FastQCFastQC Report
Thu 26 May 2016
SRR1513090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124568
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27570.1297675574516796No Hit
GTCCTAAAGTGTGTATTTCTCATTT21730.10227961637377576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6050.012.4066354
GGACCGT550.003070848812.0899816
TCGAACT1701.8189894E-1211.73549819
GGACCGC658.0307195E-411.6914096
TCCAACG1552.5465852E-1111.64536318
TGCCGGT1104.9788287E-711.22588210
GCCGGTT1104.9818664E-711.22535311
GTCCTAA8000.011.0543551
GTATCAA21550.010.987341
GGCGAGG5450.010.98184219
TGGCGAG13750.010.84746118
TGGACCG803.7680147E-410.6886925
GGTATCA9750.010.6307121
AGGACCG909.507164E-510.5567335
GTTTAGG1659.1495167E-1010.3735881
CCAACGT1106.0461734E-610.36381719
TAGTACC1201.5215737E-610.2930574
AGGACGT12750.010.28355
GGCGTGC1303.848054E-710.2297428
ATGGCGA15400.010.17826917
Produced by FastQC (version 0.11.5)