##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513092_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1845825
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.433252881502852	31.0	30.0	34.0	25.0	34.0
2	29.162489401758023	31.0	30.0	34.0	20.0	34.0
3	29.51647420530115	31.0	30.0	34.0	24.0	34.0
4	32.361110072731705	35.0	33.0	37.0	19.0	37.0
5	32.600308263242724	35.0	33.0	37.0	24.0	37.0
6	33.08032559966411	37.0	35.0	37.0	27.0	37.0
7	33.080461040456164	37.0	35.0	37.0	27.0	37.0
8	33.065485081196755	37.0	35.0	37.0	27.0	37.0
9	34.440985467203014	39.0	35.0	39.0	27.0	39.0
10	33.93993092519605	39.0	34.0	39.0	23.0	39.0
11	34.31302425744586	39.0	35.0	39.0	27.0	39.0
12	34.15399292999066	39.0	35.0	39.0	25.0	39.0
13	34.236495875827885	39.0	35.0	39.0	26.0	39.0
14	34.55781777795836	40.0	34.0	41.0	17.0	41.0
15	35.103479473947964	40.0	34.0	41.0	25.0	41.0
16	34.66796689827042	39.0	34.0	41.0	18.0	41.0
17	34.21452521230344	39.0	33.0	41.0	17.0	41.0
18	33.61552422358566	39.0	32.0	41.0	11.0	41.0
19	34.513711213143175	39.0	34.0	41.0	18.0	41.0
20	34.798393130443024	39.0	34.0	41.0	21.0	41.0
21	34.8243825931494	40.0	35.0	41.0	19.0	41.0
22	34.42417889019815	40.0	34.0	41.0	16.0	41.0
23	32.7440808310647	39.0	31.0	41.0	8.0	41.0
24	33.84350845827746	39.0	33.0	41.0	2.0	41.0
25	33.56974848644915	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9375503636711393
1101	2	1.3716859286014618
1101	3	1.0547464498889987
1101	4	1.1970307955811208
1101	5	1.3724883113348127
1101	6	1.0060739439734192
1101	7	1.0058027621979377
1101	8	1.0691887206757613
1101	9	1.2045097175079533
1101	10	1.6633182337561934
1101	11	1.1797288351991924
1101	12	1.258907863776983
1101	13	1.0857855581873324
1101	14	0.33482644170162956
1101	15	0.8900241999921903
1101	16	1.6134507717951365
1101	17	2.314936355606598
1101	18	-4.2642483906224555
1101	19	-0.3966013329775606
1101	20	0.7368975845965835
1101	21	0.9990198742180141
1101	22	1.622077774050858
1101	23	3.3827270806544476
1101	24	2.4059782822179727
1101	25	2.523927418551658
1102	1	0.9286399830311076
1102	2	1.2233887047228436
1102	3	1.0237687201467338
1102	4	1.7036915820384166
1102	5	1.3486413707000793
1102	6	0.9683298324647751
1102	7	1.0223781199796917
1102	8	0.8275400673265239
1102	9	0.9402222580896833
1102	10	1.3714683281708204
1102	11	1.0538488742901606
1102	12	1.1728746033251198
1102	13	1.060222176155932
1102	14	1.8591185390315204
1102	15	1.3360608560579195
1102	16	1.674140122818791
1102	17	1.3364664109302566
1102	18	1.657570705978621
1102	19	1.494380827626692
1102	20	1.3475821734788482
1102	21	1.4341294862898337
1102	22	1.8167799952792478
1102	23	3.3147661467778136
1102	24	2.211221192838792
1102	25	2.4576290931690323
1103	1	0.8994885788797049
1103	2	1.1818515381856756
1103	3	0.9296452510232633
1103	4	1.427486305833142
1103	5	1.3264459735046827
1103	6	0.8853017494366924
1103	7	0.909629757231329
1103	8	0.7994570392434994
1103	9	0.9660899089573292
1103	10	1.28706661876911
1103	11	0.9932890637303515
1103	12	1.021974577425091
1103	13	0.8016196675534175
1103	14	1.6495827044956854
1103	15	1.1605188055158706
1103	16	1.315498764177434
1103	17	1.8376966621605106
1103	18	1.8618941603020716
1103	19	1.4152516234974897
1103	20	1.270885721782399
1103	21	1.2286359057962528
1103	22	1.5953208988201482
1103	23	3.054156386168053
1103	24	2.040553897171492
1103	25	2.1306366411765794
1104	1	0.6277150501351052
1104	2	1.0333651053667232
1104	3	0.8404153027832066
1104	4	1.457962355479637
1104	5	1.182585293307298
1104	6	0.7378912806598947
1104	7	0.5776707708631434
1104	8	0.6862075800546847
1104	9	0.7728389962048539
1104	10	1.100048300346124
1104	11	0.6219056835459895
1104	12	0.8586438142337514
1104	13	0.773746201025368
1104	14	1.628587511315061
1104	15	1.0684468636704878
1104	16	1.281052951673061
1104	17	1.7736974860142425
1104	18	2.4071014993400155
1104	19	1.4748242204524473
1104	20	1.005251864914662
1104	21	1.0849207077067078
1104	22	1.3941778646342442
1104	23	2.908217461996294
1104	24	1.8758301773763222
1104	25	1.958614799496317
1105	1	0.5425791484408613
1105	2	1.0193228903629112
1105	3	0.697513004185133
1105	4	0.8282905669315213
1105	5	0.9487933973226959
1105	6	0.6089245318830052
1105	7	0.44093015617878706
1105	8	0.41406955091483155
1105	9	0.6656166893664661
1105	10	1.1751385141939465
1105	11	0.8701989185813801
1105	12	0.826256896413291
1105	13	0.7293847129655262
1105	14	1.401991243668931
1105	15	0.785688570391514
1105	16	1.2175126000147998
1105	17	1.7194448159674849
1105	18	1.5613360582733833
1105	19	1.2034593308228452
1105	20	0.945202418157919
1105	21	0.7375273411582768
1105	22	1.4266701051693573
1105	23	2.83141425901416
1105	24	1.8272761618349378
1105	25	1.8486996239454427
1106	1	-0.5149822829595792
1106	2	-0.1207261591288642
1106	3	-0.31166235870539793
1106	4	-2.849744445081818
1106	5	-0.5143851173264054
1106	6	-0.6355837977646459
1106	7	-0.5965353278811278
1106	8	-0.4994772333749893
1106	9	-0.4771044816054797
1106	10	-0.31211150672480414
1106	11	-0.322203291235688
1106	12	-0.2913543016932607
1106	13	-0.41710332748536416
1106	14	0.3463887039332647
1106	15	-0.22824282008786412
1106	16	-1.2808482663801257
1106	17	-5.934965952607371
1106	18	-7.129823976152906
1106	19	-3.1354458614105205
1106	20	-1.7120194321753885
1106	21	-1.38985391006198
1106	22	-1.5864962566812153
1106	23	-5.364568045714272
1106	24	-2.4165214283248844
1106	25	-1.0146949515234382
1107	1	0.679591728962258
1107	2	0.9263277108286871
1107	3	0.6981267409259679
1107	4	0.8770934557492538
1107	5	0.9982980086419886
1107	6	0.5675798786767174
1107	7	0.5063821866305105
1107	8	0.5159087417920105
1107	9	0.4987621514647955
1107	10	0.8777520518850253
1107	11	0.6232650781038913
1107	12	0.6840120479074656
1107	13	0.5310316333196567
1107	14	1.3372672593275112
1107	15	0.8881288771012237
1107	16	-5.397081280791902
1107	17	-0.6613124818022911
1107	18	0.9911483024295435
1107	19	0.8775229315463449
1107	20	0.6600632852647976
1107	21	0.6166442814462343
1107	22	0.7218309604486421
1107	23	2.65958131368361
1107	24	1.6466583431317616
1107	25	1.3204511463875832
1108	1	0.22524565079599412
1108	2	0.6098712605489744
1108	3	0.3274521306737199
1108	4	0.8455667511482261
1108	5	0.5226543662912917
1108	6	0.260623200177136
1108	7	0.17587511181466908
1108	8	0.21859555125910646
1108	9	0.29346279987770885
1108	10	0.687749424479847
1108	11	0.2806569565451724
1108	12	0.34404655417583996
1108	13	0.26331211074028005
1108	14	0.9362316157954353
1108	15	0.5502692630037558
1108	16	0.7223304489420741
1108	17	0.8913723858781353
1108	18	0.770994642377417
1108	19	0.5928385461961412
1108	20	0.47034892927633365
1108	21	0.5753381638113595
1108	22	0.9399715151188062
1108	23	2.540664020371217
1108	24	1.3376277885191286
1108	25	1.4588675738069554
1109	1	0.24236717175829625
1109	2	0.4097385160726539
1109	3	0.352414673792687
1109	4	0.21855259689943551
1109	5	0.1456980427567558
1109	6	0.03970890384384518
1109	7	0.11506696266853567
1109	8	-0.036169796383340724
1109	9	0.0033911962586188338
1109	10	0.45673628843878333
1109	11	0.1268064472477306
1109	12	0.2326362880868018
1109	13	0.0026453685791238968
1109	14	-1.089262534349558
1109	15	-0.2978711528740874
1109	16	-0.3819351832565161
1109	17	0.6433887178525666
1109	18	1.419351717759632
1109	19	0.6442711025169672
1109	20	0.3823380832347567
1109	21	0.38724606440641196
1109	22	0.20841201191126402
1109	23	-4.216284134272467
1109	24	-1.4263875697699717
1109	25	-0.05568229514236123
1110	1	0.5590963322374556
1110	2	0.8408591971933355
1110	3	0.5886598475378619
1110	4	1.2099364945333306
1110	5	0.753657650716363
1110	6	0.512858220743162
1110	7	0.36338086723243634
1110	8	0.3378804214168767
1110	9	0.40808194469936865
1110	10	0.8474680229205163
1110	11	0.5202204656617511
1110	12	0.6170010787015912
1110	13	0.385695314129066
1110	14	1.2156763330893128
1110	15	0.6965105915076535
1110	16	1.1122887797174457
1110	17	1.382495613209457
1110	18	1.9542740026819132
1110	19	1.198402500398366
1110	20	0.940098453495132
1110	21	0.7579364663468127
1110	22	1.045374317623569
1110	23	2.531290363302034
1110	24	1.4737491741167688
1110	25	1.753798201838137
1111	1	0.5248582244548281
1111	2	0.8222832525899939
1111	3	0.5461997391530957
1111	4	1.1217261603469666
1111	5	0.7273216579420136
1111	6	0.2831436715936846
1111	7	0.15738865738748586
1111	8	0.05629548238331239
1111	9	0.3259626085560612
1111	10	0.48950193079346604
1111	11	0.30698472879587513
1111	12	0.37543382745968756
1111	13	0.2671739382446745
1111	14	-0.29132539805625157
1111	15	0.11139844338180893
1111	16	0.5268915570770716
1111	17	1.1417751179512905
1111	18	1.7886297154622852
1111	19	0.6851225440533426
1111	20	0.5866944944404864
1111	21	0.5731169194553516
1111	22	0.9467940451563095
1111	23	0.9592671810048756
1111	24	0.8139804325843301
1111	25	0.3607434825847875
1112	1	0.4233451358381899
1112	2	0.7815164911354238
1112	3	0.6360555393841096
1112	4	1.180325031242134
1112	5	0.9169261465280343
1112	6	0.3095300026524157
1112	7	0.30312911635886053
1112	8	0.27470954006322046
1112	9	0.3715797891339747
1112	10	0.7814038276339161
1112	11	0.4544999095500657
1112	12	0.3986878667696061
1112	13	0.33454482645216643
1112	14	0.7083851354620165
1112	15	0.42104384694168573
1112	16	0.7619350930214779
1112	17	1.1667380068638948
1112	18	1.2055466886819985
1112	19	0.6861744114368733
1112	20	0.6720861787735757
1112	21	0.7223965004711701
1112	22	0.6854847891872211
1112	23	2.2204786724530905
1112	24	1.2363840481320238
1112	25	1.2631314650869143
1113	1	0.19500989437438676
1113	2	0.5752865541240197
1113	3	0.2247083030769943
1113	4	0.37651338468711515
1113	5	0.5018724851495691
1113	6	-0.09859802440981724
1113	7	-0.08709130540184873
1113	8	-0.12454376391842459
1113	9	0.060190031353059226
1113	10	0.390410308664066
1113	11	0.06634029124229812
1113	12	0.06595970163657938
1113	13	0.02269671153325703
1113	14	0.7329472612290786
1113	15	0.2514154070649326
1113	16	0.5010575093233953
1113	17	0.9336293579600508
1113	18	1.5631837347607558
1113	19	0.5484554517745508
1113	20	0.3387985383636547
1113	21	0.3022971905094707
1113	22	0.5695668777265794
1113	23	0.47352123496033727
1113	24	0.42080831504242155
1113	25	0.8532397888795913
1114	1	-0.42092559120333917
1114	2	-2.9512811128277683
1114	3	-1.105471765881834
1114	4	-0.01514674176679165
1114	5	0.24267591721807236
1114	6	-0.06705817908217426
1114	7	-0.08602738180329084
1114	8	-0.10337067020672919
1114	9	-0.0076942906546833
1114	10	-0.00866553722794805
1114	11	-0.21877051024975458
1114	12	-0.07309198221100388
1114	13	-0.14695787705061747
1114	14	0.6249451272091022
1114	15	-0.006509567141641526
1114	16	0.31299788021959074
1114	17	0.66719696318399
1114	18	0.8936124787753599
1114	19	0.19476148082191713
1114	20	0.011560683318286635
1114	21	-0.13414516890534856
1114	22	0.008276853829080721
1114	23	1.6670010102709583
1114	24	0.5714035478886927
1114	25	0.6201170650940853
1115	1	0.34157932975513106
1115	2	0.5877199982152987
1115	3	0.4145313835315889
1115	4	0.4987985984136003
1115	5	0.5155249115928697
1115	6	0.198751876295006
1115	7	-0.019614563690666387
1115	8	0.08034861207694632
1115	9	-0.051109829510572524
1115	10	0.3485299976036913
1115	11	0.23871415823074216
1115	12	0.11751051807331692
1115	13	0.06842482042198128
1115	14	0.955949352672107
1115	15	0.28381298528296384
1115	16	0.8001294644620316
1115	17	1.1777050685493649
1115	18	1.6821871067509022
1115	19	0.7245671795105366
1115	20	0.3162135042303902
1115	21	0.38549273603485545
1115	22	0.7514876808125592
1115	23	2.3755897231231913
1115	24	0.9154240365726807
1115	25	0.788330297879483
1116	1	0.29454087212746316
1116	2	0.13130374147052137
1116	3	0.2338454867150368
1116	4	0.954038039347509
1116	5	0.4651180204328753
1116	6	0.1834163535238318
1116	7	-0.002233088039375275
1116	8	0.047759980136667934
1116	9	0.15677752610613993
1116	10	0.5544643262817956
1116	11	0.1458996012817977
1116	12	0.1974695462768139
1116	13	0.06147583750221486
1116	14	0.8357870392148712
1116	15	0.2536202815620925
1116	16	0.6360505198138213
1116	17	1.0093743137966413
1116	18	1.5335933578716023
1116	19	0.6946789901972963
1116	20	0.38832730465036036
1116	21	0.19104873694112
1116	22	0.5971104786997969
1116	23	2.0483222774115575
1116	24	0.9272418747250128
1116	25	1.102423566412881
1201	1	1.0864482101114987
1201	2	1.436688304240974
1201	3	1.205596244777979
1201	4	1.6782958554387548
1201	5	1.4658105106255164
1201	6	1.1198934579430286
1201	7	0.9167386846248249
1201	8	1.007343744941224
1201	9	1.1738985683347636
1201	10	1.5289086066891713
1201	11	1.085382273937384
1201	12	1.1942703184913555
1201	13	1.2148221568230397
1201	14	2.1057759498169872
1201	15	1.4519008597581902
1201	16	1.7591120745364535
1201	17	2.284655303664934
1201	18	2.8054462707640724
1201	19	2.06177115245044
1201	20	1.677405313909432
1201	21	1.5477529636947764
1201	22	1.8153078112550745
1201	23	3.5066011706996676
1201	24	2.3048203170380575
1201	25	2.4539570132930137
1202	1	0.8654958151075292
1202	2	1.2463011816794385
1202	3	0.9716026400394782
1202	4	1.5631645165113497
1202	5	1.3296232292554677
1202	6	0.8736654282415444
1202	7	0.709312509119961
1202	8	0.789157085928835
1202	9	0.95070072297991
1202	10	1.267785573017477
1202	11	0.8410147449119094
1202	12	1.05083672834607
1202	13	0.9400989986798081
1202	14	1.9199709355456775
1202	15	1.2471206432206472
1202	16	1.5434054571576539
1202	17	1.9103236675081021
1202	18	2.5672709322229608
1202	19	1.7747478462290047
1202	20	1.4726533985500723
1202	21	1.4701253803739647
1202	22	1.7348983964858817
1202	23	3.321872942487552
1202	24	2.2206585147761118
1202	25	2.2869874390714955
1203	1	0.9321042279940919
1203	2	1.2629603957428976
1203	3	1.0223953500705925
1203	4	1.6115362329561194
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2211	14	-0.16075048557138416
2211	15	-0.4838042318470883
2211	16	-0.7130625471372056
2211	17	-0.16531968348633796
2211	18	-5.496465721599261
2211	19	-1.9321746054538167
2211	20	-1.2022101292561373
2211	21	-1.040118312804431
2211	22	-0.7346168763581602
2211	23	-5.022950772566535
2211	24	-2.3413297144971743
2211	25	-1.7874708114753979
2212	1	0.059281008672133595
2212	2	0.30534027167441025
2212	3	0.04144678282479575
2212	4	0.6142328925797287
2212	5	0.47238780944651637
2212	6	0.0544836786186238
2212	7	-0.07445948685791848
2212	8	-0.1483573685709132
2212	9	-0.4647492518818268
2212	10	-3.8048991931967002
2212	11	-1.310481610040327
2212	12	-0.5523533169028028
2212	13	-0.26088893495518306
2212	14	0.38611284102582033
2212	15	0.1212602862573462
2212	16	0.10157602025469004
2212	17	0.7284892029530496
2212	18	1.2231262615341763
2212	19	0.4591244173702833
2212	20	0.03218331307999023
2212	21	0.0018360389963874013
2212	22	0.226138969638221
2212	23	1.4209484829601493
2212	24	0.6279021045196984
2212	25	0.4312615718015067
2213	1	-1.4112643358555133
2213	2	-3.489564803584649
2213	3	-1.536662286080734
2213	4	-3.3591125427126087
2213	5	-1.0800484661755974
2213	6	-0.8627343835551926
2213	7	-0.674524534887567
2213	8	-0.7095978193688808
2213	9	-0.5402034535307223
2213	10	-0.8691242896341862
2213	11	-0.7679371731596021
2213	12	-0.6232042358953152
2213	13	-0.9402127379958003
2213	14	-6.056939878133093
2213	15	-2.594236272248061
2213	16	-1.8185041552237209
2213	17	-0.5711931206230787
2213	18	0.12438486527065606
2213	19	-0.5493091353110557
2213	20	-0.6566692699318892
2213	21	-0.9426038672134283
2213	22	-0.8295497074663842
2213	23	-5.4031888742745515
2213	24	-2.8408203824328737
2213	25	-2.000742058786191
2214	1	-0.19630611303002254
2214	2	-0.06255264491679213
2214	3	-0.12190164361141953
2214	4	0.16423246134822023
2214	5	-0.14312031064282849
2214	6	-0.3675132682622788
2214	7	-0.4679903242232655
2214	8	-0.6192718381130291
2214	9	-0.43643942066584174
2214	10	0.04230823778469528
2214	11	-0.352868562699733
2214	12	-0.3381350965089993
2214	13	-0.3565695476489097
2214	14	0.22807339196720022
2214	15	-0.2526222635283304
2214	16	0.22547607803971204
2214	17	0.4913446455259489
2214	18	0.962112867483448
2214	19	-0.03034059681723278
2214	20	-0.188152185035527
2214	21	-0.2372307760139165
2214	22	0.21039625960690955
2214	23	1.5825530091660909
2214	24	0.5140280068716407
2214	25	0.5096884392495653
2215	1	-2.1400470864478933
2215	2	-4.135009132542951
2215	3	-2.112138999330508
2215	4	-3.6019155101642077
2215	5	-4.61831615740283
2215	6	-1.9872536526285458
2215	7	-1.3191908645536152
2215	8	-1.5861291614477402
2215	9	-1.2807558409727804
2215	10	-2.865132262339408
2215	11	-1.791983877177806
2215	12	-1.3694420900836093
2215	13	-1.4023244561973058
2215	14	-0.5652806434120095
2215	15	-1.2834781593213762
2215	16	-2.089833195922786
2215	17	-6.573932971959955
2215	18	-2.7069161742635117
2215	19	-1.7418542564326671
2215	20	-1.507915124290875
2215	21	-1.3873852113919156
2215	22	-1.1820069935460893
2215	23	-6.200652335546302
2215	24	-3.296993202462968
2215	25	-3.0546638221332216
2216	1	-0.7707038149270282
2216	2	-0.33307117048081025
2216	3	-0.6063009541894537
2216	4	-2.9960870599904723
2216	5	-1.134369688688345
2216	6	-0.7928758829394127
2216	7	-0.8245224901967205
2216	8	-0.7440159534495407
2216	9	-0.7434959932107148
2216	10	-0.285304956257626
2216	11	-0.8541358036912001
2216	12	-1.7037761257567396
2216	13	-0.9864140526077492
2216	14	-6.871734492891875
2216	15	-3.2485306765505406
2216	16	-1.4692889211246936
2216	17	-0.5753241868875563
2216	18	0.22008825812444854
2216	19	-0.2805024417388182
2216	20	-0.8737040524953983
2216	21	-1.0180335290577176
2216	22	-0.4716060157669091
2216	23	0.522948307084107
2216	24	-0.20891748920662678
2216	25	-0.10260313413090216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79819.0
3	3.0
4	740.0
5	4128.0
6	5472.0
7	4714.0
8	4581.0
9	4058.0
10	3999.0
11	3910.0
12	3789.0
13	3792.0
14	3680.0
15	3739.0
16	3894.0
17	4085.0
18	4727.0
19	5589.0
20	6852.0
21	8704.0
22	10722.0
23	12969.0
24	15679.0
25	18511.0
26	20929.0
27	24053.0
28	27276.0
29	32875.0
30	38644.0
31	44344.0
32	57290.0
33	75027.0
34	98613.0
35	129509.0
36	182844.0
37	257042.0
38	639223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.439934823666995	17.393608423455234	11.836642594382555	25.329814158495218
2	13.711637870057253	19.711743021901395	41.07263553340983	25.503983574631523
3	20.146154475655646	24.51745664764946	28.1992834268477	27.137105449847194
4	10.964526039292915	16.198776438557196	37.90298325068052	34.93371427146936
5	12.340530463152646	38.20050076415309	35.911672573949424	13.547296198744837
6	33.41046037801283	34.608115137853304	18.161251517253337	13.820172966880529
7	28.903663846239297	30.964499462195167	21.234973299412342	18.896863392153197
8	26.64018263899915	35.73403764734698	20.200889893675534	17.424889819978333
9	27.642136317770603	14.932428551145446	19.078949507561347	38.346485623522604
10	14.614440364735051	29.33683796929831	34.20580703727289	21.842914628693755
11	34.309826123521226	23.201845839059136	22.299409140761483	20.188918896658155
12	24.031874737872222	25.417589193887313	30.499589807279865	20.050946260960597
13	29.489679641641658	20.8973371833628	24.53136692121004	25.081616253785505
14	21.1205777753935	22.292124894942784	27.48676048797484	29.100536841688868
15	26.284542893417935	27.947142741799574	22.39860454757527	23.36970981720722
16	21.86160205306942	27.444685038516724	28.769469695984462	21.924243212429392
17	23.310116406164905	27.73578331253329	27.741148151379498	21.212952129922307
18	22.810743150063264	26.144934447060482	29.775096114508347	21.26922628836791
19	23.780586939745245	27.299548241088385	27.853082972764398	21.06678184640197
20	23.964116277998595	26.821375253670602	27.446820616612616	21.767687851718186
21	22.696080848718626	26.596861362482336	27.999759391716246	22.707298397082788
22	23.030893607473473	27.336531285718852	25.680016519080677	23.952558587726998
23	20.787907558480434	27.19991892996221	28.775582311558225	23.236591199999133
24	22.748525682473115	28.73852834358847	26.45181186185397	22.061134112084442
25	22.877386482061652	26.17820908642088	26.24264525369912	24.70175917781835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	183.0
1	183.0
2	333.0
3	483.0
4	483.0
5	483.0
6	935.5
7	1388.0
8	1388.0
9	1388.0
10	2065.5
11	2743.0
12	2743.0
13	2743.0
14	4595.5
15	6448.0
16	6448.0
17	6448.0
18	11561.5
19	16675.0
20	16675.0
21	16675.0
22	26808.0
23	36941.0
24	36941.0
25	36941.0
26	52520.0
27	68099.0
28	68099.0
29	68099.0
30	88252.0
31	108405.0
32	108405.0
33	108405.0
34	126995.5
35	145586.0
36	145586.0
37	145586.0
38	164691.0
39	183796.0
40	183796.0
41	183796.0
42	204339.0
43	224882.0
44	224882.0
45	224882.0
46	268729.5
47	312577.0
48	312577.0
49	312577.0
50	289915.0
51	267253.0
52	267253.0
53	267253.0
54	226780.0
55	186307.0
56	186307.0
57	186307.0
58	157006.0
59	127705.0
60	127705.0
61	127705.0
62	103882.5
63	80060.0
64	80060.0
65	80060.0
66	61749.5
67	43439.0
68	43439.0
69	43439.0
70	31567.0
71	19695.0
72	19695.0
73	19695.0
74	13952.0
75	8209.0
76	8209.0
77	8209.0
78	5731.0
79	3253.0
80	3253.0
81	3253.0
82	2203.5
83	1154.0
84	1154.0
85	1154.0
86	769.5
87	385.0
88	385.0
89	385.0
90	254.5
91	124.0
92	124.0
93	124.0
94	73.5
95	23.0
96	23.0
97	23.0
98	17.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11978383649587582
2	0.047350100903390084
3	0.035431311202307914
4	0.03012203215364404
5	0.034943724350900006
6	0.0208578819768938
7	0.01950347405631628
8	0.02362087413487194
9	0.028117508431189306
10	0.035214605935015506
11	0.020424471442308995
12	0.019015887204908374
13	0.016144542413284033
14	0.021778879362886513
15	0.021778879362886513
16	0.021291292511478606
17	0.02578792680779597
18	0.020045237224547287
19	0.032126555876098764
20	0.02275405306570233
21	0.027196511045196593
22	0.037164953340647136
23	0.027467392629312096
24	0.03976541654815597
25	0.03185567429198326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1845825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.97529329450688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.11214520280814	59.557473234911576
2	10.261737577226931	14.361361933554342
3	2.469622512295575	5.184376788737994
4	0.9060652807802454	2.5360873506626964
5	0.4087882373005943	1.4302538400226785
6	0.2238193657399564	0.9397095457586373
7	0.1370720536726412	0.671416001075642
8	0.0955610347231647	0.5349529145824006
9	0.0670994986722459	0.4225776389554305
>10	0.28059926389787776	3.4289481416175183
>50	0.01900234860382617	0.9131818768832964
>100	0.013905830870531532	1.9758841296714076
>500	0.0015009323234263224	0.7373530984089126
>1k	0.002606882456477296	3.5354143575184653
>5k	3.159857523002783E-4	1.5194813680983719
>10k+	1.5799287615013915E-4	2.2515277795407007
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	22512	1.2196172443216449	No Hit
CCCATGTACTCTGCGTTGATACCAC	18219	0.9870383162000732	No Hit
GAGTACATGGGAAGCAGTGGTATCA	9456	0.512291251879241	No Hit
GCGTTGATACCACTGCTTCCCATGT	6163	0.3338886405807701	No Hit
GTATCAACGCAGAGTACATGGGAAG	6035	0.3269540720274132	No Hit
CATGTACTCTGCGTTGATACCACTG	5834	0.31606463234596993	No Hit
TATCAACGCAGAGTACATGGGAAGC	4693	0.25424945485081196	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4621	0.2503487600395487	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4061	0.22001002261861227	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4048	0.21930573049991198	No Hit
GGTATCAACGCAGAGTACATGGGAA	3152	0.17076375062641366	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2269	0.12292606287161567	No Hit
CAGTGGTATCAACGCAGAGTACATG	2212	0.11983801281269893	No Hit
GTGGTATCAACGCAGAGTACATGGG	2035	0.11024880473501009	No Hit
ATACCACTGCTTCCCATGTACTCTG	1913	0.1036392940825918	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1887	0.10223070984519118	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1858	0.10065959665732124	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1849	0.10017200980591336	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1849	0.10017200980591336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.417631682310078E-5	0.0
6	0.0	0.0	0.0	5.417631682310078E-5	0.0
7	0.0	0.0	0.0	5.417631682310078E-5	0.0
8	0.0	0.0	0.0	5.417631682310078E-5	0.0
9	0.0	0.0	0.0	1.6252895046930236E-4	0.0
10	0.0	0.0	0.0	2.708815841155039E-4	0.0
11	0.0	0.0	0.0	2.708815841155039E-4	0.0
12	0.0	0.0	0.0	2.708815841155039E-4	0.0
13	0.0	5.417631682310078E-5	0.0	2.708815841155039E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTTA	320	0.0	14.840744	13
CGTTTAT	325	0.0	14.612425	14
TGCGTTT	355	0.0	13.913053	12
GCCCGCT	50	0.0015007971	13.298748	7
TCCAACG	125	1.0186341E-10	12.919835	18
AGGGGCG	55	0.0030709854	12.08977	7
GTCCTAC	1070	0.0	11.912218	1
GTATCGA	80	2.8674185E-5	11.874847	18
CCAACGA	80	2.8674185E-5	11.874847	19
TAGGACC	475	0.0	11.801447	4
GAGTTCG	65	8.031012E-4	11.691207	7
ATACGAC	100	1.9303316E-6	11.399854	19
GTATAAG	255	0.0	10.817563	1
TCCTACA	1285	0.0	10.794514	2
GTGTTAG	215	0.0	10.618041	1
TATCGAC	90	9.517608E-5	10.55542	19
GTGCTAC	165	9.094947E-10	10.37672	1
TTAGGAC	495	0.0	10.364627	3
GTATTAT	215	1.8189894E-12	10.175623	1
CCCGCTC	75	0.002650042	10.132379	8
>>END_MODULE