##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513094_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1873779
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.544975688168137	31.0	28.0	34.0	16.0	34.0
2	28.277985290687962	31.0	28.0	34.0	16.0	34.0
3	28.616633551768913	31.0	28.0	34.0	16.0	34.0
4	31.410784302737945	35.0	32.0	37.0	16.0	37.0
5	31.616655432684432	35.0	32.0	37.0	17.0	37.0
6	32.11554564332293	35.0	32.0	37.0	21.0	37.0
7	32.09701090683586	35.0	33.0	37.0	19.0	37.0
8	32.07975807178968	35.0	33.0	37.0	19.0	37.0
9	33.34900006884483	38.0	33.0	39.0	17.0	39.0
10	32.814521883317084	38.0	33.0	39.0	13.0	39.0
11	33.201675864656394	38.0	33.0	39.0	17.0	39.0
12	33.053075629516606	38.0	33.0	39.0	17.0	39.0
13	33.11139093777868	38.0	33.0	39.0	17.0	39.0
14	33.355483757689676	39.0	32.0	41.0	10.0	41.0
15	33.84981046324033	39.0	33.0	41.0	15.0	41.0
16	33.37883176191002	39.0	32.0	41.0	10.0	41.0
17	32.90342511043191	38.0	32.0	41.0	8.0	41.0
18	32.263162838306975	38.0	31.0	41.0	8.0	41.0
19	33.151537080947115	39.0	32.0	41.0	8.0	41.0
20	33.45153670737051	39.0	33.0	41.0	2.0	41.0
21	33.47001007055795	39.0	33.0	41.0	2.0	41.0
22	33.043901655424676	39.0	32.0	41.0	2.0	41.0
23	31.313434508551968	38.0	29.0	40.0	2.0	41.0
24	32.364596358481975	38.0	32.0	41.0	2.0	41.0
25	32.0863815850215	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8879042607782033
1101	2	1.2679023104739997
1101	3	1.0203132426860257
1101	4	1.1745290134613313
1101	5	1.3005927309784795
1101	6	0.9921231208584231
1101	7	0.9322311512769588
1101	8	0.8911476523160999
1101	9	1.0489721911709324
1101	10	1.4114483967231237
1101	11	1.0181034928242028
1101	12	1.0306161266610445
1101	13	0.9400253019362026
1101	14	0.09825335297431792
1101	15	0.6739578504338866
1101	16	1.4058258102510948
1101	17	2.032900107303007
1101	18	-4.570972472141175
1101	19	-0.8139225434256758
1101	20	0.3546022910048734
1101	21	0.8678359301614336
1101	22	1.468973862004411
1101	23	3.285580822408871
1101	24	2.409753491580389
1101	25	2.5337446201178437
1102	1	1.2767571977312109
1102	2	1.4498945788658553
1102	3	1.2946006385776165
1102	4	1.9243188127267175
1102	5	1.741703932363066
1102	6	1.2969600050883656
1102	7	1.1372108775342014
1102	8	1.0730204133240022
1102	9	1.3559210023322592
1102	10	1.74947196187874
1102	11	1.4362248745374018
1102	12	1.4296304977782341
1102	13	1.3950673798720246
1102	14	2.3179386281530583
1102	15	1.7833467850893285
1102	16	2.0922521332403505
1102	17	1.711321325012392
1102	18	2.150777966210022
1102	19	1.98126022257037
1102	20	1.755307242861818
1102	21	1.7789011802483543
1102	22	2.259929467609254
1102	23	3.937749149267706
1102	24	2.783276418103661
1102	25	3.146748062686285
1103	1	1.0394604417633637
1103	2	1.2481981809233567
1103	3	0.9794014034846441
1103	4	1.5360684993829707
1103	5	1.4914347215408128
1103	6	0.9714418965156497
1103	7	0.976139430389459
1103	8	0.9768824495168147
1103	9	1.0356706947138647
1103	10	1.5009561401070357
1103	11	1.1310077906595666
1103	12	1.326287297754952
1103	13	1.2400260404450094
1103	14	2.0009153913348428
1103	15	1.4693955574545754
1103	16	1.8285369332418782
1103	17	2.1834046279297965
1103	18	2.0553419544138833
1103	19	1.5267254813729778
1103	20	1.4977040946816018
1103	21	1.4623515970301213
1103	22	1.7479253129121517
1103	23	3.36749717175498
1103	24	2.257909530390691
1103	25	2.44146282326399
1104	1	0.7631028797268229
1104	2	1.1811448112165017
1104	3	1.1211466997694828
1104	4	1.6452503529326705
1104	5	1.4428168919526385
1104	6	1.0257082856935895
1104	7	0.9200782703740842
1104	8	0.9169476968661385
1104	9	1.1242135039122445
1104	10	1.4950057177804439
1104	11	1.0560211494918619
1104	12	1.24480345959838
1104	13	1.03761031827122
1104	14	1.984355595326555
1104	15	1.4569861397121713
1104	16	1.774902510577796
1104	17	2.250742400145299
1104	18	2.8792966843779766
1104	19	2.0145579288047983
1104	20	1.442777966680552
1104	21	1.4423180358935426
1104	22	1.7638431818737317
1104	23	3.3264369257649697
1104	24	2.4633156388925315
1104	25	2.5305797107029377
1105	1	0.4598218133343437
1105	2	0.9493329722035746
1105	3	0.5328737121079072
1105	4	0.7172905997011796
1105	5	0.8824504113415941
1105	6	0.5594718726501142
1105	7	0.34947016226597327
1105	8	0.4454656169601492
1105	9	0.7141022377547799
1105	10	1.0310882677216924
1105	11	0.7126769221123084
1105	12	0.7314753896806323
1105	13	0.6108476860738037
1105	14	1.352071884985783
1105	15	0.7657295298414368
1105	16	1.1266063883686357
1105	17	1.651517223882081
1105	18	1.6369751508879702
1105	19	1.2244158165838854
1105	20	0.9537870736135687
1105	21	0.8790377039311466
1105	22	1.2961507611921803
1105	23	2.913962630817853
1105	24	1.8823630830758518
1105	25	1.9699157859085759
1106	1	-0.47582390888122106
1106	2	0.04101870992568735
1106	3	-0.13283308118307602
1106	4	-2.538781861569909
1106	5	-0.4236049140711664
1106	6	-0.3999636646854583
1106	7	-0.34750606102830517
1106	8	-0.28917110284328373
1106	9	-0.26149718955172574
1106	10	-0.19315831634327196
1106	11	-0.06899421735672462
1106	12	-0.04593987677728251
1106	13	-0.0979981623665509
1106	14	0.6060452036116146
1106	15	-0.007207706097801747
1106	16	-1.1493708237489457
1106	17	-5.925879268042532
1106	18	-7.246670030096659
1106	19	-3.0694433191695687
1106	20	-1.4554855794293076
1106	21	-1.068261806526408
1106	22	-1.571602549867663
1106	23	-5.080971990732504
1106	24	-2.206713884083232
1106	25	-0.8254783201052653
1107	1	0.5159921664408671
1107	2	0.8752432029593962
1107	3	0.5889026573642298
1107	4	0.8743713424487467
1107	5	0.9768488900382621
1107	6	0.48947373841661346
1107	7	0.4375298014240059
1107	8	0.5030115756463616
1107	9	0.532585741836769
1107	10	0.9521454627710071
1107	11	0.6010758266213827
1107	12	0.6381138640556259
1107	13	0.5713942149309972
1107	14	1.4369246528571935
1107	15	0.8394820084509433
1107	16	-5.740294096821447
1107	17	-1.0913699385549123
1107	18	0.6479656958552127
1107	19	0.7162553579412503
1107	20	0.5999038279243507
1107	21	0.6767569519228331
1107	22	0.7982412367946665
1107	23	2.6969619571444987
1107	24	1.6072494574421228
1107	25	1.2788385985583801
1108	1	0.2424253606786415
1108	2	0.592648821120644
1108	3	0.3963841762152782
1108	4	0.8649838831958405
1108	5	0.7251609107777952
1108	6	0.279547962352396
1108	7	0.22689101103660647
1108	8	0.2618531614415289
1108	9	0.4932625967909061
1108	10	0.8443017087866096
1108	11	0.4250479398389615
1108	12	0.5224461958003914
1108	13	0.37258216701454216
1108	14	1.0452765533340767
1108	15	0.6699268422874312
1108	16	0.8401234194287142
1108	17	1.1211968938778298
1108	18	0.8511843433404138
1108	19	0.7540031419532767
1108	20	0.5367132711328608
1108	21	0.6513883389348791
1108	22	0.9280815276631031
1108	23	2.71242376727135
1108	24	1.5456017394086814
1108	25	1.714192698058767
1109	1	0.24400960374257608
1109	2	0.6411527946396838
1109	3	0.5213585071626703
1109	4	0.48906084530356964
1109	5	0.3660682755964899
1109	6	0.2869263407881846
1109	7	0.1964042810844262
1109	8	0.21570084762726083
1109	9	0.275463941206489
1109	10	0.8971582146184716
1109	11	0.518054141660933
1109	12	0.5561958717582982
1109	13	0.3158551537140326
1109	14	-0.9261677225482359
1109	15	0.04531486837445442
1109	16	-0.38407648584311715
1109	17	0.8396758282630898
1109	18	1.60626966632109
1109	19	0.7572241678948686
1109	20	0.7040671101220326
1109	21	0.5725195949035964
1109	22	0.3866647837610486
1109	23	-4.240892372501101
1109	24	-1.5298143563532918
1109	25	-0.1884604172112354
1110	1	0.2604497740207208
1110	2	0.541320424617922
1110	3	0.3930011826262678
1110	4	0.9223087394956693
1110	5	0.6300304303758537
1110	6	0.2598425845744359
1110	7	0.12098925377354419
1110	8	0.18495652201782065
1110	9	0.2423148508154327
1110	10	0.6643665615076486
1110	11	0.26390507027900867
1110	12	0.3204402138641598
1110	13	0.24770653199646375
1110	14	1.0781379556987787
1110	15	0.49974942636946906
1110	16	0.9955150668470338
1110	17	1.277389776619863
1110	18	2.009414738574378
1110	19	1.0748754385462718
1110	20	0.6217599605242938
1110	21	0.4601996436368978
1110	22	0.9195612930065877
1110	23	2.3524264192083812
1110	24	1.4344581935604381
1110	25	1.7373847300079532
1111	1	0.4372123173974636
1111	2	0.762501142868981
1111	3	0.5542286835301447
1111	4	1.0413133581886669
1111	5	0.8056581759809873
1111	6	0.3785052583819777
1111	7	0.2584657200152307
1111	8	-0.05040553988492036
1111	9	0.10950960949462996
1111	10	0.5003336394829958
1111	11	0.17885405149554146
1111	12	0.38777378678637575
1111	13	0.27621154289169425
1111	14	-0.6054556868341834
1111	15	-0.0031632248547595054
1111	16	0.5100357558665252
1111	17	1.0687004989153408
1111	18	1.8303120877379015
1111	19	0.8438005228302146
1111	20	0.6681305897873671
1111	21	0.6135603542237149
1111	22	0.9626757014614284
1111	23	0.7141594829717341
1111	24	0.6411624145922232
1111	25	0.11518757868938678
1112	1	0.43339395012320736
1112	2	0.72896583379449
1112	3	0.43563825710776527
1112	4	1.0352192184281108
1112	5	0.9171887281439837
1112	6	0.310251383554899
1112	7	0.3299870125576163
1112	8	0.31401203275095924
1112	9	0.47965569269856445
1112	10	0.7428466646897647
1112	11	0.5155051312052663
1112	12	0.5123011825319423
1112	13	0.28110995043741127
1112	14	0.6833966000383356
1112	15	0.5257136974708416
1112	16	0.9433964500778913
1112	17	1.199320328365154
1112	18	1.2809436164688535
1112	19	0.7053485087561029
1112	20	0.45136995140669
1112	21	0.5240472051146128
1112	22	0.5041967402279326
1112	23	2.2272564507338792
1112	24	1.1691821502515936
1112	25	1.076612579281175
1113	1	0.3548767318922401
1113	2	0.8238182075043561
1113	3	0.4275249153344447
1113	4	0.5372928472342018
1113	5	0.7789004152349577
1113	6	0.21826771259036803
1113	7	0.19559113263392902
1113	8	0.23485717260694372
1113	9	0.2486192349444778
1113	10	0.6173559294378848
1113	11	0.2735073103515191
1113	12	0.2350224436426629
1113	13	0.2537899014688776
1113	14	0.9229586562187748
1113	15	0.3966556284780509
1113	16	0.7583728785361146
1113	17	1.2575846339453634
1113	18	1.9453745091907422
1113	19	0.8613260825006606
1113	20	0.5860619132777458
1113	21	0.515584073014999
1113	22	0.8586087305549412
1113	23	0.6696272546480735
1113	24	0.7534898492474653
1113	25	1.145953367492254
1114	1	-0.14585707580392793
1114	2	-2.9872975249249514
1114	3	-0.9516022660104575
1114	4	0.24007847751743228
1114	5	0.5053255289081804
1114	6	0.3339187085057844
1114	7	0.26201932647443016
1114	8	0.22414073060785
1114	9	0.40890394656728546
1114	10	0.566218855697123
1114	11	0.2769792757597216
1114	12	0.28771240837015455
1114	13	0.22776142070108563
1114	14	1.1375499401138214
1114	15	0.5703590258726692
1114	16	0.6969797869692371
1114	17	1.160809214747509
1114	18	1.3782090923611818
1114	19	0.7179181474305025
1114	20	0.5388246771077903
1114	21	0.3598461835101858
1114	22	0.491610108091983
1114	23	2.20624095545838
1114	24	1.1874125233876498
1114	25	1.1159023120653586
1115	1	-0.14823878624967435
1115	2	0.20118134417438327
1115	3	-0.28558509586680714
1115	4	-0.19521208345673102
1115	5	-0.06660035215561777
1115	6	-0.5260622284364729
1115	7	-0.7077097832566679
1115	8	-0.8655045204175345
1115	9	-0.7099807005066765
1115	10	-0.5126504445105482
1115	11	-0.5129166340526652
1115	12	-0.7399273513964815
1115	13	-0.9543021636693538
1115	14	-0.07761654024108111
1115	15	-0.7162852164243532
1115	16	-0.2392366413422522
1115	17	0.23104255474320468
1115	18	0.7059038584570629
1115	19	-0.21252546204263467
1115	20	-0.6581019990870587
1115	21	-0.5627432051227572
1115	22	-0.2583390872186513
1115	23	1.5042872096545814
1115	24	0.3263250988121058
1115	25	0.08759631617459718
1116	1	-0.12703643876615445
1116	2	-0.4398201499923644
1116	3	-0.23061981815557075
1116	4	0.5537051726679785
1116	5	-0.1513219701740276
1116	6	-0.3366744535184161
1116	7	-0.47189475330625896
1116	8	-0.5178730525887545
1116	9	-0.468010521238611
1116	10	0.11428275748431105
1116	11	-0.2796869788991927
1116	12	-0.21868533407334212
1116	13	-0.4568974399560517
1116	14	0.20478812917372835
1116	15	-0.461553741632585
1116	16	-0.02038867648168008
1116	17	0.32320766559836756
1116	18	0.7734944938196904
1116	19	0.0066868776410657915
1116	20	-0.19673448197823973
1116	21	-0.41843953218109675
1116	22	0.0692016829639357
1116	23	1.4419464080427495
1116	24	0.4337266221083951
1116	25	0.5950636364673514
1201	1	1.0477512993328695
1201	2	1.4072633025892287
1201	3	1.015565695035935
1201	4	1.638079410804103
1201	5	1.4605600588483512
1201	6	1.1253551783489613
1201	7	0.9900068854298141
1201	8	0.9436616990038758
1201	9	1.142242974060359
1201	10	1.422200709382217
1201	11	1.0756386483466471
1201	12	1.091942335762674
1201	13	0.9305787103921546
1201	14	1.9371995409671143
1201	15	1.3773690225950546
1201	16	1.8103296750303883
1201	17	2.0579629681709477
1201	18	2.7196368126418378
1201	19	1.803955165024071
1201	20	1.5392774322478147
1201	21	1.4743171960706718
1201	22	1.8068898302169885
1201	23	3.559277817504171
1201	24	2.1488401464509437
1201	25	2.2354678615656667
1202	1	1.090155348132182
1202	2	1.5070713507157194
1202	3	1.2400839601471993
1202	4	1.9367144746726268
1202	5	1.6800873278876978
1202	6	1.3082827165167075
1202	7	1.1214272550855853
1202	8	1.1977589810049238
1202	9	1.4142617833650775
1202	10	1.878649278153425
1202	11	1.4537539085205893
1202	12	1.487973928666392
1202	13	1.3795134517023726
1202	14	2.365954970533636
1202	15	1.876048240341703
1202	16	2.05015869934099
1202	17	2.3985207169910367
1202	18	3.120661998904417
1202	19	2.472882584458702
1202	20	2.044729261232547
1202	21	1.9451154041900338
1202	22	2.415351914774753
1202	23	4.02558197712106
1202	24	2.748340725274886
1202	25	2.9359336416917188
1203	1	1.1110552557187923
1203	2	1.5420389569389563
1203	3	1.1433343832483374
1203	4	1.9025026209680505
1203	5	1.557563620110372
1203	6	1.1959605631988453
1203	7	1.0847101762813907
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2211	19	-1.8860116527528064
2211	20	-0.9931605167130257
2211	21	-0.7384105969308976
2211	22	-0.44796698158314285
2211	23	-4.695084221130426
2211	24	-1.9596110302916188
2211	25	-1.3769104890776802
2212	1	0.5200070154061258
2212	2	0.727643994964005
2212	3	0.45465159728444604
2212	4	0.9092241421581377
2212	5	0.988588250559566
2212	6	0.4569163393848683
2212	7	0.3824399609236906
2212	8	0.16730140628025225
2212	9	0.049199899704007066
2212	10	-3.807857968053039
2212	11	-0.9158725562944596
2212	12	-0.1682679171474959
2212	13	0.22644806541433837
2212	14	0.8977965530628182
2212	15	0.5747124230424845
2212	16	0.8195265809569037
2212	17	1.2545674500273947
2212	18	1.8463246183522202
2212	19	1.215723355976941
2212	20	0.7186123581189676
2212	21	0.5760836807330136
2212	22	0.6882083188227597
2212	23	1.9810192765721588
2212	24	1.2814047011091105
2212	25	1.1896378460194583
2213	1	-1.3921849282018286
2213	2	-3.9890012906197434
2213	3	-1.776462604706019
2213	4	-3.231843516590775
2213	5	-1.1184713328919251
2213	6	-0.7644660939174095
2213	7	-0.7506723595314782
2213	8	-0.5937634166618722
2213	9	-0.4946646297546238
2213	10	-0.49197857536582035
2213	11	-0.5917882293034324
2213	12	-0.5752998177232129
2213	13	-0.9357598577397965
2213	14	-5.897146933359071
2213	15	-2.634158475728217
2213	16	-1.8841592926741448
2213	17	-0.5557503853967845
2213	18	0.1721932529787722
2213	19	-0.6617934225465518
2213	20	-0.715442980624907
2213	21	-0.9383715067678295
2213	22	-0.7904931967584261
2213	23	-5.572851824756171
2213	24	-3.1945663376514126
2213	25	-2.244841963486344
2214	1	-0.3333875156272086
2214	2	-0.14733631453735185
2214	3	-0.41381365314541796
2214	4	-0.04513439131570962
2214	5	-0.25146555164798556
2214	6	-0.5431346895130211
2214	7	-0.6139802509571659
2214	8	-0.6125810053898348
2214	9	-0.6559540189825483
2214	10	-0.08821239702857753
2214	11	-0.5823393597777411
2214	12	-0.42836886561940446
2214	13	-0.3447389111234642
2214	14	-0.007239222631895359
2214	15	-0.3122935118402026
2214	16	0.09132701359320805
2214	17	0.2729888821645048
2214	18	0.9779464498459305
2214	19	-0.13733061262919932
2214	20	-0.27310432056537337
2214	21	-0.33445212269479896
2214	22	0.24243040546095074
2214	23	1.6863146340517723
2214	24	0.7632966529644563
2214	25	0.5731344348485692
2215	1	-2.7961460798848883
2215	2	-4.6578376844235585
2215	3	-2.706271914645548
2215	4	-4.78421189781244
2215	5	-5.67378191494576
2215	6	-2.8562821547297617
2215	7	-2.232106314347206
2215	8	-2.598615220096569
2215	9	-2.2538036221246855
2215	10	-3.7067261480902047
2215	11	-2.5291181379690393
2215	12	-2.059318531118869
2215	13	-2.127613838114595
2215	14	-1.41415371972424
2215	15	-2.088397166092779
2215	16	-3.01182284998249
2215	17	-7.279956782307895
2215	18	-3.6198334033745034
2215	19	-2.947385774885234
2215	20	-2.5220834379128476
2215	21	-2.4656756525712957
2215	22	-2.2172399753702337
2215	23	-6.597194306183944
2215	24	-3.9680614107415977
2215	25	-4.0087987553356506
2216	1	-2.0489325766268145
2216	2	-1.5887845632583293
2216	3	-1.8298545819136187
2216	4	-4.1211989328347975
2216	5	-2.5239094713646324
2216	6	-2.1813558622796485
2216	7	-2.321402525652168
2216	8	-2.242096943996682
2216	9	-2.352813523114783
2216	10	-1.8871734359896202
2216	11	-2.6154286946852565
2216	12	-3.4302421438563186
2216	13	-2.7135228544528616
2216	14	-8.229136113619699
2216	15	-4.856520194248706
2216	16	-3.0443518529947475
2216	17	-2.2898131642739017
2216	18	-1.521242845707004
2216	19	-1.8198020805552098
2216	20	-2.570451821549238
2216	21	-2.8020632993968846
2216	22	-2.3001433843855636
2216	23	-1.3561410920403176
2216	24	-1.95497174530394
2216	25	-1.910391735950327
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113058.0
3	2.0
4	960.0
5	5558.0
6	6800.0
7	6057.0
8	5541.0
9	5328.0
10	5014.0
11	4917.0
12	4805.0
13	4728.0
14	4740.0
15	4957.0
16	4939.0
17	5181.0
18	5869.0
19	6935.0
20	8617.0
21	10860.0
22	13331.0
23	16028.0
24	19218.0
25	22177.0
26	24853.0
27	27815.0
28	31908.0
29	37763.0
30	44205.0
31	50670.0
32	64286.0
33	82673.0
34	105000.0
35	135604.0
36	185542.0
37	250245.0
38	547595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.688734443480946	17.104864549489406	11.689943007293593	25.51645799973606
2	14.170810912403265	19.633793551955865	39.96960827133428	26.225787264306593
3	20.429662057551653	24.29533927713416	27.83626074422081	27.438737921093377
4	11.476432031552793	15.978513520872015	37.04613229495542	35.49892215261977
5	12.84591310810075	37.81823151739963	35.356251661696255	13.979603712803362
6	34.00177537123461	33.837956540235965	17.931836633819273	14.228431454710153
7	29.195813621051247	30.747156653992803	20.939421130142964	19.117608594812985
8	27.124396529819595	34.82644827505215	20.082238492696437	17.966916702431817
9	27.787164371444327	14.902583172782544	18.853440484636504	38.456811971136624
10	15.139297596726731	29.01268050175323	33.58122739056714	22.266794510952895
11	34.543419346608	23.1105806416486	21.65568036178207	20.690319649961328
12	24.362032677292916	25.329092228026845	29.758072307678347	20.55080278700189
13	29.48436010457555	20.56757538652709	24.249764342041914	25.698300166855443
14	21.833060207006625	21.79462679556094	27.05514660745074	29.31716638998169
15	26.956794673946288	26.979694740511285	22.110040958557285	23.95346962698514
16	22.56921831928718	27.09726116023291	27.891667752067423	22.44185276841249
17	24.163582069155485	27.163444343255648	27.023689922414412	21.64928366517446
18	23.78978664801994	25.677340493106225	28.731524529873013	21.801348329000817
19	24.146897975831266	27.1251209184033	27.42338631321123	21.3045947925542
20	24.362077267093213	26.359786179381707	26.824195850633036	22.453940702892044
21	23.147991954150616	26.327415676594434	27.76223746359296	22.76235490566199
22	23.59280804146299	26.667296641061945	25.051198766531492	24.688696550943572
23	21.753808489955155	26.43455301457521	28.033859887286738	23.777778608182896
24	23.031599269200832	28.5342692915925	25.769533448156935	22.66459799104974
25	23.455998582106243	25.643003607194316	25.497530697453186	25.403467113246258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	203.0
2	302.5
3	402.0
4	402.0
5	402.0
6	808.5
7	1215.0
8	1215.0
9	1215.0
10	1780.5
11	2346.0
12	2346.0
13	2346.0
14	3966.0
15	5586.0
16	5586.0
17	5586.0
18	10146.0
19	14706.0
20	14706.0
21	14706.0
22	23079.5
23	31453.0
24	31453.0
25	31453.0
26	45582.0
27	59711.0
28	59711.0
29	59711.0
30	77835.5
31	95960.0
32	95960.0
33	95960.0
34	115125.5
35	134291.0
36	134291.0
37	134291.0
38	154597.5
39	174904.0
40	174904.0
41	174904.0
42	198635.5
43	222367.0
44	222367.0
45	222367.0
46	271340.0
47	320313.0
48	320313.0
49	320313.0
50	300883.5
51	281454.0
52	281454.0
53	281454.0
54	242240.0
55	203026.0
56	203026.0
57	203026.0
58	172853.0
59	142680.0
60	142680.0
61	142680.0
62	117089.5
63	91499.0
64	91499.0
65	91499.0
66	71408.5
67	51318.0
68	51318.0
69	51318.0
70	37631.5
71	23945.0
72	23945.0
73	23945.0
74	16972.5
75	10000.0
76	10000.0
77	10000.0
78	7058.5
79	4117.0
80	4117.0
81	4117.0
82	2868.0
83	1619.0
84	1619.0
85	1619.0
86	1064.5
87	510.0
88	510.0
89	510.0
90	321.0
91	132.0
92	132.0
93	132.0
94	75.0
95	18.0
96	18.0
97	18.0
98	11.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11399423304455862
2	0.04808464605484425
3	0.036236930822685066
4	0.032074220065439946
5	0.03698408403552394
6	0.019639455880335943
7	0.01974619205359864
8	0.023855534724212406
9	0.028818766780927742
10	0.035596513783108893
11	0.0206000814397002
12	0.019639455880335943
13	0.01606379407603565
14	0.021827547432221196
15	0.022521332558428718
16	0.023001645338110843
17	0.026310466709254396
18	0.018625462234340334
19	0.03282137327827882
20	0.023108381511373538
21	0.027484564615144046
22	0.03666387551573585
23	0.028285085914614264
24	0.04061311392645557
25	0.03084675407291895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1873779.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.53875038674072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.59086905832937	60.37472947889705
2	9.815510316848316	13.847476643172834
3	2.4507422969442607	5.186168974391364
4	0.9032698930747044	2.5486211807781824
5	0.4150097530903461	1.4637134690651412
6	0.22773433110931454	0.9638457081966781
7	0.13137324346352053	0.6486833099718853
8	0.0924971054898359	0.5219704188514848
9	0.06320549842811361	0.40125931890212146
>10	0.2757233967919807	3.312028047324304
>50	0.01721393696823686	0.8190661190498346
>100	0.012523176362513974	1.7536383777857274
>500	0.0015457118212832644	0.7380960525529692
>1k	0.0022412821408607313	3.186313611312752
>5k	3.0914236425665255E-4	1.3185855102369932
>10k+	2.3185677319248943E-4	2.9158037795107115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	23936	1.2774185216079377	No Hit
CCCATGTACTCTGCGTTGATACCAC	19252	1.0274424038267054	No Hit
GAGTACATGGGAAGCAGTGGTATCA	10297	0.5495311880429868	No Hit
GCGTTGATACCACTGCTTCCCATGT	6591	0.3517490589872124	No Hit
GTATCAACGCAGAGTACATGGGAAG	6362	0.33952776714863386	No Hit
CATGTACTCTGCGTTGATACCACTG	6201	0.3309355052009869	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5033	0.2686015800155728	No Hit
TATCAACGCAGAGTACATGGGAAGC	5000	0.26684043315673833	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4472	0.23866208341538678	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4287	0.22878898738858747	No Hit
GGTATCAACGCAGAGTACATGGGAA	3283	0.1752074284107144	No Hit
CAGTGGTATCAACGCAGAGTACATG	2494	0.13310000805858108	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2166	0.11559527564349904	No Hit
CCACTGCTTCCCATGTACTCTGCGT	2089	0.11148593297288527	No Hit
GTGGTATCAACGCAGAGTACATGGG	2039	0.1088175286413179	No Hit
ATACCACTGCTTCCCATGTACTCTG	2026	0.10812374351511038	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2005	0.10700301369585208	No Hit
GTACTCTGCGTTGATACCACTGCTT	1949	0.1040144008444966	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1922	0.10257346250545023	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1905	0.1016662050327173	No Hit
CCCATGTACTCTGCGTTTATACCAC	1904	0.10161283694608596	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	5.3368086631347666E-5	0.0	0.0	0.0	0.0
6	5.3368086631347666E-5	0.0	0.0	0.0	0.0
7	5.3368086631347666E-5	0.0	0.0	0.0	0.0
8	5.3368086631347666E-5	0.0	0.0	5.3368086631347666E-5	0.0
9	5.3368086631347666E-5	0.0	0.0	5.3368086631347666E-5	0.0
10	5.3368086631347666E-5	0.0	0.0	1.60104259894043E-4	0.0
11	5.3368086631347666E-5	0.0	0.0	1.60104259894043E-4	0.0
12	5.3368086631347666E-5	0.0	0.0	1.60104259894043E-4	0.0
13	5.3368086631347666E-5	0.0	0.0	2.1347234652539066E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAC	150	0.0	14.566708	19
CTAGACG	60	2.563578E-5	14.253085	4
GTATCGA	145	0.0	13.758659	18
GCGTTTA	310	0.0	13.481749	13
GGATCGT	50	0.001501225	13.298262	6
CGTTTAT	325	0.0	13.151776	14
TGCGTTT	340	0.0	12.571215	12
ACCGCGC	55	0.003074313	12.088038	8
GTAGGAC	525	0.0	11.945442	3
GTCCTAC	540	0.0	11.803144	1
GGTATCG	155	2.5465852E-11	11.64364	17
GTCTAAT	115	6.9430826E-8	11.581021	1
TAGGACC	290	0.0	11.467999	4
ATACGAC	125	1.832268E-8	11.400031	19
TAGGACA	285	0.0	11.335787	4
GTCCTAT	235	0.0	11.334617	1
GTATAGG	205	0.0	11.13715	1
GTCTAAC	60	0.0058169896	11.098478	1
TGTTATC	500	0.0	11.018559	16
GTACCGA	70	0.0014932767	10.855724	6
>>END_MODULE