FastQCFastQC Report
Thu 26 May 2016
SRR1513092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845825
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC260581.4117264637763602No Hit
CCCATGTACTCTGCGTTGATACCAC240501.3029404195955738No Hit
GAGTACATGGGAAGCAGTGGTATCA100230.5430092235179391No Hit
CATGTACTCTGCGTTGATACCACTG70990.38459767312719245No Hit
GTATCAACGCAGAGTACATGGGAAG65850.35675104628011867No Hit
GCGTTGATACCACTGCTTCCCATGT65450.3545839936071946No Hit
ACGCAGAGTACATGGGAAGCAGTGG60650.3285793615321062No Hit
TATCAACGCAGAGTACATGGGAAGC55780.30219549523925615No Hit
ACTCTGCGTTGATACCACTGCTTCC50250.2722359920360814No Hit
GCTTCCCATGTACTCTGCGTTGATA49310.26714341825471No Hit
GGTATCAACGCAGAGTACATGGGAA38240.20717023553153738No Hit
GTATCAACGCAGAGTACTTTTTTTT36180.1960099142659786No Hit
CAGTGGTATCAACGCAGAGTACATG27160.1471428764915417No Hit
ATACCACTGCTTCCCATGTACTCTG24970.13527826310728264No Hit
TATCAACGCAGAGTACTTTTTTTTT24890.13484485257269785No Hit
CCACTGCTTCCCATGTACTCTGCGT24320.1317568025137811No Hit
CATGGGAAGCAGTGGTATCAACGCA23580.12774775506887165No Hit
ACATGGGAAGCAGTGGTATCAACGC22700.12298023918843877No Hit
GCAGTGGTATCAACGCAGAGTACAT22650.12270935760432328No Hit
GTGGTATCAACGCAGAGTACATGGG22280.12070483388186853No Hit
GGTATCAACGCAGAGTACTTTTTTT22070.11956713122858342No Hit
GGGAAGCAGTGGTATCAACGCAGAG21670.11740007855565938No Hit
TACCACTGCTTCCCATGTACTCTGC20810.11274091530887273No Hit
CTGCTTCCCATGTACTCTGCGTTGA20160.10921945471537117No Hit
GTACTCTGCGTTGATACCACTGCTT19480.10553546517140032No Hit
AAGCAGTGGTATCAACGCAGAGTAC19040.10315170723118389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.014.7475764
AACCGTG400.00532856314.2301837
AATCCCG903.7085556E-813.70276819
GTAGGAC9750.012.6941173
TGTAGGA9900.012.4137162
CTTAGGG1603.6379788E-1211.9007343
TACACCG657.8802346E-411.7176475
CGAAATC752.0890642E-411.39124613
TGGACCG855.207752E-511.2006915
CTACGCT600.00578242411.1073524
CCGCCGG600.005938740411.0676219
CTGTAGG10950.011.05236151
GTCCTAC10950.010.7912821
CTATACT2400.010.7106614
GGACGTG7950.010.6582676
TATACTG3050.010.6131135
AGGACCT7900.010.6052125
GACGTGG4350.010.468187
GGCGAGG2900.010.46789719
AGGACGT8050.010.40759855
Produced by FastQC (version 0.11.5)