FastQCFastQC Report
Thu 26 May 2016
SRR1513091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228091
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC50670.4125915750542916No Hit
CCCATGTACTCTGCGTTGATACCAC44010.3583610660773509No Hit
GTATCAACGCAGAGTACTTTTTTTT39810.3241616460018028No Hit
TATCAACGCAGAGTACTTTTTTTTT27930.22742614350239518No Hit
GGTATCAACGCAGAGTACTTTTTTT26820.21838772533957174No Hit
GAGTACATGGGAAGCAGTGGTATCA24010.1955066847652169No Hit
CATGTACTCTGCGTTGATACCACTG14910.12140794126819592No Hit
GTATCAACGCAGAGTACATGGGAAG14900.12132651407753986No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13730.11179953277078003No Hit
TATCAACGCAGAGTACATGGGAAGC13310.10837959076322519No Hit
GCTTCCCATGTACTCTGCGTTGATA13090.10658819256879172No Hit
ACGCAGAGTACATGGGAAGCAGTGG12860.10471536718370218No Hit
GCGTTGATACCACTGCTTCCCATGT12790.10414537684910971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC400.00533672314.2263610
TAGGACC1850.011.8341084
GTAATAC907.2950224E-611.6340153
GTGCGCC752.1013725E-411.38340711
GTGCAAT855.400038E-511.16020211
GTCCTAG1803.6379788E-1211.11611
GCGTGCG600.005936701311.0676519
TATACTG2000.010.946555
ATAAGTC700.001466697510.878565
CTAGAAC1252.1777305E-710.6609893
GGCGTGC1002.4349942E-510.4352138
ACAATAG1201.4842244E-610.3119683
TCTATAC1201.4842244E-610.3119683
TCTAGGA1701.4842954E-910.0860852
TAGGACA1909.640644E-1110.0197264
AAGGCGT951.6108208E-410.0197266
GTCCTAC2950.010.0125651
GTAGGAC2105.456968E-129.97201253
GTATAGA1659.720679E-99.8168161
TTAGGAC1856.2391337E-109.7760023
Produced by FastQC (version 0.11.5)