
1110	9	0.2971576010033843
1110	10	0.3272090729193309
1110	11	0.2965718289737822
1110	12	0.37292633332904046
1110	13	0.34372937342610754
1110	14	0.37126254930708313
1110	15	0.45494770902066506
1110	16	0.5120178549406305
1110	17	0.6301931283582292
1110	18	0.46119269323899914
1110	19	0.326173888005286
1110	20	0.44753936176130793
1110	21	0.4040069036871543
1110	22	0.5497149240742161
1110	23	0.5580687109163165
1110	24	0.6221095807525217
1110	25	0.6676661005611848
1111	1	0.36670842082255106
1111	2	-0.10029290584927963
1111	3	0.19896557968795747
1111	4	0.6370626524270122
1111	5	0.3840641044097808
1111	6	0.30217609508700605
1111	7	0.24772807778074935
1111	8	0.14753932312328288
1111	9	0.15273736970527807
1111	10	0.22570219093964994
1111	11	0.12140855582740784
1111	12	0.1477219935089451
1111	13	0.1860134753244438
1111	14	0.08806809198330257
1111	15	0.11142299615620033
1111	16	0.16924691424885197
1111	17	0.13616369282628682
1111	18	0.15176193032618102
1111	19	0.07602220625876299
1111	20	0.21891191597594428
1111	21	0.2169296585986018
1111	22	0.23310544164677793
1111	23	0.14374571490375843
1111	24	0.22774896236789743
1111	25	0.3035411518945281
1112	1	0.15085241882279732
1112	2	-0.2726190794686545
1112	3	0.1032545078660192
1112	4	0.42070059257495984
1112	5	0.15886458455934616
1112	6	0.049959829890298124
1112	7	-0.010630534576591799
1112	8	-0.07017869055733428
1112	9	-0.10298901274270378
1112	10	-0.03279260266634054
1112	11	0.03924061804896439
1112	12	-0.10268394638904255
1112	13	-0.07127183090109668
1112	14	-0.08305679637671659
1112	15	-0.07464837010886782
1112	16	0.0420506335852906
1112	17	-0.07409794181739215
1112	18	-0.1916558023992394
1112	19	-0.2398007268718203
1112	20	-0.17806306364894908
1112	21	-0.10484280675069613
1112	22	-0.06241893427596068
1112	23	-0.13779625782353833
1112	24	-0.10632772616241226
1112	25	-0.0884764917792964
1113	1	0.11988498967119199
1113	2	-0.014328431311469103
1113	3	0.0758723220666333
1113	4	0.600236831199453
1113	5	0.21331128293796553
1113	6	0.16832736327194198
1113	7	0.0709450415435171
1113	8	-0.06437336818130746
1113	9	-0.12860375194959772
1113	10	-0.2085515405996361
1113	11	-0.1577406558414154
1113	12	-0.13919404652877176
1113	13	-0.08681030777219689
1113	14	-0.08362933487642721
1113	15	-0.1077356970236778
1113	16	-0.1960625743536326
1113	17	-0.08011141282296563
1113	18	-0.30131483314294627
1113	19	-0.28904630488136007
1113	20	-0.2028187355693305
1113	21	-0.25982685041934417
1113	22	-0.1419690840806851
1113	23	-0.12591673279690951
1113	24	-0.2494322448044315
1113	25	-0.21513519239201173
1114	1	0.17613344636545492
1114	2	-0.09360134115571839
1114	3	0.05412679130036935
1114	4	0.42357715828529763
1114	5	0.11208069393805431
1114	6	0.03733413918625672
1114	7	-0.031141969841392836
1114	8	-0.11131950944210445
1114	9	-0.04469745967713834
1114	10	-0.04108855811927015
1114	11	-0.1886454013429173
1114	12	-0.27485889878981595
1114	13	-0.1760312188012989
1114	14	-0.23902142052225628
1114	15	-0.22073955907409015
1114	16	-0.2167365824925369
1114	17	-0.11976634891149729
1114	18	-0.2647162199491362
1114	19	-0.3937330003410011
1114	20	-0.2147905528172913
1114	21	-0.21274672070208567
1114	22	-0.2915165008499798
1114	23	-0.15715721948815542
1114	24	-0.14623893026370638
1114	25	-0.15342824619428086
1115	1	-0.08078647660834548
1115	2	-0.19881146154112983
1115	3	-0.09596056407839626
1115	4	0.3575452827291201
1115	5	0.019851392866762296
1115	6	0.008095075246536965
1115	7	-0.13593341019382876
1115	8	-0.1628920242154095
1115	9	-0.3531911390613587
1115	10	-0.26903922489702126
1115	11	-0.3673706199734852
1115	12	-0.4127985733542445
1115	13	-0.4146034702233763
1115	14	-0.4595269828702584
1115	15	-0.5142516451076276
1115	16	-0.6050296580189638
1115	17	-0.600817911244846
1115	18	-0.4415576267587511
1115	19	-0.7084461641652027
1115	20	-0.4979892285368166
1115	21	-0.560187327922236
1115	22	-0.522816111236672
1115	23	-0.6004846182099897
1115	24	-0.3951166455223003
1115	25	-0.4148263817271669
1116	1	-0.5675159803855259
1116	2	-0.7345198437412641
1116	3	-0.5249966283339589
1116	4	-0.1295659639810296
1116	5	-0.42691266494646385
1116	6	-0.5502682304663935
1116	7	-0.810225002791249
1116	8	-0.6530954628074994
1116	9	-0.9314556304307757
1116	10	-0.884878932596088
1116	11	-0.8892292203156131
1116	12	-0.9342038261666517
1116	13	-0.9771723229353881
1116	14	-0.9750292298165704
1116	15	-1.0914419192761429
1116	16	-1.1321847747752187
1116	17	-0.9682153154225475
1116	18	-1.2131096096946195
1116	19	-1.3249166742641947
1116	20	-1.2672085027152633
1116	21	-1.2687653658880862
1116	22	-1.1001733145479164
1116	23	-1.2905467155545836
1116	24	-0.993627596059234
1116	25	-1.2010549350015012
1201	1	0.7540996362979833
1201	2	0.7197683885738648
1201	3	0.7911836793681317
1201	4	1.0331699816735878
1201	5	0.8358980162066487
1201	6	0.8195582877700787
1201	7	0.8005929003841175
1201	8	0.7357392175476605
1201	9	0.7842618086713529
1201	10	0.9025118251621151
1201	11	0.8356492200590964
1201	12	0.6913212135983144
1201	13	0.8402090049472122
1201	14	0.7613693672183857
1201	15	0.8925735501528962
1201	16	0.8815639549381373
1201	17	0.882911186093601
1201	18	1.0652533793205663
1201	19	0.9431197095399355
1201	20	1.0875461157313424
1201	21	0.9946269126001823
1201	22	0.9635359272602102
1201	23	0.959948750443651
1201	24	1.046802814879996
1201	25	1.0183402519552942
1202	1	0.7605957015641209
1202	2	0.6376554652275495
1202	3	0.6692366653204935
1202	4	0.8735137287633066
1202	5	0.777663898008754
1202	6	0.70717203736843
1202	7	0.7361455265848988
1202	8	0.578184142041188
1202	9	0.7354200233508479
1202	10	0.5892315938714461
1202	11	0.636287517051457
1202	12	0.6115743075688513
1202	13	0.6906791350125658
1202	14	0.797394155777873
1202	15	0.8880493086012109
1202	16	0.7486494023913721
1202	17	0.8840765102344079
1202	18	0.8582598086410371
1202	19	0.7517874906164437
1202	20	0.8734477900986164
1202	21	0.8451307002958401
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2210	23	0.22347817863067831
2210	24	0.24243031663273484
2210	25	0.2286406887754069
2211	1	0.3696636164973235
2211	2	0.21772305091947075
2211	3	0.3948941198578275
2211	4	0.6467775937291762
2211	5	0.30225037885516315
2211	6	0.2579366042527482
2211	7	0.20024243190746915
2211	8	0.11841040078998333
2211	9	0.055248109838089476
2211	10	0.1978262074404924
2211	11	0.17628107383495717
2211	12	0.14010868822896327
2211	13	0.1602835471180768
2211	14	0.17552477333811822
2211	15	0.1923111450159496
2211	16	0.2653524744113369
2211	17	0.22782469756916157
2211	18	0.1812844494507786
2211	19	0.22292277937248883
2211	20	0.3145895792594757
2211	21	0.1641384068293874
2211	22	0.0336951140349413
2211	23	-0.010748976467731097
2211	24	-3.0504512949484734
2211	25	-2.0599374026656108
2212	1	0.00477107092369522
2212	2	-0.18536073389820729
2212	3	-0.5321723820168316
2212	4	0.23890593783021075
2212	5	-0.07006658576545277
2212	6	-0.035744938208942756
2212	7	-0.10153576819563881
2212	8	-0.1243204042310353
2212	9	-0.2227346529747436
2212	10	-0.22959410422070903
2212	11	-0.2518154667566037
2212	12	-0.2875712612906298
2212	13	-0.2892578568736397
2212	14	-0.2623741604973233
2212	15	-0.17595137226885527
2212	16	-0.14463139931102376
2212	17	-0.2553831707348593
2212	18	-0.305457526382682
2212	19	-0.3363531289476853
2212	20	-0.6878602209843123
2212	21	-0.5831799544088128
2212	22	-0.4785323393699059
2212	23	-0.3617873416856199
2212	24	-0.2959814536414669
2212	25	-0.3636354863998861
2213	1	-0.39322604250506643
2213	2	-0.6664275772340211
2213	3	-0.5190862178119211
2213	4	0.01608179659050535
2213	5	-0.40546218890846575
2213	6	-0.5987860699760503
2213	7	-0.5859833890219761
2213	8	-0.6154881063137623
2213	9	-0.710472076669781
2213	10	-0.8233592387855495
2213	11	-0.7979037453187559
2213	12	-0.865374898456011
2213	13	-0.7374807211507459
2213	14	-0.8858679284681585
2213	15	-0.9818928713058597
2213	16	-0.8618108328388061
2213	17	-1.0878769510140245
2213	18	-0.9508221285854432
2213	19	-0.9796772238973617
2213	20	-6.336928025440248
2213	21	-3.3479811240213593
2213	22	-2.1832434334039448
2213	23	-1.6525590901054699
2213	24	-1.247149487514001
2213	25	-1.1073760310595944
2214	1	0.06612539159813124
2214	2	-0.20678648340780015
2214	3	0.0900430828376173
2214	4	0.4473289176395099
2214	5	0.12854903880433
2214	6	0.1365585335953341
2214	7	-0.004544303050032283
2214	8	-0.08854295500315601
2214	9	-0.16083177234930446
2214	10	-0.2841325675038533
2214	11	-0.19121093259212785
2214	12	-0.14629228321562238
2214	13	-0.1820025185677565
2214	14	-0.25147575116294263
2214	15	-0.2168610218076168
2214	16	-0.23831383479060975
2214	17	-0.30578079758011967
2214	18	-0.3900913599780722
2214	19	-0.3050709702798855
2214	20	-0.16042800374191302
2214	21	-0.2770244260585457
2214	22	-0.30780887846297844
2214	23	-0.465840197546747
2214	24	-0.3683982241740935
2214	25	-0.4119116631269151
2215	1	-0.4268995378091347
2215	2	-0.46897171136411586
2215	3	-0.37734455226336294
2215	4	-0.09559250272654651
2215	5	-0.45765273947264973
2215	6	-0.5873179817747527
2215	7	-0.6613470355774567
2215	8	-0.8624814594814723
2215	9	-0.8839600691950764
2215	10	-0.9024843525954651
2215	11	-0.8529856897371886
2215	12	-0.8252739170932557
2215	13	-0.9037338795441272
2215	14	-0.9310709545739471
2215	15	-1.0291346629464329
2215	16	-1.097282608109225
2215	17	-1.1561884236943811
2215	18	-1.0181942606334147
2215	19	-0.9944700109722007
2215	20	-0.9666673471908993
2215	21	-0.9615533455010237
2215	22	-1.060750105505953
2215	23	-0.9230285192690815
2215	24	-1.0158823615115153
2215	25	-1.0431066861967722
2216	1	-2.133667819427714
2216	2	-1.5959637166583391
2216	3	-3.32032490172487
2216	4	-1.729151587765024
2216	5	-1.8237013908330297
2216	6	-1.708594146023792
2216	7	-1.7133499815571547
2216	8	-1.6251219538937143
2216	9	-1.8426416365416642
2216	10	-2.0753124931058835
2216	11	-1.945760034096601
2216	12	-1.8611727077472437
2216	13	-1.9958838042935554
2216	14	-2.1831176632955476
2216	15	-2.1836545002771217
2216	16	-2.272990016821211
2216	17	-2.3064574044494535
2216	18	-2.2192306904475814
2216	19	-2.181988023076336
2216	20	-2.0944145266288174
2216	21	-2.067344437114592
2216	22	-2.2983978047598512
2216	23	-2.3628734283813344
2216	24	-2.442302249009245
2216	25	-2.4167230897532974
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51967.0
3	1.0
4	865.0
5	6694.0
6	7744.0
7	5845.0
8	4695.0
9	4105.0
10	3539.0
11	3183.0
12	2794.0
13	2735.0
14	2707.0
15	2643.0
16	2653.0
17	2757.0
18	3191.0
19	3707.0
20	4706.0
21	6333.0
22	7984.0
23	9420.0
24	10900.0
25	12063.0
26	13206.0
27	14314.0
28	16191.0
29	19278.0
30	24556.0
31	31149.0
32	39404.0
33	51689.0
34	68833.0
35	94643.0
36	139175.0
37	234439.0
38	820504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.147395112688365	19.057099685642	13.244371370844018	27.55113383082561
2	12.800383927100107	20.157097881173407	40.44070668027372	26.601811511452766
3	17.747431398506077	24.640488243747438	28.968843951644825	28.643236406101657
4	10.15328681414436	17.212985926365935	37.09265855084791	35.5410687086418
5	12.127457297990816	38.59319599723749	36.08311829417357	13.196228410598131
6	32.92187920521935	36.22874769147959	17.71760415023248	13.131768953068592
7	27.988695171814438	31.15455556146235	21.64801789911868	19.208731367604535
8	25.499967632153293	37.861537287759404	19.729878759294195	16.908616320793104
9	26.398381107838286	14.981977628676727	19.568896554594257	39.050744708890726
10	14.135556884601272	30.19198932140659	34.371722529925194	21.30073126406694
11	33.884351485131354	22.97674617492433	23.15431936824855	19.98458297169577
12	23.378094355857932	25.56966428944164	31.479691109070533	19.57255024562989
13	29.209039352132766	21.351210474793668	25.067524695488764	24.372225477584806
14	20.44642939691756	22.84932877839517	27.39012906685404	29.314112757833232
15	24.722843390480815	29.920143764337432	22.677899700105744	22.679113145076013
16	21.21132791844731	28.135042749860585	29.15497149527714	21.498657836414964
17	22.195288857447366	28.909036895241925	27.924928736397497	20.970745510913215
18	22.238855160451	27.148540040474124	29.800057820179244	20.812546978895636
19	23.050450496274184	27.599803941062095	28.21838376225079	21.131361800412925
20	23.15926389046187	27.536790453319405	28.32668443302138	20.977261223197345
21	21.84325544951728	27.251457865772338	28.19372568779137	22.711560996919008
22	22.22473899406684	28.3320004715095	26.471560349749108	22.971700184674553
23	20.867533761275666	28.259564418727074	27.856064375100758	23.016837444896503
24	22.229502892043847	28.73096769347224	27.791645720295154	21.247883694188754
25	22.039139911199044	27.019112314025328	27.171255024234203	23.77049275054143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	111.0
2	262.0
3	413.0
4	413.0
5	413.0
6	988.5
7	1564.0
8	1564.0
9	1564.0
10	2225.0
11	2886.0
12	2886.0
13	2886.0
14	4730.5
15	6575.0
16	6575.0
17	6575.0
18	12358.5
19	18142.0
20	18142.0
21	18142.0
22	29103.0
23	40064.0
24	40064.0
25	40064.0
26	57564.5
27	75065.0
28	75065.0
29	75065.0
30	97627.5
31	120190.0
32	120190.0
33	120190.0
34	137911.0
35	155632.0
36	155632.0
37	155632.0
38	169788.5
39	183945.0
40	183945.0
41	183945.0
42	196895.5
43	209846.0
44	209846.0
45	209846.0
46	246653.0
47	283460.0
48	283460.0
49	283460.0
50	262453.5
51	241447.0
52	241447.0
53	241447.0
54	201238.5
55	161030.0
56	161030.0
57	161030.0
58	133879.0
59	106728.0
60	106728.0
61	106728.0
62	85904.5
63	65081.0
64	65081.0
65	65081.0
66	49604.0
67	34127.0
68	34127.0
69	34127.0
70	24692.5
71	15258.0
72	15258.0
73	15258.0
74	10502.0
75	5746.0
76	5746.0
77	5746.0
78	3967.5
79	2189.0
80	2189.0
81	2189.0
82	1497.0
83	805.0
84	805.0
85	805.0
86	516.5
87	228.0
88	228.0
89	228.0
90	149.0
91	70.0
92	70.0
93	70.0
94	39.0
95	8.0
96	8.0
97	8.0
98	5.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1896438947609285
2	0.06454364120900584
3	5.778302704476798E-5
4	0.0
5	0.01773938930274377
6	0.379287789521857
7	0.0013867926490744313
8	0.028891513522383987
9	0.031202834604174706
10	0.002715802271104095
11	0.0033514155685965426
12	0.00941863340829718
13	0.007742925623998908
14	0.002080188973611647
15	1.1556605408953596E-4
16	0.011383256327819292
17	0.02403773925062348
18	0.06425472607378199
19	0.03004717406327935
20	0.0
21	0.0
22	0.0
23	2.889151352238399E-4
24	1.1556605408953596E-4
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1730612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.31944121653723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.58140411347715	59.32455111672896
2	10.161039139166544	14.087151106127784
3	2.292705766773055	4.767872478799221
4	0.7725654670805523	2.142152259248679
5	0.3385016657502663	1.1732373160337757
6	0.20155236279511185	0.8382898298897964
7	0.1268496180007446	0.6155201246839971
8	0.08538999906913199	0.47353496167622905
9	0.0637860150219346	0.397944982687514
>10	0.329384719624624	4.106348756354835
>50	0.023327219017651103	1.122267455451904
>100	0.017976315377198473	2.4589841027065167
>500	0.0020899995628949164	1.0325716438531678
>1k	0.0030931993530844758	4.653990130553601
>5k	1.6719996503159333E-4	0.7097843469476709
>10k+	1.6719996503159333E-4	2.095799388256247
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	18893	1.0916947299568014	No Hit
CCCATGTACTCTGCGTTGATACCAC	17272	0.9980284431172326	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7219	0.41713567223618	No Hit
CATGTACTCTGCGTTGATACCACTG	5029	0.29059084300813814	No Hit
GTATCAACGCAGAGTACATGGGAAG	4762	0.27516277478718515	No Hit
GCGTTGATACCACTGCTTCCCATGT	4674	0.2700778684072455	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4489	0.2593880084039634	No Hit
TATCAACGCAGAGTACATGGGAAGC	4150	0.2397995622357871	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3794	0.21922880460784971	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3631	0.20981017119955253	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3614	0.20882785973979148	No Hit
GTCCTACAGTGGACATTTCTAAATT	2947	0.17028658070093122	No Hit
GGTATCAACGCAGAGTACATGGGAA	2914	0.1683797408084539	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2653	0.15329837074976946	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2445	0.1412795011244577	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2344	0.13544341539293614	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2190	0.1265448292280419	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1934	0.11175237430458126	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1925	0.11123232706117836	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1925	0.11123232706117836	No Hit
CAGTGGTATCAACGCAGAGTACATG	1913	0.11053893073664114	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1853	0.10707194911395507	No Hit
ATACCACTGCTTCCCATGTACTCTG	1774	0.10250708997741839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.778302704476798E-5
2	0.0	0.0	0.0	0.0	5.778302704476798E-5
3	0.0	0.0	0.0	0.0	5.778302704476798E-5
4	0.0	0.0	0.0	0.0	5.778302704476798E-5
5	0.0	0.0	0.0	0.0	5.778302704476798E-5
6	0.0	0.0	0.0	0.0	5.778302704476798E-5
7	0.0	0.0	0.0	0.0	5.778302704476798E-5
8	0.0	0.0	0.0	5.778302704476798E-5	5.778302704476798E-5
9	0.0	0.0	0.0	5.778302704476798E-5	5.778302704476798E-5
10	0.0	0.0	0.0	5.778302704476798E-5	5.778302704476798E-5
11	0.0	0.0	0.0	5.778302704476798E-5	5.778302704476798E-5
12	0.0	0.0	0.0	1.1556605408953596E-4	5.778302704476798E-5
13	0.0	0.0	0.0	1.7334908113430394E-4	5.778302704476798E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	650	0.0	15.382525	4
TGGTCGG	40	0.005341894	14.224752	10
AAGCCGC	50	0.00150994	13.287954	16
CGTGCGC	65	5.5446602E-5	13.130541	10
TCCAACG	125	1.05501385E-10	12.90494	18
GTAGGAC	1545	0.0	12.388515	3
TGTAGGA	1690	0.0	12.348785	2
CCAACGA	85	4.010026E-6	12.275883	19
TCCGTGT	55	0.0031029861	12.072976	8
AGGACCT	1110	0.0	11.838803	5
CTGTAGG	1660	0.0	11.829453	1
TGGCGAG	1350	0.0	11.808441	18
GGCGAGA	815	0.0	11.6391535	19
GGCGAGG	595	0.0	11.638174	19
TTAGGAC	845	0.0	11.269249	3
AGGACGT	1280	0.0	11.233592	5
GGACCTG	1095	0.0	11.218307	6
GGACGTG	1265	0.0	11.216244	6
ATGGCGA	1495	0.0	11.110008	17
TAGGACA	510	0.0	11.016244	4
>>END_MODULE