FastQCFastQC Report
Thu 26 May 2016
SRR1513094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873779
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC280981.4995364981676067No Hit
CCCATGTACTCTGCGTTGATACCAC271651.4497440733405593No Hit
GAGTACATGGGAAGCAGTGGTATCA109860.5863017997319855No Hit
CATGTACTCTGCGTTGATACCACTG76780.40976016915548735No Hit
GCGTTGATACCACTGCTTCCCATGT71040.37912688742909384No Hit
GTATCAACGCAGAGTACATGGGAAG70370.3755512256247935No Hit
ACGCAGAGTACATGGGAAGCAGTGG65590.35004128021500935No Hit
TATCAACGCAGAGTACATGGGAAGC62290.33242981162666463No Hit
ACTCTGCGTTGATACCACTGCTTCC54800.2924571147397852No Hit
GCTTCCCATGTACTCTGCGTTGATA53550.28578610391086673No Hit
GGTATCAACGCAGAGTACATGGGAA40520.21624748703022076No Hit
CAGTGGTATCAACGCAGAGTACATG31030.16560117281707182No Hit
GTATCAACGCAGAGTACTTTTTTTT29530.15759595982236965No Hit
CCACTGCTTCCCATGTACTCTGCGT28500.15209904689934084No Hit
ATACCACTGCTTCCCATGTACTCTG27060.14441404242442676No Hit
CATGGGAAGCAGTGGTATCAACGCA26330.1405181721003384No Hit
ACATGGGAAGCAGTGGTATCAACGC25120.13406063361794535No Hit
GCAGTGGTATCAACGCAGAGTACAT24050.12835024834839115No Hit
GGGAAGCAGTGGTATCAACGCAGAG23580.1258419482767178No Hit
GTGGTATCAACGCAGAGTACATGGG23430.1250414269772476No Hit
TACCACTGCTTCCCATGTACTCTGC23130.12344038437830715No Hit
CTGCTTCCCATGTACTCTGCGTTGA22830.12183934177936671No Hit
TATCAACGCAGAGTACTTTTTTTTT21220.11324707983171975No Hit
GTACTCTGCGTTGATACCACTGCTT21000.1120729819258301No Hit
AAGCAGTGGTATCAACGCAGAGTAC20510.10945794568089408No Hit
CTTCCCATGTACTCTGCGTTGATAC19830.10582891578996241No Hit
CCATGTACTCTGCGTTGATACCACT19270.10284030293860695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCG350.0021394216.3208265
TAGGACC2600.012.0836884
CGTGCGC802.9157189E-511.85549910
AGAACCG600.00578295611.1072295
GCGAATC600.005909852711.07488715
GTATTAA1657.6397555E-1110.9744451
GTATTAC1158.4623025E-710.77354051
GTCTAGA1951.8189894E-1210.7522911
GTCCTAC7150.010.5301361
CTAGGAC2200.010.385983
TGTACCG1201.4823145E-610.3138565
TCCTACA7800.010.2605232
GTATAGC3000.010.1658031
GTCCTAG2350.010.1387651
AACCGTG750.002679598310.11885457
CCTACAC2300.09.9344173
TCTATAC2300.09.9344173
GCCTAGA1659.6988515E-99.8192411
TCTAGAC1856.2391337E-109.7777923
TAGGACT2251.8189894E-129.7320484
Produced by FastQC (version 0.11.5)