FastQCFastQC Report
Thu 26 May 2016
SRR1513090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124568
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28730.135227490953455No Hit
GTCCTACAGTGGACATTTCTAAATT26770.12600208607114483No Hit
CTGTAGGACGTGGAATATGGCAAGA23130.1088691912897116No Hit
TATCAACGCAGAGTACTTTTTTTTT23110.10877505450519823No Hit
CACACACACACACACACACACACAC21760.10242082155054581No Hit
CTTTAGGACGTGAAATATGGCGAGG21740.10232668476603245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.013.1033751
TAGGACC6350.012.1427584
AGGACGT14500.011.2262365
TATACGG600.005788635511.1058765
GGACGTG14450.010.9356936
GAACAGT3600.010.8414516
TGTGCGG700.00151123310.84030310
AATCCCG1502.1846063E-910.75148619
GACGTGG6650.010.7009427
TAGGACG15400.010.6937854
ATAGGAC3650.010.6929383
TCCAACG909.625127E-510.543918
GGCGAGG6250.010.47321219
TGTAGGA16750.010.2909282
TTAGGAC10000.010.2808693
GTCCTAA7200.010.1876071
GACGTGA8500.010.1579227
GTAGGAC16250.010.1344183
ACGTGAA9550.010.1334838
TAAGACT3600.010.0481744
Produced by FastQC (version 0.11.5)