FastQCFastQC Report
Thu 26 May 2016
SRR1513093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808866
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC351461.942985273646583No Hit
CCCATGTACTCTGCGTTGATACCAC329641.822357211645307No Hit
GAGTACATGGGAAGCAGTGGTATCA148600.8215091665164804No Hit
CATGTACTCTGCGTTGATACCACTG99720.5512846169920823No Hit
GTATCAACGCAGAGTACATGGGAAG86990.4809090336155359No Hit
GCGTTGATACCACTGCTTCCCATGT86060.4757676909179563No Hit
ACGCAGAGTACATGGGAAGCAGTGG83060.4591827144741512No Hit
GCTTCCCATGTACTCTGCGTTGATA79620.440165274818588No Hit
TATCAACGCAGAGTACATGGGAAGC74010.4091513688686725No Hit
ACTCTGCGTTGATACCACTGCTTCC67570.3735489527693041No Hit
GGTATCAACGCAGAGTACATGGGAA50090.2769138233567329No Hit
CCACTGCTTCCCATGTACTCTGCGT40070.22152000203442376No Hit
CAGTGGTATCAACGCAGAGTACATG39040.21582582678871734No Hit
ACATGGGAAGCAGTGGTATCAACGC34260.18940043098825451No Hit
CATGGGAAGCAGTGGTATCAACGCA33140.18320870644923395No Hit
ATACCACTGCTTCCCATGTACTCTG32070.17729339818427678No Hit
GCAGTGGTATCAACGCAGAGTACAT30560.16894562670756152No Hit
GTATCAACGCAGAGTACTTTTTTTT29580.16352786773591851No Hit
GTGGTATCAACGCAGAGTACATGGG29320.16209050311078874No Hit
CTGCTTCCCATGTACTCTGCGTTGA27950.15451669720145106No Hit
GGGAAGCAGTGGTATCAACGCAGAG27870.1540744311629496No Hit
TACCACTGCTTCCCATGTACTCTGC26600.14705345780173879No Hit
AAGCAGTGGTATCAACGCAGAGTAC25920.14329419647447628No Hit
GTACTCTGCGTTGATACCACTGCTT24620.1361073733488274No Hit
CTTCCCATGTACTCTGCGTTGATAC24130.13339849386300587No Hit
CCATGTACTCTGCGTTGATACCACT23310.1288652669683658No Hit
GATACCACTGCTTCCCATGTACTCT22940.12681978654029652No Hit
TATCAACGCAGAGTACTTTTTTTTT22140.12239712615528181No Hit
GCAGAGTACATGGGAAGCAGTGGTA20040.11078764264461823No Hit
GGTATCAACGCAGAGTACTTTTTTT18690.10332440324490592No Hit
GTACATGGTAAGCAGTGGTATCAAC18670.10321383673528056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC5350.012.8120273
TCCAACG1501.8189894E-1212.65477118
CCAACGA1207.566996E-1012.65022219
TGTAGGA6400.012.205692
AGTACCG550.00302070112.11642555
TAGGACG4100.011.84201054
ATTGGAC1052.6471935E-711.7867273
GTATTAG1552.3646862E-1111.68205451
GTTCTAA1655.456968E-1211.5516341
TAGACAG2150.011.5126175
GATATAC4850.011.3968171
AATCCCG2350.011.30445319
TATTAGG1104.794201E-711.2584642
ACCGTGC1354.834874E-911.2433978
CCCGTTT1105.0192284E-711.21859112
ACTGTTC5950.011.00128658
GGACGTG4600.010.7617946
TAGGACC3450.010.7617944
TTAGGAC3650.010.6937533
ACTGCGG803.820759E-410.6724448
Produced by FastQC (version 0.11.5)