FastQCFastQC Report
Thu 26 May 2016
SRR1513091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228091
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44610.36324669751671496No Hit
CCCATGTACTCTGCGTTGATACCAC33710.2744910597016019No Hit
GTATCAACGCAGAGTACTTTTTTTT26500.2157820552385776No Hit
GAGTACATGGGAAGCAGTGGTATCA22660.18451401402664785No Hit
GGTATCAACGCAGAGTACTTTTTTT16350.13313345672266957No Hit
TATCAACGCAGAGTACTTTTTTTTT14450.11766229049801685No Hit
GTATCAACGCAGAGTACATGGGAAG13740.11188095996143609No Hit
GCGTTGATACCACTGCTTCCCATGT12360.10064400765089883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA307.711672E-419.0035862
TAGGACT1552.5465852E-1111.6478344
TCTATAC1303.2550815E-810.963163
CTACACT2100.010.8596344
CCTACAC1408.3200575E-910.8587493
CTAAGTC700.001489175810.8587493
GTAGGAC2800.010.8587493
GCGTTTA909.527589E-510.55367813
CTCGAAC1201.5233327E-610.29109318
TAGGACC1951.4551915E-1110.2331164
GTATTAA1606.035407E-910.1063621
TTAGACC1054.095633E-59.9538543
CTAAGAC1054.095633E-59.9538543
TCCAGTA1151.0232774E-59.9149142
TGTAGGA3550.09.9032762
GTGCTAA1252.5267E-69.8923441
GTATTAT1856.330083E-109.7689481
GGCCTAG1106.738818E-59.5118711
TAGTACA1502.635552E-79.502184
GTAAGAC1201.6959391E-59.5014063
Produced by FastQC (version 0.11.5)