##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513091_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1228091 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.14391523103744 31.0 30.0 34.0 23.0 34.0 2 28.907887933386043 31.0 30.0 34.0 16.0 34.0 3 29.23516009807091 31.0 30.0 34.0 22.0 34.0 4 32.11145835284193 35.0 32.0 37.0 19.0 37.0 5 32.31410945931531 35.0 33.0 37.0 20.0 37.0 6 32.77426509924753 36.0 33.0 37.0 26.0 37.0 7 32.759848415141875 37.0 33.0 37.0 26.0 37.0 8 32.73803325649321 37.0 35.0 37.0 25.0 37.0 9 34.07568168808338 39.0 35.0 39.0 25.0 39.0 10 33.577888772086105 38.0 34.0 39.0 18.0 39.0 11 33.93618876777047 39.0 34.0 39.0 25.0 39.0 12 33.783637368892045 39.0 34.0 39.0 22.0 39.0 13 33.85185055504844 39.0 34.0 39.0 23.0 39.0 14 34.16610495476312 39.0 33.0 41.0 16.0 41.0 15 34.68897988829818 40.0 34.0 41.0 19.0 41.0 16 34.26430614669434 39.0 33.0 41.0 17.0 41.0 17 33.80905160936771 39.0 33.0 41.0 11.0 41.0 18 33.21424308133518 39.0 32.0 41.0 10.0 41.0 19 34.119204521489046 39.0 33.0 41.0 18.0 41.0 20 34.37939533796763 39.0 34.0 41.0 18.0 41.0 21 34.414086578274734 39.0 34.0 41.0 17.0 41.0 22 33.991181435251946 39.0 34.0 41.0 10.0 41.0 23 32.31813603389325 39.0 31.0 41.0 2.0 41.0 24 33.40121701079155 39.0 32.0 41.0 2.0 41.0 25 33.13813227195705 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8326154085399402 1101 2 1.1679360357243347 1101 3 1.0317416989320272 1101 4 1.0538145233780156 1101 5 1.2315298629382099 1101 6 0.8013663134501314 1101 7 0.8416694888390808 1101 8 0.7491832768193092 1101 9 0.927087412769275 1101 10 1.2502296833042408 1101 11 0.9173696410259993 1101 12 0.9667208791474522 1101 13 0.7618797149881544 1101 14 0.02033488101771752 1101 15 0.5675465360021335 1101 16 1.2934186453348602 1101 17 1.9148636328877444 1101 18 -4.2613895768404255 1101 19 -0.7593506675679791 1101 20 0.31259778270748484 1101 21 0.7810537095177068 1101 22 1.324652944173792 1101 23 3.0941196524759036 1101 24 2.0969395316798938 1101 25 2.2966230419543407 1102 1 0.9630739101896104 1102 2 1.2359874945416145 1102 3 0.9803209160368276 1102 4 1.5003824595983346 1102 5 1.2879138489869746 1102 6 1.045169547394515 1102 7 1.025262023654733 1102 8 0.9137308039031993 1102 9 1.1895984540939182 1102 10 1.4958846807951076 1102 11 1.2029425750777847 1102 12 1.2262907840728445 1102 13 1.258571291337347 1102 14 2.0659091547880735 1102 15 1.5363787782595821 1102 16 1.8923784333414275 1102 17 1.5088551086445179 1102 18 1.9468549149861545 1102 19 1.7386721121891 1102 20 1.5285683633960119 1102 21 1.4650759760944538 1102 22 1.8897797292352507 1102 23 3.3044906636909346 1102 24 2.3673952176498645 1102 25 2.522957708194113 1103 1 0.7334717334425207 1103 2 0.9922860375624367 1103 3 0.7804929396496227 1103 4 1.1828307721832516 1103 5 1.1604677627249913 1103 6 0.6727969527413578 1103 7 0.6887234738401773 1103 8 0.7059706913476518 1103 9 0.954050044193437 1103 10 1.2316947410205827 1103 11 0.9728331639287262 1103 12 1.0649305696113842 1103 13 0.8373819758513221 1103 14 1.6717977789664005 1103 15 1.0925090847117858 1103 16 1.3761423421218595 1103 17 1.872751609874733 1103 18 1.6675804896987998 1103 19 1.1178757312262277 1103 20 1.1714730492320058 1103 21 1.216431852668947 1103 22 1.4544584272265055 1103 23 3.0139647006341264 1103 24 1.809139002307056 1103 25 2.056555675584221 1104 1 0.5813005167145597 1104 2 0.9573794131951061 1104 3 0.6890204357352658 1104 4 1.286597658539442 1104 5 1.022977662408202 1104 6 0.6750528288291875 1104 7 0.5740248338518299 1104 8 0.4615287376281003 1104 9 0.6414282894688981 1104 10 1.03906852574913 1104 11 0.7118541876697577 1104 12 0.688606060271546 1104 13 0.5845201708773615 1104 14 1.5210193452555885 1104 15 0.9773683726628519 1104 16 1.3307039340819529 1104 17 1.787959685285287 1104 18 2.351465967432482 1104 19 1.4822443817742368 1104 20 0.9421303881971568 1104 21 1.017243968060562 1104 22 1.4792320999862199 1104 23 2.862171833867933 1104 24 1.7776375275956227 1104 25 1.816426911428806 1105 1 0.7840293972494337 1105 2 1.2044010867183168 1105 3 0.9126025347119402 1105 4 1.1176255878026709 1105 5 1.1550036009524334 1105 6 0.8545997838071386 1105 7 0.8132764452855881 1105 8 0.8167057627801668 1105 9 1.025862851417692 1105 10 1.4373572177773966 1105 11 0.9678453723533451 1105 12 0.9935716948576285 1105 13 0.948115608968024 1105 14 1.7371620410758695 1105 15 1.050087685794324 1105 16 1.476937212972608 1105 17 2.12961323929094 1105 18 1.9179351783782224 1105 19 1.5337992680563133 1105 20 1.2624701127664437 1105 21 1.2570864244420576 1105 22 1.5633277153867553 1105 23 2.887704735982176 1105 24 1.9995697146070697 1105 25 2.051783343717581 1106 1 -0.7165063485799301 1106 2 -0.15744779921672958 1106 3 -0.32512967970320616 1106 4 -2.689259802285221 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sequence quality scores pass #Quality Count 2 60681.0 3 1.0 4 523.0 5 3115.0 6 3772.0 7 3449.0 8 3204.0 9 3079.0 10 2794.0 11 2816.0 12 2779.0 13 2785.0 14 2670.0 15 2806.0 16 2758.0 17 3042.0 18 3399.0 19 3950.0 20 4924.0 21 6282.0 22 7484.0 23 9362.0 24 11181.0 25 12736.0 26 14739.0 27 16874.0 28 18724.0 29 22569.0 30 26311.0 31 30007.0 32 38297.0 33 49742.0 34 64791.0 35 85503.0 36 119729.0 37 166534.0 38 414679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02058771502937 18.149404402360382 13.372069800635987 24.457938081974255 2 14.106790951697798 19.15094162856079 41.61454858285248 25.127718836888928 3 19.75312563891407 23.647253453496013 28.67841413279964 27.921206774790274 4 11.136397327733803 16.272494913343575 38.856842184142664 33.73426557477996 5 12.98301746474835 37.303174379496824 35.557514093736685 14.156294062018143 6 32.72519517919999 35.75236633910116 17.733738595882546 13.7886998858163 7 28.865217844554046 31.30762113877288 20.758758413093478 19.068402603579596 8 26.125144364138077 36.001198894280655 19.44065717650623 18.432999565075036 9 26.151337491631004 16.367310502335172 19.624335978288666 37.85701602774515 10 14.941824662693822 29.2741197131472 33.298986370908096 22.485069253250877 11 34.87767513114526 23.12016985968007 22.320796652975925 19.681358356198746 12 23.4832245811439 25.10383852335919 30.088087644600236 21.324849250896676 13 28.79974916728425 21.193491379661374 25.145083923088823 24.861675529965552 14 21.430752979445835 21.348412523242246 27.737331501373564 29.48350299593836 15 24.616290538410855 28.406920575297455 22.984125484498097 23.99266340179359 16 22.82949085812067 28.15408886221088 26.551480643193454 22.464939636474995 17 22.57274199276082 27.7506915009725 27.224124506012554 22.45244200025412 18 22.07971488397208 27.07339897104779 28.743959975599648 22.10292616938049 19 23.49385470400843 27.09917104278208 27.666498056915156 21.74047619629433 20 23.620779385785944 26.693071339039413 27.561037389244124 22.12511188593051 21 23.54816928298512 26.291903110015696 27.665317578678927 22.49461002832026 22 23.19414554358504 26.645466201168738 27.079711903920185 23.080676351326034 23 21.740362811791382 26.382698673339068 28.93404488232074 22.942893632548806 24 22.88586312372465 27.084607833953108 27.25314738861442 22.77638165370783 25 23.09605184277746 26.292729891049365 27.14455833289892 23.466659933274254 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 107.0 2 221.5 3 336.0 4 336.0 5 336.0 6 662.0 7 988.0 8 988.0 9 988.0 10 1618.5 11 2249.0 12 2249.0 13 2249.0 14 3846.0 15 5443.0 16 5443.0 17 5443.0 18 9360.0 19 13277.0 20 13277.0 21 13277.0 22 20285.5 23 27294.0 24 27294.0 25 27294.0 26 37953.0 27 48612.0 28 48612.0 29 48612.0 30 61870.5 31 75129.0 32 75129.0 33 75129.0 34 88177.5 35 101226.0 36 101226.0 37 101226.0 38 113483.5 39 125741.0 40 125741.0 41 125741.0 42 137327.5 43 148914.0 44 148914.0 45 148914.0 46 161584.0 47 174254.0 48 174254.0 49 174254.0 50 168997.5 51 163741.0 52 163741.0 53 163741.0 54 146722.5 55 129704.0 56 129704.0 57 129704.0 58 111664.5 59 93625.0 60 93625.0 61 93625.0 62 76546.0 63 59467.0 64 59467.0 65 59467.0 66 46059.5 67 32652.0 68 32652.0 69 32652.0 70 23997.5 71 15343.0 72 15343.0 73 15343.0 74 10823.5 75 6304.0 76 6304.0 77 6304.0 78 4377.0 79 2450.0 80 2450.0 81 2450.0 82 1642.5 83 835.0 84 835.0 85 835.0 86 563.5 87 292.0 88 292.0 89 292.0 90 185.0 91 78.0 92 78.0 93 78.0 94 49.0 95 20.0 96 20.0 97 20.0 98 15.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10911243547912981 2 0.04804204248707954 3 0.034362274456860284 4 0.03004663335208873 5 0.034036565694236014 6 0.019949661710736418 7 0.019786807329424286 8 0.023858166862227637 9 0.028092380776343122 10 0.034443701647516346 11 0.020682506426641024 12 0.018158263516302946 13 0.01473832150874813 14 0.021333923951889558 15 0.022311050239762365 16 0.022799613383698764 17 0.026626691344533916 18 0.019542525757456086 19 0.03289658502505108 20 0.022636759002386632 21 0.028336662348311323 22 0.03623509984194982 23 0.028255235157655254 24 0.03957361465884857 25 0.0308609052586494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1228091.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.70623716274464 #Duplication Level Percentage of deduplicated Percentage of total 1 87.47761710885851 68.85034078601586 2 8.99003654809616 14.151438973123973 3 1.9779911466771156 4.670407208885349 4 0.6608038577050668 2.080375405703662 5 0.30520439915534164 1.201074491151665 6 0.15527801420616938 0.733280892736248 7 0.09753905555892212 0.5373852427615455 8 0.06585152560811895 0.414634063363293 9 0.045849985521458414 0.3247811850924282 >10 0.20196914257945903 2.7479673462838643 >50 0.012674274485755815 0.6538886674900525 >100 0.007201839810970028 1.3668759611370807 >500 7.306164294320541E-4 0.3856880121551032 >1k 0.001252485307597807 1.8818617640999156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4461 0.36324669751671496 No Hit CCCATGTACTCTGCGTTGATACCAC 3371 0.2744910597016019 No Hit GTATCAACGCAGAGTACTTTTTTTT 2650 0.2157820552385776 No Hit GAGTACATGGGAAGCAGTGGTATCA 2266 0.18451401402664785 No Hit GGTATCAACGCAGAGTACTTTTTTT 1635 0.13313345672266957 No Hit TATCAACGCAGAGTACTTTTTTTTT 1445 0.11766229049801685 No Hit GTATCAACGCAGAGTACATGGGAAG 1374 0.11188095996143609 No Hit GCGTTGATACCACTGCTTCCCATGT 1236 0.10064400765089883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6285438131213403E-4 2 0.0 0.0 0.0 0.0 1.6285438131213403E-4 3 0.0 0.0 0.0 0.0 1.6285438131213403E-4 4 0.0 0.0 0.0 0.0 1.6285438131213403E-4 5 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 6 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 7 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 8 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 9 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 10 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 11 0.0 3.2570876262426806E-4 0.0 0.0 1.6285438131213403E-4 12 0.0 3.2570876262426806E-4 0.0 0.0 2.4428157196820105E-4 13 0.0 3.2570876262426806E-4 0.0 0.0 2.4428157196820105E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACGA 30 7.711672E-4 19.003586 2 TAGGACT 155 2.5465852E-11 11.647834 4 TCTATAC 130 3.2550815E-8 10.96316 3 CTACACT 210 0.0 10.859634 4 CCTACAC 140 8.3200575E-9 10.858749 3 CTAAGTC 70 0.0014891758 10.858749 3 GTAGGAC 280 0.0 10.858749 3 GCGTTTA 90 9.527589E-5 10.553678 13 CTCGAAC 120 1.5233327E-6 10.291093 18 TAGGACC 195 1.4551915E-11 10.233116 4 GTATTAA 160 6.035407E-9 10.106362 1 TTAGACC 105 4.095633E-5 9.953854 3 CTAAGAC 105 4.095633E-5 9.953854 3 TCCAGTA 115 1.0232774E-5 9.914914 2 TGTAGGA 355 0.0 9.903276 2 GTGCTAA 125 2.5267E-6 9.892344 1 GTATTAT 185 6.330083E-10 9.768948 1 GGCCTAG 110 6.738818E-5 9.511871 1 TAGTACA 150 2.635552E-7 9.50218 4 GTAAGAC 120 1.6959391E-5 9.501406 3 >>END_MODULE