FastQCFastQC Report
Thu 26 May 2016
SRR1513093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808866
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC310291.715384113582764No Hit
CCCATGTACTCTGCGTTGATACCAC244741.3530023782856222No Hit
GAGTACATGGGAAGCAGTGGTATCA137280.7589285220685225No Hit
GCGTTGATACCACTGCTTCCCATGT81550.450834942997436No Hit
GTATCAACGCAGAGTACATGGGAAG81040.44801549700198906No Hit
CATGTACTCTGCGTTGATACCACTG79530.43966772552527383No Hit
GCTTCCCATGTACTCTGCGTTGATA65780.36365325015783373No Hit
ACGCAGAGTACATGGGAAGCAGTGG65500.3621053190230786No Hit
TATCAACGCAGAGTACATGGGAAGC60950.33695143808330746No Hit
ACTCTGCGTTGATACCACTGCTTCC54170.29946939132030787No Hit
GGTATCAACGCAGAGTACATGGGAA40660.22478171406837213No Hit
CCACTGCTTCCCATGTACTCTGCGT31760.1755796172850836No Hit
CAGTGGTATCAACGCAGAGTACATG31360.17336828709257623No Hit
GCAGTGGTATCAACGCAGAGTACAT27680.15302404932150862No Hit
GTGGTATCAACGCAGAGTACATGGG26830.1483249726624305No Hit
CTGCTTCCCATGTACTCTGCGTTGA26000.14373646251297773No Hit
ACATGGGAAGCAGTGGTATCAACGC24760.13688133891620496No Hit
ATACCACTGCTTCCCATGTACTCTG24420.1350017082525737No Hit
CATGGGAAGCAGTGGTATCAACGCA23540.13013678182905752No Hit
GGGAAGCAGTGGTATCAACGCAGAG23000.12715148606917262No Hit
CCCATGTACTCTGCGTTTATACCAC22740.12571412144404284No Hit
GTACTCTGCGTTGATACCACTGCTT22420.12394505729003695No Hit
AAGCAGTGGTATCAACGCAGAGTAC22360.12361335776116086No Hit
GTACATGGGAAGCAGTGTTATCAAC20690.11438105420744267No Hit
TACCACTGCTTCCCATGTACTCTGC20250.11194859099568459No Hit
GATACCACTGCTTCCCATGTACTCT19860.10979254405798992No Hit
GTATCAACGCAGAGTACTTTTTTTT19170.10597799947591476No Hit
CTTCCCATGTACTCTGCGTTGATAC18330.10133420607164931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG401.2868197E-518.9975686
CCGCGCG551.1298822E-515.5434649
GGGACCG602.565124E-514.2521165
TCCAACG2000.013.77323518
GGACCGT655.460009E-513.1521616
GTACCGG604.045696E-412.6839861
TGGACCG751.4766434E-512.6685485
CGTTTAT4800.012.66469414
GCGTTTA4900.012.60007913
CCAACGA1457.2759576E-1212.44668219
GTATCGA1001.4453326E-712.34841818
TAGAACA5550.012.3268374
CGGTTTC1405.2750693E-1112.21238413
CGTTTCC1251.4151738E-912.15810714
CGCGCGG550.003071723312.08936110
GGACCGA550.003071723312.0893616
TAGGACC3550.012.0447084
TGCGTTT4900.012.01787212
GCGAATG802.8715398E-511.87315114
AGTGCAA907.472987E-611.60962410
Produced by FastQC (version 0.11.5)