FastQCFastQC Report
Thu 26 May 2016
SRR1513092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845825
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC225121.2196172443216449No Hit
CCCATGTACTCTGCGTTGATACCAC182190.9870383162000732No Hit
GAGTACATGGGAAGCAGTGGTATCA94560.512291251879241No Hit
GCGTTGATACCACTGCTTCCCATGT61630.3338886405807701No Hit
GTATCAACGCAGAGTACATGGGAAG60350.3269540720274132No Hit
CATGTACTCTGCGTTGATACCACTG58340.31606463234596993No Hit
TATCAACGCAGAGTACATGGGAAGC46930.25424945485081196No Hit
ACGCAGAGTACATGGGAAGCAGTGG46210.2503487600395487No Hit
ACTCTGCGTTGATACCACTGCTTCC40610.22001002261861227No Hit
GCTTCCCATGTACTCTGCGTTGATA40480.21930573049991198No Hit
GGTATCAACGCAGAGTACATGGGAA31520.17076375062641366No Hit
GTATCAACGCAGAGTACTTTTTTTT22690.12292606287161567No Hit
CAGTGGTATCAACGCAGAGTACATG22120.11983801281269893No Hit
GTGGTATCAACGCAGAGTACATGGG20350.11024880473501009No Hit
ATACCACTGCTTCCCATGTACTCTG19130.1036392940825918No Hit
CCACTGCTTCCCATGTACTCTGCGT18870.10223070984519118No Hit
GCAGTGGTATCAACGCAGAGTACAT18580.10065959665732124No Hit
GGGAAGCAGTGGTATCAACGCAGAG18490.10017200980591336No Hit
CTGCTTCCCATGTACTCTGCGTTGA18490.10017200980591336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA3200.014.84074413
CGTTTAT3250.014.61242514
TGCGTTT3550.013.91305312
GCCCGCT500.001500797113.2987487
TCCAACG1251.0186341E-1012.91983518
AGGGGCG550.003070985412.089777
GTCCTAC10700.011.9122181
GTATCGA802.8674185E-511.87484718
CCAACGA802.8674185E-511.87484719
TAGGACC4750.011.8014474
GAGTTCG658.031012E-411.6912077
ATACGAC1001.9303316E-611.39985419
GTATAAG2550.010.8175631
TCCTACA12850.010.7945142
GTGTTAG2150.010.6180411
TATCGAC909.517608E-510.5554219
GTGCTAC1659.094947E-1010.376721
TTAGGAC4950.010.3646273
GTATTAT2151.8189894E-1210.1756231
CCCGCTC750.00265004210.1323798
Produced by FastQC (version 0.11.5)