##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1845825 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.598791326371675 31.0 31.0 34.0 27.0 34.0 2 30.718249563203447 31.0 31.0 34.0 27.0 34.0 3 30.738087846897727 33.0 31.0 34.0 27.0 34.0 4 33.964738802432514 37.0 35.0 37.0 30.0 37.0 5 33.92451667953354 37.0 35.0 37.0 30.0 37.0 6 33.88900031151382 37.0 35.0 37.0 30.0 37.0 7 33.80999607221703 37.0 35.0 37.0 29.0 37.0 8 33.810527000121894 37.0 35.0 37.0 29.0 37.0 9 35.368718052903176 39.0 35.0 39.0 29.0 39.0 10 35.27362398927309 39.0 35.0 39.0 29.0 39.0 11 35.30271883845977 39.0 35.0 39.0 29.0 39.0 12 35.21914536860211 39.0 35.0 39.0 29.0 39.0 13 35.19361152872022 39.0 35.0 39.0 29.0 39.0 14 36.38119810924654 40.0 37.0 41.0 28.0 41.0 15 36.36302087142606 40.0 37.0 41.0 28.0 41.0 16 36.29804504760744 40.0 37.0 41.0 28.0 41.0 17 36.23546760933458 40.0 36.0 41.0 27.0 41.0 18 36.15918843877399 40.0 36.0 41.0 27.0 41.0 19 36.196850730703076 40.0 37.0 41.0 27.0 41.0 20 36.1211100727317 40.0 37.0 41.0 27.0 41.0 21 36.14612707055111 40.0 37.0 41.0 27.0 41.0 22 36.139857245405175 40.0 37.0 41.0 27.0 41.0 23 36.08005200926415 40.0 37.0 41.0 27.0 41.0 24 35.95914076361519 40.0 37.0 41.0 27.0 41.0 25 35.93599718283153 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6959114772637633 1101 2 0.6010316783667378 1101 3 0.6813579896284914 1101 4 0.8785547939520129 1101 5 0.7299956869601019 1101 6 0.7163057796399244 1101 7 0.6801071739515478 1101 8 0.6972033052505537 1101 9 0.6922329488470993 1101 10 0.726065238347033 1101 11 0.7017909583971687 1101 12 0.7317067426210215 1101 13 0.7997437720718281 1101 14 0.8654673468292273 1101 15 0.9031083100561617 1101 16 0.8913601779025129 1101 17 0.8447803144944075 1101 18 0.8503937123457845 1101 19 0.9566585810099681 1101 20 1.1108470572184146 1101 21 1.058495450302317 1101 22 0.9992614218460858 1101 23 1.0376868261637568 1101 24 1.1231088176431925 1101 25 1.1285489277378105 1102 1 0.7023382976440722 1102 2 0.6594621179745062 1102 3 0.6862598895158563 1102 4 0.9264112091776155 1102 5 0.6735539590067461 1102 6 0.631241511436123 1102 7 0.6621031737527758 1102 8 0.6153886809213986 1102 9 0.7367154904226609 1102 10 0.6882569414544335 1102 11 0.7957645719928763 1102 12 0.6695856434359655 1102 13 0.7582354983687836 1102 14 0.7550094772870537 1102 15 0.738563902052455 1102 16 0.7216302120242872 1102 17 0.6743971070472412 1102 18 0.7472126018972318 1102 19 0.8339495851178356 1102 20 0.8622995103423818 1102 21 0.9943897804553643 1102 22 0.8545948131533123 1102 23 0.8554293226853247 1102 24 0.9665047627833232 1102 25 0.9901714514242883 1103 1 0.09717494248071645 1103 2 0.19901053252291945 1103 3 0.42390650216246684 1103 4 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2214 7 -0.2989706358326387 2214 8 -0.4233671730463655 2214 9 -0.44338978756354663 2214 10 -0.6710782145507537 2214 11 -0.5688050158360483 2214 12 -0.5174966010378057 2214 13 -0.524944532162408 2214 14 -0.6387228601889774 2214 15 -0.7895962343317038 2214 16 -0.5865540589500924 2214 17 -0.5455683369343447 2214 18 -0.5626204108873409 2214 19 -0.531672566957397 2214 20 -0.47677279568249986 2214 21 -0.6187481187572033 2214 22 -0.6679125968414823 2214 23 -0.6527964183860249 2214 24 -0.6033547419904082 2214 25 -0.6959106953036667 2215 1 -0.0011096227928426572 2215 2 0.0831682868874104 2215 3 0.06685458442365544 2215 4 0.40652281333056806 2215 5 0.10227675739443498 2215 6 0.07997739110731317 2215 7 -0.06985199363920458 2215 8 -0.10150733569953019 2215 9 -0.26196593139980706 2215 10 -0.2793766192101401 2215 11 -0.31172638653175966 2215 12 -0.3342122749584817 2215 13 -0.4762240650754421 2215 14 -0.48734029932413847 2215 15 -0.6267114651402181 2215 16 -0.5640453787946811 2215 17 -0.6065588431317792 2215 18 -0.5351451688316615 2215 19 -0.3857175356413194 2215 20 -0.3596408040454193 2215 21 -0.28080309010262283 2215 22 -0.43950559448494175 2215 23 -0.34560421270966657 2215 24 -0.42958025700959723 2215 25 -0.5374426915616439 2216 1 -0.9842593329462552 2216 2 -0.4930785580892376 2216 3 -2.1725930818910477 2216 4 -0.5732469672147147 2216 5 -0.5724939123730195 2216 6 -0.5534632059187743 2216 7 -0.490409371599128 2216 8 -0.4555570925218859 2216 9 -0.6344898183863634 2216 10 -0.4684623272794397 2216 11 -0.5871466588274146 2216 12 -0.5072743875976471 2216 13 -0.621851432622563 2216 14 -0.7270854428264499 2216 15 -0.7628192306781756 2216 16 -0.6328388597946031 2216 17 -0.706057768927046 2216 18 -0.7820039790534992 2216 19 -0.5919400545537457 2216 20 -0.44913418202393984 2216 21 -0.4965355828563247 2216 22 -0.6952849576026594 2216 23 -0.7153614954503311 2216 24 -0.8020324307455695 2216 25 -0.8555034634507592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45714.0 3 0.0 4 785.0 5 5786.0 6 6871.0 7 5155.0 8 4162.0 9 3706.0 10 3324.0 11 2925.0 12 2750.0 13 2553.0 14 2522.0 15 2530.0 16 2540.0 17 2899.0 18 3064.0 19 3705.0 20 4741.0 21 6403.0 22 8036.0 23 9803.0 24 11115.0 25 12856.0 26 13880.0 27 15461.0 28 17526.0 29 20902.0 30 26557.0 31 33593.0 32 43101.0 33 55801.0 34 74839.0 35 102586.0 36 151182.0 37 252982.0 38 883470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.207603255001736 18.469165584181184 12.81020469425055 27.513026466566533 2 13.317918761774392 20.18042096417874 38.858056733936 27.643603540110867 3 18.090186279948682 24.484349537117296 28.73795118061094 28.687513002323083 4 10.693104709276339 16.900302032966287 36.238050736120705 36.16854252163667 5 12.645010406557459 38.20428497657238 35.515486290491694 13.635218326378471 6 33.7500176760892 35.02944020641321 17.615011513932565 13.605530603565022 7 28.47291937438611 30.85426776313615 21.44790099485156 19.224911867626176 8 26.641253476520077 36.23899598750512 19.77356825901944 17.34618227695536 9 26.930764219852353 14.772528969727974 19.110753185830063 39.18595362458961 10 14.605890552934847 29.575315220918764 33.96080441268653 21.857989813459863 11 34.27175639683685 22.888019868281614 22.184839166173646 20.65538456870789 12 23.637179318382415 25.478059360470816 30.636110311929905 20.24865100921686 13 29.586377174213986 20.60366252417148 24.67687833179008 25.13308196982446 14 21.1853690673035 22.129572843685192 26.887432359512577 29.797625729498726 15 26.26121111156258 28.075901019869164 22.34540110790568 23.317486760662575 16 22.05045624753806 27.38081240832922 28.30658230742431 22.262149036708408 17 23.40690949763296 28.040950630343175 27.049807515563494 21.502332356460368 18 23.56882752743294 26.37758329904385 28.52836051825095 21.525228655272258 19 23.662546422519306 27.33960555558265 27.838825456746648 21.159022565151396 20 23.80480273048637 26.705348556878363 27.55954654422819 21.93030216840708 21 22.45982148903607 26.78731732423171 27.951078785908738 22.80178240082348 22 22.996437907168882 27.199436566305042 25.504747199761624 24.299378326764455 23 21.459978264426365 27.4961507664336 27.02985444967066 24.014016519469376 24 22.732702683521318 28.72185331080316 26.551599532132315 21.99384447354321 25 22.848157328024055 26.084921376620212 26.01275852261184 25.054162772743894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 125.0 1 125.0 2 323.5 3 522.0 4 522.0 5 522.0 6 1094.5 7 1667.0 8 1667.0 9 1667.0 10 2290.0 11 2913.0 12 2913.0 13 2913.0 14 4505.5 15 6098.0 16 6098.0 17 6098.0 18 11242.0 19 16386.0 20 16386.0 21 16386.0 22 26145.0 23 35904.0 24 35904.0 25 35904.0 26 51740.5 27 67577.0 28 67577.0 29 67577.0 30 87252.0 31 106927.0 32 106927.0 33 106927.0 34 124422.0 35 141917.0 36 141917.0 37 141917.0 38 159463.5 39 177010.0 40 177010.0 41 177010.0 42 194963.0 43 212916.0 44 212916.0 45 212916.0 46 265939.0 47 318962.0 48 318962.0 49 318962.0 50 297260.5 51 275559.0 52 275559.0 53 275559.0 54 232481.0 55 189403.0 56 189403.0 57 189403.0 58 160202.5 59 131002.0 60 131002.0 61 131002.0 62 106652.0 63 82302.0 64 82302.0 65 82302.0 66 63384.5 67 44467.0 68 44467.0 69 44467.0 70 32492.5 71 20518.0 72 20518.0 73 20518.0 74 14511.5 75 8505.0 76 8505.0 77 8505.0 78 5910.0 79 3315.0 80 3315.0 81 3315.0 82 2276.5 83 1238.0 84 1238.0 85 1238.0 86 836.5 87 435.0 88 435.0 89 435.0 90 290.5 91 146.0 92 146.0 93 146.0 94 78.5 95 11.0 96 11.0 97 11.0 98 5.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1957932089986862 2 0.06772039602887597 3 5.4176316823100786E-5 4 0.0 5 0.019178416155377676 6 0.3891484837403329 7 0.0011918789701082172 8 0.03190985060880636 9 0.03375184538079179 10 0.0030338737420936438 11 0.0037381658607939542 12 0.010076794929096746 13 0.008559858058049923 14 0.002004523722454729 15 0.0 16 0.012243847602020776 17 0.026058808391911475 18 0.06696192759335257 19 0.029580268985413025 20 0.0 21 0.0 22 0.0 23 1.6252895046930236E-4 24 2.1670526729240314E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1845825.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.00963019834569 #Duplication Level Percentage of deduplicated Percentage of total 1 81.76232636870394 53.15338601385891 2 12.198121244466442 15.859907024346956 3 3.204083879237254 6.2488892434108445 4 1.1748667200118728 3.0551060400126078 5 0.554406201919471 1.802087108322709 6 0.30632081161552194 1.194828161110932 7 0.18531463537308096 0.8433065141161685 8 0.11408060043382826 0.5933070117606729 9 0.08509146017204147 0.4978587922939509 >10 0.37293457579184414 4.185862087022744 >50 0.02091646019326801 0.928405359232837 >100 0.016445619981315968 2.087681490665668 >500 0.001585852785297873 0.7108720034690791 >1k 0.0027543758902541997 3.573676896456531 >5k 5.007956164098545E-4 2.0020642937179303 >10k+ 2.5039780820492727E-4 3.2627619602014493 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 26058 1.4117264637763602 No Hit CCCATGTACTCTGCGTTGATACCAC 24050 1.3029404195955738 No Hit GAGTACATGGGAAGCAGTGGTATCA 10023 0.5430092235179391 No Hit CATGTACTCTGCGTTGATACCACTG 7099 0.38459767312719245 No Hit GTATCAACGCAGAGTACATGGGAAG 6585 0.35675104628011867 No Hit GCGTTGATACCACTGCTTCCCATGT 6545 0.3545839936071946 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6065 0.3285793615321062 No Hit TATCAACGCAGAGTACATGGGAAGC 5578 0.30219549523925615 No Hit ACTCTGCGTTGATACCACTGCTTCC 5025 0.2722359920360814 No Hit GCTTCCCATGTACTCTGCGTTGATA 4931 0.26714341825471 No Hit GGTATCAACGCAGAGTACATGGGAA 3824 0.20717023553153738 No Hit GTATCAACGCAGAGTACTTTTTTTT 3618 0.1960099142659786 No Hit CAGTGGTATCAACGCAGAGTACATG 2716 0.1471428764915417 No Hit ATACCACTGCTTCCCATGTACTCTG 2497 0.13527826310728264 No Hit TATCAACGCAGAGTACTTTTTTTTT 2489 0.13484485257269785 No Hit CCACTGCTTCCCATGTACTCTGCGT 2432 0.1317568025137811 No Hit CATGGGAAGCAGTGGTATCAACGCA 2358 0.12774775506887165 No Hit ACATGGGAAGCAGTGGTATCAACGC 2270 0.12298023918843877 No Hit GCAGTGGTATCAACGCAGAGTACAT 2265 0.12270935760432328 No Hit GTGGTATCAACGCAGAGTACATGGG 2228 0.12070483388186853 No Hit GGTATCAACGCAGAGTACTTTTTTT 2207 0.11956713122858342 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2167 0.11740007855565938 No Hit TACCACTGCTTCCCATGTACTCTGC 2081 0.11274091530887273 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2016 0.10921945471537117 No Hit GTACTCTGCGTTGATACCACTGCTT 1948 0.10553546517140032 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1904 0.10315170723118389 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.417631682310078E-5 0.0 0.0 0.0 0.0 9 5.417631682310078E-5 0.0 0.0 5.417631682310078E-5 0.0 10 5.417631682310078E-5 0.0 0.0 1.0835263364620156E-4 0.0 11 5.417631682310078E-5 0.0 0.0 1.0835263364620156E-4 0.0 12 5.417631682310078E-5 0.0 0.0 1.0835263364620156E-4 0.0 13 5.417631682310078E-5 0.0 0.0 1.0835263364620156E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 510 0.0 14.747576 4 AACCGTG 40 0.005328563 14.230183 7 AATCCCG 90 3.7085556E-8 13.702768 19 GTAGGAC 975 0.0 12.694117 3 TGTAGGA 990 0.0 12.413716 2 CTTAGGG 160 3.6379788E-12 11.900734 3 TACACCG 65 7.8802346E-4 11.717647 5 CGAAATC 75 2.0890642E-4 11.391246 13 TGGACCG 85 5.207752E-5 11.200691 5 CTACGCT 60 0.005782424 11.107352 4 CCGCCGG 60 0.0059387404 11.06762 19 CTGTAGG 1095 0.0 11.0523615 1 GTCCTAC 1095 0.0 10.791282 1 CTATACT 240 0.0 10.710661 4 GGACGTG 795 0.0 10.658267 6 TATACTG 305 0.0 10.613113 5 AGGACCT 790 0.0 10.605212 5 GACGTGG 435 0.0 10.46818 7 GGCGAGG 290 0.0 10.467897 19 AGGACGT 805 0.0 10.4075985 5 >>END_MODULE