FastQCFastQC Report
Thu 26 May 2016
SRR1513094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873779
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC239361.2774185216079377No Hit
CCCATGTACTCTGCGTTGATACCAC192521.0274424038267054No Hit
GAGTACATGGGAAGCAGTGGTATCA102970.5495311880429868No Hit
GCGTTGATACCACTGCTTCCCATGT65910.3517490589872124No Hit
GTATCAACGCAGAGTACATGGGAAG63620.33952776714863386No Hit
CATGTACTCTGCGTTGATACCACTG62010.3309355052009869No Hit
ACGCAGAGTACATGGGAAGCAGTGG50330.2686015800155728No Hit
TATCAACGCAGAGTACATGGGAAGC50000.26684043315673833No Hit
GCTTCCCATGTACTCTGCGTTGATA44720.23866208341538678No Hit
ACTCTGCGTTGATACCACTGCTTCC42870.22878898738858747No Hit
GGTATCAACGCAGAGTACATGGGAA32830.1752074284107144No Hit
CAGTGGTATCAACGCAGAGTACATG24940.13310000805858108No Hit
GCAGTGGTATCAACGCAGAGTACAT21660.11559527564349904No Hit
CCACTGCTTCCCATGTACTCTGCGT20890.11148593297288527No Hit
GTGGTATCAACGCAGAGTACATGGG20390.1088175286413179No Hit
ATACCACTGCTTCCCATGTACTCTG20260.10812374351511038No Hit
CTGCTTCCCATGTACTCTGCGTTGA20050.10700301369585208No Hit
GTACTCTGCGTTGATACCACTGCTT19490.1040144008444966No Hit
GTATCAACGCAGAGTACTTTTTTTT19220.10257346250545023No Hit
GGGAAGCAGTGGTATCAACGCAGAG19050.1016662050327173No Hit
CCCATGTACTCTGCGTTTATACCAC19040.10161283694608596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC1500.014.56670819
CTAGACG602.563578E-514.2530854
GTATCGA1450.013.75865918
GCGTTTA3100.013.48174913
GGATCGT500.00150122513.2982626
CGTTTAT3250.013.15177614
TGCGTTT3400.012.57121512
ACCGCGC550.00307431312.0880388
GTAGGAC5250.011.9454423
GTCCTAC5400.011.8031441
GGTATCG1552.5465852E-1111.6436417
GTCTAAT1156.9430826E-811.5810211
TAGGACC2900.011.4679994
ATACGAC1251.832268E-811.40003119
TAGGACA2850.011.3357874
GTCCTAT2350.011.3346171
GTATAGG2050.011.137151
GTCTAAC600.005816989611.0984781
TGTTATC5000.011.01855916
GTACCGA700.001493276710.8557246
Produced by FastQC (version 0.11.5)